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At least 307 records · Page 17Linked to original sources
PubMed1983

Murine type C retroviruses and intracisternal A-particles in human tumors serially passaged in nude mice.

An ultrastructural survey of 11 human tumors passaged in N:NIH(S) (nu/nu) mice showed two instances of type C virus production. In one instance type C virus particles were observed in the endothelial murine stromal cell component of an embryonal carcinoma but not in the human tumor cells. In another instance type C virus particles were seen replicating in the chondroblastic human cells of a xenografted osteosarcoma. The type C virus produced in the human cells failed to transform NIH/3T3 cells, the C-127 rat cell line, or mink cells. Nucleic acid hybridization studies in which a human endogenous retroviral probe and a xenotropic murine leukemia virus envelope probe were used suggested that the retrovirus present in the human osteosarcoma cells is related to murine leukemia viruses. Intracisternal A-particles (IAP) were also detected in the human osteosarcoma cells. Their presence in the human cells was demonstrated by simultaneous visualization of IAP and human HLA determinants at the cell surface. The literature on type C virus infection of human cells and tumors grafted in nude mice is reviewed.

Animals↗
PubMedDate not supplied

The effect of ceruloplasmin on the multiplication and on some biological and physico-chemical characteristics of influenza virus A/PR8/34 (H0N1) cultivated in chorioallantoic membrane fragments. Note 2. Modifications occurring in the course of serial passages.

The results obtained at the first passage of ceruloplasmin-incubated influenza virus A/PR8/34 (H0N1) in chorioallantoic membrane fragments demonstrate the "trap effect" of ceruloplasmin and are characterized by the low values of the ratios between the hemagglutinating and neuraminidase activities and the infectivity of the resulted progens. At the 2nd passage, both in the absence and in the presence of ceruloplasmin the previously observed changes are accompanied by severe modifications in the antigenicity of the progens, as shown by the HAI titres obtained against a rabbit antiserum to influenza virus A/PR8/34 (H0N1).

Allantois↗
PubMed1980

Effect of serial passages through liquid medium on the virulence of Mycoplasma bovis for the mouse mammary gland.

The mouse mastitis model was used to examine strains of Mycoplasma bovis. Strains that had been passaged in liquid medium more than 60 times were markedly less virulent than the same or different strains with fewer passages. Whereas the low passage strains produced a systemic response in some mice and severe pathological and histopathological changes in the mammary glands of all, the high passage strains produced only minor histopathological changes.

Animals↗