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PubMed1993

Chemotherapy of testicular cancer: 10-year experience.

A total of 250 patients with germ cell testicular tumors were treated by PVB chemotherapy between 1982 and 1992. Mean age of patients was 28.9 years (range 15-52). Thirty-four patients in clinical Stage II (11 patients IIA, 13 patients IIB, and 10 patients IIC) underwent primary retroperitoneal lymphadenectomy (RPL) with subsequent chemotherapy. They were followed-up for a mean of 106.3 months (range 85-125). CR was achieved in 30 patients (88.2%). Three patients relapsed. Twenty-seven patients (79.4%) are alive with no evidence of disease (NED) after a minimum of 5 years since the start of therapy. One hundred and twenty-two patients underwent primary chemotherapy for clinical Stages IM (15 patients), IIA (31 patients, IIB (48 patients) and IIC (28 patients) with RPL in cases with residual mass in the retroperitoneum. They were followed-up for a mean of 47.7 months (range 6-122). CR was achieved in 115 patients (92.7%) (75 of them received chemotherapy alone, 40 patients achieved CR following combined cytostatic-surgical treatment). Eleven patients relapsed. One hundred and nine patients (89.3%) are alive with NED. Ninety-four patients in Stages III and IV (8 patients III, 86 patients IV) underwent primary chemotherapy with additional surgical removal of residual metastases. They were followed-up for a mean of 50.5 months (range 6-125). CR was achieved in 65 patients (69.1%) (32 of them received chemotherapy alone, 33 patients achieved CR following combined cytostatic-surgical treatment). Eleven patients relapsed. Fifty-seven patients (60.6%) are alive NED. There were 11 patients with advanced germ cell testicular cancer (Stages IIC and IV) who underwent initial PVB chemotherapy without previous orchiectomy. Delayed orchiectomy was done simultaneously with surgical removal of residual mass in the retroperitoneum or in the lungs or at completion of chemotherapy alone. The toxicity of chemotherapy was moderate. There were drug-related deaths in ten patients (4%).

Adolescent↗

PubMed1996

Retroperitoneal lymphadenectomy: the traditional treatment option.

Retroperitoneal lymphadenectomy (RPL) is the traditional treatment option for clinical stage II low-volume disease. There are several issues to consider when making this treatment decision, including an understanding of staging accuracy and an appreciation of the associated morbidity of surgery versus chemotherapy. The surgeon must also know what type of RPL is indicated and whether nerve-sparing surgery is feasible. We also present the results from our institution for stage II nonseminomas.

Chemotherapy, Adjuvant↗

PubMed1996

Recurrent pregnancy loss: a review.

Recurrent pregnancy loss (RPL) is a frustrating problem for both the patient and the clinician. The causes of RPL are diverse and may be associated with genetic, anatomic, microbiologic, endocrine, or immunologic factors. When a couple is ready for an evaluation, the nurse practitioner needs to be able to discuss possible causes, aspects of diagnostic testing, and available options. Reassurance and clear information about prognosis are important and emotional support must be offered throughout the investigation and subsequent pregnancy.

Abortion, Habitual↗

PubMed1997

Recurrent pregnancy loss: a review.

Abortion, Habitual↗

PubMed1997

Incidence of autoantibodies in the infertile population.

The purpose of this study was to determine the incidence of autoantibodies in patients with no term pregnancies. Patients selected included 43 with primary infertility and 110 with a history of pregnancy loss. In the first group the incidence of antinuclear antibodies (ANA) and IgG and IgM antiphospholipid antibodies (APL) was 37.2% (p < 0.05) and 53.5% (p < 0.05), respectively. In the group of patients with a history of miscarriage, 31.8% (p < 0.05) were positive for ANA and 38.2% (p < 0.05) for APL. Controls were 35 healthy patients with proven fertility and no history of pregnancy loss or autoimmune disease. In this group the incidence of ANA was 5.7% and 11.4% for APL. The high incidence of autoantibodies found in patients with primary infertility might suggest a direct involvement of these antibodies in reproductive failure and consequently in IVF and assisted fertility procedures. The prevalence of ANA and APL has been extensively described in patients with a history of recurrent pregnancy losses (RPL). In this study we observed antibodies even after the first miscarriage. We therefore conclude that patients with a history of reproductive failure should be immunologically evaluated and treated before undergoing assisted fertilization techniques or before a new pregnancy in those cases of RPL.

