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Glaucopines A and B, new cyathane diterpenes from the fruiting bodies of Sarcodon glaucopus.

Glaucopine A (1) and B (2), two new cyathane diterpenes, have been isolated from the mushroom Sarcodon glaucopus: their structures have been determined on the basis of spectral data. Their topical anti-inflammatory activity was evaluated using the Croton oil ear test in mice: compounds 1 and 2 (1 micromol/cm2) provoked edema reduction (62 % and 55 %, respectively) similar to that induced by the reference non-steroidal anti-inflammatory drug, indomethacin (0.3 micromol/cm2, 61 % edema reduction).

Animals↗

A novel regulation on developmental gene expression of fruiting body formation in Myxobacteria.

Myxobacteria are Gram-negative soil microorganisms that prey on other microorganisms. Myxobacteria have significant potential for applications in biotechnology because of their extraordinary ability to produce natural products such as secondary metabolites. Myxobacteria also stand out as model organisms for the study of cell-cell interactions and multicellular development during their complex life cycle. Cellular morphogenesis during multicellular development in myxobacteria is very similar to that in the eukaryotic soil amoebae. Recent studies have started uncovering molecular mechanisms directing the myxobacterial life cycle. We describe recent studies on signal transduction and gene expression during multicellular development in the myxobacterium Myxococcus xanthus. We provide our current model for signal transduction pathways mediated by a two-component His-Asp phosphorelay system and a Ser/Thr kinase cascade.

Gene Expression Regulation, Bacterial↗

Concentrations of mercury, copper, cadmium and lead in fruiting bodies of edible mushrooms in the vicinity of a mercury smelter and a copper smelter.

Four elements were determined in wild growing mushrooms collected in the vicinity of two metal smelters up to a distance of 6 km between 1990 and 1993. The smelters in eastern Slovakia are 15 km apart. Elements were determined by atomic absorption spectrometry in 113 samples of 34 species. The values observed in mushrooms from different parts of Bohemia were used as the background levels. Concentrations of mercury were extremely high in mushrooms grown in the mercury smelter area, especially those in Lepiota procera with mean and maximal values of 119 and 200 mg kg-1 dry matter, respectively. Considerably elevated mercury concentrations were also found in the copper smelter area, especially in Lepista nuda with a mean value 84.7 mg kg-1 dry matter. In both species there were also increased copper concentrations exceeding 200 mg kg-1 dry matter and in the copper smelter area there were high lead values in Lepiota procera and Lepista nuda at 26.4 and 15.3 mg kg-1 dry matter, respectively. Mean cadmium concentrations were also increased in the copper smelter area, but with values < 7 mg kg-1 dry matter. Thus, the mushrooms from both areas should not be consumed at all.

Basidiomycota↗

Assays of the biological activities of guaiane sesquiterpenoids isolated from the fruit bodies of edible lactarius species.

Three sesquiterpenoids that are found in the edible mushrooms Lactarius deliciosus, L. deterrimus and L. sanguifluus, have been assayed for biological activity. The compounds tested were a stearic acid ester of a sesquiterpene (I) and a sesquiterpene aldehyde (lactaroviolin, II) and alcohol (deterrol, III). The assays used were for mutagenic activity in the Ames Salmonella assay, for antimicrobial activity against bacterial fungi and algae, for cytotoxicity against Ehrlich ascitic tumour cells and L 1210 cells, and for phytotoxic activity against Lepidium sativum and Seteria italica. All three compounds showed weak mutagenic activity in the Ames assay. Two compounds (II and III) were found to have moderate cytotoxic activity and one (III) exhibited weak antibacterial activity. No compound revealed phytotoxic, algicidal or antifungal activity.

Animals↗

Adustin, a small translation-inhibiting polypeptide from fruiting bodies of the wild mushroom Polyporus adusta.

A polypeptide, with a molecular mass of 16.5 kDa as determined by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has been isolated from the mushroom Polyporus adusta. The polypeptide, designated as adustin, inhibited translation in a cell-free rabbit reticulocyte lysate system with an IC50 of 0.34 microM. It was isolated using a protocol that involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-Sepharose and fast protein liquid chromatography-gel filtration on Superdex 75. Adustin was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and CM-Sepharose.

Animals↗

Isolation of trichogin, an antifungal protein from fresh fruiting bodies of the edible mushroom Tricholoma giganteum.

An antifungal protein was isolated from the mushroom Tricholoma giganteum var. golden blessings. The protocol included ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The antifungal protein, designated trichogin, was unadsorbed on DEAE-cellulose but was adsorbed on Affi-gel blue gel and CM-cellulose. It exhibited antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. Trichogin inhibited HIV-1 reverse transcriptase with an IC50 of 83 nM.

Agaricales↗