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Bacterial Pyomyositis.

Bacterial pyomyositis has been defined as a subacute, deep bacterial infection of the soft tissues. The entity was originally described only in tropical climates, but it is increasing in incidence in temperate climates, such as in the United States. This is mainly attributed to the presence of immunocompromising states such as HIV/AIDS or liver disease. The etiology of pyomyositis remains a mystery, but its characteristics are well-described. If untreated, the disease process progresses through three distinct stages. The first stage is defined by vague complaints, muscle pain, and a low-grade fever. The second stage presents as worsening pain, swelling, fever, and actual abscess formation in the muscle. If the patient remains untreated, pyomyositis progresses to the third stage, which includes septicemia and possible septic shock. Large muscles around the pelvis are most commonly affected, and Staphylococcus aureus is the bacteria seen in most documented cases. To make a timely diagnosis and prevent serious sequelae, physical examination, laboratory results, imaging studies, and a high clinical suspicion must all be combined. Magnetic resonance imaging is the diagnostic study of choice, but it may be inconclusive early in the disease process. Definitive diagnosis must be made with aspiration or surgical drainage. Once diagnosed, treatment consists of appropriate intravenous antibiotics and possible surgical intervention. A full recovery is expected if appropriate treatment is initiated early in the process.

Journal Article↗

Chronic strongyloidiasis in Vietnam veterans.

Chronic Strongyloides stercoralis infections can produce intermittent urticarial skin eruptions and gastrointestinal discomfort for years. Three cases of symptomatic infection acquired by United States military personnel in Vietnam are presented as examples of chronic parasitic disease unrecognized by physicians despite clear histories of geographic exposure, classic creeping skin eruptions, and eosinophilia. More attention needs to be paid to the possible long-term sequela from military service in tropical climates by both military and civilian physicians.

Adult↗

Juvenile hormone titer in capped worker brood of Apis mellifera and reproduction in the bee mite Varroa jacobsoni.

Juvenile hormone (JH) titers were recorded from fifth instar worker larvae of Apis mellifera carnica, Apis mellifera lamarckii, and Africanized honeybees kept under temperate and tropical climatic conditions. No differences in hormone titer according to honeybee race or climatic conditions were determined. However, the rate of reproduction of the ectoparasitic mite, Varroa jacobsoni, on larvae of the different honeybee races was highly variable. The possible role of honeybee JH in control of the parasite's reproduction is discussed.

Animals↗

On the epidemiology of yaws in African miners (1942).

Several points of interest arise from these unusual human yaws infections: (i) European miners contracted a treponemal infection from Africans with yaws and developed yaws; (ii) the infectious patients who started the minor epidemics in the outbreaks must have been relapses from prolonged latent early infections; (iii) the time taken for the treponemes, possibly only a few minutes, to penetrate the skin through a minor injury seemed to be less than six hours; (iv) transmission occurred only at the working rock face deep underground with a tropical climate, and not on the surface 1,630 metres above sea-level, where there were abundant opportunities; (v) the search for treponemes in the environment of the working place was restricted by the inability to culture the treponeme.

Adolescent↗

The occurrence of the bedbugs Cimex hemipterus and Cimex lectularius in northern Natal and KwaZulu, South Africa.

Two species of anthropophilic bedbugs, Cimex lectularius and C. hemipterus, infest dwellings in KwaZulu, the known range of the latter extending to 28 degrees 15'S. C. hemipterus is the more common species in KwaZulu north of 27 degrees 15'. The pattern of distribution of C. hemipterus in South Africa is consistent with the species requiring a tropical climate, being a more recent invader than C. lectularius, and being able to thrive sympatrically with the latter species.

Animals↗

Experimental assessment of a new, low-cost antivenom for treatment of carpet viper (Echis ocellatus) envenoming.

Morbidity and mortality due to envenoming by the carpet viper (Echis ocellatus) in northern Nigeria remains unacceptably high and constitutes a severe economic and public health problem to the local farming community in particular. The only effective treatment of systemic envenoming is antivenom, but supplies are very limited as the little that is available is either too expensive, ineffective or both. Here, we describe a new ovine antivenom, designed both to be effective and to be available at low cost. The antivenom, a polyclonal ovine Fab preparation, provides superior protection, both in vivo and in vitro, to the best alternatives, the monospecific South African Institute of Medical Research antivenom and the polyspecific Pasteur Isper Africa antivenom. Fab fragments, which have the advantages of large volumes of distribution and, theoretically, low immuno-reactivity, are produced by a reusable solid-phase papain matrix which eliminates enzyme contamination of the product and reduces cost. The antivenom is lyophilised for increased stability and extended shelf-life in tropical climates where it is often impossible to keep such products cool.

