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PubMed1977

[Studies on the tolerance of the organism to X 5 CrNiMo 18.10 steel (Königsee). I. Detection of chrome and nickel traces in the spleen, liver, kidney and in the tissues surrounding metallic implants (X 5 CrNiMo 18.10 steel) in guinea pigs by means of flameless atomic absorption spectrophotometry].

Small bars of X 5 CrNiMo 18.10-steel were implanted under the dorsal muscles of 60 guinea-pigs, a number of which was preoperatively sensibilized against potassium-bichromate or nickel-II-sulfate. After various times of surviving the traces of chromium and nickel were assessed in the tissue around the graft, in spleen, liver, and kidney. The resulting values ranged widely, there was a tendency to increased values the longer the implantates remained in the tissue. Traces of the elements were also found in the organs of the guinea-pigs. After sensibilization, the animals showed no more aggressive behavior against the implantates than without sensibilization. The quality of the implantates' surface is more important - as to coming-off parts of the alloy by corroding - than the preoperative immune condition of the organism.

Biocompatible Materials↗
PubMed1981

Determination of platinum in serum and ultrafiltrate by flameless atomic absorption spectrophotometry.

A graphite furnace atomic absorption spectrophotometric assay, capable of accurately determining nanogram amounts of platinum in serum and ultrafiltrate, was developed. A sample serum or ultrafiltrate was acidified with nitric acid and heated to destroy the protein-platinum bond. A measured excess of ammonium 1-pyrrolidinedithiocarbamate was added, and the platinum complex was extracted into isopropylacetone. The extract was injected into the graphite furnace. The sample was dried, charred, and atomized using optimal conditions. The resulting absorbance was used to determine the platinum content.

Humans↗
PubMed1990

Determination of lithium in rat brain regions and synaptosomes by graphite furnace atomic absorption spectrophotometry.

A graphite furnace atomic absorption spectrophotometric method for the analysis of very low concentrations of lithium in brain tissue and subcellular fractions is described. The method has a picogram sensitivity and shows a precision value of 10.1% expressed as the per cent variation coefficient for the tissue analysis of the metal. Lithium concentration in eight regions of the rat brain and regional synaptosomal lithium contents were analyzed with the method described. Results from these determinations show that lithium is heterogeneously distributed among rat brain regions 24 hr after a single s.c. lithium administration; hypothalamus, corpus striatum and midbrain were the regions with the highest lithium accumulation. Lithium is homogeneously concentrated in the synaptosomes obtained from rat brain regions. The method proposed may be considered adequate for trace lithium analysis in pharmacological studies of the metal.

Animals↗
PubMed1975

Potential sources of copper contamination of farm milk supplies measured by atomic absorption spectrophotometry.

A method was developed to use atomic absorption spectrophotometry for copper measurements in milk filtrates prepared from equal volumes of milk and 20% trichloracetic acid. Results are reported for 11 farms for water supplies, wash and rinse solutions, and milk. Copper below 10 mug/liter was found at the well for the 5 farms with their own spring supply. Averages and ranges for all 11 farms in mug/liter were 33 (0 to 156) at the cold water tap in the milk house, 220 (8 to 1,360) at the hot water tap, 246 (8 to 1,722) in the wash sink with added detergent and hot water, and 275 (9 to 1,823) after using the wash solution. Rinse solution with hypochlorite in cold water showed a rise from 31 (1 to 157) before use to 51 (5 to 206) after use. Copper in milk averaged 99 mug/liter (72 to 163). In spite of relatively high copper in wash solutions, copper in milk was not correlated postively, giving rise to question whether adsorbed copper from such solutions can contribute much copper to milk. Thorough rinsing with low copper cold water and a high dilution ratio in milk were seen as mitigating factors. Direct contact of milk with copper bearing metals presented a greater opportunity for contamination. Removal of substantial amounts of copper from pipe lines and bulk tanks with sulfamic acid was demonstrated.

Agriculture↗
PubMed1992

Determination of aluminum in biological fluids by furnace atomic absorption spectrophotometry.

