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At least 235 records · Page 13Linked to original sources
PubMed2005

A comparative study of adult mosquito trapping using dry ice and yeast generated carbon dioxide.

Adult mosquito collections were conducted for 12 weeks in two residential areas in Kuala Lumpur. The CDC light traps were compared using dry ice and yeast as sources of carbon dioxide attractants for mosquitoes. The efficacy of the dry ice baited trap was significant over yeast generated CO2 trap. The predominant species obtained were Culex quinquefasciatus, Stegomyia albopicta and Armigeres subalbatus.

Aedes↗
PubMed1993

Enzymatic oxidation of cephalosporin C using whole cells of the yeast Triginopsis variabilis within a "cross-flow filter-reactor".

An economical process for the enzymatic oxidation of cephalosporin C to glutaryl-7-ACA was developed at a pilot plant scale. The process utilized nonviable whole cells of the yeast Triginopsis variabilis containing high levels of D-amino acid oxidase. Prior to use, the whole cells were permeabilized with a 25% acetone/water solution which enhanced their apparent activity by 20- to 50-fold. After permeabilization, the whole cells were incubated at pH 11, which served to selectively deactivate catalase which was present in very large quantities. Deactivation of catalase was critical to achieving high reaction yields. The whole cells were utilized within a "cross-flow filter-reactor" which allowed easy and economical recycle of the cells for repeated use. The overall yield of glutaryl-7-ACA from cephalosporin C was 90-95%. The overall productivity of the yeast was 13 kg cephalosporin C oxidized per kilogram yeast (dry basis). The reaction was run at a concentration of 40 g cephalosporin CL-1 and the overall reactor productivity was 11 g glutaryl-7-ACA l-1 h-1. The process has been thoroughly demonstrated on a 35-l scale, and it should be directly scaleable to 10,000 l or more.

Acetone↗
PubMed2003

Development of internal transcribed spacer regions amplification restriction fragment length polymorphism method and its application in monitoring the population of Zygosaccharomyces rouxii M2 in miso fermentation.

Recently, the use of the dry yeast of Zygosaccharomyces rouxii M2 for miso (soybean paste) fermentation has been established. A molecular monitoring method was developed and validated in this study to analyze the population of Z. rouxii M2 during the fermentation. The method was based on the restriction patterns of internal transcribed spacer (ITS) regions of the rDNA using HaeIII and HhaI. Among the homologous ITS regions of Z. rouxii strains, Z. rouxii M2 produced diagnostic bands by which it can be differentiated from the other strains used. The specific restriction bands were due to the difference in nucleotide sequence of two different copies of ITS of Z. rouxii M2. Both ITS copies showed 94% sequence similarity but a 13-bp nucleotide substitution and a 19-bp deletion were found in the ITS1 region. Phylogenetic trees were constructed based on ITS and 18S rDNA sequences and it was found that the ITS sequences provide better resolution for the classification of Z. rouxii M2. Since Z. rouxii M2 is a promising strain for use in miso fermentation as a dry starter, the method developed is significant in terms of industrial application in monitoring the growth of Z. rouxii M2 in miso fermentation.

Journal Article↗
PubMed1990

[Use of fermenter-dialysis culture for the cultivation of medically relevant microbes. III. Candida albicans].

For mass cultivation of Candida albicans (serotype A), a fermenter dialysis culture technique is described and compared with shaking culture and fermenter batch culture techniques. Important growth parameters such as yeast dry weight and viable cell counts demonstrate the advantage of the fermenter dialysis culture. Mannan, the major antigen from Candida albicans prepared by phenol-water extraction followed by gel chromatography was tested with the monoclonal IgM antibody H5.

Candida albicans↗
PubMed1981

[Comparative physiological studies of Candida spec. H and its mutant H 13 during nitrogen starvation].

With Candida spec. H and the mutant H13 during nitrogen starvation we studied the changes occurring in the macromolecular cell composition. In the absence of nitrogen, but in the presence of n-alkanes as carbon source, both the nucleic acid and protein levels of the dried yeasts rapidly decreased. At the same time carbohydrates and lipids were accumulated. Candida spec. H predominantly increased carbohydrate content, whereas the mutant accumulated significantly higher amounts of lipids. The main components of the cellular carbohydrates and the fatty acid content were analyzed, and the results were discussed.

Candida↗
PubMed2004

The interaction of endosulfan with the Collembolan, Proisotoma minuta (Tullberg): toxicity, the effects of sub-lethal concentrations and metabolism.

An increased level of the Collembolan, Proisotoma minuta Tullberg was found in the cotton-growing areas of New South Wales, Australia. They were regularly exposed to endosulfan, a highly insecticidal compound. Toxicity, sub-lethal effects and metabolism of endosulfan were studied in P minuta that had been successfully maintained in a plaster of Paris and charcoal mixture substrate with freeze-dried yeast as a food. The 168-h LD50 values were 0.011, 0.049 and 0.055 mg liter(-1) for alpha- and beta-endosulfan and endosulfan sulfate, respectively. When P minuta were exposed to non-lethal concentrations of the endosulfans, egg production was inhibited. On recovery from the toxic effects of the endosulfans they resumed production of viable eggs. Conversion of alpha- and beta-endosulfan to endosulfan sulfate was found and the endosulfan sulfate could be further metabolized. The rate of metabolism of alpha-endosulfan was greater than that of beta-endosulfan and the product of endosulfan sulfate metabolism was not identified.

Animals↗