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At least 289 records · Page 16Linked to original sources
PubMed2004

[Study on preparation of beta-cyclodextrin inclusion complex of volatile oil in xiongdan xiaoyan capsules].

OBJECTIVE: To study optimum inclusion conditions for volatile oil from three medicinal slices in Xiongdan Xiaoyan capsules. METHOD: Saturated water solution method (agitation method), ultrasonic method and rubbing method were studied and compared. The preparation conditions of ultrasonic method were investigated by the orthogonal test. RESULT: The optimum inclusion conditions of ultrasonic method were established. CONCLUSION: The ultrasonic method can be used for production of inclusion complex in the factory with high inclusion rate and was effectiveness.

Angelica↗
PubMed2003

[Studies on the chemical constituents of the volatile oil from Radix Cudramiae].

OBJECTIVE: To study the chemical constituents of the volatile oil from Radix Cudramiae. METHOD: The oil was obtained by steam distillation. The chemical compositions were separated and identified by GC-MS. The relative contents in the oil were determined by area nomalization. RESULT: Sixty-four compounds were obtained from Radix Cudramiae. The relative contents of twenty-three compounds were more than 1%. The highest relative content was L-linalool (9.852%). CONCLUSION: Sixty-four compounds characterized by GC-MS analysis were obtained from Radix Cudramiae for the first time.

1-Octanol↗
PubMed2003

Effects of elephant garlic volatile oil (Allium ampeloprasum) and T-2 toxin on murine skin.

Effects of elephant garlic (Allium ampeloprasum) volatile oil (GVO) and trichothecene (T-2) toxin were studied in Swiss albino mice. The animals were 1) topically applied GVO, 2) topically applied T-2 toxin, 3) topically applied GVO followed by T-2 toxin (GVO/T-2), and 4) T-2 toxin application followed by GVO (T-2/GVO) on the right footpad. All animals were observed by Langerhans cell enumeration and pathological changes of the footpad on days 1, 3, 5 and 7. The number of Langerhans cells in the GVO treated group (1,097 +/- 33/mm2 to 1,624 +/- 19/mm2) was not significantly different when compared with the corresponding control left footpad (1,143 +/- 33/mm2 to 1,674 +/- 21/mm2). Langerhans cells density in T-2 toxin treated group (629 +/- 29/mm2to 1,090 +/- 31/mm2) was reduced by 20-35% of the opposite control footpad (962 +/- 40/mm2 to 1,392 +/- 29/mm2). Furthermore, GVO/T-2 and T-2/GVO treated mice showed a decrease in Langerhans cell number than a single T-2 toxin treated group. While Langerhans cells in T-2 toxin, GVO/T-2 and T-2/GVO groups revealed a smaller cell size with shortening dendritic processes when compare to the normal control group. Histopathological findings of the footpad skin in T-2 toxin treated group revealed epidermal desquamation and necrosis with edema and inflammatory cells infiltration. While GVO/T-2 and T-2/GVO showed a similar sequence but a lesser severe degree. These findings suggested that GVO both in pre- and posttreatment could protect T-2 toxin induced epidermal damage in a mouse footpad.

Animals↗
PubMed2000

[Studies on technology of supercritical-CO2 fluid extraction for volatile oils and saikosaponins in Bupleurum chinense DC].

OBJECTIVE: To study the technology of supercritical-CO2 fluid extraction (SFE-CO2) for the volatile oils and saikosaponins in Bupleurum chinense. METHOD: Exploring the effects of pressure, temperature, extraction time, flow rate of CO2 and entrainers on the yield of the oils and saikosaponin-contained extracts; determining the optimum conditions for SFE-CO2; analyzing the oils by GC/MS and comparing the technology of SFE-CO2 with that of traditional steam distillation. RESULT: The optimum extraction conditions turned out to be--for volatile oils: pressure (EP) = 20 MPa, temperature (ET) = 30 degrees C, isolator I pressure (1P-I) = 12 MPa, temperature(1T-I) = 65 degrees C, isolator II pressure (1P-II) = 6 MPa, temperature (1T-II) = 40 degrees C, extraction time = 4 hours, and CO2 flow rate = 10-20 kg.(h.kg)-1 crude drug; for saikosaponins: EP = 30 MPa, ET = 65 degrees C, 1P I = 12 MPa, 1T I = 55 degrees C, 1P II = 6 MPa, 1T II = 43 degrees C, extraction time = 3 hours, entrainer = 60% ethanol, and CO2 flow rate = 20-25 kg.(h.kg)-1 crude drug. CONCLUSION: SFE-CO2 excels the traditional steam distillation in raising yield and reducing extraction time. The oils are composed of 22 constituents including caproaldehyde, and the saikosaponins can only be extracted with the help of entrainers under higher pressure and temperature.

Bupleurum↗
PubMedDate not supplied

Volatile oil composition of the aerial parts of Ajuga orientalis L. from Iran.

