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At least 235 records · Page 13Linked to original sources

PubMed2005

The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus.

The total haemocyte count (THC), phenoloxidase activity, respiratory burst, superoxide dismutase (SOD) activity, phagocytic activity and clearance efficiency to the pathogen Vibrio alginolyticus were examined in the white shrimp Litopenaeus vannamei (10.3+/-1.5 g) injected individually with hot-water extract of Gracilaria tenuistipitata at 4 or 6 microg g-1. L. vannamei receiving hot-water extract of G. tenuistipitata at either dose increased significantly its THC, phenoloxidase activity, and respiratory burst after 2 days. L. vannamei received hot-water extract of G. tenuistipitata at 6 microg g-1 increased its phagocytic activity and clearance efficiency to V. alginolyticus after 1 day. In another experiment, L. vannamei which had been injected with hot-water extract of G. tenuistipitata were challenged with V. alginolyticus at 2x10(6) colony-forming units (cfu) shrimp-1 and then placed in seawater of 34 per thousand. The survival of shrimp that received hot-water extract of G. tenuistipitata at 6 microg g-1 was significantly higher than that of shrimp that received saline and the control shrimp after 3 days, and at the termination of the experiment (6 days after the challenge). It is therefore concluded that L. vannamei receiving the hot-water extract of G. tenuistipitata at 6 microg g-1 or less increased its immune ability and resistance to V. alginolyticus infection.

Animals↗

PubMed2006

Protection of shrimp (Penaeus chinensis) against white spot syndrome virus (WSSV) challenge by double-stranded RNA.

To determine whether Penaeus chinensis can be protected against white spot syndrome virus (WSSV) infection by intramuscular injection with long double-stranded RNAs (dsRNAs) as in other shrimp species and whether the protection degree by WSSV-specific dsRNAs is correlated with the roles of viral genes, P. chinensis juveniles were intramuscularly injected with long dsRNAs corresponding to VP28, VP281, protein kinase genes of WSSV, and an unrelated long dsRNA corresponding to a green fluorescence protein (GFP) gene. All shrimp injected with long dsRNAs including GFP dsRNA showed higher survival rates against WSSV infection than shrimp injected with PBS alone. Furthermore, shrimp injected with dsRNAs corresponding to VP28 and protein kinase showed higher survival rates than those injected with dsRNAs corresponding to VP281 and GFP. These results indicate that the introduction of long dsRNAs corresponding to viral proteins, which are essential for WSSV infection, is quite effective in blocking WSSV infection in P. chinensis, and suggest that dsRNA-mediated protection is a common feature across shrimp species.

Animals↗

PubMed2006

Immunostimulation of white shrimp (Litopenaeus vannamei) following dietary administration of Ergosan.

Ergosan an algal product containing 1% alginic acid, developed for use in aquaculture and reported to have immunomodulatory activity, was administered orally to intermoult adult white shrimp (Litopenaeus vannamei) for 15 days. Examination of haemolymph proteins using SDS-PAGE did not reveal any obvious differences between control and Ergosan treated shrimp. Similarly, total haemocyte counts were found to be roughly equivalent for both the control and experimental samples. However, differential analysis of haemocyte populations revealed marked changes in terms of the relative levels of hyaline, semi-granular, and particularly granular haemocytes between the two groups. Moreover, enhancement of the in vitro antimicrobial activity of haemolymph towards two shrimp pathogenic Vibrio isolates was recorded for shrimp fed with Ergosan. Finally, shrimp fed with Ergosan showed a significant increase in relative growth when compared with control groups.

Alginates↗

PubMed2004

Assessing the influence and distribution of shrimp pond effluent in a tidal mangrove creek in north-east Australia.

