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The effect of spices on cholesterol 7 alpha-hydroxylase activity and on serum and hepatic cholesterol levels in the rat.

The effect of feeding curcumin, capsaicin, ginger, mustard, black pepper and cumin on cholesterol and bile acid metabolism was studied in rats. The activity of hepatic cholesterol-7 alpha-hydroxylase, the rate-limiting enzyme of bile acid biosynthesis, was significantly elevated in curcumin (turmeric), capsaicin (red pepper), ginger and mustard treated animals. The enzyme activity was comparable to controls in black pepper and cumin fed rats. Serum and liver microsomal cholesterol contents were significantly higher in the curcumin and capsaicin treated animals. Thus, this study has suggested that the spices--turmeric, red pepper, ginger and mustard can stimulate the conversion of cholesterol to bile acids, an important pathway of elimination of cholesterol from the body. However, simultaneous stimulation of cholesterol synthesis by the spice principles--curcumin and capsaicin suggests that there may not be any significant contribution of stimulation of bile acid biosynthesis to the hypocholesterolemic action of these spices, and the latter action may solely be due to interference with exogenous cholesterol absorption.

Animals↗

Comparison of three methods for determining aflatoxins in sunflower seed meals.

Methods developed for the determination of aflatoxins in peanut products, in cottonseed products, and in ginger were compared for their applicability to sunflower seed meal. The peanut products method was inapplicable. Separation from interferences adequate to obtain a limit of determination for aflatoxin B1 around 1 ng/g and recovery of total added aflatoxins of about 97% could be achieved with either the cottonseed or ginger methods. The cleanest extract and the most rapid operation were obtained with the ginger method.

Aflatoxins↗

Smoke as a form of personal protection against mosquitos, a field study in Papua New Guinea.

Smoke from burning different kinds of vegetation or wood is often used as a cheap personal protection measure against mosquitos during the evening. To test the efficacy of smoke, a comparative field trial was conducted in the Wosera District, Papua New Guinea. Repellency of smoke from burning wild mango wood (Mangifera spp), leaves from betelnut (Areca catechu), wild ginger (Alpinia spp) and coconut husks (Cocos nutifera) was assessed using man-biting catches. A mosquito species and smoke specific repellency was found. An. karwari was repelled by coconut husks (66% CI 17-86%), ginger (69% CI 25-87%) and betelnut (84% CI 62-94%) leaves. Culicines were repelled by mango wood (57% CI 6-80%), coconut husks (62% CI 18-83%), ginger (75% CI 45-88%) and betelnut (64% CI 22-84%) leaves. For An. koliensis no repellency due to smoke was found. In combination with untreated or impregnated bed-nets, smoke may contribute to a reduction of mosquito transmitted diseases.

Animals↗

Protective activities of some phenolic 1,3-diketones against lipid peroxidation: possible involvement of the 1,3-diketone moiety.

The protective activities of four ginger-derived phenolic 1,3-diketones (1-4) and curcumin (5) against lipid peroxidation was studied by using different biologically relevant model systems and pulse radiolysis. The extraordinary activity of 5 vis-à-vis 1-4 against Fe(2+)-mediated peroxidation may be attributed to the additional phenolic hydroxy group in the former, which lends it better iron-chelating and radical-scavenging properties. In iron-independent peroxidation, however, the ginger constituent [6]-dehydrogingerdione (1) showed activity comparable to that of 5; this indicates its higher affinity for the lipid peroxide radical (LOO(.)), due to its higher hydrophobicity. A very high rate constant for the reaction between 1 and Cl(3)COO(.), measured by pulse radiolysis, not only confirmed this, but also established the superior antioxidant efficacy of 1 in comparison to vitamins E and C. This was also evident from the results obtained from a liposomal peroxidation study with 1 and vitamin C. This study also established a synergistic effect of the latter on the antioxidant activity of 1. HPLC analysis of the products of the reaction between 1 and Cl(3)COO(.) revealed the formation of higher concentrations of ferulic acid (7), along with vanillin (6). The presence of ascorbate affected the generation of 7 more than it did that of 6. On this basis, a mechanism for the antioxidant action of 1 has been proposed, which suggests the contribution of the phenolic group as well as the active methylene group of the 1,3-diketones.

Animals↗

Neuroprotective effect of N-acetylcysteine on neuronal apoptosis induced by a synthetic gingerdione compound: involvement of ERK and p38 phosphorylation.

