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Listing of color additives for coloring sutures; [phthalocyaninato(2-)] copper. Food and Drug Administration, HHS. Final rule.

The Food and Drug Administration (FDA) is amending the color additive regulations to provide for the safe use of [phthalocyaninato(2-)] copper in coloring nonabsorbable sutures for general and ophthalmic surgery made from a blend of poly(vinylidene fluoride) and poly(vinylidene fluoride-co-hexafluoropropylene). This action responds to a petition filed by Ethicon, Inc.

Color↗

[Comparative in vitro evaluation of color and color stability of composite and ceramic ionomer materials for esthetic repair of teeth].

Comparative colorimetric evaluation of shades and translucency of different samples, fabricated from Filtec Z-250, Unirest, Dyract AP and Vitremer, shows, that differences in their compositions and industrial technologies result to great differences between the shades of the same number of Vita Shade Guide. The less color stability after long term action by foods media to the samples was found for the glass ionomer cement. The best color stability was found for composites, partially, for Unirest. All of these, let us conclude, that the main changes, not only in shades, but in translucency of materials, as a result the action of model media, take place at the early periods (the first month). Usually, the most main changes in restorative materials take place in this period of beginning and connect with the end of setting processes and achieving the balances between water absorption and solubility.

Color↗

[Patient-related color preference and color design in the hospital].

Colour-physiological studies involving volunteers and colour-psychological interviewing of patients with varied diagnoses revealed that, currently, no compelling scientific reasons can be given for coloration in the hospital. Interviewing 68 patients from a number of clinics on the coloration of sick-rooms yielded the following preferred colours, irrespective of the patients' classing into groups by various criteria: Light colours were consistently preferred for all objects such as ceiling, walls, floor, curtains, furniture, and bed linen. It should be emphasized that even white was a desirable colour for ceilings and bed linen. Beige was found to rank first for walls, floors, curtains, and furniture, whereas the colours of wood and grey shared the second rank for floors and furniture. Green and pink ranked second for bed linen. White and green ranked second for walls and curtains, respectively. In view of the heterogeneity, the findings may not be converted into an absolute just as the recommendations by other workers. Still, the patients' wish should be given priority over decreed recommendations by experts when basic considerations of illumination (reflectance!) are allowed for in the particular overall colour design project.

Color↗

[Coat color in dogs. 1: Basics of coat color genesis].

Within this brief review of literature the biochemical and physiological basics of coat colour genetics in the domestic dog are described. Parallels to the wildcolour of the wolf are shown, out of which the most of the today known colour-mutations developed. Furthermore the 10 most important of the today known coat color genloci are listed and some breed and nomenclature examples are also given. As the actual knowledge concerning the coat color inheritance is not able yet to explain some occurring coat colours and -combinations, an all-breed including nomenclature is not possible until today.

Animals↗

Homogeneity of large-field color matches in congenital red-green color deficients.

Rayleigh matches obtained from red-green color deficients with conventional methods show large individual differences within diagnostic categories. Similar matches obtained from the same observers with a large-field substitution method show much less variability and suggest that the differences observed among simple anomals, extreme anomals, and dichromats with conventional methods are probably not solely due to the visual pigments contained in the cones. A theory that attributes these differences to the relative number of abnormal cones present in the observer's retina is described.

Color Perception↗

Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and (TTAGGG)n by one-color and double-color FISH in the toadfish Halobatrachus didactylus (Teleostei: Batrachoididae).

The karyotype of Halobatrachus didactylus presents 46 chromosomes, composed of eight metacentric, 18 submetacentric, four subtelocentric, and 16 acrocentric chromosomes. The results of FISH showed that the major ribosomal genes were located in the terminal position of the short arm of a large submetacentric chromosome. They also showed a high variation in the hybridization signals. The products of amplification of 5S rDNA produced bands of about 420 pb. The PCR labeled products showed hybridization signals in the subcentromeric position of the long arm of a submetacentric chromosome of medium size. Double-color FISH indicated that the two ribosomal families are not co-located since they hybridized in different chromosomal pairs. Telomeres of all the chromosomes hybridized with the (TTAGGG)n probe. The GATA probe displayed a strong signal in the long arm of a submetacentric chromosome of medium size, in the subcentromeric position. The double-color FISH showed that the microsatellite GATA and the 5S rDNA gene are located in different chromosomal pairs. The majority presence of GATA probes in one pair of chromosomes is unusual and considering its distribution through different taxa it could be due to evolutionary mechanisms of heterochromatine accumulation, leading to the formation of differentiated sex chromosomes.

