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Effects of brown midrib 3 mutation in corn silage on productivity of dairy cows fed two concentrations of dietary neutral detergent fiber: 1. Feeding behavior and nutrient utilization.

The effects of digestibility of corn silage neutral detergent fiber (NDF) and dietary NDF content on feeding behavior, dry matter intake (DMI), and energy utilization were evaluated with eight multiparous high producing dairy cows in a duplicated 4 x 4 Latin square design with 21-d periods. Experimental diets contained corn silage from a brown midrib (bm3) hybrid or its isogenic normal control at two concentrations of dietary NDF (29 and 38%). Both low NDF diets and bm3 corn silage treatments increased DMI and milk yield. However, an interaction between corn silage treatment and dietary NDF content was observed for meal size and for interval between meals, implying that different mechanisms regulating DMI dominate depending on the fermentation characteristics of diets. The bm3 treatment depressed milk fat concentration when fed in a low NDF diet. The bm3 corn silage increased solids-corrected milk yield, but did not affect daily body weight gain compared with control, whereas low NDF diets increased daily body weight gain, but did not affect solids-corrected milk yield compared to high NDF diets. Both bm3 treatments and low NDF diets reduced ruminal pH, but low NDF treatments increased fluctuation of ruminal pH and bm3 treatments did not. Feeding bm3 corn silage increased the energy utilized for milk production, possibly because of a consistent supply of metabolic fuels from the rumen. The beneficial effects of bm3 corn silage on productivity of lactating cows were greater for the cows fed a high NDF diet.

Animals↗

Impact of dengue virus infection on feeding behavior of Aedes aegypti.

In addition to heavily infecting the salivary glands of Aedes aegypti (L.) mosquitoes, dengue viruses produce a significant infection of the nervous system, involving the brain, Johnston's organ, compound eye, and thoracic and abdominal ganglion. To determine if dengue infection affects feeding behavior of Ae. aegypti we measured feeding times, counted the number of feeding delays or interruptions, and by in situ immunocytochemistry techniques determined the spatial and temporal distribution of dengue infections in females parenterally infected with dengue 3 virus. The mean of the total time required for feeding by infected mosquitoes was significantly longer than the time required by uninfected mosquitoes. Similarly, the mean of the time spent probing was significantly longer in infected mosquitoes than in uninfected mosquitoes when day after inoculation was considered. Significant increases in the length of feeding activity in infected mosquitoes corresponded to virus infection in organs that are known to control or influence activities associated with blood feeding. Sequential infections of the salivary glands (five days postinoculation [PI]), brain and compound eye (eight days PI), and Johnston's organ and midgut and abdominal ganglion (11 days PI) of most mosquitoes were observed. The increased time required by infected Ae. aegypti mosquitoes to acquire a blood meal may contribute to the efficiency of Ae. aegypti as a vector of dengue virus. Longer feeding periods are more likely to be interrupted by the host, which increases the chance that an infected mosquito will probe or feed on additional hosts.

Aedes↗

A contact eatometer for automated continuous recording of feeding behavior in rats.

A new contact eatometer designed and built in our laboratory is described. The system makes possible the reliable continuous recording of feeding behavior in rats from the time of weaning, without significantly affecting food intake- and growth-related variables. Improved time discrimination and reliability of records--along with the small financial outlay necessary to manufacture the device--make the system appropriate for chronobiological studies which involve the simultaneous use of a large number of animals.

Animal Feed↗

[The integrative activity of cortical neurons in the systemic organization of feeding behavior].

Unit activity of neurons in the visual and parietal brain cortical areas was recorded in cats in the course of feeding behavior. The cells had individual discharge patterns at successive stages of behavior. Rearrangements of patterns in the cortical areas under study depended on modality of a conditioned stimulus, feeding motivation level, and efficiency of the obtaining of intermediate results of behavioral acts. The distribution of impulse flow at the nerve cell axon reflects the dynamics of its integrative activity determined by preceding state of the neuron and current synaptic activation against the background of development of key mechanisms of the functional system of the behavioral act.

Acoustic Stimulation↗

GABA in the nucleus accumbens shell participates in the central regulation of feeding behavior.

