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PubMed1983

Circulating corticosterone responses of feed and water deprived broilers and Japanese quail.

Experiments were conducted to delineate the acute stress response of commercial broilers to feed and water deprivation for 10 hr. The effect of method of nutrient deprivation, cooping versus removal of feed and water from broiler floor pens, was also considered. Bihourly plasma corticosterone measures were made during 10-hr withdrawal periods, and significant alterations in this adrenal steroid were considered indicative of stress. In all studies, feed and water deprivation produced significant elevations in plasma corticosterone concentrations. Cooped broilers exhibited a shorter onset and more exaggerated magnitude of adrenal responsiveness than floor penned broilers deprived of feed and water. There was also evidence that plasma corticosterone secretion in floor penned broilers was continual (linearly increasing) throughout the 10-hr withdrawal period. In contrast, maximal corticosterone responses followed by waning hormonal levels (an increasing quadratic function) were evident in cooped broilers. It was concluded that procedures involved in physically cooping birds, as well as the restraint cooping imposed, were acting additively to the stress associated solely with feed and water deprivation. Changes in plasma volume, as evidenced by percent packed cell volume changes during treatment periods, were not responsible for the corticosterone elevations observed. In a separate experiment, battery housed Japanese quail deprived of feed and water for 12 hr exhibited a temporal plasma corticosterone response very similar to that observed in floor penned broilers deprived of feed and water. Full-fed control quail exhibited only random temporal hormonal fluctuations during this time. It was likewise concluded that the practice of feed and water deprivation in quail constitutes a significant nonspecific stressor.

Animals↗

PubMed1983

Individual and combined effects of aflatoxin and ochratoxin A on bruising in broiler chickens.

A 2 x 2 factorial design with treatments of 0 and 2.5 microgram/g aflatoxin and 0 and 2.0 microgram/g ochratoxin A was used to evaluate the individual and combined effects of these mycotoxins on bruising and blood thigh syndrome in broiler chickens. Trial 1 consisted of four replicate pens of 10 broilers per replicate, which were maintained on these dietary treatments from 0 to 3 weeks of age. At 3 weeks of age two replicate pens per treatment were randomly selected and placed on toxin-free feed with two replicate pens remaining on toxin feed until they were 6 weeks of age when the trial was concluded. In Trial 2 three replicate pens of 38 broilers per treatment were maintained on toxin feed from 0 to 3 weeks of age. At 3 weeks of age 13 broilers per replicate were placed on toxin-free feed with 25 broilers per replicate remaining on toxin until they reached 7 weeks of age when Trial 2 was concluded. These data indicate that a synergistic toxicity exists between aflatoxin and ochratoxin A to significantly (P less than .05) decrease body weight. Body weights of broilers on aflatoxin or ochratoxin A diets for only 3 weeks partially recovered by 6 or 7 weeks of age; however, the body weights of broilers on the interaction treatments for only 3 weeks remained significantly (P less than .05) depressed at 6 and 7 weeks of age.(ABSTRACT TRUNCATED AT 250 WORDS)

Aflatoxins↗

PubMed1983

Effects of acute ochratoxicosis on blood pressure and heart rate of broiler chickens.

Male broiler chickens (Hubbard X Hubbard) were placed at hatching into electrically heated batteries with feed and water available ad libitum and maintained to 3 weeks of age under continuous lighting. In Trial 1, two replicate pens of 10 broilers per replicate were fed ochratoxin A from day-old to 3 weeks of age with treatments of 0, 2.0, and 4.0 micrograms/g ochratoxin A in feed (ppm). Trial 2 was identical to Trial 1 except that when the broilers reached 3 weeks of age they were administered an additional intravenous dose of either ochratoxin A, methoxamine, or isoproterenol. Trial 3 broiler chickens were maintained on control feed until they reached 3 weeks of age at which time they were taken off of feed for 2.5 hr and then placed on either control feed or feed containing 4.0 ppm ochratoxin A, and heart rate and blood pressure were measured every half hour through 7 hr. In Trial 1, no effect of ochratoxin A was seen in any treatment on heart rate or diastolic, systolic, or mean blood pressure. However, when an intravenous dose of ochratoxin A was administered to these broilers, a significant (P less than .05) decrease in heart rate and diastolic, systolic, and mean blood pressure occurred. The severity and duration of intravenous ochratoxin A administration were significantly (P less than .05) enhanced as the level of dietary ochratoxin A increased. The response of broilers to isoproterenol was significantly (P less than .05) altered by dietary ochratoxin A, whereas the broilers responded in a predictable manner to methoxamine without significant (P less than .05) ochratoxin A treatment effects.(ABSTRACT TRUNCATED AT 250 WORDS)

Administration, Oral↗

PubMed1984

Plasma triglyceride and glucose clearance in broiler-type and white Leghorn chickens with different degrees of adiposity.