Abortion, Spontaneous↗

PubMed2005

Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli.

The transcriptome profiles for wild-type (plasmid-free) and recombinant (plasmid-bearing) Escherichia coli during well-controlled synchronized high-cell-density fed-batch cultures were analyzed by DNA microarrays. It was observed that the growth phase significantly affected the transcriptome profiles, and the transcriptome profiles were significantly different for the recombinant and wild-type cultures. The response of the wild-type and recombinant cultures to an isopropyl-1-thio-beta-D-galactopyranoside- (IPTG-) addition was examined, where IPTG induced recombinant protein production in the plasmid-bearing cultures. The IPTG-addition significantly altered the transcriptome response of the wild-type cultures entering the stationary phase. The IPTG-induced recombinant protein production resulted in a significant down-regulation of many energy synthesis genes (atp, nuo, cyo), as well as nearly all transcription- and translation-related genes (rpo, rpl, rpm, rps, rrf, rrl, rrs). Numerous phage (psp, hfl) and transposon-related genes (tra, ins) were significantly regulated in the recombinant cultures due to the IPTG-induction. These results indicate that the signaling mechanism, associated with the recombinant protein production, may induce a metabolic burden in the form of a phage defense mechanism. Taken together, these results indicated that recombinant protein production initiated a cascade of transcriptome responses that down-regulated the very genes needed to sustain productivity.

Escherichia coli↗

PubMed1982

The mechanism of genetic resistance to Marek's disease in chickens.

Genetic resistance to Marek's disease in RPL line-6 chickens is expressed not only at the level of host immunological responses against virus an tumour antigens, but also at the level of target lymphoid cells for virus infection and transformation. The nature of the target cell involved was investigated. Spleen cells from susceptible line-7 chickens adsorbed more Marek's disease virus and turkey herpesvirus in vitro than line-6 spleen cells. In the case of Marek's disease virus this was reflected in the replicative ability of the virus in vivo. Transplantation of thymus fragments from 1-day-old line-7 chickens into thymectomized line-6 chickens conferred a high degree of susceptibility on the latter, but the transplantation of spleen or fragments had no significant effect. The reverse procedure, i.e. grafting of line-6 thymi into line-7 chickens, did not diminish the susceptibility of the recipients. In each treatment group the observed titres of leukocyte-associated viraemia correlated with the susceptibility of the group to Marek's disease. Histologically the grafted thymus fragments became depleted of lymphocytes immediately after transplantation. By 6 days there was substantial recovery, apparently as a result of re-population of the thymic epithelium by host stem cells. This was confirmed by transplanting thymus fragments between individuals of opposite sexes. Karyotype analysis showed that the thymus contained lymphocytes of the sex of the recipient. However, karyotype analysis of lymphoma cells taken from recipient line-6 chickens that had received thymus grafts from line-7 birds of the opposite sex showed that, in the majority of cases, the lymphomas consisted of cells of donor origin. It is concluded that the susceptibility of line-7 chickens is largely attributable to the greater susceptibility of their T-lymphocytes to infection and transformation by Marek's disease virus, and that this susceptibility can be transferred to genetically resistant line-6 birds by adoptive transfer of the cells in the form of thymus fragments.

Animals↗

PubMed1984

Partial characterization of a hepatocyte growth factor from rat platelets.