Effluent from a land based shrimp farm was detected in a receiving creek as changes in physical, chemical and biological parameters. The extent and severity of these changes depended on farm operations. This assessment was conducted at three different stages of shrimp-pond maturity, including (1) when the ponds were empty, (2) full and (3) being harvested. Methods for assessing farm effluent in receiving waters included physical/chemical analyses of the water column, phytoplankton bioassays and nitrogen isotope signatures of marine flora. Comparisons were made with an adjacent creek that served as the farms intake creek and did not directly receive effluent. Physical/chemical parameters identified distinct changes in the receiving creek with respect to farm operations. Elevated water column NH(4)(+) (18.5+/-8.0 microM) and chlorophyll a concentrations (5.5+/-1.9 microg/l) were measured when the farm was in operation, in contrast to when the farm was inactive (1.3+/-0.3 microM and 1.2+/-0.6 microg/l, respectively). At all times, physical/chemical parameters at the mouth of the effluent creek, were equivalent to control values, indicating effluent was contained within the effluent-receiving creek. However, elevated delta(15)N signatures of mangroves (up to approximately 8 per thousand) and macroalgae (up to approximately 5 per thousand ) indicated a broader influence of shrimp farm effluent, extending to the lower regions of the farms intake creek. Bioassays at upstream sites close to the location of farm effluent discharge indicated that phytoplankton at these sites did not respond to further nutrient additions, however downstream sites showed large growth responses. This suggested that further nutrient loading from the shrimp farm, resulting in greater nutrient dispersal, will increase the extent of phytoplankton blooms downstream from the site of effluent discharge. When shrimp ponds were empty water quality in the effluent and intake creeks was comparable. This indicated that observed elevated nutrient and phytoplankton concentrations were directly attributable to farm operations.

Animals↗

PubMed2004

Seed supply for coastal brackishwater shrimp farming: environmental impacts and sustainability.

Globally, shrimp farming has been a significant agro-based economic activity since the early 1970s. Because it offered a huge immediate economic return, shrimp farming showed a booming expansion and soon became a multimillion dollar industry. However, it has been under extreme criticism because of its devastating ecological and socio-economic impacts. Because seed is the primary input, the impact from farming has started from the source of seed supply, so that not only are natural stocks of shrimp seeds now overexploited worldwide but seed collection activities also significantly reduce stocks of other living resources. Although hatcheries were developed as potential alternative and have replaced the natural seed source to a great extent, large-scale hatchery productions provide a potential source of coastal pollution. However, this area is still poorly studied. The present paper provides a review of the environmental impacts of the wild shrimp seed fishery as well as the possibility of environmental degradation from artificial shrimp seed production in hatcheries.

Animals↗

PubMed2003

Comparative immunohistochemistry and cellular distribution of farnesoic acid O-methyltransferase in the shrimp and the crayfish.

Farnesoic acid O-methyltransferase (FAMeT) catalyzes the conversion of farnesoic acid (FA) to methylfarnesoate (MF) by the mandibular organ (MO) of crustaceans. Here we report the cellular localization of FAMeT and radiochemical assay of endogenous FAMeT activity in shrimp (Metapenaeus ensis) and crayfish (Procambarus clarkii) tissues. As in the eyestalk (ES), FAMeT is concentrated in specific neurosecretory cells of the ventral nerve cord (VNC) whereas only weak FAMeT immunoreactivity was observed in the MO. FAMeT was also detected in the ventral nerve cord, heart (HET), eyestalk, and muscle of the juvenile shrimp. Although the VNC shows the greatest FAMeT immunoreactivity, the heart extract exhibited the highest FAMeT enzymatic activity. These results suggest that FAMeT in the VNC may be inactive or inactivated at the stages of development tested. Contrary to the previous reports in other crustaceans, MO extract in shrimp shows only low FAMeT activity. The eyestalk, epidermis, ovary and testis show appreciable FAMeT activity. The presence of FAMeT in neurosecretory cells of VNC and eyestalk of shrimp and crayfish implies a possible interaction of FAMeT with the eyestalk CHH-family of neuropeptides. The widespread activity of FAMeT suggests that it has a wide spectrum of action in many tissues that contribute to the function and regulation of MF synthesis in shrimp and crayfish.

Animals↗

PubMed2005

Distribution of micro-essential (Fe, Cu, Zn) and toxic (Hg) metals in tissues of two nutritionally distinct hydrothermal shrimps.