Detailed procedures were developed for the furnace atomic absorption spectrophotometry (FAAS) determination of aluminum (Al) in serum, urine, cerebrospinal fluid (CSF), and proportionated dialysate. Of particular note were the use of Mg (NO3)2.6H2O as a matrix modifier and the employment of the standard additions routine in analysis. The accuracy of the method(s) used is supported by work with assayed controls and by recovery studies. The use of a "clean room" was shown to be unnecessary. Normal serum, urine, and CSF Al ranges observed were 4.8-8.9, 5.1-9.1, and 1.0-5.8 micrograms L-1 respectively.

Aluminum↗
PubMed2000

[Determination of eight trace elements in kadsura coccinea by flame atomic absorption spectrophotometry].

In this paper, we used the flame atomic absorption spectrophotometry to determine the contents of Zn, Fe, Ca, Cu, K, Na, Mn, Mg in the kadsura coccinea. We assayed the determination conditions systematically. The SrCl2 was sued to eliminate coexisting elements. And we achieved the purpose that we can determine a lot of elements in the same liquor at the same time. It shows that this method is simple, convenient and accurate.

Calcium↗
PubMed1976

[Determination of the concentration of copper, lead, cadmium and zinc in milk by atomic absorption spectrophotometry].

Developed was an atom-absorption method for determining microconcentrations of copper, lead, cadmium, and zink in milk originating from various regions of this country. The study was carried out by means of an atom-absorption spectrophotometer AA-5. Established were the optimal conditions for determining the trace elements reaching the following values of susceptibility: for copper--0.04; for lead--0.11; for cadmium--0.001; and for zinc--0.009 mg/cu.cm, with an allowance of 5%. The results obtained are discussed and statistically processed. Literature data are comparatively studied reflecting the amounts of these elements in milk as found in this country and abroad. Such data will be made use of in the elaboration of Bulgarian reference tables necessary for establishing various regimes and norms concerning the food products offered in children creches, hospitals, canteens, etc.

Animals↗
PubMed1976

Determination of vitamin B12 in dry feeds by atomic absorption spectrophotometry.

Vitamin B12 was determined in dry feeds by atomic absorption spectrophotometry (AAS). Samples containing B12 were extracted with an assay solution, 5 g EDTA was added to the filtrate, the pH was adjusted to 7 with NH4OH, and 5 g charcoal was added. The charcoal was removed by filtering through ashless paper which was then placed in a beaker and ashed at 600 degrees C. After dissolving the cobalt oxide from the ash in 5N HNO3, cobalt content was determined by using AAS. To determine mg B12/lb feed, ppm cobalt in the feed is multiplied by 10.43. The sensitivity of the proposed procedure is 1 mg vitamin B12/lb. The procedure is rapid and precise, and results compare favorably with AOAC method 43.109.

Animal Feed↗
PubMed1980

Electrothermal atomic absorption spectrophotometry of cadmium in semen.

We describe the electrothermal atomic absorption spectrophotometry of cadmium in rabbit semen collected before and after seven days of subcutaneous administration of 0.5 mg of cadmium per kilogram body weight per day. The analytical technique involves combining an aliquot of an acid-digested semen sample with an equal volume of an (NH4)2HOP4 solution (50 g/L), to allow an increase in charring temperature, which results in more nearly complete destruction of matrix. Cadmium values as determined by this method correlated well with those determined by the method of standard additions. The dosing regimen resulted in a statistically significant (p < 0.01) increase in cadmium concentrations in semen (and whole blood) sampled just after the last day of cadmium administration.

Animals↗
PubMedDate not supplied

Comparative sensitivities of flame atomic absorption spectrophotometry (AAS) and molecular absorption spectrophotometry (MAS).

A sensitivity comparison was made between conventional atomic absorption spectrophotometry (AAS) and conventional molecular absorption spectrophotometry (MAS) for the serum trace metals, copper, iron, and zinc. The sensitivity aspect considered was absorbances was obtained for concentrations in the solutions analyzed using unit lightpaths of 1 cm and 10 cm for MAS and AAS, respectively. A distinction was made between procedural sensitivity and measurement sensitivity where the latter represents molar absorptivity. Some was given to the concepts of procedural sensitization by concentration of the analyte through the use of lyophilization, extraction or ashing. The misuse of data obtained with scale expanders is described as a commonly occurring phenomenon of both AAS and MAS and a source of confusion in the understanding of measurement sensitivity.

Copper↗