Animals↗

HER-2 testing in breast cancer using immunohistochemical analysis and fluorescence in situ hybridization: a single-institution experience of 2,279 cases and comparison of dual-color and single-color scoring.

We analyzed concordance between immunohistochemical analysis and fluorescence in situ hybridization (FISH) in HER-2 status and studied the effect of dual-color (D-FISH) vs single-color FISH (S-FISH) scoring on the assignment of tumors to amplified or nonamplified categories. The assays were performed on formalin-fixed, paraffin-embedded sections of 2,279 invasive breast carcinomas. Immunohistochemical results were interpreted as negative (0, 1+) or positive (2+, 3+). For FISH analyses, a ratio for HER-2/chromosome 17 of 2.0 or more (D-FISH) or an absolute HER-2 copy number per nucleus of more than 4.0 (S-FISH) were interpreted as positive gene amplification. We found 547 (24.0%) cases positive immunohistochemically, 326 (14.3%) by D-FISH, and 351 (15.4%) by S-FISH. Overall concordance in HER-2 status with immunohistochemical analysis was 87% for D-FISH and 86% for S-FISH. Excellent concordance was found among groups scored immunohistochemically as 0, 1+, and 3+ (with D-FISH, 97%; with S-FISH, 96%). The most discordant category was the group scored 2+ immunohistochemically, in which only a quarter of the 2+ tumors were FISH(+). D-FISH and S-FISH scoring results were discordant in 89 tumors (4%), of which 8 (9%) had 3+ immunohistochemical staining and none showed high-level HER-2 amplification. Among all FISH(+) tumors, 10% were negative by immunohistochemical analysis, and notably almost half (47%) showed borderline to low HER-2 amplification (D-FISH score, 2.0-3.9); the clinical significance of these findings warrants further investigation.

Adenocarcinoma↗

Color-contrast staining of two different lymphocyte subpopulations: a two-color modification of alkaline phosphatase monoclonal anti-alkaline phosphatase complex technique.

A dual staining method for different human lymphocyte subpopulations with nonoverlapping antigen distribution patterns is described. Cytocentrifuge slide preparations of peripheral blood nonadherant mononuclear cells (NAMNC), bone marrow aspirate or buffy coat smears were fixed in acetone and incubated with a primary mouse monoclonal antibody (MAb) against a lymphocyte antigen (CD8, Ig-light-chain, CD19, CD4) followed by rabbit anti-mouse immunoglobulin (Ig) and the alkaline phosphatase monoclonal anti-alkaline phosphatase (APAAP) complex. After repeating the "bridge" antibody and the APAAP, a red product was developed with fast red TR-naphthol AS-BI phosphate. Following this one-color stain the process was repeated using a different primary mouse MAb against another lymphocyte antigen (CD4, Ig-light chain, CD3, MHCII DR, CD5) and fast blue BB-naphthol AS-MX phosphate at the last step to yield a blue product. Control slides stained by the standard one-color APAAP method with the relevant primary MAb showed that there was no nonspecific labelling and the percent of positive cells in a given test was almost identical. To achieve an intense blue in the second stain for some antigens, e.g., CD4, either the MAb concentration had to be increased or two different MAbs recognizing differing epitopes of the same antigen, e.g., T1 and UCHT2 for CD5, were applied. Any change of red to purple at the site of the first stain after 15 min exposure to the blue-yielding AP substrate is due to residual AP activity of the first stain rather than to crossbinding of immunoreagents.(ABSTRACT TRUNCATED AT 250 WORDS)

Alkaline Phosphatase↗

Endothoracic sonography with color Doppler availability during video assisted thoracic surgery (videothoracoscopic operative staging with ultrasound color Doppler) for lung cancer staging.