Hydrothermal ecosystems of the Mid Atlantic Ridge (MAR) are dominated by shrimps and mussels that are naturally exposed to elevated levels of heavy metals providing unique in situ laboratories for ecotoxicological investigations. This study reports on the tissue compartmentalization of both micro-essential (Fe, Zn, Cu) and toxic metals (Hg) in two nutritionally distinct cariddean vent shrimps: Rimicaris exoculata and Mirocaris fortunata, in order to shed light on organism-biota interactions at hydrothermal vents. High metal concentrations in shrimps confirmed extreme exposure levels at both geochemically different hydrothermal vents (Rainbow and Lucky Strike). However, Hg concentrations were below those reported in species for human consumption that may either suggest low bioavailability of the metal, or its effective detoxification/depuration by the hydrothermal shrimp that needs to be confirmed by post-capture toxicological investigations. Distribution of metals in different tissues had very similar patterns in both shrimp species, the target organs being gill and pylorus. Tissue levels correlated well with end-member fluid composition with regard to element ratios, i.e. Fe/Zn and Fe/Cu ratios in end-member fluids reported for Rainbow and L. Strike were conserved in the gills of M. fortunata. Moreover, R. exoculata that lives closer to venting exits as compared to M. fortunata, had similar or often less metals accumulated in selected organs, possibly owing to its higher degree of adaptation to hydrothermal conditions. Despite of high concentrations in tissues micro essential metals only represented a small fraction (14-36%) of the whole body burden indicating preponderance of minerals on the surface and/or in the gut, and thus points to improper use of whole body concentration in metal bioavailability interpretations at hydrothermal vents.

Animals↗

PubMed2006

Binding of shrimp cellular proteins to Taura syndrome viral capsid proteins VP1, VP2 and VP3.

Viruses are a major cause of production losses in the world shrimp-farming industry. Despite this, little is known about viral-host interactions in shrimp due in part to the lack of continuous shrimp cell lines. Here, the yeast two-hybrid assay system was employed to study interactions between three Taura syndrome viral capsid proteins (VP1-VP3) and proteins from a cDNA library of the black tiger shrimp Penaeus monodon. VP1 interacted with beta-actin, elongation factor 1alpha (EF1alpha), lysozyme (Lys) and laminin receptor/ribosomal protein p40 (Lamr/p40) containing a putative palindromic laminin binding region LMWWML. VP2 interacted with beta-actin and EF1alpha, while VP3 bound to the same proteins as VP1 except for Lamr/p40. In vitro pull-down assays confirmed these interactions. The most interesting interaction was specific binding between VP1 and Lamr/p40 since Lamr/p40 has been identified as the mammalian cell receptor for several arthropod-borne viruses (arboviruses). A search of mosquito vector and Drosophila sequences at available databases revealed the presence of putative Lamr/p40 proteins with high homology to the Lamr/p40 from shrimp.

Actins↗

PubMed2000

Cell surface NADH oxidase activity of brine shrimp oscillates with a period of 25 min and is entrained by light.

Plants have a surface NADH oxidase that measures time by oscillating with a 24-min period. The period is synchronized by light. With plants, a new maximum is observed exactly 12 min after the beginning of the light exposure. These experiments were to determine if animals exhibited a cell surface NADH oxidase having a similar periodicity and to answer the question, does the periodicity in animals respond to light? Using brine shrimp as a model, the findings show that plants and animals exhibit similar oscillating NADH oxidase activity and that the periodicity in this invertebrate animal does respond to light. Brine shrimp were grown for two to three days and transferred to darkness for 45 min. After return to light for one min, NADH was added and measurements of NADH oxidation were recorded over 50 min. The brine shrimp exhibited a cell surface NADH oxidase that oscillated with a period of 25 min. After being subjected to light, the brine shrimp showed a new maximum in NADH oxidation between 12 to 13 min after the beginning of the light exposure and again at 37 min and at 25 min intervals thereafter. The findings demonstrate that the periodic oscillations in NADH oxidation of brine shrimp are light entrainable.

Animals↗

PubMed2003

A field survey of chemicals and biological products used in shrimp farming.