Rat serum has been shown to stimulate DNA synthesis in primary cultures of adult rat hepatocytes 2-3 times more potently than serum from several other mammalian sources, including humans. Parallel to its stimulation of thymidine incorporation into DNA, rat serum increased the total DNA content of the hepatocyte cultures over time, and also increased the frequency of nuclear labeling and mitosis. Moreover, normal rat serum, derived from whole blood (NRS), stimulated DNA synthesis in hepatocytes twice as effectively as platelet-poor rat serum, derived from plasma (ppNRS). Addition of a rat platelet lysate (RPL) to ppNRS restored the activity to equal that of NRS. The avid binding of the active principle to CM Sephadex and its sensitivity to trypsin digestion suggest that it is a cationic polypeptide with an apparent molecular weight of about 65,000, as determined by gel filtration. It was inactivated by reduction of disulfide bonds, or by exposure to pH below 5.5, to NaCl concentration below 0.05 M, to 65 degrees C for 30 min, or to 100 degrees C for 10 min. Although it resembles the human platelet-derived mitogen platelet-derived growth factor (PDGF) in several of its properties, it differs in others. Hence the hepatocyte growth factor from rat platelets, which accounts for 50% of the DNA synthesis-stimulatory activity of rat serum, appears to be a distinct entity.

Animals↗

PubMed2003

Hydrostatic brain edema: basic mechanisms and clinical aspect.

The term hydrostatic brain edema results from unfavorable hydrtostatic pressure gradiernts between blood vessels and brain tissue. Arterial hypertension combined with decompressive craniectomy produces extensive brain edema in the arterial boundary zone of the decompressive area. The increased hydrostatic pressure gradient enhances tissue damage and causes the biphasic opening of the blood-brain barrier (BBB). The hydrostatic pressure alone is capable of causing the initial BBB opening and this induced edema results in derangement of cerebral microcirculation and metabolism. With morphological opening and metabolic damage of the vascular wall, the second BBB opening is elicited by the amplified hydrostatic pressure gradient, which is similar to that in vasogenic edema. In the clinical aspect, the beneficial effect of decompressive craniectomy in the treatment of uncontrollable ICP and brain edema remains controversial. External decompression may have adverse effects on severe brain edema and swelling. In this regards, control of driving force for the formation of brain edema could be the treatment of choice as an initial step. In addition, recent reports provided by MR imaging indicated new information on the pathophysiological features of the patients with acute hypertension. In the patients with hypertension due to reversible posterior leucoencephalopathy (RPL) syndrome, MR images show reversible signal abnormalities in the bilateral occipital lobes, suggesting hydrostatic brain edema.

Animals↗

PubMed1976

A proposal for a uniform nomenclature for the genetics of bacterial protein synthesis.

A new genetic nomenclature for the macromolecules involved in bacterial protein synthesis is proposed and explained. Genes for ribosomal proteins are designated rsp, rpl and rpm while genes for ribosomal RNAs are rrs and rrl. Protein synthesis factors and ribosome assembly and modification activities are also consistantly named.

Bacterial Proteins↗

PubMed1983

Localization of concanavalin A binding sites in human pituitary adenoma cells as revealed by HRP-labelling method.

Intracellular lectin (Con A)-binding sites of human pituitary adenoma were examined by electron microscopy using the horseradish-peroxidase (HRP)-labelling technique. In this study were used 16 cases of human pituitary adenomas operated on in our clinics between 1977 and 1981: they concluded 4 each of PRL-, GH-, ACTH-producing and hormonal non-functioning adenomas. In parallel with the detection of lectin-binding sites, basal levels of their secreting hormones were determined by the radioimmunoassay technique, and their producing hormones were characterized light microscopically by the immunocytochemical HRP-labelling technique. In the present study, for hormonal functioning adenoma cells, mature or large granules of each specific type of adenoma cells had no definite Con A-binding sites. On the other hand, immature or small secretory granules of RPL- or GH-producing adenoma cells showed a positive reaction with Con A. ACTH-producing adenomas so far examined revealed no definite binding sites. Some variable results were obtained concerning non-functioning adenomas. Lectins have been and will be very useful in the detection of different subtypes of adenomas in each specific group.

Adenoma↗

PubMed1995

Identification and characterization of a cDNA clone derived from the Marek's disease tumour cell line RPL1 encoding a homologue of alpha-transinducing factor (VP16) of HSV-1.