BACKGROUND: The preoperative staging of lung cancer can be problematical when we attempt to evaluate T factor (T2-T3 versus T4) and N factor (N0 versus N1-N2). In some cases, radiology tests (CT scan, magnetic resonance imaging) cannot entirely dispel the possibility that the mediastinal structures have been infiltrated. N factor is evaluated mainly by dimensional criteria. However, mediastinoscopy and mediastinotomy do not allow the full exploration of all mediastinal mode stations. METHOD: Starting in 1995, we submitted 10 consecutive patients to videothoracoscopic operative staging with ultrasound color Doppler (VOS-USCD). In five cases, preoperative staging showed possible infiltration of the pulmonary artery (T4). In nine cases, we found involvement of the mediastinal nodes, seven patients were N2, and two were N3. Videothoracoscopy was performed under general anesthesia using a double-lumen endotracheal tube. The videothoracoscope and sonographic probe were inserted via three thoracoports placed in the axillary triangle. RESULTS: Following the results of VOS-USCD, the staging and subsequently the therapeutic program were modified in seven of 10 cases (70%). CONCLUSIONS: Our preliminary experience indicates that VOS-USCD should be applied to the diagnosis of patients in stage IIIA (N2) and that it is particularly valuable for patients in stage IIIB.

Aged↗

Effects of the color additive caramel color III and 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI) on the immune system of rats.

Administration of ammonia caramel color (AC) to rats may decrease blood lymphocyte counts, specifically in rats fed a diet low in vitamin B6. This effect is associated with 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)imidazole (THI). To characterize and compare the effects of AC and THI and to study the influence of dietary pyridoxine, two studies in rats were conducted. Weanling rats fed a diet containing 2-3 ppm pyridoxine and exposed to 4% AC or 5.72 ppm THI in drinking water for 4 weeks showed reduced cell numbers in spleen and popliteal lymph nodes, as well as in the blood. Flow cytometric analyses demonstrated a comparable reduction in B and T lymphocytes. In blood, spleen, and popliteal lymph nodes, CD4+ lymphocytes were more reduced than CD8+ cells. The number of bone marrow cells was not affected. Although thymus weight and cell number were not affected either, a decreased cortex over medulla area ratio and an increase in medullary cell density largely due to an increase in CD4+ thymocytes was observed. Decreased numbers of ED2+ macrophages were observed in the thymic cortex and in the spleen red pulp. All effects observed were either less pronounced or absent in a study with rats fed a diet containing 11-12 ppm pyridoxine. The effects of AC and THI on the immune system were similar. Whereas AC exposure was associated with changes in vitamin B6 status, THI did not induce obvious effects on vitamin B6 parameters. It is proposed that the effects of AC and THI on the immune system are not caused by a disturbance of vitamin B6 metabolism, but may in fact result from a disturbance of a specific PLP-dependent process.

Administration, Oral↗

Simultaneous color constancy: how surface color perception varies with the illuminant.

In two experiments simultaneous color constancy was measured using simulations of illuminated surfaces presented on a CRT monitor. Subjects saw two identical Mondrians side-by-side: one Mondrian rendered under a standard illuminant, the other rendered under one of several test illuminants. The matching field was adjusted under the test illuminant so that it (a) had the same hue, saturation, and brightness (appearance match) or (b) looked as if it were cut from the same piece of paper (surface match) as a test surface under the standard illuminant. Matches were set for three different surface collections. The surface matches showed a much higher level of constancy than the appearance matches. The adjustment in the surface matches was nearly complete in the L and M cone data, and deviations from perfect constancy were mainly due to failures in the adjustment of the S cone signals. Besides this difference in amount of adjustment, the appearance and surface matches showed two major similarities. First, both types of matches were well described by simple parametric models. In particular, a model based on the notion of von Kries adjustment provided a good, although not perfect, description of the data. Second, for both types of matches the illuminant adjustment was largely independent of the surface collection in the image. The two types of matches thus differed only quantitatively, there was no qualitative difference between them.

Color Perception↗

Vena contracta imaged by Doppler color flow mapping predicts the severity of eccentric mitral regurgitation better than color jet area: a chronic animal study.

