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PubMed2000

[Fluorescence in situ hybridization applied to the meiotic analysis and spontaneous chromosome translocation in the pollen mother cells of hybrids of Triticum-Haynaldia].

Fluorescence in situ hybridization was applied to the meiotic analysis and chromosome pairing in the pollen mother cells of hybrids of Triticum-Haynaldia. The results indicated that most (90.5%-93.2%) of the PMCs of two Triticum-Haynaldia hybrids contained two univalents involving one Haynaldia chromosome at metaphase I, and only 1 of 320 PMCs with homologous pairing between one wheat chromosome and one Haynaldia chromosome was observed in the hybrid of Guinong 22 x common wheat Chinese Spring, the frequency was 0.3%. The frequencies of the PMCs with mis-dividing chromosome ranged from 32.7% to 37.5% at anaphase I and from 20.5% to 24.4% at anaphase II in the hybrids between Triticum-Haynaldia. The frequency of Haynaldia chromatin translocating into Triticum chromosome was about 0.7% at anaphase I in the hybrid of Guinong 22 x common wheat Chinese Spring and was 0.8%-1.7% at anaphase II in two wheat-Haynaldia hybrids. Otherwise, the chromosome morphology involving Haynaldia chromatin was observed at preleptotene I, and the frequencies of chromatin nucleus at tetrad stage and at microspore stage were investigated. The time of chromosome translocation in meiosis and the transmitting frequencies of Haynaldia chromosome in wheat background were discussed.

Gene Frequency↗

PubMedDate not supplied

[Comparative characteristics of reproduction of the wheat striped mosaic virus in winter and spring Triticum aestivum L. in natural agrocoenosis and during clinostatting].

Microgravity (a transformed environment) was produced with the use of a multi-purpose clinostat. Object of the investigation was wheat striped mosaic virus (WSMV) affecting a great variety of wheat species in natural agrocoenosis, and super-dwarf cultivar Apogee in the transformed environment. Enzyme immunodetection (das-ELISA) as well as electron microscopy were employed for virus identification. Viral reproduction was found high (titre 1/2560) in winter and spring wheat species in agrocoenosis (natural transmissible background). Clinostatting in the horizontal and vertical planes (R = 400 mm and R = 250 mm) inhibited virus reproduction by day 21, 25 and 18 post inoculation, respectively. Clinostatting improved health of WSMV-infected plants and resulted in harvesting the first Apogee crop. Productivity of WSMV-infected plants after clinostatting was increased owing to a large seed content of the heads as compared with stationary and clinostatted healthy crops. This experience of inhibiting virus reproduction should be taken into consideration in virus-free seed production biotechnology development.

Animals↗

PubMed1996

cDNA encoding a wheat (Triticum aestivum cv. Chinese spring) glycine-rich RNA-binding protein.

A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings, whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.

Abscisic Acid↗

PubMed1995

Interaction of maternal photoperiod history and food type on growth and reproductive development of laboratory meadow voles (Microtus pennsylvanicus).

The interaction of maternal photoperiod history and four diets were tested by measuring body growth, reproductive development, and pelage development in 9-week-old juvenile meadow voles. Meadow vole dams were housed in long daylengths (LD; 14 h light/day), short daylengths for 2 weeks (SD; 10 h light/day), or short daylengths for 26 weeks (PR; photorefractory) prior to mating. Immediately following parturition, one of four diets was available to dams and pups; (a) a control diet containing no 6-methoxy-2-benzoxazolinone (6-MBOA); (b) the control diet plus sprouted wheat (which contains 6-MBOA); (c) the control diet plus alfalfa harvested in spring (no 6-MBOA); and (d) the control diet plus alfalfa harvested in autumn (no 6-MBOA). By 9 weeks of age, juvenile meadow voles born to photorefractory dams and fed either spring or fall alfalfa or sprouted wheat were significantly larger and more had achieved puberty than juveniles fed only the control diet. Juveniles born to LD dams demonstrated a smaller increase in developmental rate than photorefractory juveniles when fed alfalfa and spring wheat, and juveniles of SD dams showed the smallest effect of alfalfa and sprouted wheat on development. Supplements of spring wheat and both forms of alfalfa had similar positive effects on growth and reproduction. The authors suggest that juvenile meadow voles rely on the interaction of maternal photoperiod history and the availability of nutrient-rich food such as sprouted wheat and alfalfa to time the onset of growth and puberty.