Broiler-type and White Leghorn chicks were given diets containing 30.5, 26.2, or 22.3% protein with and without addition of 5% corn oil during an 8- to 9-week conditioning period. The absolute weight of the abdominal fat pad was greater in the broiler-type birds on all dietary treatments. As a percentage of body weight the abdominal fat pads were similar in broiler-type and White Leghorn birds only when the diet contained 30.5% of protein without added oil. Following a 16-hr fast, plasma glucose was higher in the broiler-type than in the White Leghorn birds, whereas plasma triglyceride concentrations were higher in White Leghorns. In birds refed a high-fat meal, plasma glucose showed little change except in the broiler chickens previously fed the 30.5% protein diet without added oil. In these birds plasma glucose declined over a 5-hr period to reach a mean value of 130 mg/100 ml. Plasma triglyceride concentrations in both breeds were markedly elevated within 45 min of refeeding with a high-fat meal, but the peak concentrations were higher in the White Leghorns than in the broiler-type birds. Previous consumption of the high-oil diet increased postprandial triglyceride concentration in the plasma of the broiler-type but reduced it in the White Leghorn chickens. The difference in response of the chickens of the different genetic stocks is attributed to differences in the development of the activities of the systems associated with 1) fatty acid absorption and movement of triglycerides from the intestinal mucosa to the circulation and 2) clearance of plasma triglycerides to the adipocytes as a result of lipoprotein lipase activity.

Adipose Tissue↗

PubMed1984

Drinking water treatment with a commercial preparation of a concentrated Lactobacillus culture for broiler chickens.

Three hundred male broiler chicks were used to determine the effects of supplying Lactobacillus in the drinking water. The product used, Biomax 40TM, was a fresh-frozen, pure culture of Lactobacillus containing 40 X 10(9) cfu/ml. Water treatments consisted of continuous lactobacilli dosing (CD) and skip-a-day lactobacilli dosing (SAD). A control group (C) received no lactobacilli. Each treatment and control contained two pens of 50 chicks each and were fed for a duration of 7 weeks. At the termination of the experiment, weights of the wet viscera, and wet and dry small intestines of the broilers revealed no significant differences between treatments and control. Surface pH readings taken from crop and duodenum showed that there was no significant difference (P greater than .05). Microbiology performed on duodenal contents revealed higher numbers of lactobacilli for CD and SAD broilers than for C broilers. The liver biotin contents of the lactobacilli-treated broilers were not significantly different from those of the controls. Body weights were not changed by either treatment, although they were greater for the CD broilers than for the SAD broilers. No difference was observed in feed conversion (feed/gain).

Animals↗

PubMed1991

The effect of feed intake on body temperature and water consumption of male broilers during heat exposure.

Two trials were conducted to study the effect of feed intake on rectal temperature and water consumption of acclimated and unacclimated broilers during heat exposure. Male broiler chicks, 100 per trial, were raised as one group to 29 days for Trial 1 and 36 days for Trial 2. The birds were moved to environmental chambers and 50 per trial were acclimated by being subjected to 3 consecutive days of 24 C, 35 C, 24 C cyclic temperature. After acclimation, the birds were exposed to a linear change in temperature from 24 to 41 C over 3 h with a constant 10 C dewpoint, starting at 0800 h on Day 34 for Trial 1 and on Day 41 for Trial 2. The feeding schedule for the day of the heat exposure was as follows. For the feed group, feed was removed from the birds at 0400 h and placed back at 0700 h. For the no feed group, feed was removed at 0700 h. At 0800 h, feed was removed from all birds but water was accessible throughout the heat exposure. Acclimated and unacclimated birds given access to feed for 1 h before a heat exposure consumed 60 and 50 mL of water per broiler, respectively, during the heat exposure. However, for broilers not receiving feed, the water consumption was 58 and 30 mL per broiler, respectively. Acclimated and unacclimated broilers given access to feed had similar body temperatures, but the acclimated broilers not receiving feed had a significantly lower ending rectal temperature than the unacclimated group.

Animals↗

PubMed1991

Effects of dietary amino acid levels on performance and carcass composition of broilers 42 to 49 days of age.

