PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “ROSANILINE DYES”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 235 records · Page 13Linked to original sources
PubMed2000

A possible role of oxidative stress in the vanadium-induced cytotoxicity in the MC3T3E1 osteoblast and UMR106 osteosarcoma cell lines.

The cytotoxicity and free radical production induced by vanadium compounds were investigated in an osteoblast (MC3T3E1) and an osteosarcoma (UMR106) cell lines in culture. Vanadate induced cell toxicity, reactive oxygen species (ROS) formation and thiobarbituric acid reactive substances (TBARS) increased in a concentration-dependent manner (0.1-10 mM) after 4 h. The concentration-response curve of vanadate-induced cytotoxicity and oxidative stress in MC3T3E1 cells was shifted to the left of the UMR106 curve, suggesting a greater sensitivity of the non-transformed cells in comparison to the osteosarcoma UMR106 cells. Supplementing with vitamin E acetate (80 microM) significantly inhibited ROS and TBARS formation but did not improve the vanadate-dependent decrease in cell number. Other vanadium compounds (vanadyl, pervanadate, and VO/Aspi, a complex of vanadyl(IV) with aspirin) showed different degrees of cell toxicity and induced oxidative stress. Altogether these results suggest that oxidative stress is involved in vanadium induced osteoblastic cytotoxicity, although the mechanism is unknown.

Antioxidants↗
PubMed2006

Use of sentinel node mapping for cancer of the colon: 'to map or not to map".

Sentinel lymph node (SLN) mapping has become a cornerstone of oncologic surgery because it is a proven method for identifying nodal disease in melanoma and breast cancer. In addition, it can ameliorate the surgical morbidity secondary to lymphadenectomy. However, experience with SLN mapping for carcinoma of the colon and other visceral malignancies is limited. This study represents an update to our initial pilot experience with SLN mapping for carcinoma of the colon. Consenting patients over the age of 18 diagnosed with adenocarcinoma of the colon were included in this study. At the time of operation, 1 to 2 mL of isosulfan blue was injected with a 25-gauge needle into the subserosa at 4 sites around the edge of the palpable tumor. The SLN was identified visually and excised followed by a standard lymphadenectomy and surgical resection. SLNs were evaluated by standard hematoxylin and eosin (H&E) evaluation as well as immunohistochemical (IHC) techniques for carcinoembryonic antigen and cytokeratin if the H&E was negative. Sixty-nine patients underwent SLN mapping. A SLN was identified in 93 per cent (64 of 69) of patients. Nodal metastases were identified in 38 per cent (26 of 69) of patients overall. In 5 patients, the only positive node identified was the SLN, 2 of which were positive by IHC criteria alone. Therefore, 3 per cent (2 of 69) of patients were upstaged by SLN mapping. This technique was 100 per cent specific while being 46 per cent sensitive. Fourteen patients had false-negative SLNs. Metastasis to regional lymph nodes remains the key prognostic factor for colon cancer. SLN mapping is feasible for colon cancer and can identify a subset of patients who could benefit from adjuvant chemotherapy. Although SLN mapping did not alter the surgical management of colon cancer, it does make possible a more focused and cost-effective pathologic evaluation of nodal disease. We do not suggest routine utilization of SLN mapping for colon cancer, but we believe that the data supports proceeding with a national trial.

Adenocarcinoma↗
PubMed1989

Immunocytochemistry and image cytometry of progesterone receptors in breast carcinoma imprints. Expression of tissular and cellular heterogeneity: relations to biochemical assay, clinical and histological parameters and DNA analysis.

Breast cancer imprints from 93 patients were assayed for the presence of progesterone receptors (PgR) using a monoclonal antibody (Transbio) and an immunocytochemical assay (ICA) method which stains only the epithelial cell nuclei. Results were compared with conventional biochemical PgR determinations (DCCA) and were in qualitative agreement in 86% of the cases. Quantitative analyses were done on PgR-ICA- and Feulgen-stained imprints from 32 tumours using a SAMBA 2005 cell image processor. Results obtained showed a high correlation between DCCA values and the P product derived from the mean PgR concentration of marked tumour cells and the percentage of marked cells. Intra-tumoral and intra-cell heterogeneity were featured and showed relation to tumour differentiation and size.

Antibodies, Monoclonal↗
PubMed1999

Biodegradation of methyl violet by Pseudomonas mendocina MCM B-402.