We have identified and sequenced a 2.3 kb cDNA clone RPL(N.S) 6 derived from the Marek's disease virus (MDV)-transformed cell line RPL1, which contained open reading frames (ORFs) homologous to UL49 (VP22) and UL48 (VP16) of herpes simplex virus. Northern blot hybridization identified a 2.5 kb transcript corresponding to this cDNA clone in the total RNA from MSB1 lymphoblastoid cells, but not in RNA from the original RPL1 cells, most probably due to the very low level of its transcription. In vitro translation demonstrated that both MDV UL49 and UL48 can be expressed from a single mRNA.

Amino Acid Sequence↗

PubMed2005

Butyltin species in benthic and pelagic organisms of the Saguenay Fjord (Canada) and Imposex occurrence in common whelk (Buccinum undatum).

The distribution and accumulation of butyltins in various tissues of 13 benthic and pelagic species living in the sub-Arctic Saguenay Fjord (Canada) were investigated. Butyltin contamination was ubiquitous in this ecosystem with tributyltin (TBT) biota to sediment accumulation factors (BSAF) ranging between 0.22 and 11, but without any important biomagnification between trophic levels. The large range of butyltin compounds accumulating within different tissues of the species collected from all trophic levels was from 7 to 1238 ng Sn g(-1) d.w. and indicates an exceptional contamination level only found in northern coastal areas exposed to an intensive traffic of commercial ships. Results show that bioaccumulation in organisms depends on three main factors: (1) the actual contamination level in their habitat, (2) their assimilation pathway by water, sediment or diet, and (3) their ability to metabolize TBT and excrete metabolites. By their lack of an efficient TBT degradation system, bivalves are subject to accumulate more butyltins (from 890 to 993 ng Sn g(-1) d.w. for TBT and from n.d to 138 ng Sn g(-1) d.w. for metabolites) whereas most burrow-dwelling organisms are able to degrade TBT and their butyltin levels ranged from 86 to 239 ng Sn g(-1) d.w. for TBT and from 7 to 106 ng Sn g(-1) d.w. for metabolites. Acadian redfish (Sebastes fasciatus) feeding preferentially on shrimps and small crustaceans rich in TBT showed a contamination level about three times higher than eelpout (Licodes vahlii). The latter species living in contact with the sediment and feeding on worms and other burrowing species had a lower proportion of TBT in their tissues. Finally, deleterious effects of butyltins in the Saguenay Fjord were assessed by the significant occurrence of Imposex in common whelk (Bucinum undatum) in two sites from Baie des Ha! Ha!. Results revealed that the effects of Imposex were accentuated close to the source of contamination, at Port-Alfred harbour, since the total of imposexed whelks collected at site A (the mouth of Baie des Ha! Ha!) was 12.5% and reached 52.6% at site B (Port Alfred). Although the incidence or frequency of imposex was low in site A compared to site B, the relative penile length index (RPL) values, a measure of the degree or severity of imposex, was similar at both sites indicating the presence of TBT with higher concentrations in site B.

Animals↗

PubMed2005

Radiation-reducing planning of cardiac catheterisation.

