PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Desensitization, Immunologic”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 271 records · Page 15Linked to original sources
PubMedDate not supplied

The influence of CpG motifs on a protein or DNA-based Th2-type immune response against major pollen allergens Bet v 1a, Phl p 2 and Escherichia coli-derived beta-galactosidase.

BACKGROUND: DNA immunization and protein immunization with CpG motifs as adjuvants represent promising approaches in allergen-specific immunotherapy. OBJECTIVE: We investigated the effect of coinjection or prepriming with CpG-ODN on Th2-type responses induced by gene gun and protein immunization. METHODS: BALB/c mice were immunized with the gene gun using plasmid DNA containing the cDNAs coding for the genes of Bet v 1a, Phl p 2 and beta-galactosidase or with the purified Al(OH)(3)-adsorbed proteins. In addition, CpG-ODN were applied by coinjection or by prepriming treatment. Antibody and cytokine responses were measured by ELISA, proliferative and cytotoxic responses were determined by standard labeling procedures. Furthermore, the allergenic activity of sera was measured by passive cutaneous anaphylaxis. RESULTS: Gene gun immunization and protein immunization induced a clear Th2-type response for all antigens. The Th1-promoting effect of CpG-ODN coinjection together with gene gun immunization was restricted to beta-galactosidase as indicated by the increase of IgG2a and a marked expression of IFN-gamma. CpG motifs also increased the specific cytotoxic response against beta-galactosidase. Prepriming with CpG-ODN and gene gun or protein immunization with Bet v 1a exhibited no significant difference to the non-CpG control group. However, sera from mice preprimed with CpG-ODN induced no anaphylaxis with gene gun immunization, but with protein immunization. CONCLUSIONS: The effect of CpG motifs in vivo depends on a variety of parameters like the nature of the antigen and the immunization modality. Furthermore, our studies indicate that a combination of CpG + DNA immunization may be more effective in antagonizing Th2 responses than the combination of CpG + protein immunization.

Adjuvants, Immunologic↗
PubMed1963

[PEDIATRICS].

Explore the source record for details and available documents.

Child↗
PubMed1965

EMULSION THERAPY FOR ALLERGIC DISEASE. CLINICAL RESULTS WITH USE OF SMALL VOLUMES.

In a series of cases in which emulsion repository desensitization therapy for allergic disease was carried out, the amount of emulsion injected was, for a time, one milliliter. Then, for a second period, the volume was reduced to 0.5 milliliter, and minor refinements of technique were introduced. Many of the patients treated in the first period were also treated in the second. The incidence of untoward reaction, ranging from mild soreness to the development of draining cysts at the site of injection into the arm, was greatly reduced between the first and second periods.

Biomedical Research↗