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PubMed1997

[The interim report on survey of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in marine products and estimation of exposure through fishes and shellfishes].

Survey of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans has been carried out for four years starting from 1992 to 1995, which showed that about 25% or more of marine products are contaminated by trace amount of dioxins and related compounds. But intake of dioxins and related compounds through fishes and shellfishes was below 5 pg/kg/d. Further monitoring is indispensable.

Animals↗

PubMed1991

Carcinogen adducts as an indicator for the public health risks of consuming carcinogen-exposed fish and shellfish.

A large variety of environmental carcinogens are metabolically activated to electrophilic metabolites that can bind to nucleic acids and protein, forming covalent adducts. The formation of DNA-carcinogen adducts is thought to be a necessary step in the action of most carcinogens. Recently, a variety of new fluorescence, immunochemical, and radioactive-postlabeling procedures have been developed that allow the sensitive measurement of DNA-carcinogen adducts in organisms exposed to environmental carcinogens. In some cases, similar procedures have been developed for protein-carcinogen adducts. In an organism with active metabolic systems for a given carcinogen, adducts are generally much longer lived than the carcinogens that formed them. Thus, the detection of DNA- or protein-carcinogen adducts in aquatic foodstuffs can act as an indicator of prior carcinogen exposure. The presence of DNA adducts would, in addition, suggest a mutagenic/carcinogenic risk to the aquatic organism itself. Vertebrate fish are characterized by high levels of carcinogen metabolism, low body burdens of carcinogen, the formation of carcinogen-macromolecule adducts, and the occurrence of pollution-related tumors. Shellfish, on the other hand, have low levels of carcinogen metabolism, high body burdens of carcinogen, and have little or no evidence of carcinogen-macromolecule adducts or tumors. The consumption of carcinogen adducts in aquatic foodstuffs is unlikely to represent a human health hazard. There are no metabolic pathways by which protein-carcinogen or DNA-carcinogen adducts could reform carcinogens. Incorporation via salvage pathways of preformed nucleoside-carcinogen adducts from foodstuffs into newly synthesized human DNA is theoretically possible.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1993

Simultaneous determination of organotin compounds in fish and shellfish by gas chromatography with a flame photometric detector.

In the present study the levels of the organotin compounds were determined in fish and shellfish which were purchased from retail markets in Niigata, Japan. The concentrations of dibutyltin (DBT), tributyltin (TBT), diphenyltin (DPT) and triphenyltin (TPT) compounds were separately determined by FPD-gas chromatography. Eight species (yellowtail, tuna, cuttlefish, olive flounder, northern shrimp, cinnamon flounder, Japanese sea bass and oyster) were studied and five samples of each species were analyzed in each season, which meant 40 samples in one season, and 160 in total. Among the compounds of interest, DBT, TBT and TPT were detected. The highest concentrations detected were 0.674 microgram/g for DBT, 0.669 microgram/g for TBT and 0.186 microgram/g for TPT. DBT was detected as much as TBT, however, its concentration was not correlated with that of TBT. Seasonal changes of the mean value of the concentration of DBT and TBT were observed. In some species, such as yellowtail and Japanese sea bass, combined contaminations of these organotin compounds were also evident. This study is the first step to elucidate possible health hazards by environmental pollution of organotin compounds on human beings.

Animals↗

PubMed1991

Cholesterol-lowering effect of Agemaki, a kind of shellfish, in mice.

Agemaki (Sinonovacula constricta) is an edible and popular shellfish in the western part of Japan. The present study demonstrated the effects of feeding Agemaki on cholesterol and triglyceride concentrations in mice plasma and liver. Mice were fed a diet containing 0.1% cholesterol and 0.1% Na-cholate for 1 week, and then a cholesterol-free diet or a cholesterol-enriched one for 2 weeks. To both diets, freeze-dried Agemaki was added at a 5% level. There was no statistically significant effect on the body-weight gain, food intake, and liver weight by feeding Agemaki in both dietary regimens. However, Agemaki significantly lowered the concentrations of plasma and liver cholesterol and also of plasma triglyceride in mice feeding on the cholesterol-rich diet. A similar tendency was also observed for the mice feeding on the cholesterol-free diet. The analysis of freeze-dried Agemaki revealed a relatively larger proportion of n-3 polyunsaturated fatty acids and plant sterols, which may possibly decrease plasma lipids. So far as we know, this is the first report showing hypolipidemic effect of Agemaki.