Any radiation exposition for medical purposes should be kept as low as is reasonably achievable. Mean patient radiation exposure of diagnostic cardiac catheterisation is high (16-106 Gy x cm2) and for this reason the International Commission on Radiological Protection (ICRP) recommends credentialing radiation protection training programmes. Twenty cardiologists each documented various dose parameters of 10 cardiac catheterisations, before and after a 90-minute mini-course of the ELICIT study group ("Encourage to Less Irradiating Cardiologic Interventional Techniques"), and could achieve a reduction of the mean dose-area product by 15.9+/-9.0 Gy x cm2, equivalent to 47%. The presented radiation-reducing planning of invasive cardiac catheterisation for this reason is the first one validated in clinical routine and consists of 6 standard runs--one for the left ventricle, 3 and 2 for the left (LCA) and right coronary artery (RCA), respectively--depending on anatomy and findings supplemented by 1...4 special projections. The caudal posteroanterior (PA) view documents the left coronary main stem, proximal and distal left anterior descending artery (LAD), and proximal and mid circumflex segments. The cranial PA view however is suitable for the left coronary orifice, circumflex periphery, LAD, all diagonal bifurcations, and collateral pathways towards the RCA. LCA standard angiography is completed by lateral 90 degrees/0 degrees left anterior oblique (LAO) angulation. The 60 degrees/0 degrees LAO angulation visualises the right posterolateral artery (RPL) and the RCA to its bifurcation. The more proximal one finds the bifurcation, the more the second standard cranial PA view for RCA should vary towards the cranial right anterior oblique (RAO) and finally 30 degrees/0 degrees RAO view. The efficiency of these less-irradiating angulations are improved by radiation-reducing techniques as follows: restriction to essential radiographic frames and runs, consistent collimation to the region of interest--particularly during coronary intubation--, adequate instead of best possible image quality, short skin-to-image-intensifier distance, inspiration during radiography, preference for projections that rotate out the spine, optimisation of fluoroscopy time, well-experienced and well-rested interventionists.

Body Burden↗

PubMed1998

Further characterization of the latency-associated transcription unit of Marek's disease virus.

Previous studies have identified a large (L) and a small (S) RNA transcript antisense to the MDV homologue of the ICP4 gene of herpes simplex virus (HSV) in cells infected with Marek's disease virus (MDV) and in lymphoblastoid cell lines. In this study the 5' and 3' ends of the L RNA and of the sense ICP4 transcript of MDV were mapped by Northern hybridization and RNase protection assays. The results showed that L RNA is approximately 10.6 kb and that the ICP4 sense transcript is initiated in the region of genomic DNA where the L RNA terminates whereas L RNA is initiated where the ICP4 transcript terminates. L RNA was abundant in chick embryo fibroblasts (CEF) infected with virus strain HPRS16/attenuated whereas S RNA was predominant in CEF infected with oncogenic HPRS16 and in RPL-1 cell line. Results of cycloheximide experiments showed that the ICP4 gene of MDV was transcribed as an immediate-early gene in infected CEF whereas transcription of the L RNA required protein synthesis. Sequencing of cDNA and Northern hybridization using oligonucleotide probes showed that S RNA shared similar intron/exon boundaries as the cDNAs from several cell lines indicating that there might be a relationship between the S RNA and the antisense transcripts that generated the cDNAs.

Animals↗

PubMed1991

In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein-RNA polymerase gene cluster of Escherichia coli.

Transcription of the rplKAJLrpoBC ribosomal protein (rpl) RNA polymerase (rpo) gene cluster is governed by a complex set of signals. To dissect the transcription units active in vivo and to quantify the relative contribution of each, an extensive array of rplKAJLrpoB/lacZ gene fusions were constructed on lambda phage derivatives and introduced in single copy into the chromosomes of lac- cells. Measurements of beta-galactosidase production from fusions containing wild-type and/or mutagenized rplrpo DNA fragments permitted the establishment of high-resolution transcription profiles of the gene cluster. The results show that transcription initiated at the upstream rplKp promoter (located just before rplK) does not terminate before the rplJp promoter (located upstream from rplJ), but instead reads through into the distal genes. In addition, rplJp continues to function efficiently in the presence of readthrough transcription from rplKp. As a result the rplJL genes are transcribed at almost twice the frequency of the upstream rplKA genes. However, the transcription of rpoB, which is situated downstream from the previously identified attenuator (rpoBa), is only marginally increased (20%) when both promoters are present. This suggests that although both transcription units overlap, transcriptional termination at rpoBa is modulated in response to the frequency of initiation from both promoters.

Bacteriophage lambda↗

PubMed1984

Morphological transformation, autonomous proliferation and colony formation by chicken heart mesenchymal cells infected with avian sarcoma, erythroblastosis and myelocytomatosis viruses.

