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Phase contrast visualization of squamous cell microridges.

A successful attempt has been made to identify microridges on squamous cells in routine smears using phase contrast microscopy. Microridges may be identified on mature cells of buccal mucosa and in cervical smears, either alcohol or spray fixed, and even in smears up to five years old. The features identified with phase contrast may also be observed with scanning electron microscopy. Identification of microridges may be of diagnostic interest in the evaluation of cervical smears.

Cell Membrane↗
PubMed1978

[Phase contrast microscopy studies of the urine of newborn infants].

Phase contrast microscopy allows a much better identification of the urinary sediment constituents than conventional bright field microscopy. The urinary sediment of 100 neonates admitted in a care unit has been examined by phase contrast microscopy. In all cases, squamous cells are far more numerous in newborn girls than in boys. An important rate of abnormal sediments has been observed: in 40 patients cytologic symptoms of renal ischaemia have been observed (hyaline or granulous casts and renal tubular cells, but red blood cells are uncommon). Numerous uric acid cristals were persent in 22 newborns, in correlation with a prolonged perinatal hypoxia. A considerable bacteriuria was seen in 21 neonates, without concomitant leucocyturia. In conclusion, only 20% of the investigated neonates had a normal urinary sediment.

Bacteriuria↗
PubMed1975

Morphologic and functional characteristics of bone marrow macrophages from imferon-treated mice.

The iron storage macrophage has been isolated from the marrow of Imferon-treated mice and studied in vitro by morphologic, histochemical, and functional tests and isotope labeling techniques. These macrophages on stained preparations are large, many times binucleate cells (up to 150 mu), and show Prussian blue reactivity. In Epon-embedded, stained thick sections they contain elongated narrow basophilic inclusions. These macrophages are actively phagocytic and pinocytic; histochemical studies show that these cells are rich in acid phosphatase, nonspecific esterase, and PAS diastase-resistant activity. Iron storage macrophages do not incorporate the 3H-thymidine. The electron microscopic appearance of this macrophage shows that the cell has ferritin free in the cytoplasm and several types of cytoplasmic granules: those with large quantities of electron-dense ferritin and/or hemosiderin (type A), elongated granules (type B) with moderately electron dense homogeneous matrix and some ferritin at the periphery, and granules with heterogeneous content (type C). The above findings demonstrate that the iron storage cell is a mature macrophage which contains hydrolases, ferritin, and a unique population of cytoplasmic granules which are lysosomal in nature. There is some evidence to suggest that the unusual lysosome (type B granule) occurs after macrophages have ingested erythrocytes.

Acid Phosphatase↗
PubMed1975

Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes.

A group of alloantibodies are found in pregnancy sera which react with antigens present on B lymphocytes and monocytes but are not detectable on the vast majority of unstimulated T cells. This specificity distinguishes them from HL-A antibodies which react with both cell types. They were readily recognized through indirect fluorescent antibody analysis by employing the combination of B-cell lymphoid lines and normal peripheral blood T cells. Different sera gave a variety of patterns of reactivity with a panel of 11 lymphoid lines. Similar differential patterns were also observed with normal B cells from different individuals particularly after concentrating the B cells. The antibodies were also cytotoxic to B cells and this procedure gave parallel results to the fluorescence method. The pattern of reactions obtained indicated a very heterogeneous system similar to that for HL-A. Special study of certain of the sera provided evidence that the lymphocyte-defined determinants of the mixed lymphocyte reaction system were involved. For convenience the term HL-B has been employed for these antigens.

Antibody Specificity↗
PubMed1975

[Demonstration of immunoglobulin determinants on lymphocyte surfaces by membrane immunofluorescence].

Isolated lymphocytes from peripheral blood of 22 healthy donors were examined with the help of the indirect membrane immunofluorescence (MIF) for immunoglobulin determinants on the surface of the cells. The quantity of MIF+-cells for IgM was on the average 8.7% (3-18) and for IgG 15.5%(8-18). Five different anti-IgM-sera were characterised with regard to their use in the MIF (precipitation titre, entire protein content, content of anti-IgM-antibodies). Differences in the quantity of the MIF+-lymphocytes were above all based on differing antibody concentrations of the sera. It was referred to further possible causes for these differences. The influence on the results of the examinations by protein addition and trypsination.

Adult↗
PubMed1975

[Cytotoxic effects of a leukocidin from Staphylococcus aureus (author's transl)].

In an attempt to improve the assay of leukocidin from Staphylococcus aureus a combined vital fluorochrome staining procedure with acridine organe and ethidium bromide was developed. It proved to be more suitable for the demonstration of leukocidic effects on leukocytes than phasecontrastmicroscopy. Damaged leukocytes fluoresced distinctly red and undamaged leukocytes green. With the fluorochrome-method leukocidic changes became also evident in some lymphocytes. These were not clearly demonstrated by phasecontrastmicroscopy.

Acridines↗