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At least 325 records · Page 18Linked to original sources
PubMed1977

Human lymphocyte culture: intrinsic factors influencing the quality of chromosome preparations.

In a cytogenetic study of the consecutive cell cycles (M1, M2 etc.) from human lymphocyte cultures after 5-bromodeoxyuridine incorporation followed by differential Giemsa staining, the metaphases of M2 and subsequent in vitro divisions have been found to spread better than those of M1 cells, as if the cells of in vitro generation suffer some membrane alterations. Moreover presence of S or G2 phase, leukocytes in a blood sample may result into early proliferation in a PHA stimulated culture system, so that the metaphase yield at 72 h will be larger than otherwise, a sizeable portion of which, being in M2 or subsequent cell cycles, will spread better. Thus such individual or transient intrinsic factors of haematologic state may influence the success of a leukocyte culture and the quality of chromosome preparation.

Adult↗
PubMed2000

Role of pHyde novel gene product as an intrinsic factor for apoptotic pathway in prostate cancer.

Induction of apoptotic cell death mechanism can be regulated by internal factor(s), such as by gene product(s) that directly upregulate the apoptosis pathway or indirectly by down-regulating the anti-apoptosis gene. This homeostasis is a normal phenomenon in a biological system disturbed by cancer. It is thus important to find any gene functioning as an upregulator for the apoptosis pathway that may have a potential application in the context of cancer gene therapy. We have cloned a novel rat gene, denoted as pHyde, that fulfilled this objective. Internally, this pHyde gene product renders the stable transfectant of rat prostatic cancer cell lines more susceptible to apoptosis even without any external inducer. By using an external agent, such as 5-fluoro-2'-deoxyuridine (FdUr), apoptotic responses of the stable transfectants are even higher, suggesting that both intrinsic and extrinsic factors work synergistically. The pHyde gene product was termed an intrinsic factor, whereas FdUr was considered an extrinsic factor for the apoptosis in rat prostate cancer model.

Animals↗
PubMed1981

Solubilization of the ileal receptor for intrinsic factor--vitamin B-12 complex by digestion with papain.

Brush-border-membrane vesicles isolated from hamster ileum were incubated with either papain or Pronase P and subsequently centrifuged to obtain soluble (supernatant) and insoluble (pellet) fractions. Papain (4 units/ml) solubilized 95--100% of the sucrase and leucine naphthylamide-hydrolysing activities but only 30% of the alkaline phosphatase. Digestion with papain also resulted in the solubilization of more than 75% of the ileal receptor for intrinsic factor-vitamin B-12 complex with a corresponding decrease in receptor activity in the pellet. Essentially 100% of the receptor activity was recovered. In contrast, digestion with Pronase P resulted in a decrease in total receptor activity. Papain-solubilized receptor was not sedimented by centrifugation at 105 000 g for 90 min and was eluted in the included volume of Sepharose 6B. Like the binding to more intact preparations, binding of intrinsic factor-vitamin B-12 complex to papain-solubilized receptor was rapid, reaching 50% of maximum in 8 min, and required Ca2+. Although Mg2+ could not completely substitute for Ca2+, Mg2+ did stimulate Ca2+-dependent binding at low Ca2+ concentrations. These results demonstrate that the ileal receptor for intrinsic factor-vitamin B-12 complex can be solubilized with papain, and suggest that papain solubilization may be a useful first step in the isolation and purification of this receptor.

Animals↗