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At least 307 records · Page 17Linked to original sources
PubMed2005

RGD-modified liposomes targeted to activated platelets as a potential vascular drug delivery system.

Local drug delivery has become an important treatment modality for the prevention of thrombotic events following coronary angioplasty. In this study, we investigate the ability of liposomes bearing surface conjugated linear Arg-Gly-Asp (RGD) peptide (GSSSGRGDSPA) moieties to target and bind activated platelets, and the effect of such RGD-modified liposomes on platelet activation and aggregation. The binding of RGD-liposomes to human platelets was assessed by fluorescence microscopy, phase contrast microscopy and flow cytometry. The effect of RGD-modified liposomes on platelet activation and aggregation was investigated in vitro, with and without platelet agonists. RGD-liposomes were found to bind activated platelets at levels significantly greater than the control RGE-liposomes. The RGD-liposomes did not exhibit any statistically significant effect on platelet activation or aggregation. The results demonstrate the ability of the RGD-modified liposomes to target and bind activated platelets without causing significant platelet aggregation and suggests a feasible way for the development of a platelet-targeted anti-thrombogenic drug delivery system. Furthermore, the approach can be extended to the development of liposomes for other vascular targets, for application in drug delivery or gene therapy.

Blood Platelets↗
PubMed1999

[The role of phase contrast microscopy in the evaluation of cervix neck and vaginal biocenosis].

The aim of our study was to evaluate and compare the possibility of germ identification in cervico-vaginal infections of different origin by Ph cytology and classic fixated and stained (H-E) smears. We identified Lactobacillus vag., Gardnerella vag., short bacilli (Escherichia coli, Proteus, Klebsiella), mixed Coccus flora (diplococci, small and large cocci), Trichomonas vag., and Candidia alb. in cervico-vaginal smears. In endocervical, mature metaplastic or parabasal cells the elementary and cocoid bodies of Chlamydia trachomatis were possible to observed. The cytopathic effects caused by viral infections (HPV, HSV, CMV) were detected. The classic (koilocytosis, dyskeratosis) and discrete (binucleation, cracked cells, ghost cells, tadpole cells and large cytoplasmatic hyaline granulations) morphological changes must be confirmed by colposcopy and Digene Hybryde Capture analysis. The results of identification of vaginal biocenosis in Ph microscope and H-E smears were similar. The main benefit of Ph cytology is accuracy, speed and reliability of comprehensive information.

Adolescent↗
PubMed1999

[Integrated colposcopy and phase contrast microscopy for diagnosis of squamous intraepithelial lesions and invasive cervical neoplasms].

Colposcopy or cytology examinations alone allows to obtain diagnostic concordance with histopathological examination between 74-89% and 68-86%, respectively in SIL and cervical cancer detection. Both methods used simultaneously allow increase concordance with histopathology up to 95-100%. Cytological examination may be used simultaneously with colposcopy when Ph microscopy is used. The aim was to estimate benefits of conjunction of colposcopy and Ph cytology in SIL and cervical cancer detection in daily gynecological practice. Integrated colposcopy and cytology examinations were performed in 200 patients. Cervico-vaginal smears were obtained from the patient before Acetic-Acid Test and Lugol-Solution-Test were performed. Wet smears (Ph) were made. Results of integrated colposcopy and cytology were verified by histopathological examination. Diagnostic specificity, sensitivity, and Youden's value were estimated for Ph cytology, H-E cytology, and colposcopy alone and for integrated colposcopy and Ph cytology. Simultaneous colposcopy and Ph cytology reveal specificity 97.1% sensitivity 94.6% and Youden's value 91.8% thus provide fast, reliable and comprehensive information for accurate diagnosis in daily gynecological practice.

Carcinoma, Squamous Cell↗
PubMed2002

[The clinical features and tumor cells characteristics of splenic marginal zone lymphoma].

OBJECTIVE: To deepen the understanding of splenic marginal zone lymphoma (SMZL) and improve the level of diagnosis and therapy. METHODS: A typical case of SMZL, a 61 year old female with lymphocytosis and splenomegaly found fortuitously, was reported. The pathologic, immunologic and genetic features of tumor cells in peripheral blood, bone marrow and spleen were studied with light microscopy, phase contrast microscopy, scanning electron microscopy, immunohistochemical method, flow cytometry, G chromosome banding technique and PCR for studying the pattern of IgH gene rearrangement. RESULTS: The spleen was large with uniform parenchyma and smooth surface. There were multiple small gray-white nodules on sections. Histologically, the neoplastic cells replaced the marginal and mantle zones with complete replacement of germinal centers in the white pulp. The neoplastic cells were predominantly of small to medium size with oval or slightly irregular nuclei. Lymph nodes in the splenic hilum were infiltrated by tumor cells. Immunophenotypic analysis demonstrated that the lymphocytes in the bone marrow expressed CD(20), HLA-DR, CD(45) RA and bcl-2. The monoclonal pattern of IgH gene rearrangement in peripheral blood and bone marrow was found to be the same as that in spleen. After splenectomy, COP chemotherapy and IFNalpha-2a were given and the abnormally increased lymphocytes decreased to normal level. Seven months later the monoclonal rearranged immunoglobulin heavy chain gene pattern changed to polyclonal pattern. CONCLUSION: Splenomegaly, lymphocytosis in peripheral blood and bone marrow without lymph node enlargement and leukocytosis are clinical characters of SMZL. Presence of monoclonal rearranged IgH gene is in favor of the diagnosis. Splenectomy should be done earlier in suspicious patients to avoid malignant transformation.

Antigens, CD20↗