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At least 307 records · Page 17Linked to original sources
PubMed1986

[Characteristics of human-mouse hybrid cell lines and the changes induced in them by the poliomyelitis virus].

Cells of the four hybrid lines between continuous mouse cells Rag and human diploid embryonal fibroblasts were polymorphic and had mitotic activity in fully formed monolayers. Most of the these mitoses were pathological. Hybrid cells examined 8 months after hybridization were susceptible to the poliomyelitis virus infection with partial cytopathologic effect, they produced virus antigens and the infectious virus. Small hybrid cells displayed a more pronounced cytopathologic effect than did big, polynuclear and mitotic cells. Hybrid cells that were passaged 1.5 months after infection did not excrete any infectious poliovirus but contained poliovirus antigens.

Animals↗
PubMed1965

LIPID INCLUSIONS IN L CELLS ASSOCIATED WITH VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS INFECTION.

Hardy, Frank M. (Fort Detrick, Frederick, Md.), and David Arbiter. Lipid inclusions in L cells associated with Venezuelan equine encephalomyelitis virus infection. J. Bacteriol. 89:1101-1103. 1965.-Venezuelan equine encephalomyelitis (VEE) virus has been shown to induce changes of lipid components within the L cell. Lipid inclusions in the form of dark granular bodies were observed in the L cell after aqueous osmium tetroxide fixation and Sudan black staining. Microscopic examination of cells as early as 8 hr after infection with VEE virus showed an increase in the concentration of these inclusions within the cytoplasm. According to various lipid solubility criteria, the inclusions were considered a combination of heterogeneous phospholipids.

Animals↗
PubMed1965

BACTERIOPHAGES THAT LYSE MYCOBACTERIA AND CORYNEBACTERIA, AND SHOW CYTOPATHOGENIC EFFECT ON TISSUE CULTURES OF RENAL CELLS OF CERCOPITHECUS AETHIOPS: A PRELIMINARY COMMUNICATION.

Bacteriophages isolated from sputum and resection specimens of patients suffering from carcinoma of the lung were found to lyse corynebacteria and mycobacteria, and to produce a cytopathogenic effect on certain cells in tissue cultures. From the same and other patients with neoplastic disease, bacteria were isolated and described as coryne-mycobacteria because of bacteriological features they shared with both species. These bacteria, which either were sensitive to mycobacteriophages and corynebacteriophages or were phage-immune lysogenic bacteria, could be induced to produce lytic particles with phagolytic activity on corynebacteria and mycobacteria and a cytopathogenic effect on HeLa cells and on the renal cells of Cercopithecus.

Adenocarcinoma, Bronchiolo-Alveolar↗
PubMed1985

Isolation of a lymphadenopathy-associated virus from a patient with the acquired immune deficiency syndrome.

A virus similar to the lymphadenopathy-associated virus or human T-lymphotropic virus type III, which has been described in association with the acquired immune deficiency syndrome (AIDS) by several laboratories elsewhere in the world, was isolated from a Cape Town patient with lymphadenopathy and acquired immune deficiency. This virus has the characteristic morphogenesis and ultrastructure and its genome encodes the virus-specific p24 protein. It is T-lymphotropic and produces the characteristic cytopathic effect. It can be serially propagated in a human lymphocyte line of the T4+ phenotype. This isolate is being used in diagnostic immunofluorescence assays for virus-specific antibodies.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Propagation of hepatitis A virus in human diploid fibroblast cells.

Human hepatitis A virus (HAV) was propagated in human diploid fibroblast cultures (2BS cells) in vitro. Replication of the virus was followed by immunofluorescent staining (IF), indirect ELISA, and by immune electron microscopy. When 2BS cells were inoculated with faecal extracts containing HAV, synthesis of hepatitis A antigen (HAAg) could be detected in the cytoplasm by IF. Its concentration reached a maximum at four weeks post-inoculation. Measured by solid-phase indirect ELISA, the positive/negative (P/N) ratio for HAAg reached values of up to 7.7. The identity of newly synthesized virus particles with HAV was established by immune electron microscopy, IF-blocking, and neutralization with human convalescent serum. Infected cells showed no signs of a specific cytopathic effect. Two of the virus strains propagated in 2BS cells may prove useful as a source of antigen for serologic tests; one of them might be a candidate strain for HAV vaccine.

