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Results for “CELLOPHANE”

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At least 289 records · Page 16Linked to original sources

PubMed2001

[Formation of ethyl carbamate in umeshu (plum liqueur)].

Samples of umeshu, a Japanese plum liqueur made from unripe plums, shochu and crystal sugar, were stored under fluorescent light, in the dark and in the refrigerator. The amount of ethyl carbamate formed in umeshu exposed to light or room temperature was larger than that in the dark or at low temperature. The amount of ethyl carbamate formed in umeshu to which cyanide had been added was larger than that in the absence of added cyanide. Thus, the amount of ethyl carbamate formed in the umeshu was increased by not only light and higher temperature, but also cyanide. Samples of model alcoholic beverages were stored under various conditions using red, yellow and blue cellophanes. The amount of ethyl carbamate formed in the model alcoholic beverage with blue cellophane was larger than in the cases of red and yellow cellophanes. It was found that the amount of ethyl carbamate formed in the model alcoholic beverage was increased by light in the wavelength range of 375-475 nm.

Alcoholic Beverages↗

PubMed2005

[The distribution of intestinal parasites in students of the Mustafa Kemal University School of Health.].

Intestinal parasites are an important national health problem in our country as in the rest of the world. In our study, the prevalence of intestinal parasites in female students (aged from 16-18 years) in the Mustafa Kemal University School of Health was investigated. Fecal samples and cellophane tape preparations were used for diagnosis. For this reason 142 fecal samples and 136 cellophane tape preparations were examined. One or more parasites were found in 65 (45.77%). fecal samples. The prevalence of parasites that found in fecal samples is as follows: Blastocystis hominis in 63 samples (96.92%) and Giardia intestinalis in 2 samples (3.08 %). Enterobius vermicularis was found in 9 (6.61%) out of 136 cellophane tape preparations.

English Abstract↗

PubMed2005

[The prevalence of intestinal parasites in four different special daytime nursing homes and day-centers in Antakya.].

In this study, the prevalence of intestinal parasites in 109 students (68 boys, 41 girls) in the 1-6 age group in four different special daytime nursing home and day-centers (Special Anakucaği Day-time Nursing Home, Special Evim Day-centre and Day time Nursing home, Special Doğa Daytime Nursing Home and Special Yaren Daytime Nursing Home) in the Antakya district of Hatay was investigated. For this reason 86 feces and 109 cellophane tape preparations were investigated. Concentration of feces and cellophane tape methods were used for diagnosis. One or more parasites were detected in 18 (20.93%) out of 86 concentrated fecal specimens. Eight (7.40%) Enterobius vermicularis were detected in 109 cellophane tape specimens . The prevalence of parasites in concentrated specimens included 4 (19.04%) Giardia intestinalis, 12 (57.14%) Blastocystis hominis, 4 (19.04%) Entamoeba coli and 1 (4.76 %) Hymenolepis nana.

English Abstract↗

PubMed1978

Migration of leukocytes to human nasal mucosa.

In order to perform their antimicrobial function PMN must be able to migrate from capillaries to tissues and mucusal surfaces. We found that cellophane squares placed against the nasal septum can stimulate a local cellular response resulting in the migration of granulocytes to the surface of the squares. Upon removal of the squares, adherent cells can be stained, identified, and quantitated. Studies in which the cellophane squares are left against the septum for 5 min reveal a dense monolayer of granulocytes (greater than 6 per 70 micrometer2) on only 7% +/- 2 of the surface of the square. After 2 hr against the nasal septum, a dense monolayer of granulocytes was found on 87% +/- 10 of the surface of the cellophane square. This technique provides a simple and noninvasive method for in vivo measurement of leukocyte migration.

Adult↗

PubMed1980

[Immobilized luciferase from the fireflies Luciola mingrelica. Changes in the pH-dependence of the enzyme catalytic activity and stability during its immobilization on various polysaccharide carriers].

The luciferase from the fireflies luciola mingrelica was immobilized on different CNBr-activated polysaccharide carriers, e. g. Sepharose, Ultrodex, cellophane, Ultrogel. The catalytic activity and the inactivation rate constants of the soluble and immobilized enzyme at 25 degrees (pH 6.0--9.0) were determined. During immobilization on Sepharose the pH profile of activity shifts towards lower pH values, while upon immobilization on Ultrogel and Ultrodex it is considerably broadened. Immobilization on Ultrogel and Ultrodex results in a 3--100 -fold stabilization of the enzyme (pH less than 7.5). whereas the stability of luciferase immobilized on Sepharose and cellophane within the same pH range is 10--1000 times higher than that of the soluble enzyme. It was shown that inactivation of luciferase immobilized on Sepharose and cellophane is limited by oxidation of the SH-groups of the enzyme and is inhibited by dithiothreitol. The inactivation of luciferase immobilized on Ultrogel and Ultrodex and that of soluble enzyme is not limited by oxidation of the SH-groups.

