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PubMed2006

Amycolatopsis australiensis sp. nov., an actinomycete isolated from arid soils.

The taxonomic position of a group of mesophilic actinomycetes isolated from arid Australian soils was determined using a polyphasic approach. The organisms shared chemical and morphological markers typical of members of the genus Amycolatopsis. They had identical 16S rRNA gene sequences and formed a distinct phyletic line in the Amycolatopsis mediterranei clade, being most closely related to A. mediterranei. In addition, they shared a range of phenotypic properties that distinguished them from representatives of all of the species classified in this clade. The combined genotypic and phenotypic data indicate that the strains merit species status within the genus Amycolatopsis. The name proposed for the novel species is Amycolatopsis australiensis sp. nov.; the type strain is GY048(T) (=DSM 44671(T)=NCIMB 14142(T)).

Actinomycetales↗
PubMed1996

Identity-by-descent mapping of recessive traits in livestock: application to map the bovine syndactyly locus to chromosome 15.

Twelve animals affected with syndactyly or mulefoot were sampled in the Dutch black-and-white cattle population. Analysis of the pedigree data reveal that all of these individuals traced back to a single acknowledged carrier founder individual. Between seven and nine generations separated the founder from its 12 affected descendents. The 12 affected offspring were genotyped for a battery of 213 microsatellites spanning the 29 bovine autosomes. The resulting genotypes were analyzed using a maximum likelihood approach searching for shared homozygous haplotypes among affected individuals. Three candidate regions for the syndactyly locus emerged from this initial screening. syndactyly was shown to map to one of these candidate regions on chromosome 15 by genotyping 29 additional individuals linking founder and affected offspring and performing a conventional linkage analysis with the LINKAGE programs. This study illustrates the potential of identity-by-descent mapping in livestock populations.

Animals↗
PubMed1994

Issues in phenomenology for researchers of nursing.

Nursing today, with its individualistic approach to care, shares many of its underlying beliefs and values with the school of philosophical thought known as phenomenology. The research method derived from phenomenology considers that the true meaning of phenomena can only be explored through the experience of them as described by the individual. This paper explores the features of phenomenology as a research method in relation to its relevance for nursing by first discussing the philosophical underpinnings of the method, and then proceeding to an examination of the features of the method with relation to research design, and data analysis. The specific issues of validity and generalizability are developed further, with reference to the criticisms levelled at the phenomenological method from the alternative quantitative paradigm. Finally, the author discusses the relevance of phenomenology for nursing, and concludes that, as a research method within the qualitative paradigm, it has a great deal to offer nursing as a humanistic discipline.

Data Interpretation, Statistical↗
PubMed1998

In vitro and in vivo properties of various types of platelets.

The most popular procedures used to prepare platelet concentrates (PC) can be categorized into methods using platelet rich plasma (PRP) obtained by soft centrifugation of whole blood units, methods using buffy coats (BC) obtained by hard centrifugation of whole blood units, and apheresis procedures. The main feature that differentiates apheresis PC from whole blood unit derived PC is that apheresis reduces the number of donors necessary to support a recipient, although the advantages related to this feature have not been conclusively documented. From the biochemical point of view, a limited number of comparative studies and a large series of non-comparative studies indicate that differences in PC obtained with different protocols depend more on the performances of the production laboratory rather than the preparative approach--PRP, BC or apheresis--itself. Similarly, the clinical effectiveness of PC seems to depend more on PC age, storage modalities and recipient's conditions rather than primarily on the method of PC preparation. Despite the basic differences in the three preparative approaches, all methods share a number of key elements that are necessary pre-requisites for a successful platelet transfusion therapy: minimization of the risk of bacterial contamination by careful donor skin disinfection and temperature control during PC production and storage; protection of aerobic metabolism during PC storage; laboratory quality control including at least pH and volume determinations, platelet and white cell counts and visual inspection of the swirling phenomenon at time of release. Future directions in the field of PC production include the development of new products such as infusible platelet membranes, thrombospheres, thromboerythrocytes and reconstituted freeze-dried platelets.

Adult↗
PubMed2005

Managing attention deficit/hyperactivity disorder: unmet needs and future directions.