Broilers were grown to 42 days on nutritionally complete diets. From 42 to 49 days in two trials, broilers were fed diets formulated to contain 70, 80, 90, 100, 110, or 120% of recommended amino acid (AA) values. In four additional trials, the same AA levels were fed; in addition, Met and Lys were added to diets formulated to contain 70, 80, and 90% to equal levels of the 110% AA diet. Body weight gain (BWG), feed consumption (FC), feed conversion (FCR), dressing percentage (DP), and abdominal fat (AF) were measured. From 42 to 49 days, dietary AA levels had little effect on BWG, DP, or AF of female broilers. When male broilers were fed diets containing less than 100% of the recommended AA levels, BWG was reduced and AF increased. Broiler FC increased and FCR decreased as the AA level of the diet decreased. The addition of Met and Lys to the 90% AA diet improved performance; however, the addition of Met and Lys to the 70% AA diet had no beneficial effect. Amino acid levels may be reduced in broilers diets fed from 42 to 49 days; however, male broilers are more sensitive than females to reductions in AA levels during this period.

Abdomen↗

PubMed1991

Performance evaluation of heat-stressed commercial broilers provided water-cooled floor perches.

A study was conducted to determine whether water-cooled floor perches would be utilized by commercial broilers exposed to a constant hot ambient environment; and subsequently, whether utilization of these perches would improve performance beyond those provided uncooled floor perches. A total of 330 day-old commercial broiler chicks were randomly allocated to six pens (2.44 m2) in an environmentally controlled facility and maintained in a thermoneutral brooding environment for 16 days. Following this period, 240 birds were selected on a body weight basis and randomly assigned to the six pens. A perch constructed from steel pipe (2.44 m length, 5.0 cm diameter) was then placed diagonally on the litter covered floor of each pen. The birds were first exposed to a thermoneutral period (27.7 C), during which time cooling of the perches in three replicate pens was initiated by circulating tap water. The other three experimental pens received ambient perches. Ambient temperature was then raised to 32.6 C for the following 4 wk. The results of the present study showed that utilization of water-cooled perches by broilers was greater (P less than or equal to .01) than ambient perch utilization throughout the 32.6 C period. Average daily gain was greatest (P less than or equal to .01) for broilers exposed to cool perches. Additionally, they consume more feed (P less than or equal to .05), on a daily basis, than those given ambient perches during the heat-stress period. Broilers exposed to water-cooled perches also had a more efficient gain to feed ratio (P less than or equal to .01). At the completion of the study, final body weight and total body weight gain were greater (P less than or equal to .05) for broilers given water-cooled perches compared with those exposed to ambient perches. Total amount of feed consumed and total feed efficiency were only moderately affected (P less than or equal to .10) by perch treatments. These results indicated that water-cooled perches were beneficial in improving broiler performance during periods of high environmental temperatures.

Animals↗

PubMed1992

Effects of protein and amino acid level fed zero to forty-two days on response of broilers to protein and amino acid levels fed forty-two to forty-nine days of age.

Three studies were conducted to evaluate the effect of protein and amino acid (AA) levels fed 0 to 42 days posthatch on response of broiler chickens to levels of protein and AA fed during the finisher period. Birds were grown to 42 days on diets formulated to contain a minimum of 105 or 120% of recommended AA levels, and then randomly assigned to diets formulated to contain a minimum of 70, 80, 90, 100, 110, or 120% of recommended AA levels for 42 to 49 days. No minimum protein level was imposed on the diets. Body weight, feed intake, feed utilization, dressing percentage, abdominal fat content, and parts yield were determined. The protein and AA level fed 0 to 42 days had significant effects on 42- and 49-day body weight and 42- to 49-day feed intake of male but not female broilers. However, there was no interaction of protein and AA levels fed 0 to 42 days on response to protein and AA levels fed 42 to 49 days for these criteria. Weight gain, feed intake, and feed utilization of both males and females 42 to 49 days were significantly influenced by protein and AA levels fed during this time; protein and AA requirements of females during this time appeared to be equal to, or greater than, those of the male broiler. Although there were significant interactions of protein and AA levels 0 to 42 and 42 to 49 days on various carcass parameters for both male and female broilers, these followed no consistent pattern. Protein and amino acid levels fed 0 to 42 days had a significant influence on both quantity and percentage of abdominal fat in both male and female broilers processed at 49 days but not on any other carcass parameters. The protein and AA levels fed 42 to 49 days had a significant effect on both quantity and percentage of abdominal fat in male but not female broilers. The protein and AA levels fed 42 to 49 days significantly influenced quantity but not percentage of breast meat in male but not female broilers. The results of the current study indicate that there was minimal carryover effect of protein and AA levels fed 0 to 42 days on response to AA levels fed 42 to 49 days.