Animals↗

Strategies for the control of one-host ticks and relationship with tick-borne diseases in South America.

A variety of hemoparasites occurs in ruminants of South America (12 degrees 00'N-56 degrees 00'S), but there is consensus on the significant economic impact of babesiosis (Babesia bovis and Babesia bigemina) and anaplasmosis (Anaplasma marginale). The tick vector for the Babesia spp. for the whole region is Boophilus microplus which has been the most important target in control/eradication programs. Boophilus microplus and many different hematophagous diptera species are considered vectors of A. marginale. In areas where babesiosis and anaplasmosis are present, the wide range of climates (tropical, subtropical, temperate), husbandry practices (subsistence, extensive, intensive), and tick control strategies has led to the concepts of enzootic stability and instability. Within this framework, six control strategies for Boophilus microplus can be identified: (1) absolute tick control--with the establishment of quarantine areas and the implementation of state legislation; (2) absolute tick control in marginal areas--using local epidemiological knowledge, strategic treatments, quarantine areas and the intervention of the state; (3) prophylactic treatment--with a predetermined acaricide treatment schedule aimed at reducing tick burdens to an acceptably low level; (4) threshold treatment--acaricides are applied by the farmer whenever the number of ticks on cattle exceed a threshold level; (5) opportunistic treatment--acaricides are applied when cattle are assembled for other purposes; (6) integrated tick control--principally involving the combination of acaricides with the use of tick-resistant crossbred cattle.

Anaplasmosis↗

Determination of chloroquine and its decomposition products in various brands of different dosage forms by liquid chromatography.

Various commercial preparations of chloroquine dosage forms have been examined both by thin-layer chromatography (TLC) and liquid chromatography (LC). TLC showed that all these preparations yielded more than one spot, indicating possible degradation. An LC method has been adapted for the determination of chloroquine in these drug formulations. The standard calibration curve was linear over the concentration range 1-6 micrograms ml-1. Chloroquine was assayed in various brands of different forms (ampoules, tablets and syrups). However, it was observed, for some samples, that the bands obtained were rather broad, showing shoulders or peak splitting, indicating the presence of other compounds coeluting with chloroquine. The utility of this method for the quality control of this major drug is assessed in the context of the need to carefully monitor drug purity in a tropical climate, particularly in situations where there may be doubt about the quality of the primary manufacturer.

Chloroquine↗

Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays.

Aspergillus species included in section Nigri are common in plant products and processed food, such as grapes, cereals, coffee and derivatives, particularly in warm and tropical climates. Two of these species, A. carbonarius and A. niger, are known to produce ochratoxin A (OTA), a potent nephrotoxin and carcinogenic to human (group 2B). Recognition of the several species of this section is difficult and requires considerable expertise using conventional methods based on morphological features. In this work we describe rapid, sensitive and robust assays based on the PCR technique to discriminate the main species included in section Nigri: A. japonicus, A. heteromorphus, A. ellipticus and the two morphologically indistinguishable species of the A. niger aggregate: A. niger and A. tubingensis. The species-specific primers have been designed on the basis of ITS (internal transcribed spacers of rDNA units) sequence comparisons obtained from several Aspergillus strains and have been tested in a number of strains from different origins and hosts. These PCR assays, based on multi-copy sequences, are highly sensitive and specific and represent a good tool for an early detection of OTA-producing Aspergillus species in order to prevent OTA from entering the food chain.

Aspergillus↗

The impact of HIV infection on tropical diseases.

HIV and tropical infections affect each other mutually. HIV infection may alter the natural history of tropical infectious diseases, impede rapid diagnosis, or reduce the efficacy of antiparasitic treatment. Tropical infections may facilitate the transmission of HIV and accelerate progression from asymptomatic HIV infection to AIDS. This article reviews data on known interactions for malaria, leishmaniasis, human African trypanosomiasis, Chagas' disease, schistosomiasis, onchocerciasis, lymphatic filariasis, and intestinal helminthiases.

AIDS-Related Opportunistic Infections↗

Post-LASIK myopic shift after a trek in the North Pole.

Bilateral laser in situ keratomileusis was performed in a 37-year-old male ethnic Chinese adventurer. Eight weeks postoperatively, the patient made an unassisted trek to the geographic North Pole. After 2 weeks in this environment, a myopic shift of more than -1.50 diopters occurred in both eyes. The myopic shift resolved over 2 months when the patient returned to a tropical climate. Using Orbscan corneal topography, we propose that the myopic shift was attributed to biomechanical changes in the cornea induced by corneal dehydration in the Arctic environment.

Adult↗

Chlorinated pesticides and PCBs in the sea-surface microlayer and seawater samples of Singapore.