Animals↗

PubMed1997

[Prevention abdominal contamination by napkin in performance of colonic anastomosis].

A special napkin for performance of colonic anastomosis is proposed. The napkin represents a piece of cellophane with the side cuttings, ending with 2 orifices of 3 cm in diameter. 2 tubes are installed into the piece of cellophane on the upper and lower sides. The mobilized part of the colon and the tumor are put into the napkin. The napkins edges are being fixed to the surgical sheets. The parts of the colon, that are being planned to resect, are isolated from the abdominal cavity and are located in the cellophane bag. After accomplishing the first row of sutures a flowing irrigation of the anastomosed parts of colon is being done. The use of the napkin prevents polluting the abdominal cavity and abdominal wall wound.

Anastomosis, Surgical↗

PubMed1978

Microbial biodegradation of cellophase.

The microbial biodegradation of cellophane (U.C.B.--Division Sidac) was studied. Preliminary experiments with pure cultures of seven cellulolytic microorganisms (Aspergillus sp., Penicillium sp., Chaetomium crispatum, Ch. globosum, Sclerotium rolfsii and two actinomycetes) revealed that the substrate as such was very recalcitrant, probably due to the occurrence of insoluble coating agents. Therefore, mixed cultures of the above mentioned cellulolytic microorganisms were used as inoculum. The cellophane showed a slow microbial degradation which starts only after 37 days of incubation. This long lag-phase is due to the unaltered presence of the coating agents. However, when the coating agents are extracted with tetrahydrofuran, the biodegradation starts after 10 days, resulting in a biodegradation rate of 85% after 52 days of incubation and a protein content of 30%. The endproduct (30% protein, 60% soluble sugars, 10% residual substrate) will probably be useful as compost.

Actinomycetales↗

PubMed1992

Expression of growth factors in a pancreatic islet regeneration model.

In this model of pancreatic regeneration, the initial trophic effect of cellophane wrapping is upon the ductular-epithelium, as shown by autoradiographic analysis following a single pulse of 3H-TdR. The uptake of 3H-TdR by ductular cells is maximal approximately 4 weeks before the peak uptake of the label by islet cells. Six weeks after the pulse, most of the label is contained in differentiating islet cells, not ductular cells. This phenomenon suggests that wrapping of the pancreas induces "progenitor" cells to differentiate along the line of endocrine cells. The essentially negative observations on REG gene expression in the regenerating hamster pancreas indicate that the message for this gene is either not involved in the regeneration process or that it is sufficiently different from the rat gene (a rat control probe was used) that it precludes detection by our methodology. It might be necessary therefore, to generate a hamster REG specific probe in order to further study this issue. In conclusion, the established model of cellophane wrapping of the pancreas provides a useful tool whereby the induced growth of beta-cells can be observed. The sequence appears to be initial stimulation of ductal cells followed by islet differentiation. Of the candidate genes, it appears that reg does not participate in the process. We will however, examine other candidate genes to elucidate the mechanism whereby celophane wrapping induces growth of beta-cells.

Animals↗

PubMed2000

The effect of doramectin, moxidectin and netobimin against natural infections of Syphacia muris in rats.

In this study, the effect of doramectin, moxidectin and netobimin was investigated in naturally infected Syphacia muris in rats. The natural infection was determined by the use of cellophane tape method on the perianal region and by the technique of centrifugal flotation of feces. The infected rats were divided into three treated and one control group (N = 10). Doramectin and moxidectin at the dose of 0.2 mg/kg per day and netobimin at the dose of 7.5 mg/kg per day were given in the diet for 4 days. Cellophane tape preparations were performed in all groups on 4th and 7th day after the last treatment. The rats of treated groups were necropsied on 7th day after the last treatment together with that of control group. While doramectin and netobimin were highly effective against S. muris, moxidectin was not found to be effective for eradication of S. muris.

Animals↗

PubMedDate not supplied

Membrane-based cell affinity chromatography to retrieve viable cells.

