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[Detection of pathogenic mycobacteria in the environment of the medical units and of the slaughter-house of an African town (author's transl)].

The authors have made investigations about the presence of pathogen mycobacteria in puddles of rain water and in rill waters of sanitary formations and municipal slaughter-house of Yaoundé. 19 strains of pathogen mycobacteria have been isolated from 84 water samples : 15 M. tuberculosis strains, especially present in the environment of sanitary formations, 4 M. bovis strains, especially present in the environment of the slaughterhouse. The third part of isolated M. tuberculosis strains belongs to the africanum variety of this species, although this variety is prevalent in the human pathologic products. 13 from 19 strains are I.N.H. susceptible.

Abattoirs↗

[Detection of pathogenic leptospira in the waste water and sewage sludge of large pig breeding sites].

Sewage effluent and sludge from the purification plant of 8 large piggeries was examined for the presence of pathogenic leptospires. By using the methods of Appelman and Van Thiel it was found that 43.1% of samples of effluent were contaminated with L. pomona and O. tarassovi. Altogether 33 strains of pomona and three mixed cultures of pomona and tarassovi were obtained. The isolated strains were shown to be pathogenic by experimental infection of guinea-pigs, rabbits and pregnant and non-pregnant sows. The average period of survival of pathogenic leptospires in sewage effluent was 24 to 48 hours, with a maximum of 96 hours. Leptospires were killed within 24 hours in decanted sludge, owing to its strong acidity.

Animals↗

Detection of pathogenic Leptospira spp. infections among mammals captured in the Peruvian Amazon basin region.

To identify potential zoonotic reservoirs of pathogenic leptospires in the Peruvian Amazon basin, wild mammals were trapped from July 1997 to December 1998 near the city of Iquitos. After extraction of nucleic acids from animal kidneys, DNA of pathogenic leptospires was identified by polymerase chain reaction (PCR) assays using one of two primer sets, one amplifying a region of the 23S rRNA gene, and the other amplifying a gene fragment specific for Leptospira spp (G1/G2 primers). Overall, 29% (40 of 136) of the mammals tested showed evidence of renal infection by Leptospira spp., including 20% (13 of 64) of the rodents, 39% (20 of 51) of the marsupials, and 35% (7 of 20) of the chiropterans (bats). Marsupials and chiropterans were implicated as more significant reservoir hosts of leptospires pathogenic to humans than previously recognized.

Animals↗

[Detection of pathogenic Enterobacteriaceae in seawater].

As a result of investigations carried out in the littoral sea waters (a total of 114 sea water samples were analyzed) there were isolated 226 strains of salmonellae referred to 9 serological types, this constituting 35% of the positive samples. As revealed, salmonella distribution in sea water depended on the site of outflow of sewage, and also on the unfavourable influence of rivers inflowing to the littoral part of the sea.

Health Resorts↗