Besides being used as a spice, ginger has been applied in oriental medicine to ameliorate symptoms such as inflammatory, rheumatic disorders, and gastrointestinal discomforts. The effects of ginger on neuronal cells, however, have not been explored. We investigate the effect of 1-(3,4-dimethoxyphenyl)-3,5-dodecenedione (I(6)), a derivative of gingerdione, on cultured cortical neurons. After a 5-day maturation period in vitro, cortical neurons were treated with I(6) for 24 hr and cell viability was assessed using MTT assay. I(6) induced neuronal death in a concentration-dependent manner. Hoechst 33342, propidium iodide (PI), and TUNEL staining confirmed that the reduced cell viability by I(6) was due to apoptosis. Pre-treatment of cell with N-acetylcysteine (NAC) prevented cell death in a concentration-dependent manner. N-acetylcysteine increased phosphorylated levels of p42 and p44 extracellular signal-regulated kinases (ERKs). In parallel, farnesyltransferase and MEK inhibitors blocked ERK phosphorylation and neuroprotective effect of NAC. Unexpectedly, NAC also increased phosphorylated level of p38 mitogen-activated protein kinase (MAPK) and p38 specific inhibitors dose-dependently attenuated the effect of NAC. Farnesyltransferase and MEK inhibitors completely abolished NAC-induced p38 phosphorylation whereas p38 inhibitor did not influence NAC-induced ERK phosphorylation. These results show that NAC serially activates ERKs and p38 MAPK, and ERKs and p38 work together to mediate the neuroprotective effect of NAC.

Acetylcysteine↗

In vitro susceptibility of Helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders.

The gram-negative bacterium Helicobacter pylori (HP), identified in 1982, is now recognized as the primary etiological factor associated with the development of gastritis and peptic ulcer disease. In addition, HP infections are also associated with chronic gastritis, gastric carcinoma and primary gastric B-cell lymphoma. For centuries, herbals have been used in traditional medicine to treat a wide range of ailments, including gastrointestinal (GI) disorders such as dyspepsia, gastritis and peptic ulcer disease (PUD). However, the mechanism of action by which these botanicals exert their therapeutic effects has not been completely elucidated. As part of an ongoing screening program, the study assessed the in vitro susceptibility of 15 HP strains to botanical extracts, which have a history of traditional use in the treatment of GI disorders. Methanol extracts of Myristica fragrans (seed) had a MIC of 12.5 microg/mL; Zingiber officinale (ginger rhizome/root) and Rosmarinus officinalis (rosemary leaf) had an MIC of 25 microg/mL. Methanol extracts of botanicals with a MIC of 50 microg/mL included Achillea millefolium, Foeniculum vulgare (seed), Passiflora incarnata (herb), Origanum majorana (herb) and a (1:1) combination of Curcuma longa (root) and ginger rhizome. Botanical extracts with a MIC of 100 microg/mL included Carum carvi (seed), Elettaria cardamomum (seed), Gentiana lutea (roots), Juniper communis (berry), Lavandula angustifolia (flowers), Melissa officinalis (leaves), Mentha piperita (leaves) and Pimpinella anisum (seed). Methanol extracts of Matricaria recutita (flowers) and Ginkgo biloba (leaves) had a MIC > 100 microg/mL.

Curcuma↗

Instrument dependence of electrospray ionization and tandem mass spectrometric fragmentation of the gingerols.

The gingerols, including [6]-, [8]-, and [10]-gingerols, a series of chemical homologs differentiated by the length of their unbranched alkyl chains, have been identified as major active components in fresh ginger rhizome. The purpose of this study was to investigate the utility of ion trap liquid chromatography/tandem mass spectrometry (LC/MS/MS) as an online tool to identify and quantify these compounds in raw or processed ginger rhizome samples. Negative mode electrospray ionization (ESI) was used in MS, MS/MS and MS(n) experiments in quadrupole ion trap instruments from two different manufacturers and in high-resolution and accurate mass MS and MS/MS experiments in a Fourier transform ion cyclotron resonance mass spectrometer to elucidate the ionization and fragmentation mechanisms of these compounds in these instruments. Positive mode ESI, which generated many more fragment ions in full scan MS even under gentle ionization conditions, was also used in LC/MS and MS/MS experiments and in direct infusion MS and MS/MS experiments. Consistent and predictable ionization and fragmentation behaviors were observed for all gingerols when analyzed in the same instrument. Instruments from different manufacturers, however, had different ionization mechanisms. The major difference between instruments was their ability to form covalent dimer adducts of the gingerols. Subsequent fragmentation patterns of the precursor ions were essentially identical. These results clearly demonstrate that LC/MS instruments produce data that cannot necessarily be replicated in other laboratories, especially if those laboratories do not have the same instrument model from the same manufacturer. This presents major problems for metabolite target analysis, metabolic profiling and metabolomics investigations, which would benefit from LC/MS mass spectrum libraries as they do from GC/MS mass spectrum libraries, because such libraries may not be valid across platforms.

