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At least 289 records · Page 16Linked to original sources
PubMed1999

Highly sensitive detection of swine vesicular disease virus based on a single tube RT-PCR system and DIG-ELISA detection.

A highly sensitive detection of swine vesicular disease virus (SVDV) based on a single tube RT-PCR system and digoxigenin (DIG)-PCR-ELISA detection was developed. Using a one tube RT-PCR system, optimisation of the PCR conditions and optimisation of the microwell hybridisation and colourimetric detection of the amplicons resulted in a method that could detect viral RNA in infected tissue culture fluid with a titre as low as 0.1 TCID50/100 microl. The same sensitivity was obtained with SVDV-spiked faeces, if the samples were pre-treated with 1,1,2-trichlorotrifluoroethane/chloroform and subsequently concentrated using an ultrafiltration system and RNA extracted with the Purescript kit. The specificity of the test was validated on 27 SVDV strains belonging to four different groups. No cross-reactivity with genetically and symptomatically related viruses was detected using RNA of foot-and-mouth disease virus (FMDV), porcine enterovirus (PEV), vesicular stomatitis virus (VSV), Coxsackie B5 virus (CV-B5) and encephalomyocarditis virus (EMCV). The test was validated successfully on clinical samples, being slightly more sensitive and much faster than virus isolation on cell cultures. Moreover the possibility of automating the procedure will allow the processing of large numbers of clinical samples.

Animals↗
PubMed2000

Chimeric swine vesicular disease viruses produced by fusion PCR: a new method for epitope mapping.

A new method of epitope mapping based on chimeric swine vesicular disease (SVD) viruses produced by fusion PCR (polymerase chain reaction). Seven out of 16 neutralising and non-neutralising newly produced monoclonal antibodies (MAbs) discriminated between SVD isolate ITL/1/66 and NET/1/92. Using fusion PCR eight chimeric viruses were produced containing different supplementary pieces of the P1 region of both parent strains. Using these chimeric viruses we were able to map the epitope regions recognised by these seven neutralising and non-neutralising Mabs. This new method, using chimeric viruses produced by fusion PCR, is particularly valuable for the epitope mapping of non-neutralising MAbs.

Animals↗
PubMed1974

Swine vesicular disease: comparative studies of viruses isolated from different countries.

Seven viruses isolated from outbreaks of swine vesicular disease in various countries between 1966 and 1973 were compared in pigs and infant mice. All produced a similar disease and virus excretion pattern in the pig, although the Italy/66 virus was considerably less virulent than the other viruses. The results of cross neutralization tests of convalescent pig sera and the response of 5-day-old mice to intraperitoneal inoculation indicated minor differences between some viruses. The Italy/66, Hong Kong/71 and France/73 viruses differed from each other and also from the Italy/72, England/72, Austria/73 and Poland/73 group of viruses.

Animals↗
PubMed1988

Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation for the diagnosis of foot-and-mouth and swine vesicular diseases.

An indirect, sandwich enzyme-linked immunosorbent assay (ELISA) has been compared in parallel with the standard complement-fixation typing test (CFT) for the diagnosis of foot-and-mouth and swine vesicular diseases. The superior sensitivity, reproducibility, economical use of reagents and ease of performance of the ELISA confirm the hypothesis that it should replace the CFT as the routine test of choice.

Animals↗
PubMed1985

[Respiratory diseases of swine: some epidemiologic aspects].

Epidemiological surveys enable to know better the aetiology of respiratory diseases. The surveys carried out in slaughter-houses in Brittany (France) in 1980 and 1981, show a worrying situation concerning porcine respiratory diseases. Microbiological studies brought out the preponderance of the isolation of Mycoplasma hyopneumoniae, Pasteurella multocida, Bordetella bronchiseptica, Streptococcus suis and Actinobacillus suis. Some patterns of experimental infection with Mycoplasma hyopneumoniae and Bordetella bronchiseptica are exposed.

Animals↗
PubMed1993

Complete nucleotide sequence of a coxsackie B5 virus and its relationship to swine vesicular disease virus.

We report the first complete nucleotide sequence of the picornavirus coxsackievirus B5 (CB5), strain 1954/UK/85, an isolate from a case of hand-foot-and-mouth disease. We have compared the sequence with those of other coxsackie B viruses, coxsackievirus A9, poliovirus and swine vesicular disease virus (SVDV). The genes encoding the three major capsid proteins are most closely related to those of SVDV but the 5' and 3' noncoding regions and the P3 gene are more similar to the corresponding regions in the other coxsackie B viruses than to those of SVDV. These observations are considered in the light of the antigenic and biochemical relationships between SVDV and CB5.