This study documented the use of chemicals and biological products in marine and brackish water shrimp farming in Thailand, the world's top producer of farmed shrimp. Interviews were conducted with 76 shrimp farmers in three major shrimp producing regions, the eastern Gulf coast, the southern Gulf coast and the Andaman coast area. Farmers in the study used on average 13 different chemicals and biological products. The most commonly used products were soil and water treatment products, pesticides and disinfectants. Farmers in the southern Gulf coast area used a larger number of products than farmers in the other two areas. In the study, the use of more than 290 different chemicals and biological products was documented. Many of the pesticides, disinfectants and antibiotics used by the farmers could have negative effects on the cultured shrimps, cause a risk for food safety, occupational health, and/or have negative effects on adjacent ecosystems. Manufacturers and retailers of the products often neglected to provide farmers with necessary information regarding active ingredient and relevant instructions for safe and efficient use.

Animals↗

PubMed2003

Shrimp aquaculture development and the environment in the Gulf of California ecoregion.

Beginning in the middle of the 1980s, the Gulf of California ecoregion experienced a boom in shrimp aquaculture and became the second largest producer in the western hemisphere. The moderated, but continual development of shrimp farming, in conjunction with municipal and agriculture effluents has been accompanied by concern about: (a) depletion of fishing stocks, (b) reduction of mangrove forest, (c) frequent harmful algal blooms in coastal waters and shrimp ponds, and (d) water quality deterioration. We demonstrate that environmental degradation resulted from a conjunction of factors including agriculture, untreated municipal effluents, shrimp aquaculture, increasing number of fishermen, and an absence of an effective regulatory program. We recommend the immediate implementation of an integrated coastal management program to protect the integrity of the coastal ecosystems and operate upon the principle of environmental sustainability for the different economic activities including shrimp aquaculture.

Animals↗

PubMed1997

Quantification of the major brown shrimp allergen Pen a 1 (tropomyosin) by a monoclonal antibody-based sandwich ELISA.

BACKGROUND: Among 13 allergens found in extracts of cooked brown shrimp (Penaeus aztecus) the 36 kd muscle protein tropomyosin has been identified as the only major shrimp allergen (Pen a 1). Cross-reacting molecules with similar molecular weights were detected in other crustacea species such as crab, lobster, and crawfish. Because Pen a 1 and Pen a 1-like allergens are important in crustacea allergy, the aim of this study was to develop a monoclonal antibody (mAb)-based sandwich ELISA to quantify Pen a 1 and to evaluate Pen a 1 levels in four commercial shrimp, crab, and lobster extracts. METHODS: Two Pen a 1-specific mAbs with different epitope specificities were selected. ELISA plates coated with captured mAb 3.2 were incubated with samples containing Pen a 1. Bound Pen a 1 was detected by a combination of biotinylated mAb 4.9.5 and alkaline phosphatase-labeled streptavidin. RESULTS: The optimized sandwich ELISA could detect Pen a 1 concentrations ranging from 4 to 125 ng/ml. Four commercial shrimp extracts demonstrated a 40-fold difference in Pen a 1 levels (24 to 920 microg/ml). Crab and lobster extracts contained detectable levels of Pen a 1-like proteins. No reactivity to cockroach, house dust mite, oyster, codfish, or peanut extracts was detected, which indicates that the developed assay is crustacea-specific. CONCLUSION: A sensitive sandwich assay was developed to quantify Pen a 1. This assay will be helpful to standardize shrimp extracts in regard to the content of the major allergen, Pen a 1, and to study cross-reactivities among and evaluate occupational exposure to different crustacea species.

Allergens↗

PubMed2000

Trophic accumulation and depuration of mercury by blue crabs (Callinectes sapidus) and pink shrimp (Penaeus duorarum).

Mercury concentrations in blue crabs (Callinectes sapidus) collected from an area of mercury-contaminated sediments in Lavaca Bay, TX, USA, are more than an order of magnitude greater than concentrations in penaeid shrimp from the same area. Laboratory feeding experiments using mercury-contaminated fish as food showed that both blue crabs and pink shrimp (Penaeus duorarum) could accumulate mercury concentrations comparable to those in their food in 28 days. Calculated mercury assimilation efficiencies averaged 76% for blue crabs and 72% for pink shrimp. Significant depuration of mercury by blue crabs was not observed during a subsequent 28-day period, but pink shrimp lost mercury at a rate of about 0.012 day-1. Model calculations predict biomagnification factors of mercury of about two to three at steady state for both species. The large difference in observed concentrations of mercury in field-collected blue crabs and penaeid shrimp does not result from differences in efficiency of mercury assimilation from their food or from differences in excretion rates. It is more likely the result of differences in residence times in the contaminated area and of differences in feeding habits.