Antigens, Viral↗
PubMed1986

Differential response to the cytopathic effects of human T-cell lymphotropic virus type III (HTLV-III) superinfection in T4+ (helper) and T8+ (suppressor) T-cell clones transformed by HTLV-I.

We isolated six human T-cell lymphotropic virus type I (HTLV-I)-transformed T-cell clones carrying the phenotypic markers of helper and suppressor T cells. Five of the transformed T-cell clones produced infectious HTLV-I, but one (clone 55) contained a defective provirus and was therefore not competent for viral replication. To test whether there is interference between HTLV-I and the cytopathic virus HTLV-III in infection and/or their biological effects, we superinfected these T-cell clones with HTLV-III. The recipient cells that we used displayed either the OKT4 or the OKT8 membrane antigens (helper or suppressor phenotype, respectively). The superinfection was successful in all cases, regardless of phenotype of the recipient cells and status of viral production. Both HTLV-III and HTLV-I were expressed by the infected cell lines containing complete HTLV-I proviruses, as demonstrated by electron microscopy and immunofluorescence. However, only HTLV-III in the virus mixture obtained from the culture supernatants was transmitted to the human neoplastic T-cell line H9. The nonproducer clone 55 did not express HTLV-I upon superinfection with HTLV-III. HTLV-III exerted its cytopathic effect on all but one of the superinfected T-cell clones 15-20 days after infection. The exception, clone 67, is also the only cell clone that expresses the phenotypic marker of suppressor T lymphocytes (OKT8); the other clones carry the OKT4 antigen, correlated with helper functions. The virus released from the superinfected clone 67 is cytopathic for fresh peripheral and umbilical-cord blood lymphocytes, suggesting that cellular factors, rather than a genetic change in the virus, may be responsible for the lack of cytopathic effect of HTLV-III on the suppressor T-cell clone 67.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1992

Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation.

We describe the first known occurrence in France of the porcine reproductive and respiratory syndrome (PRRS), a new porcine disease that first appeared in Germany in November 1990. Outbreaks of the disease appeared in November 1991 in Brittany (France), with comparable clinical features to those observed in other countries, were definitively confirmed by a serological analysis from the affected animals using an immunoperoxidase monolayer assay with PRRS-virus infected alveolar macrophages. Furthermore, we report the isolation from one serum of a filtrable agent that, in view of its cytopathic effect for porcine alveolar macrophages and of the serologic reactivity of the infected cells, appears similar to the Lelystad virus that has been implicated in the etiology of the disease.

Animals↗
PubMed1986

Preliminary characterization of a fast-growing strain of human hepatitis A virus.

A fast-growing strain of Human Hepatitis A (HHA) virus was selected by progressively shortening by the time between serial passages of the isolate in the simian cell line Frp/3. The virus so selected infected 90-95% of the cells in 7-10 days, and developed a strong cytopathic effect (CPE). The synthesis of HHA-specific antigens and the CPE were neutralized simultaneously by standard anti-HHA sera. This fast-growing strain has the characteristics of a picornavirus.

Animals↗
PubMedDate not supplied

Replication of infectious bursal disease virus in continuous cell lines.

Three mammalian continuous cell lines--MA-104, Vero, and BGM-70--were tested for their ability to support replication of infectious bursal disease virus (IBDV). Selected tissue-culture-adapted vaccine strains and tissue-culture-adapted field isolates of IBDV replicated in the MA-104, Vero, and BGM-70 cells; cytopathic effects were most pronounced in the BGM-70 cells. The cytopathic effects of the viruses in BGM-70 cells and chicken embryo fibroblast (CEF) cultures were similar. Virus-neutralization titers of selected serum samples determined in BGM-70 cultures compared well with those obtained from CEF cultures.

Animals↗
PubMed1987

Cultivation and characterization of human rotaviruses with "super short" RNA patterns.

Two group A, subgroup I, rotavirus strains possessing "super short" RNA patterns were adapted to growth in MA-104 cells. Both produced marked cytopathic effect in primary culture. Reciprocal cross-neutralization titers, polypeptide analysis, and the serum neutralizing antibody response of an infected child suggested that super short viruses are serotypically distinct from the four recognized human serotypes.

Antibodies, Viral↗