Amino Acid Sequence↗
PubMed1997

[Effect of immunostimulating agents on the occurrence of puerperal diseases in swine].

Investigations were done on effects of levamisol, chlormetine and biomast Cu. 22 upon the periparturient disease piglets. It was proved that levamisol and chlormetine applied prophylactically in immunostimulative doses at the last period of pregnancy exerted a favourable influence on the health state of sows after delivery. The piglets after sows previously subjected to immunostimulation with levamisol and chlormetine showed higher body mass gains per day, higher body mass on the 28th day after delivery, smaller number of diarrhoeas and death rate. A number of the reared piglets per one sow was also bigger. Preventive application of biomast administered at the end of pregnancy period turned out to be little effective in prophylaxis of periparturient diseases in sows. However, a positive influence of the above mentioned immunostimulation was stated in reference to some health and breeding indices.

Adjuvants, Immunologic↗
PubMed1989

An enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of antibodies against swine vesicular disease virus (SVDV).

A liquid phase blocking sandwich ELISA has been compared with virus neutralisation for testing pig sera for antibodies against swine vesicular disease (SVD) virus. Highest infectivity titre of SVD virus was obtained using a multiplicity of infection of 30 pfu/cell and harvesting after 21 h. Titres obtained for 300 clinically normal animals were assessed by ELISA and 89% were found to be 1/6 or less. Results were skewed and spread up to 1/45. Comparison of known positive sera resulted in a correlation between the two methods of 0.68 and showed that a virus neutralisation titre of 1/16 was equivalent to 1/40 (log10 1.61) by ELISA. Variation in results obtained by replicate testing using ELISA and virus neutralisation was almost identical. Overlap between positive and negative sera was shown to be reduced to 1-1/2 fold in ELISA. Therefore, the ELISA correlated well with virus neutralisation and has several advantages over the latter.

Animals↗
PubMed1997

Detection of swine vesicular disease virus RNA by reverse transcription-polymerase chain reaction.

Two polymerase chain reaction (PCR) assays are described for the detection of swine vesicular disease virus (SVDV) RNA, a reverse transcription PCR (RT-PCR) and a reverse transcription nested PCR (RT-nPCR). Both the RT-PCR and RT-nPCR were able to detect representative members of each of seven phylogenetically distinct groups of SVDV and gave negative results with a range of porcine enteroviruses and of viruses responsible for vesicular conditions in pigs. When combined with a commercial kit for rapid RNA extraction, the RT-PCR was useful for the detection of SVDV in samples of epithelium and faeces from animals with clinical SVD. The addition of a second amplification step to create a nested PCR (RT-nPCR) increased the sensitivity of the technique for the detection of viral RNA (vRNA) in SVDV infected tissue culture fluid by a factor of approximately 1,000, for 100 TCID50 for the RT-PCR to 0.1 TCID50 for RT-nPCR. When combined with a more elaborate extraction procedure for RNA, the RT-nPCR was considerably more sensitive than virus isolation in tissue culture for detecting SVDV in nasal swabs, tissues, and faeces collected from pigs between 7 days and 176 days after infection with a recent European isolate of SVDV. However, stringent conditions are necessary for carrying out the RT-nPCR to minimise the possibility of contamination.

Animals↗
PubMed1975

[Role of the virus of Aujeszky's disease in respiratory diseases of swine].

Studies carried out on pig-breeding farms have established the presence of Aujeszky's disease virus in cases of bronchopneumonia in pigs. On seven out of eight farms with bronchopneumonia the virus wad demonstrated both virologically and serologically, and nearly 50 per cent of the examined diseased pigs on these and other farms had antibodies. The individual serologic studies of pigs having antibodies revealed that the antibodies appeared in the second month of the animals' fattening. This corresponded to the manifestation of the initial symptoms of bronchopneumonia in the respective group.

Age Factors↗
PubMed1999

Identification of neutralizing epitopes on a European strain of swine vesicular disease virus.

Six neutralizing monoclonal antibodies (MAbs) were used to isolate MAb neutralization-resistant (MAR) mutants from a recent European strain of swine vesicular disease virus (SVDV), ITL/9/93. Sequencing of MAR mutants identified two epitopes located at positions analogous to sites 2A (VP2) and 3B (VP3) on poliovirus (PV) which have been previously identified on a Japanese strain of SVDV. A third epitope near to the C terminus of VP1, not previously recognized on SVDV, was tentatively identified in a region analogous to site 1 of PV. A fourth epitope, located in the C-terminal region of VP3, has never before been recognized as a site of neutralization on picornaviruses. All four epitopes were predicted to be surface-exposed.

Animals↗