[Discussion of methods of measuring basal metabolism].
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To evaluate whether bioimpedance analysis (BIA) is a useful tool for predicting basal metabolic rate (BMR), sex, age, height, weight, BMI, and single-frequency BIA variables (resistance index and phase angle) were assessed in 61 young adult non-diabetic obese patients (BMI >35 kg/m(2)). BMR was measured by indirect calorimetry. In both sexes BMR significantly correlated with weight, BMI, and resistance index. Using multiple regression analysis, the following prediction equations were derived: (1). considering individual characteristics: BMR (kcal/day)=780+11.4xweight (+221 for men); (2). including also BIA variables: BMR=- 96+8.4xresistance index+8.3xweight+82.5xphase angle. Thus, in young adult, severely obese individuals, BIA variables are significant predictors of BMR.
Since the World Health Organization suggested predictive equations for basal metabolic rate (BMR) in 1985 there has been great interest in their validity in different populations worldwide. It has been shown that these equations overestimate BMR in some populations, particularly the ones living in the tropics. There is limited new information on BMR in segments of the Brazilian population. Therefore, the aim of the present study was to compare measured with estimated BMR using some published predictive equations in 50 college students from Rio de Janeiro, Brasil. BMR was measured by indirect calorimetry and the predictive equations used were the ones published by: FAO/WHO/UNU (1985); Harris & Benedict (1919), and Henry & Rees (1991). Estimated BMRs were significantly greater than measured BMR (p < 0.05). Overestimation was greatest with the equation published by Harris & Benedict (18.9%) followed by the ones by FAO/WHO/UNU (12.5%) and Henry & Rees (7.2%). Body composition did not correlate with the overestimation of BMR. More data are necessary so that appropriate predictive equations can be developed for the Brazilian population.
Many birds exhibit considerable phenotypic flexibility in maintenance energy requirements, and up- or downregulate basal metabolic rate (BMR) over time scales of days to weeks during thermal acclimation. However, the extent to which individual birds can reverse the direction of BMR adjustments over short time scales remains unknown. In this study, we examined metabolic responses to short-term thermal acclimation in laughing doves Streptopelia senegalensis. In 30 wild-caught doves (mean body mass=92.6 g) divided into three experimental groups of 10 birds each, initial BMR averaged 0.760+/-0.036 W. Thereafter, each group was acclimated to one of three acclimation air temperatures (T(acc)=10, 22 or 35 degrees C) for 21 days, during which time the doves were housed in individual cages. Following the first acclimation period (acclimation I), BMR (W) was significantly lower and was negatively and linearly related to T(acc) [BMR=0.714-0.005T(acc)]. Acclimation I BMR varied from 0.546+/-0.039 W in doves acclimated to T(acc)=35 degrees C to 0.665+/-0.058 W at T(acc)=10 degrees C. A second acclimation period of a further 21 days (acclimation II) revealed that the direction of BMR adjustments could be reversed within individuals, with acclimation II BMR again negatively and linearly related to T(acc). The slope of the relationship between BMR and T(acc) following acclimation II was not significantly different to that following acclimation I. BMR exhibited consistent inter-individual variation, with a low but significant repeatability of 0.113. The within-individual BMR variation of up to 26% that we observed in laughing doves reveals that BMR is a highly flexible trait in this species, and reiterates the need to take phenotypic plasticity into account in comparative analyses of avian energetic parameters.