Animals↗

PubMed2002

A beta-1,3-glucan binding protein from the black tiger shrimp, Penaeus monodon.

A beta-1,3-glucan binding protein (GBP) has been isolated from a shrimp hemocyte cDNA library. Its open reading frame consists of 1314 nucleotides with a polyadenylated sequence and a poly A tail. It encodes a polypeptide of 370 amino acids including a 17 amino acid-signal peptide. The mature protein has an estimated molecular mass of 39.5 kDa and a predicted pI of 5.5. Sequence comparison shows a high degree of similarity to invertebrate recognition proteins with glucanase-like domains for example, the lipopolysaccharide- and beta-1,3-glucan-binding protein (LGBP) from the freshwater crayfish, Pacifastacus leniusculus, coelomic cytolytic factor-1 from the earthworm, Eisenia foetida and the Gram negative bacteria binding protein from the mosquito, Anopheles gambiae as well as to sea urchin beta-1,3-glucanases and bacterial beta-1,3-glucanases or beta-1,3-, 1,4-glucanases. Northern blot analysis showed that the shrimp protein is constitutively expressed in hemocytes. Animals injected with curdlan or heat-killed bacterial cell of Vibrio harveyi, a shrimp pathogen, showed no significant change in the mRNA expression profile within 12h post-injection. After incubation of shrimp hemocyte lysate supernatant (HLS) with curdlan or zymosan, a protein with a molecular mass of 31 kDa was eluted from the incubated curdlan or zymosan, and, by immunoblotting, this 31-kDa band could be detected by an affinity-purified anti-crayfish LGBP antibody. In contrast, incubation of shrimp HLS with LPS showed no any reactive band detected on SDS-PAGE or by immunoblotting suggesting that the binding is specific for beta-1,3-glucan.

Amino Acid Sequence↗

PubMedDate not supplied

The prophenoloxidase activating system of the shrimp Penaeus paulensis and associated factors.

In the present study we investigated the proPO activating system of the penaeid Penaeus paulensis, focusing on its role in the shrimp immune system. The great majority of PO activity (more than 90%) was found in the shrimp hemocytes. The enzyme activity was greatly enhanced by components of microorganism cell walls, such as LPS and beta-1,3-glucans, suggesting its involvement in non-self recognition. PO activity was also found in the shrimp serum and trypsin, and LPS were able to increase the enzyme activity. Thus, serum can be used as an alternative for the study of the shrimp proPO activating system, as it is much more readily obtained than HLS. PO activity was cation-dependent, and 5 mM of calcium and 10 mM of magnesium were the optimal concentrations for the enzyme activity. An immune factor was found in the shrimp HLS, capable of inducing cell-adhesion and degranulation of the penaeid hemocytes.

Animals↗

PubMed1997

A new RNA-friendly fixative for the preservation of penaeid shrimp samples for virological detection using cDNA genomic probes.

