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The ependyma of goat. II. Ependymal lining of the lateral ventricles, mesencephalic canal, and fourth ventricle: scanning electron microscopic study.

The ependymal surface of the lateral cerebral ventricle, mesencephalic canal, and IVth ventricle of 13 male and female goats were studied by means of scanning electron microscopy. The lateral cerebral ventricle was covered mostly with thin cilia that might have club-like terminations on nucleus caudatus, the wide "naked" places with bleb-like protrusions occurred in the transition to cornu temporale during anestrous cycle. The ependymal surface of mesencephalic canal of female goats during anestrus was covered with abundant spherical protrusions. No other changes that could be related to the sexual differences were found.

Animals↗

[A scanning electron microscopy study of the ependyma in the lateral cerebral ventricle in humans].

The apical surface of ependymal cells in the cornu anterius of the lateral ventricle of the brain was studied in man. The surface of ependymal cells displays varied differentiation, the cells have microvilli, spherical processes, and cilia. In the cornu anterius ependymal cells without cilia as well as with one cilium and even several cilia were observed. Different types of cells occur in groups forming so-called ependymal areas, which are constituents of the lining of the ventricular walls. Ependymal areas with a large number of cilia were recorded along with areas without cilia and so-called transitory ependymal areas with a small number of cilia. There are no sharp borderlines between the individual areas.

Aged↗

[Ependyma in sheep. 5. Ependymal lining of the cerebral ventricles using scanning electron microscopy].

In 10 ewes and 10 rams the ependymal surface of the brain ventricular system was examined by the scanning electron microscope. The surfaces of the ependymal cells in the upper third of the suprachiasmatic and infundibular regions of the III-rd, partly in the lateral ventricles are covered with dense cilia. A great part of the ventricular surface is covered with microvilli and thin cilia, which are formed into bundles. At certain sites e.g. on the cornu ammonis, suprachiasmatic and infundibular recesses, on the bottom of the aquaeductus mesencephali small foci without microvilli and cilia ("bare") can be observed. In the upper half of the suprachiasmatic region some of the cilia have button-like and in the cornu temporale in the lateral ventricle club-like terminations. The building of the ependymal surface in the infundibular recess in sheep depends on the sex; in the ewes it altered in correlation to the stage of the ovarian cycle and in the rams according to the season. Supraependymal cells were found only in ewes during estrus, in rams they were not found. According to the external morphology the supraependymal cells are divided into three groups.

Animals↗

Ependyma of the central canal of the rat spinal cord: a light and transmission electron microscopic study.

Ependymal cells of the rat central canal were examined with a view to identifying features that distinguish them regionally and from their counterparts elsewhere in the ventricular system. The results revealed that the lining consisted for the most part of a pseudostratified layer of uniformly organised cuboidal to columnar ependymal cells present in largest numbers in lumbar and sacral segments and in the conus. Two cell variants were identified on the basis of the presence or absence of a radially directed cytoplasmic process originating from the base of the cell. The tanycytic form of ependymal cell was encountered along the entire length of the central canal but with increased frequency in caudalmost segments. Ependymal cells were largely similar in ultrastructural appearance along the length of the cord. Although they were also similar in appearance and orientation to their counterparts in the ventricles they did exhibit some unique features. The most notable were the prominent junctional complexes and associated filaments present along the lateral border of the cells near their apex and the abundance of intermediate filaments in tanycytes. The central canal of the filum differed most markedly from other segments of the cord and resembled in structure the primitive ependymal tube of the caudal cord in lower vertebrates. Ependymal cells of the cord were not sufficiently dissimilar morphologically from their counterparts in the ventricles to account for differences in proliferative capacity in response to localised injury. A factor that merits further study is the difference in numbers of tanycyte ependymal cells in the two locations for they may be the reactive elements that proliferate in response to injury.

Animals↗

Modifications in cortical ependyma of the lizard, Podarcis hispanica, during postnatal development.

The ependymal surface, cell number, cell size and cell density were measured in adult and neonatal exemplars of the lizard, Podarcis hispanica. The ependymal monostratified surface increases with age, while that of sulcal areas decreases and the ependymocyte number remains constant. The mitotic activity found in the neonatal sulcal areas could explain the postnatal increase of neuronal number described for the cerebral cortex of the studied species.

Animals↗