A novel scheme for the separation and live recovery of one cell type from a mixture of cells using a cell affinity chromatography (CAC) system is demonstrated. An anti-murine IgG was chemically immobilized to a cellophane support via a carbonyldiimidazole (CDI) link. Murine splenocytes flowed over the support, and B-cells were allowed to attach at a shear rate of 15 s-1. Once loading was terminated, the support was washed at a shear rate of 315 s-1 to remove nonspecifically bound cells. Elution of the B-cells was initiated by the transmembrane diffusion of hydrochloric acid (pH 1), supplied to the side of the membrane opposite the cells. At the same time, a shear flow of normal saline was established on the cell side of the membrane, and cells, freed by acid, were retrieved. Results showed that, on average, 250 cells/mm2 attached to antibody immobilized on cellophane surfaces, at a shear rate of 15 s-1, and that attached cells were successfully displaced by acid supplied to the side of the membrane opposite that holding the cells. On average, at least 60% of the B-cells removed by this elution appeared viable, based on a Trypan Blue dye exclusion assay.

Animals↗

PubMed2000

Influence of the physical state of water on the barrier properties of hydrophilic and hydrophobic films.

Water transfer through different films, as a function of the physical state of water in contact with the film, the relative humidity difference, and the water vapor pressure difference, was investigated. The films were two synthetic packagings (hydrophobic polyethylene and hydrophilic cellophane) and an edible film. The physical state of water affects water sensitive films, such as cellophane, inducing a higher liquid water transfer due to interactions with the polymer. For hydrophobic polymers, such as polyethylene, neither the physical state of water nor the relative humidity has an influence on the water permeability. In complex system, such as an edible film composed of hydrophilic particles dispersed in a lipid phase, barrier efficiency is influenced by the continuous hydrophobic phase but could also be affected by the physical state of water due to the presence of hydrophilic compounds.

Cellophane↗

PubMed1977

Complement and leukocyte-mediated pulmonary dysfunction in hemodialysis.

During hemodialysis, cardiopulmonary decompensation may appear in uremic patients, possibly caused by plugging of pulmonary vessels by leukocytes. In 34 patients we noted leukopenia (20% of initial levels) during hemodialysis that in 15 was associated with impaired pulmonary function. When we infused autologous plasma, incubated with dialyzer cellophane, into rabbits and sheep, sudden leukopenia and hypoxia occurred, with doubling of pulmonary-artery pressures and quintupling of pulmonary-lymph effluent. Histologic examination showed severe pulmonary-vessel-leukostasis and interstitial edema. The syndrome was prevented by preinactivation of complement but was reproduced by infusions of plasma in which complement was activated by zymosan. Thus, acute pulmonary dysfunction from complement-mediated leukostasis may play a major part in the acute cardiopulmonary complications of cellophane-membrane hemodialysis.

Adolescent↗

PubMed1983

Renal blood flow and glomerular filtration rate in renal wrap hypertension in rabbits.

Serial measurements of mean arterial pressure and glomerular filtration rate (GFR) were made in rabbits before and 10, 28 and 56 days after bilateral renal cellophane wrap (n = 8) or sham operation (n = 6). No significant changes were seen 10 days after renal wrap, but by 28 days GFR was reduced by 50 +/- 3% and mean arterial pressure had risen by 42 +/- 5 mmHg. No significant further changes occurred over the subsequent month. Changes in plasma renin activity after renal wrapping were not significantly different to those seen after sham operation. In a separate series of experiments, renal extraction ratio of para-aminohippurate (PAH), 28 days after renal wrap, averaged 63 +/- 4% (n = 6) compared to 83 +/- 2% (n = 5) after sham operation. Calculated renal blood flow of the hypertensive rabbits was only about 40% of that in the sham operated rabbits. Thus large reductions in GFR, renal blood flow and PAH extraction ratio occurred following bilateral renal wrapping and these changes may reflect compression of the kidney by the cellophane induced fibrous capsule. These reductions occurred concomitantly with the rise in arterial blood pressure.

Animals↗

PubMed1993

In vitro stimulation of hamster pancreatic duct growth by an extract derived from the "wrapped" pancreas.