Animals↗

PubMed2004

An interspersed refuge for Sitodiplosis mosellana (Diptera: Cecidomyiidae) and a biocontrol agent Macroglenes penetrans (Hymenoptera: Pteromalidae) to manage crop resistance in wheat.

An interspersed refuge of susceptible plants in a resistant, spring-sown wheat crop was tested as a strategy to protect crop resistance against evolution of virulence by the wheat midge Sitodiplosis mosellana (Géhin), and also to conserve a biocontrol agent Macroglenes penetrans(Kirby). Eight replicated field experiments were conducted using seed mixtures of 0, 5, 10, 15 and 100% or 0, 5 and 100% susceptible wheat with an agronomically similar wheat expressing the antibiotic resistance gene Sm1. The frequencies of eggs, mature larvae and parasitized larvae in susceptible and resistant wheat spikes, and midge-affected seeds in the harvest, were recorded for each plot. In susceptible wheat, insect densities and seed damage were typical of those in commercial wheat. In resistant wheat, few larvae completed development, 2% or less compared with about 80% in susceptible wheat, when larvae were sampled at maturity. This resistant wheat also deterred midge oviposition, reducing egg densities by 65% compared with susceptible wheat. The wheat midge and its parasitoid oviposited throughout the plots, and parasitism was density independent. The densities of mature midge larvae and parasitoids were in proportion to the size of the refuge. A 5% susceptible refuge produced about 41 mature larvae for each mature larva from the resistant wheat, and provided effective control of damage. An interspersed refuge of susceptible plants in resistant wheat is a promising strategy for sustaining resistance conferred by Sm1 and biocontrol of the wheat midge.

Animals↗

PubMed1985

Insertion of eukaryotic DNA into the Bacillus subtilis genome by means of a temperature-sensitive plasmid vector.

A hybrid temperature-sensitive plasmid capable of integration into the Bacillus subtilis genome was constructed. By using this vector, we inserted a 3.2-kb fragment of eukaryotic DNA (wheat 'Chinese Spring') into the bacterial genome. The fragment of wheat DNA was stably retained and replicated as a part of the bacterial genome. The position of the integrated plasmid in the B. subtilis genome was mapped, as was the site in wheat DNA insert on plasmid at which the integration occurred.

Bacillus subtilis↗

PubMed2002

Chromosomal location of 46 new RAPD markers in rye (Secale cereale L.).

The polymerase chain reaction (PCR) was used to locate RAPD markers using disomic wheat-rye addition lines in order to develop a set of molecular markers distributed on the seven rye chromosomes. We carried out RAPD amplifications on genomic DNA of wheat 'Chinese Spring' (CS), rye 'Imperial' (I), the amphiploid wheat-rye and the seven disomic wheat-rye addition lines (1R-7R) using 140 different 10-mer oligonucleotides. Forty six new RAPD markers were located on the seven rye chromosomes and all the disomic wheat-rye addition lines were identified on the basis of their amplification patterns. The number of RAPD bands located on 1R, 2R, 3R, 4R, 5R, 6R and 7R chromosomes were 5, 8, 11, 8, 8, 10 and 6, respectively. The seven wheat-rye addition lines can be distinguished using only the following three 10-mer oligonucleotides: OPA16, OPF19 and GEN3-605, the other RAPD primers being useful for this purpose. The use of these RAPDs as a source of molecular markers that could be linked to interesting genes or other important agronomic traits is discussed.

Chromosome Mapping↗

PubMed2004

Inheritance of the light intensity response in spring cultivars of common wheat.