Catechols↗

Effects of gamma irradiation on the flavor composition of food commodities.

Fifteen food products including potato, sweet potato, shallot, onion, garlic, ginger, papaya, mango, rice, tobacco, small red bean, mungbean, soybean, wheat, flour and spices have been approved for irradiation by the National Health Administration in Taiwan. Market tests (Wu et al., 1996) provided strong proof that Taiwanese consumers would accept irradiated foods. However, researchers in the food industry are concerned about the possibility of chemical changes, especially in volatile composition, during irradiation processing. This study considers several food commodities, including garlic, ginger, shiitake, onion, potato, day-lily, tilapia, silver carp and shrimp. Food samples were irradiated with optimum doses and then studied for possible occurrence of chemical changes and effects on compositional characteristics of foods.

Animals↗

Lethal efficacy of extract from Zingiber officinale (traditional Chinese medicine) or [6]-shogaol and [6]-gingerol in Anisakis larvae in vitro.

The authors previously reported that an extract from Zingiber officinale, traditionally eaten along with raw fish and used in traditional Chinese medicine, effectively destroyed Anisakis larvae in vitro. In this study, we analyzed the effective components of ginger rhizomes. Methanol extracts were fractionated after first being treated with HCl at pH 3, then with NaHCO3 at pH 10, and, finally, with NaOH at pH 13 (fraction 1). In general, this fraction is rich in neutral substances. [6]-Shogaol and [6]-gingerol, known neutral components of ginger rhizomes, were detected using gas chromatography and were found to be the most prevalent components in the fraction, occurring in quantities that resulted in a dose-dependent killing efficacy. Authentic [6]-shogaol and [6]-gingerol could kill Anisakis larvae at a minimal effective dose of 62.5 and 250 micrograms/ml, respectively. However, the concentration of [6]-gingerol in fraction 1 was greater than 20 times that of [6]-shogaol, making the former the most active component in the fraction. Furthermore, synergistic effects between [6]-gingerol and a small amount of [6]-shogaol were observed. Pyrantel pamoate, an available antinematodal drug, had no lethal effect, even at a concentration of 1 mg/ml. In saline solution containing [6]-shogaol (62.5 micrograms/ml), greater than 90% of larvae lost spontaneous movement within 4 h and were destroyed completely within 16 h. Microscopical examinations showed destruction of the digestive tract and disturbances of culticulae.

Animals↗

Common Indian spices: nutrient composition, consumption and contribution to dietary value.

Nutrient composition of eight commonly consumed spices of South India was analysed. Spices analysed were red chillies (Capsicum annum), black pepper (Piper nigrum), coriander seeds (Coriandrum sativum), cumin seeds (Cuminum cyminum), garlic (Allium sativum), asafoetida (Ferula foetida), dry ginger (Zingiber officinale) and ajowan (Carum copticum). The nutrients analysed were proximate principles, minerals, starch, sugars, dietary fibre components, tannins, phytic acid, enzyme inhibitors and amino acids. Dry ginger, ajowan and asafoetida had high calcium (1.0-1.5%) and iron (54-62 mg/100 g) levels. The tannin content of spices was also high (0.9-1.3% DM). Dietary fibre ranged from 14-53%. Spices had appreciable amounts of essential amino acids like lysine and threonine. A survey revealed the average per capita consumption of spices to be 9.54 g and at that level, the nutrient contribution from spices ranged from 1.2 to 7.9% of an average adult Indian male's requirement for different nutrients.

Amino Acids↗

Effect of diet consistency, taste and calories on food intake of weanling rats with dorsomedial hypothalamic lesions.