OBJECTIVES: This study sought to evaluate the relation between the color Doppler-imaged vena contracta and the severity of mitral regurgitation (MR) in a chronic animal model of MR. BACKGROUND: The vena contracta, which is defined as the smallest connection between the laminar flow acceleration zone and the turbulent regurgitant jet, has been reported to be a clinically useful marker for evaluating the severity of valvular regurgitation. METHODS: Six sheep with chronic MR produced by previous operation severing the chordae tendineae were examined. MR jet flows and vena contracta widths were imaged using a Vingmed 775 scanner with a 5-MHz transducer. Image data were directly transferred in digital format to a microcomputer for off-line measurement. MR was quantified as peak and mean regurgitant flow rates, regurgitant stroke volumes and regurgitant fractions determined using mitral and aortic electromagnetic flow probes and flowmeters balanced against each other. RESULTS: Vena contracta width correlated well with regurgitant severity determined by electromagnetic flowmeters (r = 0.95, SEE = 0.05 cm, p < 0.0001 for peak regurgitant flow rate; r = 0.85, SEE = 0.08 cm, p < 0.0001 for regurgitant stroke volume; r = 0.90, SEE = 0.07 cm, p < 0.0001 for regurgitant fraction). CONCLUSIONS: This study shows that the vena contracta width method is useful for predicting the severity of MR. It is simple and conveniently available with high resolution equipment. The quantitative comparisons in the present study lay the foundation for future clinical and research studies using this vena contracta technique.

Animals↗

Comparison of regional myocardial velocities assessed by quantitative 2-dimensional and M-mode color Doppler tissue imaging: influence of the signal-to-noise ratio of color Doppler myocardial images on velocity estimators of the Doppler tissue imaging system.

M-mode color Doppler imaging of the myocardium affords a greater sampling rate and signal-to-noise (S/N) ratio than 2-dimensional (2D) imaging. In this study, we compared myocardial velocities assessed by 2D and M-mode Doppler tissue imaging (DTI) at the same site and evaluated the influence of the S/N ratio on velocity estimates of the currently used DTI systems. In patients with and without impaired regional left ventricular function, myocardial velocities assessed by 2D DTI were lower than those obtained with M-mode DTI. The difference between regional velocities derived from both imaging techniques was positively correlated with the extent of the "black zone," which could be considered as indirectly reflecting the S/N ratio for each frame. Thus in the clinical setting and on currently used echocardiographs, 2D DTI may provide underestimated regional myocardial velocities when compared with M-mode, mainly because of the influence of the lower sampling rate and S/N ratio on velocity estimators of the imaging system.

Echocardiography, Doppler, Color↗

Report on a group form for the measurement of color-word interference: speed of color discrimination test.

Although there have been some suggestions towards the development of a standardized form for a group version of Stroop's color-word interference test yet, to some extent, a group form has not been applied in psychological research. In this study the equivalence of group and individual procedures as well as some standards for psychological tests of the group form were examined. The results show that the interference score does not comply with the requirements of psychological tests and the equivalence of the interference scores of the group and individual procedures could not be determined. For further research experimental analysis of convergent and discriminant validity is suggested.

Adult↗

Reported head injury and sex differences in color naming, color matching, and digit-string matching.

It was hypothesized that the observed sex differences on three speeded tasks could be accounted for in terms of the greater incidence of head injury in men than in women. In two studies of 64 male and 66 female college students significant sex differences were found on digit-string matching and color-matching tasks. When the data from those 39 subjects with reported histories of head injury were eliminated from the analysis or head-injury reports were statistically controlled, significant sex differences remained. Digit-string matching times were significantly correlated with reported head injury in both studies, suggesting that this test is sensitive to residual effects of head injury.

Adult↗

Variability of color choice in the Lüscher Color Test--sex differences.

In this preliminary investigation of variability of responses on the Lüscher Color Test, subjects were 567 volunteers administered the test twice with a 10-min. interval between them. Two hypotheses were tested: (a) that variability of responses attests to the personality of the respondent (tested by the relations of variability and MMPI scale scores), and (b) variability of responses is based on learning the test's content. As such, men's scores should be more variable than women's. Analysis showed that variability was not correlated with scores for personality on the MMPI scales. Men's scores are more variable than women's, but only until age 45.

Adolescent↗

Diagnosis of acute intestinal ischemia by color Doppler sonography. Color Doppler sonography and acute intestinal ischemia.

Detection of acute intestinal ischemia is a diagnostic challenge. Color Doppler sonography of the abdomen may contribute to its diagnosis by showing lesions of the gut wall associated with occlusion or stenosis of the mesenteric vessels. Our overview of the sonographic findings of acute intestinal ischemia is presented as well as a proposition of algorithm for the working up to the diagnosis based on recent clinical experience.

Arterial Occlusive Diseases↗