Animals↗

PubMed1998

The incidence of Listeria spp., Salmonella spp., and Clostridium botulinum in smoked fish and shellfish.

The frequency of occurrence of Listeria spp., Salmonella spp., and Clostridium botulinum is samples of smoked finfish and smoked shellfish was analyzed over a 5-year period. Listeria monocytogenes were isolated from 14% of 1,080 samples. For those samples where the smoke process was known, the incidence of L. monocytogenes was higher in cold-smoked than hot-smoked products (51 of 240 cold-smoked compared to 19 of 215 hot-smoked products). Listeria species other than L. monocytogenes were also detected (in 7.2% of cold-smoked and 3.8% of hot-smoked products). The time and temperature smoke processing guidelines are reviewed for a few state authorities. L. monocytogenes was isolated from 15.2% of the 559 samples of foreign origin. There were four countries for which more than 70 samples were analyzed: Canada, Norway, the Philippines, and the United Kingdom. The occurrence of L. monocytogenes in samples from these four countries was 14.3%, 23.7%, 0%, and 16.1%, respectively. The 521 samples originating in the United States were processed by 194 plants. Thirty-seven plants in 13 states produced contaminated product. Salmonella species were isolated from 5 (3.2%) of 156 samples tested for this organism. All positive samples were of foreign origin (4 from the Philippines and 1 from the United Kingdom). No C. botulinum spores were detected in any of the 201 vacuum-packed samples tested for this organism.

Animals↗

PubMed1976

A simplified method for the gas-liquid chromatographic determination of methyl mercury in fish and shellfish.

A simple acetone wash of the fish sample which removes lipids and other organic materials replaces the cystein cleanup specified in other methods. Methyl mercury is freed by hydrochloric acid, extracted into benzene, and determined with a gas-liquid chromatograph equipped with an electron capture detector. The method is quantitative for methyl mercury levels as low as 0.10 ppm in fish and shellfish. Ethyl mercury chloride may be used as an internal standard to detect unsuspected error or instrumental parameter variation.

Animals↗

PubMedDate not supplied

A study of ten toxins associated with paralytic shellfish poison using prechromatographic oxidation and liquid chromatography with fluorescence detection.

Ten paralytic shellfish toxins [saxitoxin, neosaxitoxin, B-1, B-2, gonyautoxin 1, 2, and 3 (i.e., GTX-1, GTX-2, and GTX-3), C-1, C-2, and C-3] were oxidized at room temperature under mildly basic conditions with hydrogen peroxide or periodic acid. The products were then analyzed by liquid chromatography (LC). The N-1-hydroxylated toxins (neosaxitoxin, B-2, GTX-1, and C-3) formed fluorescent products after periodate oxidation at ca pH 8.7, but did not form fluorescent derivatives with peroxide oxidation. The non-N-1-hydroxylated toxins (saxitoxin, B-1, GTX-2, GTX-3, C-1, and C-2) formed highly fluorescent derivatives with both peroxide and periodate oxidations. Individual toxins produced mainly single fluorescent peaks by reverse-phase LC. However, all GTX toxins eluted with the same retention time. Also, C-1 and C-2 eluted together, as did neosaxitoxin and B-2. The non-N-1-hydroxylated toxins could be detected in quantities as low as 20-50 pg/injection, while the N-1-hydroxy analogues could be detected at levels as low as 100-500 pg/injection. UV absorption and fluorescence emission spectra were similar for the oxidation products of all toxins examined (max. 333 +/- 2 nm absorption, 389 +/- 4 nm fluorescence emission).

Animals↗

PubMed1991

Occurrence of faecal bacteria, Salmonella and antigens associated with hepatitis A virus in shellfish.

An investigation was carried out over a one year period to examine jointly the occurrence of faecal bacteria, salmonella and the presence of antigens associated with the hepatitis A virus (HAV) in oysters (Crassostrea gigas), mussels (Mytilus edulis, Mytilus galloprovincialis) and cockles (Cerastoderma edule), taken from 8 shellfish farming areas or natural beds along the French coast. For the faecal coliforms (FC) and faecal streptococci (FS), statistical analysis of the 176 samples examined shows a statistically significant difference between sampling stations (F = 44.39 and F = 26.69 respectively, p less than 0.001): 4 of the 8 stations are more highly contaminated. Salmonella and antigens associated with HAV were detected in 5% and 1.7% respectively of the samples analysed. Frequency of isolation of salmonella is higher for the group of sampling stations where the mean levels of contamination by FC and FS are highest. The presence of HAV associated antigens was detected for the group of stations showing the lowest mean contamination levels. Taking all sample stations together, the percentage of isolation of salmonella differs significantly (chi 2 = 7.28, p less than 0.01) for the two classes of FC established on the basis of the threshold value (300 FC). There is no difference between the two classes of FS. For the HAV-associated antigens, detection percentages are similar for the two classes of results for FC and FS. Within each sampling station, considered independently, no particular correlation was found between the various viral and bacterial markers investigated.