Male weanling Sprague-Dawley rats with dorsomedial hypothalamic lesions (DMNL rats) primarily destroying the dorosomedial hypothalamic nuclei (DMN) showed significant hypophagia on lab chow chunks compared to sham-operated controls. When given a choice between lab chow in the form of chunks or powder, both controls and DMNL rats ate similar amounts of lab chow powder while DMNL rats ate less lab chow chunks. Total caloric consumption was the same as on chunks alone. When returned to lab chow chunks as the only source of calories, the pattern and magnitude of intake was again depressed for the DMNL rats. When offered a choice between Ginger Snaps cookies in chunk form versus powder, DMNL rats remained hypophagic in terms of chunk consumption while the intake from powder was similar in both controls and DMNL rats. When offered a choice between chunks of lab chow and Ginger Snaps, DMNL rats were again hypophagic on lab chow chunks, ate the same as the controls of the cookies, and the total caloric intake was of the same magnitude and pattern as observed in previous tests. The data suggest, but do not conclusively show, that DMNL rats are not hypophagic because they have an aversion to chewing hard food and that, when offered a diet similar in hardness to lab chow chunks i.e., hard cookies, will prefer the less tasty but nutritionally complete lab chow. They are apparently capable of choosing a diet for complete nutrition and, as previously reported, can meter calories competently.

Animal Nutritional Physiological Phenomena↗

Immunoglobulin E antibodies against coriander and other spices.

Patient M, working with spices in the food industry, developed asthma on inhalation of dust from spices. Skin prick test results with curry, coriander, and mace were strongly positive. With RAST, specific IgE antibodies against coriander, curry, mace, ginger, and paprika powder could be demonstrated in serum from patient M. It could be demonstrated by RAST inhibition that the IgE-binding components from coriander did not cross-react with the IgE-binding components from ginger and paprika. Leukocytes from a normal donor, after passive sensitization with serum from patient M, released a substantial (greater than or equal to 50%) amount of histamine on challenge with extracts of coriander, mace, and curry powder. This study demonstrates that the inhalation of dust from spices can result in an IgE-mediated allergy toward these materials.

Adult↗

The active part of the [6]-gingerol molecule in mutagenesis.

A study was performed to discover the active part in mutagenesis of [6]-gingerol, a mutagen contained in ginger Zingiber officinale (Nakamura and Yamamoto, 1982). [6]-Shogaol was isolated from the ginger by column chromatography on silica gel. [6]-Shogaol was much less mutagenic (1 X 10(3) revertants/10(8) viable cells/700 microM) than [6]-gingerol (1 X 10(7) of the same units). Mutation frequencies of their related compounds were 4 X 10(1) for zingerone, 1 X 10(7) for 3-hydroxymyristic acid and 3 X 10(2) for 12-hydroxystearic acid. Curcumin, and myristic, stearic and oleic acids had no mutagenicity; and diacetone alcohol and butyroin were suppressible for mutation. It was inferred from these results that the active part of [6]-gingerol was the aliphatic chain moiety containing a hydroxy group.

Catechols↗

Mutagenicity of gingerol and shogaol and antimutagenicity of zingerone in Salmonella/microsome assay.

Ginger extract and its constituents gingerol, shogaol and zingerone were tested in Salmonella typhimurium strains TA 100, TA 98, TA 1535 and TA 1538 in the presence and in absence of S9 mix. It was observed that ginger extract, gingerol and shogaol were mutagenic on metabolic activation in strains TA 100 and TA 1535, but zingerone was non-mutagenic in all the four strains with or without S9 mix. When mutagenicity of gingerol and shogaol was tested in presence of different concentrations of zingerone it was observed that zingerone suppressed mutagenic activity in both the compounds in a dose dependent manner.

Animals↗

An infrared radiation study of the biophysical characteristics of traditional moxibustion.

OBJECTIVES: Moxibustion has been a part of acupuncture practice for thousands of years. Traditionally, it includes direct moxibustion, in which moxa sticks are burned at acupuncture points on the skin, and indirect moxibustion, in which monkshood cakes or ginger or garlic slices are used to insulate the skin from burning moxa cones. Recently randomised clinical trials and clinical observations suggest that moxibustion can enhance physiological and immune functions, but there has been little investigation of the scientific basis of these traditional techniques. The present study compared the infrared radiation caused by these techniques to that of non-specific controls and to that of the human body surface at an acupuncture point. METHODS: A highly sensitive, infrared-spectrum detection device was used to compare the spectra of traditional moxibustion materials (n = 4/group) with those of control materials (n = 4/group) and to the spectrum at the surface of an acupuncture point LI 4 (Hegu) in healthy volunteers (n = 7). RESULTS: The infrared radiation intensity produced by a traditional moxa stick was 43300.41 mV, with a peak on the infrared spectrum of 3.5 microm, while the respective radiation intensities of two controls, a smokeless moxa stick and a 555 cigarette, were 31.15 mV and 37.03 mV with peaks of 7 microm and 3.5 microm. The infrared radiation intensities of the three traditional media of indirect moxibustion, monkshood cake, ginger slices and garlic slices, were 520.27 mV, 594.79 mV and 681.87 mV, respectively, all with peaks around 7.5 microm and similar spectra. In contrast, the infrared radiation intensities of slices of cucumber and carrot, used as control media for indirect moxibustion, were 274.47 mV and 50.53 mV, respectively, substantially different from those of the traditional media. Infrared radiation at LI 4 (Hegu) was 20.40 mV, and peaked on the infrared spectrum at about 7.5 microm. The experiment showed that the thermal action of the traditional moxa stick was more potent than that of indirect moxibustion and its radiation peak was different from that at the acupuncture point on the human body. In contrast, the thermal action of traditional indirect moxibustion was modest and its radiation peak matched that at the acupuncture point. CONCLUSION: Direct moxibustion with a traditional moxa stick may produce its potent therapeutic effects by thermal action, while traditional indirect moxibustion may act by producing modest thermal action and a sympathetic vibration at the skin surface. Non-traditional thermal materials and media may not be suitable substitutes for traditional materials. The data provide a scientific, biophysical rationale for traditional moxibustion.

