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At least 325 records · Page 18Linked to original sources
PubMed1984

A new immunologic test for CNS cysticercosis.

A new radioimmunoassay for cerebral cysticercosis was studied in 70 patients. The assay showed nearly 100% sensitivity for ventricular cysts or meningitis, 86% sensitivity for multiple parenchymal cysts, and a false-positive rate of 7%. Both serum and CSF antibody levels were useful diagnostically, and the contribution of both improved accuracy. In some patients, there was endogenous CNS production of IgG against the cysticercus antigen, which leads to elevated CSF levels and normal serum levels. Patients with high CSF total IgG levels may show false-positive CSF antibody elevation with normal serum levels.

Antibodies↗
PubMedDate not supplied

[Quality control of immunologic testing of blood in the Region of the Americas].

Assuring the best quality in transfusion medicine necessarily involves exercising exhaustive control over the entire system and developing protocols for procedures and techniques, the reagents and equipment used, personnel training, and so on. Additional guarantees can come from adequately organizing the work and perhaps automating processes in order to avoid errors. Another way to avoid errors in the laboratory is to register and continuously analyze the mistakes that do occur, since that makes it necessary to change procedures in order to avoid repeating them. External quality control programs (EQCP) make it possible to perform a periodic overall assessment of the suitability of techniques, reagents, and training of personnel in relation to the validity of the results obtained. By voluntarily participating in an EQCP, the blood center shows its commitment to the quality of care. In the Region of the Americas, the Pan American Health Organization's Immunohematology Quality Control Program has made it possible to objectively quantify the improvement attained in the formative level reached by participating blood banks in the four years the Program has been in operation.

Blood Banks↗
PubMed1978

[Immunological Tests By Anti-Free-Living Amoebas Serum Produced In Experimental Animals: I. Immobilization Of Free-Living Amoebas In Vitro By Rabbit Antiserum]

Rabbits were immunized with free-living amoebas by intravenous injections. The amoebas were Acanthamoeba culbertsoni and Naegleria fowleri and obtained by axenic cultivation in CGVS medium. Each rabbit received 10(6) of Acanthamoeba culbertsoni and 10(5) of Naegleria fowleri trophozoites respectively every other day in three doses and finally one booster dose at 1 week later. Antiserum was collected from thc following day of the booster injection up to 2 months period, and stored at -30 degrees C until use. The immobilization test was performed. One drop of amoeba suspension was mixed with the test serum on slide and observed the mobile state under microscope. 1. Maximal immobilizing phenomenon observed in 30 minutes and, then gradually recovered to normal state. 2. Inactivation of antiserum at 56 degrees C for 30 minutes did not affect the immobilization phenomenon. 3. The immobilization rates decreased by the serial dilution of antiserum. At dilution more than 1:8, the immobilization was almost the same as in the normal serum. 4. The immobilizing antibody in anti-Acanthamoeba culbertsoni rabbit serum showed highest titre in 3rd day after booster immunization and from first to 6th week in anti-Naegleria fowleri rabbit serum. 5. Cross matching of Acanthamoeba culbertsoni and Naegleria fowleri showed antigenic difference of the two species. It is suggested that the immobilization reaction may be of value as a supplementary test in the diagnosis of primary amoebic meningoencephalitis.

Journal Article↗
PubMed1978

[Immunological Tests By Anti-Free-Living Amoebas Serum Produced In Experimental Animals: II. Indirect Fluorescent Antibody Titer Of Anti-free-living Amoebas Serum Produced In Rabbits]

The indirect fluorescent antibody test was performed to demonstrate the antibody production in the rabbits immunized with free-living amoebas; Acanthamoeba culbertsoni and Naegleria fowleri, and antibody titer changes by immunization duration. Rabbits were immunized with Acanthamoeba culbertsoni and Naegleria fowleri which were cultured axenically in CGVS medium. For experiments, rabbits were divided into two groups; small dose group received 10(4) intravenously with live or dead free-living amoebas trophozoites as an immunizing dose three times with one week interval, and large dose group received 10(6) live or dead trophozoites respectively. The control group received physiologic saline or medium for culture of free-living amoebas intravenously. Antiserum was collected 4 times at interval of 3 days in the first 10 days, and also up to 2 months later. In the group immunized with live Acanthamoeba culbertsoni, fluorescent antibody titer was higher than in the group of dead one, and also in the large dose group than in the small dose group. Antibody titer of anti-Naegleria fowleri serum in the large dose group showed no difference by the source of amoeba antigen; live or dead. But in the small dose group, antibody titer was higher in the immunized with live Naegleria fowleri than in the group with dead one. No cross reactivity was demonstrated between the Acanthamoeba and Naegleria. And no cross reaction was observed when the free-living amoebas antigens were tested against human sera of amoebiasis, paragonimiasis and clonorchiasis.

Journal Article↗
PubMed1979

Investigation of a valuable biochemical indicator in radiotherapy. I. Immunological tests and reticulocyte number.

Patients with different cancers were treated by 200-kV X-rays, high-energy X-rays and 60Co-gamma rays. Changes in the immunoglobulin level were observed by immunoelectrophoresis qualitatively and by immunodiffusion quantitatively. Reticulocyte count was made in smears. It was concluded that immunoelectrophoresis was only of limited value in the investigation of a biochemical indicator in radiotherapy; immunodiffusion was more promising. Slight immunosuppressive tendency and return to the control level was observed during treatment. An increase in reticulocyte number during radiotherapy seems to be a good prospect in evaluating the effectivity of the treatment.

Cobalt Radioisotopes↗
PubMed1964

A SIMPLE IMMUNOLOGIC TEST FOR PREGNANCY.

Experience with a latex agglutination-inhibition slide test for human chorionic gonadotrophin which allows pregnancy to be diagnosed in three minutes is described. Among 350 tests performed, 115 were in patients who presented with some complication of a presumed pregnancy, and 132 patients presented diagnostic problems in which pregnancy was to be ruled out. Clinical follow-up confirmed the accuracy of the test and its usefulness in the management of such clinical problems. In addition, among 48 patients with normal pregnancy between 42 and 120 days in duration, the only false-negative test was in one patient who was exactly 42 days from her last normal menstrual period.

Body Fluids↗