The volatile oil content and composition of the aerial parts of Ajuga orientalis L. (Lamiaceae) grown in northern parts of Iran have been analyzed by GC/MS. Thirty compounds were identified, representing 97.9% of the total oil. The main compounds were germacrene-D (24.2%), beta-cubebene (18.3%), beta-caryophyllene (16.9%) and alpha-cubebene (5.3%).

Ajuga↗
PubMed1999

[GC-MS analysis of volatile oil of herba Pogostemonis collected from Gaoyao county].

The chemical constituents of the volatile oil of the stems and leaves of Pogostemon cablin collected from Gaoyao county, Guangdong province, have been studied by means of GC-MS. Sixty four compounds have been identified. The main constituents are pogostone (30.99% in stems, 21.31% in leaves, the same below), patchouli alcohol (10.26%, 37.53%), transcaryophyllene (4.92%, 6.75%), alpha-guaiene (2.27%, 6.18%) and seychellene (1.56%, 1.99%), etc.

China↗
PubMed1989

[Studies on the chemical constituents of Aquilaria sinensis (Lour) Gilg. III. Elucidation of the structure of isobaimuxinol and isolation and identification of the constituents of lower boiling fraction of the volatile oil].

A new sesquiterpenoid, named isobaimuxinol, C15H26O2, mp 73-75 degrees C, [a]D12-68(0) (c 0.10, CHCl3), was isolated from the volatile oil of Aquilaria sinensis (Lour), Gilg. (Thymeleaceae). Based on spectral (IR, 1H-NMR, 13C-NMR and 2D-NMR as well as MS) analysis its structure was identified as isobaimuxinol. The relative stereochemistry of isobaimuxinol was determined by X-ray crystallograhy. In addition, four known compounds, benzylacetone, p-methoxybenzylacetone, anisic acid and beta-agarofuran were isolated and identified from the lower boiling fraction of the volatile oil of this plant. These compounds were obtained for the first time from this plant.

Chemical Phenomena↗
PubMed2005

Chemical composition of the volatile oil from Cynanchum stauntonii and its activities of anti-influenza virus.

The volatile oil of the roots of Cynanchum stauntonii was examined by gas chromatography-mass spectrometry (GC-MS). Thirty-eight constituents were identified. (E,E)-2,4-Decadienal, 3-efhyl-4-methypentanol, 5-pentyl-3H-furan-2-one, (E,Z)-2,4-decadienal and 2(3H)-furanone,dihydro-5-pentyl were found to be the major components. The volatile oil exhibited the activities against influenza virus in vitro (IC50s=64 microg/ml). In in vivo experiment, it prevented influenza virus-induced deaths in a dose-dependent manner.

Animals↗
PubMed2005

[Classification of terpenes and terpene oxides in volatile oil of Fruit of Acanthopanax senticosus (Rupr. Et Maxim) Harms with gas chromatographic retention parameters].

Gas chromatographic (GC) retention parameters (A and B values) for 24 volatile compounds in volatile oil of Acanthopanax senticosus (Rupr. Et Maxim) Harms were obtained from the retention times under five temperature-programming conditions with self-developed GC_AB software based on Levenberg-Marquardt method. The correlation analysis between A and B parameters of terpene and its oxides was carried out. Good linear relationships between A and B parameters were built for seven monoterpenes (C10H16) , nine sesquiterpenes (C15H24), three monoterpene oxides (C10HnO) and three sesquiterpene oxides (C15HnOx) at the same carbon number, respectively. But poor A-B linearity was built for the group formed from monoterpenes and monoterpene oxides or that formed from sesquiterpenes and sesquiterpene oxides at the same carbon number. At the same time the A-B relationships for monoterpenes, sesquiterpenes and their oxides are not collinear. Therefore, this discipline is applied in the assistant identification for terpenes with different carbon numbers and in the classification between terpenes and their oxides. It shows that GC retention parameters calculated from retention times under several temperature-programming conditions are useful for the classification of monoterpenes, sesquiterpenes and their oxides in the analysis of volatile oils from traditional Chinese herbs.

Chromatography, Gas↗
PubMed2004

Application of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry in the analysis of volatile oil of traditional Chinese medicines.

This paper reports comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC x GC-TOF MS) analysis of Pogostemon cablin Benth (Cablin Parchouli) volatile oil. The suitable column system and operation conditions were chosen on the basis of the properties of composition of the volatile oil. One-dimensional gas chromatography (ID-GC) and GC x GC, GC-MS and GC x GC-TOF MS were compared under appropriate conditions, and the enhanced sensitivity and superior resolution of GC x GC were demonstrated. 394 components were tentatively identified by GC x GC-TOF MS.

Gas Chromatography-Mass Spectrometry↗
PubMed1992

[Effects of ginseng volatile oil on cytochemical components of SGC-823 gastric carcinoma in cell culture].