We have demonstrated previously that injections of 6, 7-dinitroquinoxaline-2,3-dione into the nucleus accumbens shell (AcbSh) elicits pronounced feeding in satiated rats. This glutamate antagonist blocks AMPA and kainate receptors and most likely increases food intake by disrupting a tonic excitatory input to the AcbSh, thus decreasing the firing rate of a population of local neurons. Because the application of GABA agonists also decreases neuronal activity, we hypothesized that administration of GABA agonists into the AcbSh would stimulate feeding in satiated rats. We found that acute inhibition of cells in the AcbSh via administration of the GABAA receptor agonist muscimol or the GABAB receptor agonist baclofen elicited intense, dose-related feeding without altering water intake. Muscimol-induced feeding was blocked by coadministration of the selective GABAA receptor blocker bicuculline, but not by the GABAB receptor blocker saclofen. Conversely, baclofen-induced feeding was blocked by coadministration of saclofen, but was not affected by bicuculline. Furthermore, we found that increasing local levels of GABA by administration of a selective GABA-transaminase inhibitor, gamma-vinyl-GABA, elicited robust feeding in satiated rats, suggesting a physiological role for endogenous AcbSh GABA in the control of feeding. A mapping study showed that although some feeding can be elicited by muscimol injections near the lateral ventricles, the ventromedial AcbSh is the most sensitive site for eliciting feeding. These findings demonstrate that manipulation of GABA-sensitive cells in the AcbSh can have a pronounced, but specific, effect on feeding behavior in rats. They also constitute the initial description of a novel and potentially important component of the central mechanisms controlling food intake.

Animals↗

Reduced competition and altered feeding behavior among marine snails after a mass extinction.

Extinction may alter competitive interactions among surviving species, affecting their subsequent recovery and evolution, but these processes remain poorly understood. Analysis of predation traces produced by shell-drilling muricid snails on bivalve prey reveals that species interactions were substantially different before and after a Plio-Pleistocene mass extinction in the western Atlantic. Muricids edge- and wall-drilled their prey in the Pliocene, but Pleistocene and Recent snails attacked prey only through the shell wall. Experiments with living animals suggest that intense competition induces muricid snails to attack shell edges. Pliocene predators, therefore, probably competed for resources more intensely than their post-extinction counterparts.

Animals↗

[Angiotensin II in the organization of rat feeding behavior].

Intraventricular injection of angiotensin-II (100 ng) increased the latency period and decreased the amount of food intake in the hungry rats. Preliminary administration of saralasin (100 ng) blocked the inhibitory effect of angiotensin-II on food consumption. In contrast to the above mentioned intraperitoneal injection of angiotensin-II (10 ng/kg) 10 min before food and water admission results in the tendency to increase the food intake. And the same application of angiotensin-II 60 min before food and water admission increased by 49% of food intake in hungry rats. Saralasin (50 ng/kg) given prior to the angiotensin-II administration blocked the angiotensin-II effects. Moreover a significant decrease in the food intake was found after intraperitoneal injection of saralasin only. No significant changes in the drinking behavior in response to the intraventricular and intraperitoneal drugs administration were registered.

Angiotensin II↗

Feeding behavior of bollworm and tobacco budworm (Lepidoptera: Noctuidae) larvae in mixed stands of nontransgenic and transgenic cotton expressing an insecticidal protein.

Feeding behavior of third-instar bollworm, Helicoverpa zea (Boddie), and tobacco budworm, Heliothis virescens (F.), was observed in pure and mixed stands of nontransgenic and transgenic cotton (BTK), Gossypium hirsutum L., expressing an insecticidal protein CryIA(c) from a bacterium, Bacillus thuringiensis Berliner subsp. kurstaki. Five plant stands composed of BTK and non-BTK plants were evaluated; two pure stands and three mixed stands. Percentage ratios of BTK to non-BTK plants in the stands were 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. In all stands with BTK plants, fewer bollworm and tobacco budworm larvae were found on BTK plants than non-BTK plants 24 h after infestation with third instars. At 48 h, significantly fewer tobacco budworm larvae, but not fewer bollworm larvae, were found on BTK plants. However, the number of larvae of either insect did not increase on non-BTK plants compared with the initial infestation density of three larvae per plant. The number of obacco budworm injured flower buds, and capsules was lower in all plant stands containing BTK plants compared with the pure stand of non-BTK at 48 h after infestation. Higher numbers of larvae on non-BTK plants were possibly the result of larval intoxication, reduced feeding, and increased plant abandonment and death on BTK plants rather than a classical feeding preference. Unexpectedly, the number of flower buds and capsules injured by bollworm and tobacco budworm when averaged per plant for all plants in a stand, differed little among the 75:25, 50:50 and 25:75 plant mixtures. These data suggest that larvae of both species frequently moved among plants, feeding indiscriminately on BTK and non-BTK plants.

Animals↗

Acute effects of knowledge-based work on feeding behavior and energy intake.