The effects of low/high light intensities and day length on ear emergence time in climatic chambers were studied in 12 common wheat (Triticum aestivum L.) cultivars of different ecogeographical origin. Low light intensity (LI) affected the time to ear emergence in all the wheat cultivars of both the photoperiod sensitive and insensitive genotypes, increasing the number of days to ear emergence (DEE). Based on the increase in DEE, we chose samples with different light intensity responses among the cultivars and analyzed their F2 hybrids to see if they were segregating. Taken together, the data for the F2 plants and test cross showed that the strong response to light intensity is a recessive trait and that the parental cultivars differ by the two genes controlling the LI response in common wheat. Besides heading time, low LI increased the number of days to tillering in all the cultivars except Pitic 62, but short day affected the period to tillering less than low LI. The symbol Rli (the response to light intensity) is suggested to designate the genetic control of the response to LI in wheat. Thus, the response to LI may influence the adaptability to changing environmental conditions and yield of wheat cultivars.

Genes, Plant↗

PubMed2000

[Identification of the barley chromosome in wheat by GISH and RFLP].

The barley chromosome in wheat was identified by genomic in situ hybridization (GISH) in which biotin labelled total genomic DNA of barley Betzes was used as probe and the unlabelled total DNA of common wheat Chinese Spring (CS) as blocking DNA. A series of wheat materials were tested as follows: two disomic alien substitution and monosomic alien addition lines, 2n = 43; two monosomic alien substitution lines, 2n = 42; seven disomic alien substitution lines, 2n = 42. RFLP probe psr131 on the short arm of the homologous group 2 was used to analyze the barley chromosome in wheat. The result indicated that there was a same band in barley Betzes and substitution line A5. The chromosome 2A of A5 was substituted by the chromosome 2H of barley. These materials will be useful in transferring the valuable genes in the chromosome 2H to wheat.

Chromosomes↗

PubMed2005

Comparative biology of a novel strain of Russian wheat aphid (Homoptera: Aphididae) on three wheat cultivars.

In spring 2003, several outbreaks of the Russian wheat aphid, Diuraphis noxia (Mordvilko), were reported in fields of supposedly resistant wheat cultivars ('Stanton', 'Halt', and 'Prairie Red') in eastern Colorado. We conducted two laboratory experiments to compare the biological performance of this new biotype 2 (B2) to that of two D. noxia collections of biotype 1 (B1) from western Kansas by using three wheat cultivars as host plants: 'Trego', a susceptible cultivar, and Stanton and Halt, two cultivars with different genetic sources of resistance. Survival of solitary nymphs from first instar to adult for the two clones of B1 on Trego was 96 and 90%, respectively, compared with 67 and 43% on Stanton, and 65 and 57% on Halt. In contrast, B2 had 60% survival on Trego, 43% survival on Halt, and 85% survival on Stanton. One clone of B1 required longer to mature on Halt compared with Trego or Stanton, but no other differences in developmental time among cultivars were significant. The standardized fecundity of solitary foundresses of the B1 clones was 19.6 and 20.1 nymphs on Trego, compared with 4.6 and 0.9 on Stanton, and 2.8 and 1.1 on Halt, respectively, over the same period. In contrast, fecundity of B2 was 21.1, 20.8, and 19.7 on Trego, Stanton, and Halt, respectively. When larger colonies developed on individual plants over longer periods, Trego supported the largest number of B1 aphids by experiment's end, whereas Stanton and Halt yielded the largest numbers of B2. The order of overall plant damage was Trego > Stanton > Halt when infested with B1, with no significant differences for B2. Trego had more pronounced leaf rolling than other cultivars, independent of biotype. Collectively, the results suggest that D. noxia B2 from Colorado has evolved cross-virulence to both Dn4- and Dny-based resistance sources.

Animals↗

PubMedDate not supplied

Production of near-isogenic lines and marked monosomic lines in common wheat (Triticum aestivum) cv. Chinese Spring.

Sixteen near-isogenic lines (NILs) carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of common wheat (Triticum aestivum) cv. Chinese Spring (CS). Three genes from alien species showed segregation distortion. In NILs carrying a marker gene of rye (Secale cereale) or Aegilops caudata, the alien chromosome segments were detected by fluorescence in situ hybridization (FISH). The NILs were grown with replications and the effect of marker genes on plant morphology in the genetic background of CS was investigated. These NILs were further crossed with the corresponding monosomics of CS and 13 monosomic lines whose monosome carries a respective marker gene were established and named "marked monosomics." Many of the marked monosomics were distinguishable from the disomic NILs because of the different dosage effect of the genes. The NILs are utilized for studies on gene isolation or gene regulation. Marked monosomics are useful not only for monosomic analysis but also for production of homologous chromosome substitution lines because chromosome observation is not required.