After 24, 48 and 72 hours' treatment of cells of SGC-823 gastric carcinoma in cell cultures with ginseng volatile oil, glycogen, succinate dehydrogenase and DNA in single cells were measured quantitatively with MPV2 microscope photometer. The results show that the inhibition of cancer cell growth by ginseng volatile oil may be due to the action of metabolism of DNA, carbohydrates and energy.

DNA, Neoplasm↗
PubMed2001

Chemical characterisation of the different types of volatile oil constituents by various solute retention ratios with the use of conventional and novel commercial gas chromatographic stationary phases.

Retention ratios of an unknown solute peak from a volatile oil can help to identify its chemical type. Relative retentions on conventional stationary phases can be used to calculate a polar/non-polar ratio which may suggest it is an aromatic solute or a sesquiterpene hydrocarbon. With dipentyl (DA) cyclodextrin phases, gamma-DA/alpha-DA ratios can indicate a bicyclic or monocyclic monoterpenoid. Temperature change on alpha-DA can pick out a non-alcoholic saturated cyclic monoterpenoid, using 150/125 degrees C. Twenty retention ratios are reviewed involving various phases, including other modified cyclodextrins, liquid crystals, "Chirasil-Val" and crown ethers. Applications to six volatile oils are made using a variety of phases.

Chromatography, Gas↗
PubMed2006

Analysis of Artemisia annua L. volatile oil by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry.

Artemisia annua L. is an annual herb native of Asia, it has been used for many centuries for the treatment of fever and malaria. In this paper, analysis of the volatile oil of Artemisia annua L. was performed by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC x GC-TOF MS). Three hundred and three components were tentatively identified and terpene compounds are the main components of Artemisia annua L. volatile oil. Artemisinic acid is tentatively qualified.

Artemisia annua↗
PubMed2001

[Chemical constituents in volatile oil from fruits of Alpinia oxyphylla Miq].

OBJECTIVE: To study the chemical constituents in the volatile oil from fruits of Alpinia oxyphylla. METHOD: Using GC-MS to identify the constituents. RESULT AND CONCLUSION: Sixty-four compounds were identified on the basis of GC-MS, the main ones being p-cymene, valence, linalool, myrtenal, alpha-pinene, beta-pinene, furopelargone A and terpinen-4-ol. Three sesquiterpenes valencene, nootkanone and nootkanol have been isolated from the CHCl3 extract as check, of these 64 identified compounds linalyl oxide, valencene, bakkenolide A, furopelargone A and 3-hydroxycalamenene are reported for the first time.

4-Butyrolactone↗
PubMed2004

[Determination of ligustilide in volatile oil from rhizome of ligusticum chuanxiong by RP-HPLC].

OBJECTIVE: To determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC. METHOD: ODS2 column (4.6 mm x 200 mm, 5 microm) was used and nitrendipine was used as internal standard. The mobil phase consisted methanol, acetontrile and water (33:21:46). The ligustilide was at 275 nm. RESULT: The linear range was 2.92-29.2 mg x L(-1) for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%. CONCLUSION: This method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility.

4-Butyrolactone↗
PubMed2006

[Effects of Rhizoma kaempferiae volatile oil on tumor growth and cell cycle of MKN-45 human gastric cancer cells orthotopically transplanted in nude mice].

OBJECTIVE: To evaluate the effects of Rhizoma kaempferiae volatile oil on tumor growth and cell cycle of MKN-45 human gastric cancer cells orthotopically transplanted in nude mice. METHODS: One hundred and five nude mice orthotopically transplanted with MKN-45 human gastric cancer cells were randomly divided into seven groups: untreated group, normal saline-treated group, dissolvant-treated group, cyclophosphamide (CTX)-treated group and high-, medium-, and low-dose Rhizoma kaempferiae volatile oil-treated groups. Corresponding interventions were implemented in each group except the untreated group. The antitumor effects in vivo were evaluated. Cell cycle distribution and apoptosis of MKN-45 human gastric cancer cells were determined by using flow cytometry (FCM). The ultrastructure of MKN-45 gastric cancer cells was observed by a transmission electron microscope. RESULTS: In the high-, medium-, and low-dose Rhizoma kaempferiae volatile oil-treated groups, the growth inhibition rates of gastric cancer were 57.2%, 28.0% and 5.0% respectively, and the gastric cancer cells were arrested at G(0)/G(1) phase. This antitumor effect was dose-dependent. The apoptotic cells occurred more frequently in the high-dose Rhizoma kaempferiae volatile oil-treated group and the CTX-treated group than those in the medium- and low-dose Rhizoma kaempferiae volatile oil-treated groups. CONCLUSION: The Rhizoma kaempferiae volatile oil is an effective composition for growth inhibition of gastric cancer, and its mechanism may be related to regulating the cell cycle and inducing apoptosis.

Animals↗