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At least 289 records · Page 16Linked to original sources
PubMed1981

[Is aplastic anaemia a pre-leukaemic state? (author's transl)].

Out of 412 adult patients classified as aplastic anaemia in a co-operative study group, 22 developed acute leukaemia from 3 months to 5 years after the initial diagnosis. In 21 of these, retrospective analysis elicited one or several clinical and laboratory data which are uncommon in aplastic anaemia but common in pre-leukaemic refractory anaemia. Indeed, one half of these patients should have been classified as pre-leukaemic, and in the remaining half cell kinetics investigations (analysis of radioactive iron renewal, stem cell cultures and 3H-TdR autoradiography of bone marrow), as well as cytogenetic studies, might have led to a more correct classification. Only one patient, who initially had none of the criteria of pre-leukaemic state, responded to androgen treatment with a 4-year complete remission but died on the 5th year of acute myeloblastic leukaemia. It appears from this retrospective analysis that when all signs of pre-leukaemia have been excluded by thorough investigation, aplastic anaemia is never, or only exceptionally (1 out of 412 cases), a pre-leukaemic condition.

Adolescent↗
PubMed1976

Effects of prostaglandins A2, E2 and F2 alpha on erythropoietin production.

The erythropoietic effects of the renal prostaglandins were investigated in exhypoxic polycythemic mice and in isolated perfused dog kidneys. Prostaglandins A2 and E2 in a dose range of 100 to 800 mug/kg b.wt. were found to produce a dose-related increase in radioactive iron incorporation in red cells. Prostaglandin F2 alpha was devoid of an erythropoietic effect in polycythemic mice. In that PGE2 is the principal renal prostaglandin, PGE2 (0.5 mug/min) or saline was infused into isolated perfused dog kidneys that had been programmed by prior exposure to hypoxia (0.42 atm) for 4 hours. PGE2-infused kidneys during 5 hours of perfusion with blood from nonhypoxic donor dogs produced significant increases in perfusate titers of erythropoietin when compared with that of saline infusion controls at the same time intervals. These data suggest an involvement of the renal prostaglandin PGE2 in erythropoietin production by the kidney.

Animals↗
PubMed1981

The growth-promoting effect of bacterial iron for serum-exposed bacteria.

Bacterial ability to obtain iron in bovine serum or in media containing transferrin (Tr) or conalbumin (Ca) was investigated by using serum-resistant (virulent) and serum-sensitive (avirulent) strains of Escherichia coli and Salmonella typhimurium. Bacteria growing in bovine serum enriched with radioactive iron-saturated Tr or with radioactive iron-saturated enterobactin (E) did not acquire radioactive iron. It has been found that the passage of siderophore (Si)-iron complexes into bacteria is blocked in serum by Tr and in Ca-containing medium by Ca. The investigation of bacterial ability to take iron in synthetic media showed that bacteria take in Si-bound but no Tr-bound radioactive iron. In the absence of free iron, the growth of serum-exposed virulent bacteria was supported by their stored iron. Virulent bacteria passaged in medium void of usable iron became depleted in stored iron and did not grow in animal sera unless sera were enriched by the addition of exogenous iron. Experiments with serum-exposed avirulent bacteria showed that their growth in Si-enriched serum should not be attributed to the iron-providing activity of Si but to the stimulating effect of Si which facilitates the use of stored iron. As distinct from avirulent bacteria, virulent bacteria used stored iron without the stimulating activity of extracellular Si.

Blood↗
PubMed1994

Iron-58 and neutron activation analysis: a non-radioactive method for tracing hemoglobin iron.

To develop a non-radioisotopic alternative to radioactive Fe-59 as a hemoglobin iron tracer, we evaluated Fe-58 and neutron activation analysis (NAA). Upon irradiation with thermal neutrons, stable isotope Fe-58 is converted to radioactive Fe-59 through (n,gamma) neutron reaction; its radioactivity measured by a gamma counter is proportional to the original Fe-58 present. Organ samples from rats infused with Hb or saline were analyzed along with a 10 mg Hb as a standard (105 nanograms of Fe-58). The measured values of Fe-58 in tissues were compared with those derived from the literature tissue iron contents. The amount of Fe-58 in normal tissue is extremely low yet detectable by NAA demonstrating high sensitivity. Measured values of Fe-58 from tissues were generally correlated well with literature derived values (e.g., brain: 0.19 and 0.15 ng/mg tissue; kidney: 0.6 and 0.7 ng/mg tissue, respectively). The results indicate that Fe-58 can be used as a non-radioactive tracer for absorption, distribution, metabolism, and distribution studies of hemoglobin-based oxygen carriers.

Animals↗