Adult↗

The herbal medicine Dai-kenchu-to and one of its active components [6]-shogaol increase intestinal blood flow in rats.

The present study investigated the effects of the herbal medicine Dai-kenchu-to (DKCT) and its 4 individual ingredients on intestinal blood flow (IBF) in rats by laser Doppler flowmetry. Intraduodenal administration of DKCT (30, 100 and 300 mg/kg) increased IBF in a dose-dependent manner, whereas the mean arterial blood pressure was not affected. One of the ingredients in DKCT is dried ginger rhizome (150 mg/kg), whose main component is [6]-shogaol (2 mg/kg), both of which showed similar effects to those shown by DKCT, while the other ingredients in DKCT only slightly increased IBF or had no effect. The calcitonin gene-related peptide (CGRP) receptor antagonist, CGRP (8-37), completely abolished the hyperemia induced by DKCT, dried ginger rhizome and [6]-shogaol. However, the vasoactive intestinal polypeptide (VIP) receptor antagonist, [4-Cl-DPhe6, Leul7]-VIP, and atropine were less inhibitory than CGRP (8-37), and the substance P (SP) receptor antagonist, spantide, had no effect. The present study demonstrated that DKCT and one of its active components, [6]-shogaol, produced an increase in IBF which was mainly mediated by CGRP and suggests that DKCT may be useful in the treatment of intestinal ischemia-related diseases.

Animals↗

Alternative therapies for nausea and vomiting of pregnancy.

OBJECTIVE: To review available evidence about the effectiveness of alternative therapies for nausea and vomiting of pregnancy. DATA SOURCES: MEDLINE and 13 additional US and international data bases were searched in 1996-1997 for papers that described use of alternative medicine in the treatment of pregnancy and pregnancy complications, specifically those addressing nausea, vomiting, and hyperemesis. Bibliographies of retrieved papers were reviewed to identify additional sources. METHODS OF STUDY SELECTION: All relevant English language clinical research papers were reviewed. Randomized clinical trials addressing specifically the use of nonpharmaceutical and nondietary interventions were chosen for detailed review. TABULATION, INTEGRATION, AND RESULTS: Ten randomized trials studying the effects of acupressure, ginger, and pyridoxine on nausea and vomiting of pregnancy were reviewed. Evidence of beneficial effects was found for these three interventions, although the data on acupressure are equivocal. Insufficient evidence was found for the benefits of hypnosis. Other interventions have not been studied. CONCLUSION: There is a dearth of research to support or to refute the efficacy of a number of common remedies for nausea and vomiting of pregnancy. The best-studied alternative remedy is acupressure, which may afford relief to many women; ginger and vitamin B6 also may be beneficial.

Acupressure↗

Induction of apoptosis in HL-60 cells by pungent vanilloids, [6]-gingerol and [6]-paradol.

[6]-Gingerol, a major pungent ingredient found in the rhizome of ginger, has been reported to possess a strong antiinflammatory activity, which is considered to be closely associated with its cancer chemopreventive potential. [6]-Paradol, another pungent phenolic substance found in ginger and other Zingiberaceae plants, also has a vanilloid structure found in other chemopreventive phytochemicals including curcumin. In the present study, [6]-gingerol and [6]-paradol were found to exert inhibitory effects on the viability and DNA synthesis of human promyelocytic leukemia (HL-60) cells. The cytotoxic and antiproliferative effects of both compounds were associated with apoptotic cell death. The above results suggest that [6]-gingerol and [6]-paradol possess potential cytotoxic/cytostatic activities.

Anticarcinogenic Agents↗