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To study whether mounting an immune response is energetically costly, mice from two lines divergently selected for high (H-BMR) and low (L-BMR) basal metabolic rate (BMR) were immunized with sheep red blood cells. Their energy budgets were then additionally burdened by sudden transfer from an ambient temperature of 23 degrees C to 5 degrees C. We found that the immune response of H-BMR mice was lower than that of L-BMR mice. However, the interaction between line affiliation and ambient temperature was not significant and cold exposure did not result in immunosuppression in either line. At 23 degrees C the animals of both lines seemed to cover the costs of immune response by increasing food consumption and digestive efficiency. This was not observed at 5 degrees C, so these costs must have been covered at the expense of other components of the energy budget. Cold exposure itself elicited a considerable increase in food intake and the mass of internal organs, which were also heavier in H-BMR than in L-BMR mice. However, irrespective of the temperature or line affiliation, immunized mice had smaller intestines, while cold-exposed immunized mice had smaller hearts. Furthermore, the observed larger mass of the liver and kidneys in immunized mice of both lines kept at 23 degrees C was not observed at 5 degrees C. Hence, immunization compromised upregulation of the function of metabolically active internal organs, essential for meeting the energetic demands of cold. We conclude that the difficulties with a straightforward demonstration of the energetic costs of immune responses in these animals stem from the extreme flexibility of their energy budgets.
Exposure to organohalogens in endotherms has been suggested to impose chemically induced stress by affecting functions related to maintenance energy requirements. Effects on basal metabolic rate (BMR) have been suggested to be, in part, mediated through interactions with the thyroid hormones (THs). We investigated the relationships between plasma concentrations of major organochlorines, PBDEs, hydroxylated (OH)- and methoxylated (MeO)-PBDEs and OH-PCBs, circulating TH levels and BMR in breeding glaucous gulls (Larus hyperboreus) from the Norwegian Arctic. Negative associations were found between BMR and concentrations of sigma PCB, Sigma DDT and particularly Sigma chlordane, which combined made up 91% of the total contaminant burden. Levels of THs (thyroxine and triiodothyronine) were not associated significantly with variation of BMR or concentrations of any of the compounds determined. The present study suggests that BMR may be altered in glaucous gulls exposed to high loadings of persistent contaminants in the Norwegian Arctic environment.
Undernutrition in rats for 60 days from the first day of birth resulted in a significant diminution of nonshivering thermogenesis (NST) and basal metabolic rate (BMR; 12.5 and 5% respectively) as compared to their controls, even when expressed on a metabolic body weight basis. Sucrose (8%) feeding for 72 h of such undernourished (UN) rats immediately after 60 days reverted the depressed NST capacity to levels comparable to control values while the BMR was higher by about 9 per cent than controls. The actual increase in NST and BMR of UN rats after 72 h of sucrose feeding was about 12 and 14 per cent respectively when compared to the values before sucrose feeding. This is possibly because sucrose feeding is known to elevate the caloric intake by 20-30 per cent over normal and would stimulate the sympathetic nervous system (SNS) activity. The results thus would imply that the reduced NST and BMR in rats consequent to undernutrition is probably due to diminished SNS activity which may be associated with changes in some hormonal and brown adipose tissue functions. However, such changes appear to be limited only to the period of undernutrition as it can be corrected speedily by adequate energy supplementation.
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Man and experimental animals are able to adapt to restricted levels of energy intake mainly by a reduction in basal metabolic rate (BMR) while the effects of energy restriction on the specific dynamic action (SDA) of food and the spontaneous activity are less well established. In this study, growing rats were fed ad libitum or restricted amounts of diet during 28-31 days. Then total heat production (THP) and BMR during 22 hours, as well as SDA of a test meal, were measured on individual rats by indirect calorimetry. After the calorimeter study rats were kept in metabolic cages for 6 days after which they were killed and analyzed for total body composition. The energy balance of the rats over the last 7 days of the study was calculated from changes in body composition and measurements of food intake during this period. During the calorimeter experiment an indirect estimation of the spontaneous activity of the rat was made. Results showed that THP, BMR and SDA of restricted rats were significantly lower than for ad libitum-fed rats. From body composition measurements, rats restricted in food intake before the calorimetry study were found to mobilize more protein and less fat compared to rats fed ad libitum until the calorimetry and body composition studies were carried out. No difference in spontaneous activity was observed between the two dietary treatments.