Effects of fluorescein isothiocyanate labeling on staining characteristics of a fluorescent antibody conjugate for the diagnosis of leptospirosis.
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It is known that the growth inhibitory substance of animal sera on Leptospires exists in the albumin fraction. Since the globulin fraction obtained from animal sera having growth inhibitory property may support, though variable individually, some degree of leptospiral growth, it may be added, with 5% of pooled rabbit serum, to the medium to be used for the propagation of Leptospires.
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