The management of attention deficit/hyperactivity disorder (ADHD) requires a multimodal approach. Although shared-care protocols are suggested, uptake by general practice has been tentative. Behavioural management, both on its own and in combination with pharmacotherapy, reduces core symptoms and associated behaviours, such as oppositional defiant disorder (ODD). Links with education and social support are also important for helping children and their families. Psychostimulants have shown to be effective in the treatment of ADHD, however, their short duration of action highlights potential limitations. Other drugs used in the management of ADHD and comorbid disorders include antidepressants, clonidine and its analogues, newer atypical neuroleptics, and atomoxetine, a novel, non-stimulant therapy that has potential to fulfil some unmet treatment needs. Further research is needed in preschool children, girls, those with epilepsy, young adults, and in those with associated sleep disturbance. Selected rating scales and careful history taking, allied to close links with schools, are vital for the initial and ongoing assessment of ADHD and its comorbidities.

Adolescent↗
PubMed2004

Cost-effective violence prevention through targeted family interventions.

Efforts in violence prevention can focus on individual youth, their families, their schools, or the communities in which they live. Among the small number of program models that have been proven effective in repeated replications, those that focus on improving family management and child care have been found to be the most cost-effective. The four model programs include Nurse Home Visitation, Functional Family Therapy, Multi-systemic Therapy, and Multidimensional Foster Care. All of these programs involve detailed protocols, extensive staff training and supervision, and quality-assurance procedures. The factors limiting their wider adoption include staff resistance to their structured approach, cost-sharing issues between local and state levels of government, and the political power of existing programs.

Adolescent↗
PubMed1998

The 1st annual meeting of the American Society of Gene Therapy.

The inaugural meeting of the American Society of Gene Therapy (ASGT) attracted over 1700 participants to the Pacific gateway city of Seattle, Washington for a multifaceted 4 day meeting organised into a series of symposia, workshops, poster sessions and educational opportunities representative of gene therapy's immense diversity. Presentations from the international assemblage of industrial and academic scientists covered a blend of data from cutting edge research to current clinical investigations across a spectrum of therapeutic targets. Unique educational sessions allowed participants to gain basic information regarding areas of gene therapy research in which they lacked familiarity, whereas several 'Meet the Investigator' sessions allowed participants to interact directly with experts in small group settings in order to obtain a more sophisticated perspective through informal dialogue. A large majority of the symposia and poster presentations showcased the development and current successes of viral-mediated gene therapy although non-viral approaches received their share of attention. Despite the fact that promising results were presented from ongoing clinical trials using viral-mediated gene therapy, much of the symposia addressed the accompanying problems of immunogenicity, low levels of gene expression and lack of control of gene expression that are presently limiting the clinical success of virally mediated gene transfer.

Journal Article↗
PubMed2006

Financial preservation and protection for the elderly.

This article's objective is to share various financial approaches, facts, and opinions that may be helpful to those assisting the elderly. This document has four main sections: a tool kit of ideas for dealing with financial affairs; investment facts, financial planning, and recommendations--a road map for planning and investing; long-term care insurance--who might benefit, and; toward the end, steps to take to preserve wealth when life expectancy is short.

Aged↗
PubMed1975

Challenge to international agencies.

International health agencies face major changes requiring basic adjustments in approaches and values. The ethical issues include moral criteria for allocating scarce resources, relation of health to population growth and development, iatrogenic social consequences of health measures, and inappropriate transfer of technology. A proposed new style of international health work is summarized in five principles and ten guidelines. The principles are: development from below; a role shift from adviser collaborator; sequential research, demonstration, and implementation; concentration on problems of motivation; and parternship in approaches to mutually shared complex problems.

Developing Countries↗
PubMedDate not supplied

An analysis of the epistemic orientation of first- and third-year students in a physiotherapy program.

Previous research has demonstrated that practitioners within the same profession share a common approach to knowledge acquisition and transmission. A search for the identification of this common approach to knowledge in physiotherapy was undertaken using the Epistemic Orientation Model. The research reported was premised on the assumption that students admitted to physiotherapy programs in Canada possess an epistemic orientation that is similar to the epistemological structure of the knowledge base considered important to the program. Using a standardized instrument known as the Knowledge Accessing Modes Inventory (KAMI) to measure epistemic orientation, data were collected from a sample (N = 59) of first- and third-year students registered in a physiotherapy program in a central Canadian university. The data indicated that a high degree of association exists between the epistemological structure subsumed in the knowledge base deemed essential to gain admission to the program and the dominant rational epistemic mode of the practitioners-to-be. Based on the results, the article examines the usefulness of the epistemic orientation concept on activities within the profession. Implications for professional development are noted and avenues for further research are suggested.