Partial obstruction of the hamster pancreas in the cellophane wrap model leads to the induction of duct and ductular proliferation followed by endocrine cell differentiation. This effect appears to be mediated by the local action of a growth factor. The purpose of the present study was to determine if cytosolic extract prepared from the wrapped pancreas had trophic activity on purified hamster pancreatic ductal epithelium in tissue culture. Cultures of purified pancreatic ducts were prepared by digestion of the hamster pancreas using a solution of collagenase type XI and chymotrypsin infused directly into the pancreatic duct. The ducts were separated and purified by a series of steel mesh filtrations. Ducts were embedded in 1.5% Seaplaque agarose and fed a liquid medium containing serum-free Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham (DME/F-12), 12.5% cytosol extract+DME/F-12, or 25% cytosol extract+DME/F-12. The trophic effect of the extract on the tissue in culture was evaluated by the incorporation of tritiated thymidine ([3H]TdR) into DNA. Duct fragments cultured in medium supplemented with 12.5% cytosol showed no difference in their [3H]TdR uptake compared with control ducts (908 +/- 147 vs. 913 +/- 151 dpm/micrograms DNA). The incorporation of [3H]TdR by ducts maintained in medium supplemented with 25% cytosol extract was increased 78% over serum-free controls (1,632 +/- 386 vs. 913 +/- 147 dpm/micrograms DNA; p < 0.025). We conclude that a cytosol extract prepared from the partially obstructed cellophane-wrapped pancreas contains a factor(s) trophic for pancreatic ductal cells.

Animals↗

PubMed2002

An observational study of the management of traumatic tympanic membrane perforations.

Controversies of how best to treat fresh tympanic membrane perforations have always existed. While some otolaryngologists prefer the paper patch method, others prefer modified myringoplasty. A prospective study is needed to investigate the most effective and least expensive management of this common ear trauma. This study examined prospectively, in three sections, a group of patients with a cellophane patch (n = 6), another group with a gentamicin ointment seal (n = 15) and a control group (n = 9) with a gentamicin plug placed at the distal end of the external auditory cavity. Successful healing of the traumatic tympanic membrane perforations was achieved in 50 per cent of the cellophane seal group, 86.7 per cent of the gentamicin ointment seal group and 77.8 per cent of the control group. This study shows that the management of a fresh tympanic membrane perforation should be limited to cleaning the traumatized ear and preventing infection.

Administration, Topical↗

PubMed1975

[Healing of the donor area after transplantation of splitted grafts].

The healing of wounds in the donor area of splitted grafts was observed. The transplants served to graft an already existing wound which had not healed spontaneously. 120 wounds sustained by 95 patients were examined either histologically or by Rebuck's method of skin fenestration. 30 wounds were dressed with ointment and tulle, in 55 cases the wound was covered with perforated cellophane and an Allen-Koch compression bandage. These patients also received 1500 mg Complain in 500 ml physiological saline solution daily, by continuous drip infusion, for three days postoperatively. In 35 patients the wounds were only covered with perforated cellophane, but without additional medication. The application of Complamin shortened the healing process to seven to ten days in contrast to the control group where the wounds took 15 to 17 days to heal.

Aged↗

PubMed1991

The mechanical test method of cardiovascular and related biomaterials.

Mechanical testing methods were developed by us using the pulsatile internal pressure through-flow system. The methods were applied to estimate the permeability and fatigue strength of a vascular substitute, the mechanical behaviour of anastomosed site between vascular substitute and blood vessel, and the mechanical behaviour of the blood vessel itself. A testing method is also proposed for puncturing a vascular substitute which is used as blood access for hemodialysis. The mechanical behaviour of punctured vascular substitute and the puncturing technique itself are investigated. The fatigue-creep testing method of cellophane membrane is also reported to estimate the durability of cellophane membrane by using the circulating pulsatile internal pressure through-flow system. The mechanical testing method for visco-elastic characteristic of biomaterials is discussed.

Anastomosis, Surgical↗

PubMed1975

Role of the "thrombocytoids" in capsule formation in the dipteran Calliphora erythrocephala.

Of the three hemocyte types present in the blood of Calliphora, only one participates in capsule formation around implanted cellophane. This hemocyte, the thrombocytoid, shows in the blood a tendency to dissociate into numerous small cytoplasmic fragments, comparable to the mammalian megakaryocyte. This tendency is dramatically increased during the process of encapsulation. Most of the intact thrombocytoids and the numerous fragments participating in capsule formation do not show any particular modifications in their cytoplasm during this process, which corresponds to a mere sequestration of the implant. Dense material, resulting from necrotic cell debris and hemolymph lipoproteins, is often observed between the cellophane and encapsulating thrombocytoids, which apparently participate in the resorption of this material.

Agglutination↗