Sea-surface microlayer (SML) and seawater samples collected from Singapore's coastal marine environment were analyzed for selected chlorinated pesticides and polychlorinated biphenyls (PCBs). The SML is a potential site of enrichment of persistent organic pollutants (POPs) compared to the underlying water column. The concentration ranges of SigmaHCH, SigmaDDT and SigmaPCB in subsurface (1 m depth) seawater were 0.4-27.2 ng/l (mean 4.0 ng/l), 0.01-0.6 ng/l (mean 0.1 ng/l) and 0.05-1.8 ng/l (mean 0.5 ng/l) respectively. In the SML, the concentration ranges of SigmaHCH, SigmaDDT and SigmaPCB were 0.6-64.6 ng/l (mean 9.9 ng/l), 0.01-0.7 ng/l (mean 0.2 ng/l) and 0.07-12.4 ng/l (mean 1.3 ng/l) respectively. High spatial and temporal distribution was observed for all POPs measured. However, overall levels measured in the SML were lower than levels reported in the literature for SML samples from temperate coastal regions-possibly due to loss of semi-volatile compounds in the tropical climate of Singapore. Atmospheric wet deposition during the monsoon season may be an important source of POPs to the SML. This study provides the first scientific data on POP concentrations and enrichment factors in the SML for Southeast Asia.

Environmental Monitoring↗

Pesticide contamination of the coastline of Martinique.

In January and February 2002, the presence of certain agricultural pesticides throughout the coastline of the Caribbean island of Martinique was investigated. The tropical climate of the French West Indies is suitable for banana production, which requires intensive use of pesticides. An inventory of all pesticides used on the island (compounds and tonnage) was compiled. Surveys and analyses revealed the presence of pesticides in the plumes of seven rivers. The organochlorine chlordecone and metabolites of aldicarb were detected at nearly all of the monitored sites, even though the use of chlordecone has been prohibited since 1993. Two triazines (ametryn and simazine) were also identified. The concentrations of carbamates and triazines detected in the water and sediment samples from Martinique are comparable to those reported for mainland France. Chlordecone concentrations in the sediment and particulate matter samples were, however, particularly high in the samples from Martinique. Toxicological implications are discussed. Of particular concern are the high levels of chlordecone (which is bioaccumulating and carcinogenic) and further monitoring of this compound is recommended, especially in fish and other sea-food products.

Aldicarb↗

[Chikungunya virus infection: review through an epidemic].

The Chikungunya virus is an alpha arbovirus, first identified in 1953, transmitted by Aedes, mosquitoes, responsible for a little documented uncommon acute specifically tropical disease. Its main symptoms are fever, a rash, and debilitating arthralgia. An unprecedented Chikungunya epidemic is ongoing on the Reunion Island (775,000 inhabitants) with over 244,000 reported and 205 deaths (directly or indirectly linked) as of April 20 2006. Aedes albopictus, long present on the island, is the assumed vector. It had already been identified as the vector for type 2 Dengue fever in 1997-1978 (200,000 cases) for type 1 Dengue fever in 2004 (300 cases). After the Grande Comore Island epidemic, the first cases were reported in the Reunion Island in March 2005. The epidemic was a surprise because of its unexpected emergence, its magnitude, and clinical cases rarely or never described before: severe forms, central neurological involvement, hepatic cytolyse, severe lymphopenia, severe dermatological involvement, deaths, and neonatal infections. This is the first manifestation of the intrusion CHK virus on the island, which benefits from a sub-tropical climate, but also of an occidental healthcare environment, with a non-immune population. This is also the first time that a Chikungunya epidemic is described in this part of the world.

Aedes↗

Natural hypometabolism during hibernation and daily torpor in mammals.

Daily torpor and hibernation are the most powerful measures of endotherms to reduce their energy expenditure. During entrance into these torpid states metabolic rate is suppressed to a fraction of euthermic metabolism, paralleled by reductions in ventilation and heart rate. Body temperature gradually decreases towards the level of ambient temperature. In deep torpor body temperature as well as metabolic rate are controlled at a hypothermic and hypometabolic level. Torpid states are terminated by an arousal where metabolic rate spontaneously returns to normal levels again and euthermic body temperature is established by a burst of heat production. In recent years some of the cellular mechanisms which contribute to hypometabolism have been disclosed. Transcription, translation, as well as protein synthesis are largely suppressed. Cell proliferation in highly proliferating epithelia like the intestine is suspended. ATP production from glucose is reduced and lipids serve as the major substrate for remaining energy requirements. All these changes are rapidly reverted to normometabolism during arousal. Hibernation and daily torpor are found in small mammals inhabiting temperate as well as tropical climates. It indicates that this behaviour is not primarily aimed for cold defense, instead points to a general role of hypometabolism, as a measure to cope with a timely limited or seasonal bottleneck of energy supply.

Adenosine Triphosphate↗