The aim of this study was to evaluate the impact of knowledge-based work (KBW) on feeding behavior and spontaneous energy intake with the use of a repeated measures/within-subjects design. We used a two-session protocol including an ad libitum buffet preceded by either rest in a sitting position for 45 min or a cognitive task (reading a document and writing a summary of 350 words using a computer) over the same time period. In this regard, 15 healthy Laval University female students (mean age = 24.1+/-2.2 years, mean BMI = 24.0+/-4.3 kg/m2) were recruited to participate in this study. Anthropometric variables, energy expenditure, heart rate, blood pressure, food intake (dietary record), and appetite sensation markers were measured at each testing session, and two questionnaires [Three-Factor Eating Questionnaire (TFEQ) and State-Trait Anxiety Inventory (STAI)] were administered. In addition, a buffet-type meal was used to measure spontaneous energy intake and macronutrient preferences. We found that the mean energy expenditure of the two conditions was about comparable (difference of 13 kJ between the two tasks) whereas the mean ad libitum energy intake after the KBW task exceeded that measured after rest by 959 kJ (p < 0.01). Although a higher absolute energy intake was observed for the three macronutrients after the KBW task (p < 0.05), no specific preference was detected, as reflected by the comparable percent of energy from each macronutrient in the two conditions. No significant difference in appetite sensation markers was observed between the two conditions, although the subjects ate more in the buffet-type meal after the KBW task. Furthermore, the subjects did not compensate by decreasing food intake for the rest of the day, suggesting a net caloric surplus. We also observed negative correlations between cognitive dietary restraint (TFEQ) and spontaneous energy intake in both conditions. In conclusion, our results demonstrate that mental work solicited by KBW has the potential to induce a higher spontaneous energy intake. This also raises the possibility that KBW adds a new component to sedentariness that might further accentuate the positive energy balance that is more likely to occur when one is inactive.

Adult↗

Breast-feeding in preterm twins: Development of feeding behavior and milk intake during hospital stay and related caregiving practices.

In a prospective study of 13 preterm twins still in the hospital, 85% were breast-fed, of which 46% were breast-fed exclusively. Most mothers preferred simultaneous breast-feeding, using the football hold. Observations and maternal descriptions showed differences between the twins in their development of breast-feeding behavior, especially in sucking. The mothers' suggestions regarding special support for the breast-feeding mothers of preterm twins involved synchronizing feeding with the twins' behavioral states; twin cobedding; appropriate armchairs and breast-feeding pillows; experimenting with breast-feeding positions; information about breast milk production; nurses' spontaneous practical assistance, encouragement, and emotional support; the provision of privacy; the availability of parent rooms; and opportunities for fathers' presence in the hospital.

Adult↗

Frequent blood-feeding and restrictive sugar-feeding behavior enhance the malaria vector potential of Anopheles gambiae s.l. and An. funestus (Diptera:Culicidae) in western Kenya.

Natural blood-feeding and sugar-feeding behaviors were investigated for populations of Anopheles gambiae s.l. and An. funestus Giles at 2 sites in western Kenya. During peak levels of malaria parasite transmission, > 85% of 1,569 indoor-resting females contained fresh blood meals. Findings that up to 55.4% of blood-fed resting females and 72.0% of host-seeking females had either stage IV or V oocytes provided strong evidence that females were refeeding before oviposition. Such gonotrophic discordance was common throughout the year for both An. gambiae s.l. and An. funestus. Determinations of gonotrophic cycles for freshly blood-fed mosquitoes collected inside houses indicated that only 60.0% of 1,287 An. gambiae s.l. and 60.0% of 974 An. funestus oviposited eggs after a single blood meal. The timing of oviposition was irregular as indicated by relatively high coefficients of variation for An. gambiae s.l. (44.0%) and An. funestus (35.9%). Associated with frequent blood feeding was a surprisingly low rate of sugar feeding; only 6.3% of 1,183 indoor-resting and only 14.4% of 236 host-seeking anophelines were positive for fructose. Natural patterns of frequent blood feeding, year-round gonotrophic discordance, irregular oviposition cycles, and limited sugar feeding illustrate that anopheline mosquitoes have complex behavioral and physiologic means for adapting to their environment. In western Kenya, for example, adaptations for frequent blood feeding by An. gambiae s.l. and An. funestus potentiates their ability to transmit malaria parasites, well beyond that predicted by standard measures of vectorial capacity.

Animals↗

Effects of chronic restraint stress on feeding behavior and on monoamine levels in different brain structures in rats.

Monoaminergic systems are important modulators of the responses to stress. Stress may influence feeding behavior, and the involvement of monoamines in the control of food intake is well recognized. We investigated the effects induced by chronic-restraint stress, 1 h a day, for 40 days, on eating behavior and on monoamines in distinct brain structures. Increased consumption of sweet pellets, and not of peanuts, was observed. Dopamine (DA), serotonin (5-HT), and their metabolites were measured by HPLC-EC. After chronic restraint, the results observed were decreased 5-HT in hippocampus, with increased 5-HIAA/5-HT; decreased 5-HIAA levels in cortex; reduction in DA in hippocampus, and increased levels in amygdala and hypothalamus; HVA increased in cortex, as well as HVA/DA ratio, while DOPAC/DA decreased. HVA decreased in hypothalamus, as well as HVA/DA, and DOPAC/DA and HVA/DA decreased in the amygdala. These results suggest that restraint stress differentially affects the activity of central dopaminergic and serotonergic neurons, and this may be related to the effects observed in eating behavior.

Animals↗