Animals↗

PubMed1991

[11 cases of anaphylaxis caused by grand keyhole limpet (abalone like shellfish)].

We reported 11 cases of patients who developed moderate to severe anaphylactic reactions induced by the ingestion of grand keyhole limpet (GKL) and abalone. Specific IgE-mediated hypersensitivity to these shellfish was demonstrated by history, skin prick test, RAST and immunoblotting. The RAST inhibition technique revealed cross antigenicity between GKL, abalone and keyhole limpet hemocyanin. By immunoblotting analysis, the major antigens of GKL are shown in the MW range of about 38 Kd and 80 Kd.

Adolescent↗

PubMedDate not supplied

Comparison of microwave digestion with conventional wet ashing and dry ashing digestion for analysis of lead, cadmium, chromium, copper, and zinc in shellfish by flame atomic absorption spectroscopy.

A closed vessel microwave digestion procedure was developed for shellfish samples. This procedure was compared with wet and dry ash procedures for levels of lead, cadmium, chromium, copper, and zinc. Results obtained for microwave and conventional wet ash digestion were comparable. The dry ashing procedure produced results consistently lower than either of the other methods. Recoveries ranged from 80-92% for microwave and wet ashing procedures and 54-72% for the dry ashing procedure. Accuracy was also determined by analyzing lobster hepatopancreas marine reference material. Values for Pb, Cd, and Cr fell within the range specified for the reference material for all 3 digestion procedures; however, values were lower for Cu and Zn. Results of this study show that microwave digestion is comparable to wet ashing.

Animals↗

PubMedDate not supplied

Identification of Escherichia coli from shellfish and related environments by automicrobic system.

A total of 463 fecal coliform positive isolates obtained from shellfish and related samples gave typical Escherichia coli IMViC reactions. E. coli identifications for 458 (99%) of these isolates were confirmed using a combination of the Automicrobic System (AMS) and the API 20E system (reference system). The AMS (test system) identified 433 isolates as E. coli; the remaining 25 (5%) isolates were identified as E. hermanii by the test system and as E. coli by the reference system. Additional tests performed on the isolates identified as E. hermanii confirmed those AMS identifications to be incorrect.

Animals↗

PubMedDate not supplied

Rapid determination of methyl mercury in fish and shellfish: collaborative study.

A modification of the official AOAC method for determining methyl mercury in fish and shellfish was studied in 8 laboratories. Methyl mercury is isolated from homogenized, acetone-washed tissue by adding HCl and extracting into toluene the methyl mercuric chloride produced. The extract is analyzed for methyl mercuric chloride by electron capture gas chromatography. Collaborators determined methyl mercury in blind duplicate homogenates at 2 levels in tuna and at 1 level in swordfish and oysters. Collaborators also analyzed single homogenates of swordfish and oysters containing methyl mercury at a second level. Both fortified and unfortified tissues were analyzed. Methyl-bound mercury in the commodities ranged from 0.50 to 2.30 micrograms Hg/g. Reproducibility coefficients of variation ranged from 4 to 15%. Accuracy, measured by comparison to reference values, ranged from 92 to 101%. Recovery from fortified homogenates ranged from 86 to 98%. Reference values and unfortified levels were determined in the author's laboratory by replicate analysis of fortified and unfortified commodities. The method has been approved interim official first action.

Animals↗

PubMedDate not supplied

Rapid, semimicro method for determination of polycyclic aromatic hydrocarbons in shellfish by automated gel permeation/liquid chromatography.