In situ hybridization analysis of shrimp histological sections, utilizing Taura syndrome virus (TSV) specific cDNA probes, is the most sensitive diagnostic technique presently available for the detection of this penaeid shrimp viral disease. However, false negative genomic probe results are obtained frequently from samples of Pacific white shrimp, Penaeus vannamei, that have been preserved with Davidson's AFA (acetic acid, formaldehyde, alcohol) fixative and that, otherwise, demonstrate pathognomonic TSV lesions by routine histology. This problem was linked to prolonged storage of shrimp samples in Davidson's fixative, which is highly acidic (pH approximately 3.5-4). Degradation of TSV genomic RNA was hypothesized to be due to either fixative- induced acid hydrolysis and/or acidophilic endogenous ribonuclease activity. Routine H and E histology and in situ hybridization analyses were conducted on equal numbers of TSV infected P. vannamei juveniles that were preserved for four different time periods (2, 6, 10 and 14 days) with either Davidson's fixative or a new, near neutral (pH approximately 6.0-7.0), RNA-friendly fixative (R-F) that was developed by the authors. In situ hybridization assays were conducted with and without R Nase precautions and all of the samples tested contained moderate to severe TSV lesions by routine histology. Davidson's preserved samples produced weak TSV probe signals after 2 days fixation, but did not react with the probes in those samples that were stored for > 6 days in the fixative. In contrast, TSV was detectable by gene probe in all of the time treatment samples preserved with the new R-F fixative. Equivalent in situ hybridization results were obtained when the same samples were analyzed in the absence of RNase-free conditions. These findings suggest that TSV RNA is degraded when samples are stored in an acidic fixative, such as Davidson's, for more than 2 days and that this problem can be prevented through preservation of shrimp samples with R-F fixative. The efficacy of this new fixative is demonstrated and the results show that RNase-free conditions are not necessary for conducting TSV in situ hybridization analyses.

Animals↗

PubMed2002

Problems and solutions with the design and execution of an epidemiological study of white spot disease in black tiger shrimp (Penaeus monodon) in Vietnam.

White spot disease (WSD) is caused by white spot syndrome virus (WSSV) and is an acutely fatal pandemic disease of crustaceans. It has resulted in massive losses to the shrimp-farming industry in Asia and has now spread to the Americas. This paper reports the problems and solutions associated with the design and execution of a longitudinal epidemiological study of shrimp (Penaeus monodon) health on farms practising a crop rotation of rice and shrimp in the Mekong Delta of Vietnam. The pre-sampling phase of the project involved selecting an appropriate site and sampling variables, obtaining permission and establishing the necessary laboratory and logistic facilities. At the start of the sampling phase, 40 farmers were selected and 32 of these were visited and interviewed. This resulted in the enrolment of only 17 farmers. A further seven had to be enrolled to obtain the maximum number of farmers that could be sampled by the study team. Compliance was enhanced through meetings, regular visits by senior members of the project team and ensuring that visits were punctual and that all information was treated confidentially. The production cycle began in January 1998 and lasted for approximately 5 months. An attempt was made to collect 500 post larvae (PL) before each pond was stocked to assess the health of the batch and to test for the presence of WSSV by one-step PCR. After stocking, the wild crustaceans also were sampled from the pond for PCR analyses. Information was collected on the management practices and samples of water, pond bottom, feed and shrimp collected throughout the production cycle. Water quality variables with predictable diurnal variation were sampled in the morning and afternoon, twice a week. Two months after stocking, the first outbreak of WSD occurred; subsequently, 18 farms conducted a complete emergency harvest due to the actual or perceived presence of a WSD outbreak. Detectable mortalities were reported from 19 farms, and moribund shrimps were collected from four of these for PCR and histological analyses.

Animals↗

PubMed1998

Microbiological quality of Icelandic cooked-peeled shrimp (Pandalus borealis).

Iceland is a major producer of cold water shrimp, Pandalus borealis. In recent years considerable attention has been given to improve hygiene in the factories producing cooked, peeled and frozen shrimp. To keep track of the bacteriological status of the end product, shrimp from most of the factories is routinely analysed bacteriologically by the request of shrimp exporters. This paper reports on the results of a bacteriological analysis of 7913 samples of shrimp from 26 Icelandic factories over a 6-year period. The results showed that the geometric mean of APC (at 35 degrees C) was 1718 per g and 57% of the samples had APC under 1000 per g. Some 70% of the samples had less than one coliform per g and 99.9% of the samples had less than one faecal coliform per g. Staphylococcus aureus was detected in less than 0.2% of the samples. The results show improvement in bacterial profiles, mainly total coliforms, over the 6-year period. Overall, the results show acceptable bacterial numbers in the finished product, indicating a high level of hygiene. Listeria spp. were, however, found in 270 of 3331 samples examined or 8.1%. Species identification was done on 49 of the 270 positive samples. The proportion of L. monocytogenes was found to be 26.5%.

Animals↗