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PubMedDate not supplied

Rapid determination of tannins in tanning baths by adaptation of BSA method.

A rapid and reproducible method for the determination of tannins in vegetable tanning baths is proposed as a modification of the BSA method for grain tannins existing in literature. The protein BSA was used instead of leather powder employed in the Filter Method, which is adopted in Italy and various others countries of Central Europe. In this rapid method the tannin contents is determined by means a spectrophotometric reading and not by means a gravimetric analysis of the Filter Method. The BSA method, which belongs to mixed methods (which use both precipitation and complexation of tannins), consists of selective precipitation of tannin from a solution containing also non tannins by BSA, the dissolution of precipitate and the quantification of free tannin amount by its complexation with Fe(III) in hydrochloric solutions. The absorbance values, read at 522 nm, have been expressed in terms of tannic acid concentration by using a calibration curve made with standard solutions of tannic acid; these have been correlated with the results obtained by using the Filter Method.

Absorption↗
PubMed2003

Using a vortex mixer for testicular sperm collection.

OBJECTIVE: To evaluate 2 methods of processing testicular tissue for the retrieval of viable sperm from men with nonobstructive azoospermia. STUDY DESIGN: Fresh testicular tissue was obtained from nonobstructive azoospermia patients using a biopsy needle. The specimens were divided into 2 fractions. All specimens were minced and immersed in human tubal fluid (HTF). The first fraction was filtered through a nylon filter and incubated for 3 hours. The supernatant was centrifuged, resuspended in HTF and analyzed. The second fraction was immediately vortexed for 5 minutes and filtered through a nylon filter. The supernatant was centrifuged, resuspended in HTF and analyzed. RESULTS: Spermatozoa were obtained in 13 of 24 cases (54.2%) using the vortex method and in 5 of 24 cases (20.8%) with the nylon filter method. CONCLUSION: The vortex mixing method may be a better option than the conventional method for processing testicular tissue for sperm collection.

Adult↗
PubMed1990

[Methods in mixed ventilation: advantages, disadvantages and consequences for work of breathing].

During partial ventilatory support modes both the ventilator and the patient are performing mechanical work. These modes are currently used as mechanical ventilatory support to provide a better synchronism between the patient and the machine and to avoid a complete rest of the respiratory muscles, or as modes of weaning from mechanical ventilation. Assist control ventilation allows the patient to regulate his own respiratory rate by triggering preset ventilatory cycles. Synchronized intermittent mandatory ventilation includes both controlled cycles and spontaneous breathing cycles. Lastly, during inspiratory pressure support, each spontaneous cycle is assisted by pressurization of the circuit. This latter modality alone seems efficiently to decrease the patients' breathing work while allowing complete synchronism between patient and ventilator.

Humans↗