A simple, rapid, easily automated method is described for the determination of polycyclic aromatic hydrocarbons (PAHs) in shellfish such as American lobster (Homarus americanus) and blue mussel (Mytilus edulis). PAHs are extracted from small amounts (1-8 g) of tissue by saponification in 1N ethanolic potassium hydroxide followed by partitioning into 2,2,4-trimethylpentane. This solution is evaporated just to dryness by rotary evaporation and the residue is dissolved in cyclohexane-dichloromethane (1 + 1) for gel permeation chromatography (GPC) on Bio-Beads SX-3. The GPC procedure is ideal as a screening method in the range 25-18 000 ng PAHs/g tissue. If individual PAH measurements are required, the appropriate GPC fraction is collected and PAHs are separated by reverse phase liquid chromatography (LC) with fluorometric detection. Individual PAHs at concentrations as low as 0.25-10 ng/g can be determined. Recoveries of added fluoranthene, pyrene, benz[a]anthracene, chrysene, benzo[e]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenz[a,h]anthracene, benzo[ghi]perylene, and indeno[1,2,3-cd]pyrene were quantitative, with relative standard deviations ranging from 0.0 to 16.9%.

Animals↗

PubMed1977

Survey of finfish and shellfish for volatile N-nitrosamines.

A variety of finfish and shellfish obtained on the local retail market were analyzed for 14 volatile N-nitrosamines by using a gas-liquid chromatographic method demonstrated to be sensitive to 10 ppb (microng/kg). A total of 78 samples (fresh, frozen, and canned), representing 26 varieties of fish, were analyzed. No nitrosamines were found.

Animals↗

PubMed1979

Paralytic shellfish poisoning. A report of 17 cases in Cape Town.

An outbreak of 17 cases of paralytic shellfish poisoning in humans occurred in Cape Town during May 1978. The clinical features were typical and no deaths occurred. Efforts to correlate the severity of disease with the amount of toxin ingested, to demonstrate a protective effect of alcohol, and to demonstrate the immunogenicity of the toxin proved unsuccessful. The regional ecological effects are described. Continued monitoring for the presence of toxic dinoflagellates must be conducted, and the dangers of the consumption of mussels from the Cape west coast should be widely publicized.

Bivalvia↗

PubMed1983

Electron capture gas-liquid chromatographic determination of methyl mercury in fish and shellfish: collaborative study.

A method for determining methyl mercury in fish and shellfish was collaboratively studied in 8 laboratories. Methyl mercury is isolated from acetone-washed, homogenized tissue by adding hydrochloric acid and extracting into benzene the methyl mercuric chloride that is formed. The benzene extract is concentrated and analyzed for methyl mercuric chloride by electron capture gas-liquid chromatography on 5% DEGS-PS treated with inorganic mercuric chloride solution. The quantitation limit for the method is 0.05 micrograms Hg/g. Each collaborator determined methyl mercury at 2 levels in blind duplicate samples of swordfish, tuna, oyster, and shrimp tissues. Both fortified and unfortified samples were analyzed. Methyl-bound mercury in the samples ranged from 0.15 to 2.48 micrograms Hg/g. The reproducibility coefficients of variation for the 8 samples ranged from 3 to 13%. The accuracy, measured by comparison to reference values, ranged from 99 to 120%. Reference values were determined in the Associate Referee's laboratory by replicate analyses of the fortified and unfortified samples. The method has been adopted official first action.

Animals↗

PubMed1981

Diseases from fish and shellfish ingestion.

Fish- and shellfish-acquired diseases are a common but often unrecognized group of food-borne illnesses with considerable epidemiologic importance. They present with a spectrum of gastrointestinal and neurologic symptoms, but the history of pertinent ingestion establishes the diagnosis. Treatment is generally supportive, and recovery with minimal or no residua is the rule. State and local health authorities should be notified of all suspected cases because of the frequent occurrence of multiple cases from one source.

Adult↗

PubMed1996

[Annual daily intakes of Hg, PCB and arsenic from fish and shellfish and comparative survey of their residue levels in fish by body weight].

We have been surveying toxic substances in food and foodstuffs and carrying out a total diet study on the intakes of various substances since 1979 in cooperation with local public institutes in Japan. In this paper, we report the daily intakes of mercury, PCB and arsenic from foods, and the relation between the concentrations of these substance in fish and the fish body weight. The intakes of mercury and arsenic were 6.9-11.0 micrograms/ man/day and 120-230 micrograms/man/day, respectively. The intakes of these substances remained on a stable level from 1979 to 1994. On the other hand, the intake of PCB decreased from 3.1 micrograms/man/day in 1979 to 0.9 microgram/man/day in 1994. Most of the intakes of mercury, PCB and arsenic were derived from the diet group "fish and shellfish". The level of mercury in fish increased with increasing fish body weight. For PCB and arsenic, there was no correlation between these concentrations in fish and the fish body weight, except that mackerel and croaker show a higher concentration of PCB when they are small. Arsenic shows almost a constant level in each fish regardless of their body weight.

Animals↗