Adipose Tissue↗

PubMed1993

Early feed restriction of broilers: effects on abdominal fat pad, liver, and gizzard weights, fat deposition, and carcass composition.

A total of five experiments were conducted to investigate the effects of early feed restriction on organ weights, fat deposition, and carcass composition in broilers. In Experiments 1 and 2, broiler chicks were reared in litter pens for 49 days, whereas Experiments 3, 4, and 5 were conducted in battery cages for 28 days. Feed restriction in all experiments was accomplished by providing male broiler chicks 40 kcal per bird per day for 7 (Experiments 1 and 2) or 6 (Experiments 3, 4, and 5) days, starting at 4 days of age. Feed restriction of broiler pullets in Experiment 1 was imposed from 4 to 9 days of age. Furthermore, the starter and grower diets in Experiment 2 contained either 1 or 4% added fat and the starter diets in Experiments 3, 4, and 5 contained either 21 or 26% protein. No significant differences were observed for abdominal fat pad and gizzard weights, liver and carcass fat, or carcass protein between early restricted birds and ad libitum controls at 49 days of age in Experiments 1 and 2. Furthermore, with the exception of Experiment 4, no differences in abdominal fat pad weights were observed among the different feeding regimens. Significant increases in abdominal fat pad weights, percentage liver, and percentage carcass fat were noted for females at 49 days of age when compared with male broilers. Carcass fat was significantly higher and carcass protein was significantly lower in broilers fed commercial versus reduced fat diets. Broilers fed a 21% protein diet had significantly heavier abdominal fat pad weights at 28 days of age than birds fed a 26% protein diet.

Adipose Tissue↗

PubMed1996

Hemorrhages in muscles of broiler chickens: the relationships among blood variables at various rearing temperature regimens.

Hemorrhages in muscle tissue can be considered as major quality defects of broiler carcasses. They can be induced by stunning, especially electrical stunning. The underlying mechanism, however, is considered to be multifactorial. In this study, the effect of blood circulation disturbances on the severity of hemorrhages induced by electrical stunning was investigated. The disturbances were evoked in two genetically different, fast-growing broiler strains, Ross and Hybro, by rearing the broilers at low ambient temperatures. The broilers were slaughtered by two different electrical stunning methods. Broilers reared at low temperatures showed changes in blood variables and heart weight known to be associated with blood circulation disturbances. There was no effect of rearing temperature on hemorrhage severity. Ross broilers, being the most susceptible to low temperatures, had less severe hemorrhages than Hybro broilers. There was, however, a clear effect of the stunning method on hemorrhage severity. Whole body stunning caused more severe hemorrhages than head stunning in thigh and breast muscles. These results suggest that factors interfering with blood circulation have little or no effect on the occurrence of hemorrhages induced by electrical stunning.

Abattoirs↗

PubMed2003

Jerusalem artichokes stimulate growth of broiler chickens and protect them against endotoxins and potential cecal pathogens.

Control of intestinal pathogens during the earliest phases of broiler production may be the best strategy for the reduction of human pathogens on processed broiler carcasses. The recent ban on antibiotics in poultry feed has served to focus much attention on alternative methods of controlling the gastrointestinal microflora. A field trial was conducted to evaluate the effect of the fructan-rich Jerusalem artichoke, or topinambur (administered as 0.5% topinambur syrup in drinking water), on cultural numbers of selected cecal bacteria (total aerobes, Enterobacteriaceae, Bdellovibrio spp., and Clostridium perfringens) and levels of bacterial endotoxins as well as on body weights and relative weights of organs (the pancreas and the bursa of Fabricius) of chickens in the first 35 days of life (with weekly investigations being conducted). One-day-old broiler chickens (Ross 308) were randomly assigned to experimental (with topinambur) and control (without topinambur) groups. They were allowed free access to a standard broiler diet without growth-promoting antibiotics. Topinambur treatment resulted in a significant increase (P < 0.01) in cecal counts of B. bacteriovorus, which parasitizes susceptible gram-negative pathogens. Topinambur led to significantly smaller numbers of total aerobes, Enterobacteriaceae, and C. perfringens as well as to reduced levels of endotoxins in the blood compared with those for control birds. Increased body weights resulting from topinambur consumption were observed on day 35 of the trial period (P < 0.05). The relative weights of the pancreas and the bursa of Fabricius, however, were higher (P < 0.05) for topinambur-treated broilers than for control birds at the ages of 14, 21, 28, and 35 days. These results indicate that a small amount of topinambur in broilers' drinking water has a beneficial effect on growth performance, reduces bacterial endotoxin levels, and suppresses potential pathogens in broilers' ceca.