Normal chicken heart mesenchymal cells at low density in monolayer culture in plasma-containing medium have a polygonal shape and are proliferatively quiescent. The combination of epidermal growth factor and insulin at hyperphysiological concentration, an insulin-like growth factor surrogate, causes these cells to assume a fusiform shape and to increase 40-fold in number during four days of incubation. These mitogenic hormones do not, however, induce normal chicken heart mesenchymal cells to form colonies in agarose suspension culture. Chicken heart mesenchymal cells infected with the Schmidt-Ruppin or Prague-A strains of Rous sarcoma virus or with the Fujinami or Y73 avian sarcoma viruses assume spindle and round shapes, increase 50-100 fold in number during four days of monolayer culture in the absence of mitogenic hormones and form macroscopic colonies during 3-4 days of agarose suspension culture. The autonomous (mitogenic hormone-independent) proliferation, in monolayer culture, of cells infected with temperature-sensitive transformation mutants of Rous sarcoma virus (tsNY68, tsNY72, tsLA24, tsLA29) is temperature-sensitive. Chicken heart mesenchymal cells infected with avian erythroblastosis virus assume spindle shapes and proliferate in monolayer culture at a rate comparable to that of sarcoma virus-infected cells but do not, however, form colonies in agarose suspension culture. Cells infected with the myelocytomatosis virus MC29 assume stellate shapes and increase 18-fold in number during four days of monolayer culture. Cells infected with the myelocytomatosis virus MH2 assume fusiform shapes and increase fourfold in number during four days of monolayer culture. Neither MC29 nor MH2 renders chicken heart mesenchymal cells capable of colony formation in agarose suspension culture. Infection with avian leukosis viruses (RAV-1, RAV-2, RPL-42) or with transformation-defective mutants of Rous sarcoma virus (tdNY105, 107, 109) does not affect the morphology or proliferative behavior of chicken heart mesenchymal cells. Monolayer culture of chicken heart mesenchymal cells in plasma-containing medium appears, therefore, to define the ability of onc genes of acute transforming avian retroviruses to induce autonomous (mitogenic hormone-independent) cell proliferation, the essential characteristic of neoplasia. The differences in transformed morphology and rates of autonomous proliferation between cells infected with different acute transforming retroviruses probably reflects differences in the modes of action of the transforming proteins encoded by the onc genes of the respective viruses.(ABSTRACT TRUNCATED AT 400 WORDS)

Alpharetrovirus↗

PubMed1992

Viscoelastic properties of the visceral pleura and its contribution to lung impedance.

The mechanical impedances of 10 dog lung lobes (ZL) and circular pleura samples 1.6 cm in diameter (Zpl) were measured with small-amplitude forced oscillations between 0.2 and 4.2 Hz. Two to four samples were ablated from each lobe after their in situ tension had been fixed at 5 cmH2O transpulmonary pressure with plastic rings. Lobe resistance was inversely proportional to frequency (f) and lobe elastance increased linearly with the logarithm of f by 23%/decade. The real part of Zpl (Rpl) decreased hyperbolically with f. Pleural elastance (Epl) showed only a 5%/decade increase with log f. The regional variability of Epl was large and the data allowed the lumping of Epl into only two groups. The variability of Epl was higher in the group of Epl values from the coastal surfaces than in the group of Epl values from any other surfaces (3105 +/- 2741 (SD) vs 2263 +/- 1152 cm H2O/L). The mean intraindividual variation of Epl corresponding to costal and to other surfaces was 38 +/- 25 and 32 +/- 23%, respectively. The hysteresivity index (Fredberg and Stamenovic, J. Appl. Physiol., 67: 2408-2419, 1989) of the pleura was significantly smaller than that of the lobes (0.025 vs 0.148). Extrapolation of Zpl to the entire lobe surface predicted pleural/lobar resistance and elastance ratios of 2.7 +/- 0.4 and 16.8 +/- 10.6%, respectively, at 0.2 Hz, and 0.4 +/- 0.4 and 15.5 +/- 10.6%, respectively, at 4.2 Hz. This suggests that for small deformations and medium lung volumes the pleural contribution to ZL is almost ideally elastic and only slightly frequency-dependent.

Airway Resistance↗