Canada↗
PubMed1984

A DRG-based management information system.

In the June 1984 issue of Pathologist, Larry Shaw, MD, encouraged pathologists to turn threat into opportunity by coming partners with hospital management in addressing prospective payment challenges. He cited his own experience at Desert Samaritan Hospital and Health Center of Mesa, Ariz., as an example of how pathologists could participate in setting policy. The author of the following article shares yet another approach.

Costs and Cost Analysis↗
PubMedDate not supplied

Long-term care: workable options for employers now.

Lost in the labyrinth of long-term care benefits possibilities (or impossibilities)? There are ways that employers can begin today to provide at least a modicum of long-term care coverage for retired (and active) employees--and at very little cost. A long-term care consultant shares some practical approaches, along with insights into how federal government activities may affect employers' efforts.

Aged↗
PubMed2004

[Shared decision-making in primary breast cancer].

Following an overall view of the emotional situation of primary breast cancer patients and of studies exploring shared decision making in this setting we present the results of qualitative analyses about breaking bad news situations and interviews with this group of patients and describe both methods for implementing shared decision making in the treatment of primary breast cancer and an evaluation study. The main result was that most patients showed a lack of information about breast cancer and its therapy, which led to uncertainty. Another source of uncertainty was the lack of preoperative punch biopsy. Due to the so-called "diagnostic shock" patients felt under pressure to make a quick decision over their treatment. This perceived pressure can be effectively countered by a shared decision making approach. To investigate the options in breast cancer therapy, decision aids for diagnostic and therapeutic options have been developed focusing on the following treatment modalities: 1. breast conserving therapy and radiation versus radical mastectomy 2. chemotherapy plus endocrine therapy versus endocrine therapy alone 3. preoperative versus postoperative chemotherapy. The decision instruments in question were tested in N = 35 breast cancer patients and N = 36 nurses as a control group. The results of a questionnaire survey among both these groups revealed a very positive appraisal of the decision aids. Implementation in daily routine was favoured.

Breast Neoplasms↗
PubMedDate not supplied

Parent and nurse partnership model for teaching therapeutic relationships.

The development of trusting, caring, boundaried, mutual relationships between staff, patients, and families in health care settings demands a set of staff skills that reflect an understanding of the perspective of patient/family as well as one's colleagues. When nurse leaders collaborate with families in teaching nurses, they offer a powerful means of gaining an understanding of the complexities inherent in the health care partnership. This shared nurse leader/family approach to education provides an opportunity to apply relationship principles to real life care situations and creates a forum for discussion of the various perspectives.

Attitude of Health Personnel↗
PubMed2005

Integrating the Modelling of EN 1828 and Galen CCAM Ontologies with Protégé: towards a Knowledge Acquisition Tool for Surgical Procedures.

The presentation assess the usability of the ontology platform protégé integrated with the terminology reasoning tool RACER to represent different terminology systems as the CEN European standard EN 1828 which is a categorical structure and the extensive French coding system CCAM supported by a GALEN representation. We present the 2 systems and some results showing the easiness to test the consistence of the ontology or of instances of terminology systems.This type of software tool which is accessible as open source could support a convergent "reference terminology representation" approach. Based on a formal representation development and allowing diversity in linguistic expressiveness of end users this approach can associate shared knowledge acquisition in the public domain and competing systems, software developers and researchers.

Humans↗
PubMedDate not supplied

Staff development: an alternative to inservice education.

Continuing education may be viewed from two perspectives. Inservice programs are often perceived as externally mandated activities oriented toward the correction of deficiencies. In contrast, the staff development approach involves the shared decision making of the participant and supervisor in the planning, implementation, and evaluation stages of the learning process. Research indicates that the philosophical and managerial perspectives associated with staff development enhance efforts at implementing change.

Education, Medical, Continuing↗
PubMedDate not supplied

DOC as an integral part of the community medicine curriculum.

Involving family practice residents in community medicine experiences can be a challenging task, and various programs have approached this curricular problem in different ways. The Cedar Rapids program has integrated the activities of the local Doctors Ought to Care (DOC) chapter into the residency with the goal of exposing residents to the concepts of community health education. DOC, a national organization interested in community health promotion and education, takes a lively approach to information sharing using image-based techniques.

Community Medicine↗