Animal Feed↗

PubMed2006

Prevalence and genetic characterization of Listeria monocytogenes in retail broiler meat in Estonia.

The prevalence and genetic diversity of Listeria monocytogenes in raw broiler legs at the retail level in Estonia were studied. A total of 240 raw broiler legs (120 from Estonia and 120 of foreign origin, which had been imported to Estonia from Denmark, Finland, Hungary, Sweden, and the United States) from 12 retail stores in the two largest cities in Estonia (Tallinn and Tartu) were investigated from January to December 2002. Of these, 70% were positive for L. monocytogenes. The prevalence of L. monocytogenes in broiler legs of Estonian origin (88%) was significantly higher than in broiler legs of foreign origin (53%) (P < 0.001). Altogether, 169 (106 Estonian and 63 imported) L. monocytogenes isolates were characterized by pulsed-field gel electrophoresis (PFGE) typing after treatment with the restriction enzyme AscI. The isolates showed a wide genetic diversity, with 35 different PFGE types obtained. Of these, 11 PFGE types came only from isolates of broiler legs of Estonian origin, 4 of Danish origin, 2 of Finnish origin, and 4 of Hungarian origin. Fourteen PFGE types came from isolates of broiler legs that originated from various countries. The strains that shared the same PFGE types from isolates of Estonian origin were recovered from broiler legs that came from different stores over the course of several months. Seventy-one L. monocytogenes isolates, including all PFGE types, were serotyped, and three serotypes (1/2a, 1/2b, and 4b) were obtained. Serotype 1/2a accounted for 96% of the isolates.

Animals↗

PubMed1999

The MHC of a broiler chicken line: serology, B-G genotypes, and B-F/B-LB sequences.

Although the major histocompatibility complex of chickens (encoded in the B complex) has been studied for a number of years, almost all work has focused on the White Leghorn breed. Broiler (meat-type) chickens were derived from other breeds, including Cornish and Plymouth Rock. It was our hypothesis that new B haplotypes, not previously identified in White Leghorns, might be present in lines of broiler chickens. Furthermore, alloantisera used to identify B serotypes in Leghorn lines reportedly do not work well outside the line in which they were raised, with the result that broiler B haplotypes have not been incorporated into the universal nomenclature system. Our approach was to use a panel of B alloantisera produced to identify B serotypes within a commercial broiler breeder line (designated line A). B homozygotes identified serologically were compared by B-G genotyping using restriction fragment length polymorphism analysis. Furthermore, reverse transcription-polymerase chain reaction was used to amplify variable domains of expressed B-LB and B-F genes of homozygotes of most of the B serotypes in Line A, followed by cloning and nucleotide sequence determination. Comparison of B-LB and B-F sequences with standard Leghorn haplotypes demonstrated the existence of new alleles of B-L and B-F in a broiler breeder line, as well as the presence of alleles previously identified in Leghorns. In some cases, Leghorn-type alleles were in linkage with different B-G alleles in the broiler line than the common haplotypic associations found in Leghorn lines.

Alleles↗

PubMed2006

Heat production and lipid metabolism in broiler and layer chickens during embryonic development.

We compared heat production (HP) and lipid metabolism in broiler and layer chickens (Gallus gallus) during embryonic development. To investigate HP and respiratory quotient (RQ), oxygen (O2) consumption and carbon dioxide (CO2) production were measured using an open-circuit calorimeter system. HP consistently had a tendency (P = 0.06) to be lower in broilers than in layers during embryonic development, and HP gradually decreased with developmental stage in both strains. RQ values of both strains were approximately 0.7 at every embryonic stage investigated. These results suggest that chicken embryos mainly use lipid for energy, and the RQ was significantly lower in broilers than in layers during embryonic development. Consumption of the yolk sac as a lipid source was faster in broilers than in layers. Plasma D-3-hydroxybutyrate (D3HB) and glycerol concentrations, associated with fatty acid oxidation, were lower in broiler than layer embryos. These results demonstrate that HP and lipid metabolism are different between the strains during embryonic development, and may be one factor for the growth difference between broiler and layer embryos.

