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PubMed1985

Microorganisms in semen used for artificial insemination.

The effect of freezing semen in a cryopreservative media consisting of egg yolk glycerol with or without erythromycin was tested for its effect on the viability of microorganisms present in donor semen and on sexually transmitted pathogens seeded into semen. All donor semen contained two or three species of microorganisms that could be considered skin flora. Five of ten donor semen specimens contained Ureaplasma urealyticum that was not affected by either freezing or antimicrobial treatment. Some strains of Neisseria gonorrhoeae, when seeded into semen, survived all conditions except freezing in egg yolk glycerol containing erythromycin. Chlamydia trachomatis was erradicated when erythromycin was present in the cryopreservative. There was no detectable effect of any treatment tested on the survival of herpes simplex virus.

Adult↗
PubMed1986

Pregnancy rates with artificial insemination by donor: the influence of the cryopreservation method and coexistent infertility factors.

Women entering the AID programme in Dunedin since 1983 were studied to assess the clinical performance of cryopreservation modifications as well as the early assessment of coexistent infertility factors. The cumulative pregnancy rate for normal pregnancies was 45.5% and 64.7% for three months and six months respectively. The conception rate per cycle was 17.9%. These compare favourably with the use of fresh semen in other reports. Except for endometriosis and possibly tubal polyps, treatment of coexistent infertility factors retained this conception rate. With refinements in techniques it is possible that conception rates with AID could approach those of normal fertility.

Adult↗