Alleles↗

PubMed2005

Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat.

The broad adaptability of wheat and barley is in part attributable to their flexible growth habit, in that spring forms have recurrently evolved from the ancestral winter growth habit. In diploid wheat and barley growth habit is determined by allelic variation at the VRN-1 and/or VRN-2 loci, whereas in the polyploid wheat species it is determined primarily by allelic variation at VRN-1. Dominant Vrn-A1 alleles for spring growth habit are frequently associated with mutations in the promoter region in diploid wheat and in the A genome of common wheat. However, several dominant Vrn-A1, Vrn-B1, Vrn-D1 (common wheat) and Vrn-H1 (barley) alleles show no polymorphisms in the promoter region relative to their respective recessive alleles. In this study, we sequenced the complete VRN-1 gene from these accessions and found that all of them have large deletions within the first intron, which overlap in a 4-kb region. Furthermore, a 2.8-kb segment within the 4-kb region showed high sequence conservation among the different recessive alleles. PCR markers for these deletions showed that similar deletions were present in all the accessions with known Vrn-B1 and Vrn-D1 alleles, and in 51 hexaploid spring wheat accessions previously shown to have no polymorphisms in the VRN-A1 promoter region. Twenty-four tetraploid wheat accessions had a similar deletion in VRN-A1 intron 1. We hypothesize that the 2.8-kb conserved region includes regulatory elements important for the vernalization requirement. Epistatic interactions between VRN-H2 and the VRN-H1 allele with the intron 1 deletion suggest that the deleted region may include a recognition site for the flowering repression mediated by the product of the VRN-H2 gene of barley.

Alleles↗

PubMed1988

The transcription and translation in vitro of individual cereal storage-protein genes from wheat (Triticum aestivum, cv. Chinese Spring). Evidence for translocation of the translation products and disulphide-bond formation.

Genes coding for the high-Mr ['high-molecular-weight' (HMW)] glutenin subunit 12 and for a gamma-gliadin from wheat (Triticum aestivum, cv. Chinese Spring) were subcloned into transcription-translation vectors. In each case transcription in vitro yielded a RNA transcript which when added to a rabbit reticulocyte cell-free translation system directed the synthesis of a polypeptide of appropriate Mr by SDS/polyacrylamide-gel electrophoresis (SDS/PAGE). When dog pancreatic microsomal vesicles were added to the translation system, translocation of the newly synthesized polypeptides occurred, as judged by protection from proteolysis. When translation and translocation of the gamma-gliadin was carried out under conditions favouring the formation of disulphide bonds, a polypeptide was synthesized which had a faster mobility on SDS/PAGE carried out under non-reducing conditions than under reducing conditions. This suggests that the processed and translocated gamma-gliadin forms an intramolecular disulphide bond or bonds during synthesis in vitro.

Cell-Free System↗

PubMed2003

[Analysis of the effects of parental genotypes of rye lines on the development of quantitative traits in primary octaploid triticale. Plant height].

When breeding the primary spring octoploid triticale derived from crosses of various inbred rye lines to wheat Chinese Spring, the effects of the rye genotype and growth conditions on the plant height and proportion of the first, second, and final (pedicle) internodes to the entire stem length were studied. Two triticale groups were examined: homozygotes for the dominant (Ddw1) and recessive (ddw1) alleles of the gene responsible for short stem in rye. In the short stem triticale lines carrying the Ddw1 alleles, the plants were 20 cm shorter on average than those in the ddw1-carrying lines, and the distribution of the two triticale groups overlapped significantly. In both groups, the lines significantly differing in plant height could be differentiated, because of allelic diversity of the additional genes controlling this trait along with the Ddw gene. In most triticale lines, especially in the Ddw1-carrying ones, the plant height was much reduced under unfavorable growth conditions. At the same time, a short-stem line was isolated, which is characterized by ecological plasticity, like the maternal wheat cultivar. In the triticale studied, the stem structure depended on the short-stem rye genotype. The two-year study showed that in the triticale carrying the dominant allele of this gene, the first internode is significantly extended, whereas the upper (pedicle) internode is reduced, which increases plant lodging resistance. The differences revealed between the rye lines as well as their effect on the quantitative triticale traits are discussed in view of a variant of the hybridological analysis, which had been previously proposed for identification and mapping of the correspondent rye genes.