3-Hydroxybutyric Acid↗

PubMed2006

Immune responses in Eimeria acervulina infected one-day-old broilers compared to amount of Eimeria in the duodenum, measured by real-time PCR.

T-cell responses are supposed to be the major immune reactions in broilers infected with Eimeria. The nature of such T-cell responses is influenced by the species of Eimeria involved, age of the host, amount of parasites and the preceding infection history. In young chicks the intestine is still developing in length while the lymphocyte populations in the gut develop and differentiate. In chicks infected at young age the immune response may be different in quality as compared to responses in adults. We investigated the (T-cell) immune responses of young broilers to a primary Eimeria acervulina infection in relation to the number of parasites used for infection. In our experiment we infected one-day-old broilers with a low (5 x 10(2) oocysts) and a high (5 x 10(4) oocysts) dose of E. acervulina. We used a newly developed species specific real-time PCR to quantify total amount of parasites in the duodenum as the number of oocysts in faeces may not be representative for the exposure of the gut immune system. We characterized T-cell subsets in the duodenum by means of FACS-analyses, lymphocyte proliferation assays with spleen lymphocytes and the mRNA profiles of different cytokines (TGF-beta2, -4, IFN-gamma, IL-2, -6, -8 and -18) in the duodenum by means of real-time PCR. From day 5 p.i. broilers with a high dose of E. acervulina had a significantly lower body weight than the control group. No increase in CD4(+) cells, but a strong increase in CD8(+) cells was observed at days 7 and 9 p.i. in the duodenum of broilers infected with a high dose E. acervulina. IL-8 mRNA responses were observed after infection with low and with high infection doses, but no IFN-gamma and TGF-beta mRNA responses were found in the duodenum. The specific proliferative T-cell responses to a low infectious dose were not significantly different as compared to the control group. In conclusion, based on the kinetics of observed responses a primary infection with a high dose of E. acervulina in one-day-old broilers seems to generate an immune response that shows a peak at the time of oocyst excretion, whereas the immune response to a low dose is less explicit.

Animals↗

PubMed2004

Presence of Enterococcus faecalis in broiler litter and wild bird feces for bacterial source tracking.

When Enterococcus faecalis is isolated from fresh feces, its host range appears to be limited to humans and birds. Although E. faecalis is found in human sewage, the extent to which the bacterium is found in broiler litter and in the feces of wild birds is unclear. These results have implications for bacterial source tracking. We determined if media designed for the isolation of fecal enterococci affected this host range, and if E. faecalis was routinely found in broiler litter and in the feces of wild birds. Of five different isolation media, none affected the isolation of E. faecalis. Enterococcus faecalis was routinely found in fresh broiler feces (522 of 1092 isolates; 48%), but rarely in broiler litter (12 of 1452 isolates; <2%). Therefore, broiler litter selects against this bacterium, and broiler litter is an unlikely environmental source of this bacterium. The presence of E. faecalis in eight wild bird species was highly variable. Unless the fecal loading rate from migratory or resident wild birds is high, water samples collected during baseflow conditions with high numbers of E. faecalis may indicate human fecal contamination.

Animal Husbandry↗

PubMed2000

Persistence of cellulitis-associated Escherichia coli DNA fingerprints in successive broiler chicken flocks.

Avian cellulitis in broiler chickens is primarily caused by Escherichia coli. Previous research found that the E. coli isolates of cellulitis origin were unique to each ranch, suggesting that these E. coli were endemic within the ranch environment. To test the hypothesis that the E. coli associated with cellulitis are endemic in the litter of the broiler house, we designed a study to determine whether E. coli DNA fingerprints associated with cellulitis persist over successive flocks that are grown in the same house. In addition, we assessed the impact of different cleaning and disinfection strategies on this persistence. Two broiler houses were followed on each of five farms over 3-4 flocks. A total of 353 E. coli isolates from cellulitis lesions were analyzed in this study, and 314 of these isolates (89%) were DNA fingerprinted by PFGE. In each ranch, there were several DNA fingerprint patterns that were present over successive flocks, regardless of the cleaning and disinfection strategy utilized. Isolates persisted as long as 191 days, implying that these E. coli are capable of persisting in the broiler house environment for long periods of time. In addition, these E. coli isolates were associated with cellulitis lesions in successive flocks. Thus, the isolates of E. coli that are associated with cellulitis in broiler chickens appear to be endemic in the litter environment of the broiler house.

Animal Husbandry↗