Pseudomonas mendocina MCM B-402 was found to utilize a triphenylmethane dye, methyl violet as the sole source of carbon when incorporated in synthetic medium. Almost complete decolorization of methyl violet by P. mendocina was observed within 48 h of incubation at ambient temperature (28 +/- 2 degrees C) under aerated culture conditions, when the bacteria were inoculated into Davis Mingioli's synthetic medium at a concentration of 100 mg/l medium. Methyl violet was mineralized to CO2 through three unknown intermediate metabolites and phenol. The decolorization of the dye involved demethylation.

Biodegradation, Environmental↗
PubMed1976

Recent progress in the staining of DNA-aldehyde in cell nuclei.

The paper is a review of studies on Feulgen staining of nuclear DNA. For convenience, the paper is split into several sections, such as (a) the characteristic of the dyes used for the manufacture of Schiff reagent (b) preparations of Schiff reagent (c) fixation of the material (d) hydrolysis (e) staining with Schiff reagent (f) Schiff-type dyes and (g) use of Schiff-type dyes in quantitative cytochemistry. Barring the section on preparation of Schiff reagent that includes the classical methods, all other sections contain a comprehenside account showing progress made during the last 15 years. Under each section interpretations of new findings as far as possible, and in the section on staining with Schiff reagent, new approaches to its study have been presented. The section on Schiff-type dyes includes dyes with and without primary amino group.

Cell Nucleus↗
PubMed1980

Determination of gentian violet in animal feed, human urine, and wastewater by high pressure liquid chromatography.

Sensitive and specific procedures are described for the analysis of residues of gentian violet in animal feed, human urine, and wastewater at levels of 1000 ppm down to 10 ppb, 1 ppb, and 10 ppb, respectively. The cleaned-up extracts were analyzed by reverse-phase high pressure liquid chromatography by using an absorption detector at 588 nm. Recoveries of the compound from animal feed, human urine, and wastewater at the 10 ppb level were 79%, 58%, and 60%, respectively. Information concerning the stability of the compound in animal feed and the efficiency of extracting the residues is presented. Ancillary information is also reported concerning the separation and analysis of six related triphenylmethane dyes.

Animal Feed↗
PubMed1987

Effects of carbamoylation with alkyl isocyanates on the assay of proteins by dye binding.

The effect of carbamoylation with alkyl isocyanate was used both to monitor the stability of the isocyanates and to study the influence of charge modification on protein assay. Carbamoylation of poly (L-lysine) with methyl isocyanate, ethyl isocyanate and 2-chloroethyl isocyanate was observed to decrease binding of methyl orange. The data emphasized the lability of alkyl isocyanates and indicated the importance of preparing aqueous solutions at low temperatures for studies on protein carbamoylation. After carbamoylation of several proteins, there was decreased metachromasia on binding to Coomassie Blue G. Poly (L-lysine) and H1 histone showed anomalous behavior in that with low concentrations of Coomassie Blue G the metachromasia was increased by carbamoylation, but at high concentrations of the dye the metachromasia was decreased by carbamoylation. In contrast to some reports in the literature, the data indicated that there is not always a simple relationship between the positive charge on a protein and the interaction with anionic dyes.

Azo Compounds↗
PubMed1988

[Detection by the use of silver ions of additional protein zones in gradient polyacrylamide gels stained with Coomassie].

A simple method of revealing the additional zones of proteins in gradient polyacrylamide gels, preliminary dyed Coomassie by means of silver ions is described. The dyeing of Coomassie allows to avoid the time-consuming stages of preliminary treatment of gels as well to reveal more sensitive zones in gels. On the second stage of dyeing silver minor zones appear there which were not seen while Coomassie was dyed. The suggested method preserves high sensitivity characteristic of the methods of gel dyeing with silver.

Bacterial Proteins↗
PubMed1989

[Adsorption of dyes as an indicator of the biological activity of mineral dust].

A study is made on the adsorption capacity of several types silicates and quartz dusts, prepared from pure mineral standards with respect to methylene blue and fuchsin. There are differences in the adsorption capacity of the minerals referring to unit surface. The quantity of the adsorption for samples of the same mineral, broken to pieces of different dispersity, also differs. However, between the adsorption capacity of test samples, divided into 3 groups--quartz, zeolite and mixed from the work environment, with respect to methylene blue, and their biological aggressiveness, tested "in vivo" after intratracheal test and the subcutaneous pocket method and the zeolite dusts on diploid culture, an inverse dependence is established. The adsorption capacity of the test dusts from the work environment is many times higher than the examined quartz and zeolite dusts, because of the presence of mineral components in them, especially argillaceous, which have high affinity toward the dye. The inverse dependence established between the adsorption capacity with respect to methylene blue and the biological effect of the tested dusts give grounds to propose the adsorption capacity as one of the induces for studying and hygienic evaluation of different types dusts, which have no components manifesting specific affinity to the dye.

Adsorption↗