Alleles↗

PubMedDate not supplied

[Inheritance of heading date in crosses of wheat cultivar responsiveness to light intensity].

The inheritance of heading date has been studied in crosses of spring bread wheat cultivars differing in their reaction to various light intensities. The parental cultivars were similar in their Vrn and Ppd gene systems and differed in heading dates. Domination of higher reaction to light intensity has been shown in F1 on duration of "ear emergence-heading" period. Analysis of F2 populations has shown the identity of earliness per se genetic systems in Novosibirskaya 22 and Ordynskaya cultivars. Digenic differences have been revealed for both these cultivars from Tyumenskaya rannyaya cultivar on duration of "ear emergence--heading" period.

Crosses, Genetic↗

PubMed1998

Molecular cytogenetic characterization of Thinopyrum and wheat-Thinopyrum translocated chromosomes in a wheat-Thinopyrum amphiploid.

The wheat-Thinopyrum amphiploid 'Agrotriticum # 3425' (AT 3425), which is highly resistant to Cephalosporium stripe, was identified to carry seven pairs of Thinopyrum chromosomes, three pairs of wheat-Thinopyrum translocated chromosomes and 18 pairs of wheat chromosomes. Fluorescence genomic in situ hybridization (FGISH), C-banding, sequential C-banding and FGISH, and denaturing polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize and identify the chromosomes. The Thinopyrum chromosomes in AT 3425 were designated as T1 through T7 based on their C-banding patterns. The FGISH and C-banding patterns of mitotic chromosomes in AT 3425 and meiotic chromosomes in the hybrid between AT 3425 and wheat cultivar 'Chinese Spring' (CS) revealed that wheat chromosomes 1D, 2B and 3D were involved in the three wheat-Thinopyrum chromosome translocations designated as (W-T)1, (W-T)2, and (W-T)3 respectively. The analysis of high-molecular-weight glutenin subunits in single seeds of AT 3425 confirmed the involvement of wheat chromosome 1D in the translocation (W-T)1. The designations 1DSx1DL-1TL, 2BSx2BL-2TL and 3DSx3DL-3TL were suggested for the wheat-Thinopyrum translocated chromosomes (W-T)1, (W-T)2 and (W-T)3 in AT 3425 respectively.

Chromosome Banding↗

PubMed2000

[Analysis of genetic diversity of wheat using genetic resources information system GRIS].

The possibilities of using the Genetic Resources Information and Analysis System (GRIS) 3.2, a database and software package, in studies of various aspects of genetic diversity in wheat are demonstrated. A model of genetic diversity was analyzed; diversity levels were estimated in wheat from various regions at different times. The genetic basis of Russian winter and spring common wheat cultivars was investigated. Transmission of powdery mildew resistance genes was traced in cultivars with identified Pm genes. For some genes, patterns of geographical distribution were characterized. Description of GRIS 3.2 is given: the package consists of a database on the global gene pool of wheat and software on genealogical and statistic analyses of genetic diversity.

Databases, Factual↗

PubMedDate not supplied

[Formation of phenol compounds in various cultivars of wheat (Triticum aestivum L.)].

The formation of soluble phenol compounds, including flavonols, was studied in winter (Erythrospermum, Lutescens 230, and R 47-28) and spring cultivars (Lada) of wheat (Triticum aestivum L.). The contents of soluble phenol compounds and flavonols were 1.8-2.6 and 0.5-1.3 mg/kg fresh weight, respectively. These results illustrate the similarity of phenol metabolism in leaves of winter and spring wheat cultivars. The exception was the cultivar R 47-28 that accumulated the maximum amount of phenol compounds (e.g., flavonols). In this cultivar the ratio of flavonols reached 50% of total soluble phenol content. In other cultivars, this parameter did not exceed 25-35%. The data indicate that the cultivar R 47-28 differs from other wheat cultivars in the metabolism of phenol compounds. The observed differences are probably related to genetic modifications of the cultivar R 47-28 during selection.

Phenols↗