PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Nerve Net”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 343 records · Page 19Linked to original sources
PubMed2006

Mapping the corneal sub-basal nerve plexus in keratoconus by in vivo laser scanning confocal microscopy.

PURPOSE: To produce a two-dimensional reconstruction map of the living corneal sub-basal nerve plexus in keratoconus with in vivo confocal microscopy. METHODS: Four eyes of four subjects with keratoconus were examined by slit lamp biomicroscopy, Orbscan II slit-scanning elevation topography (Bausch & Lomb Surgical, Rochester, NY), and laser scanning in vivo confocal microscopy with the Heidelberg Retina Tomograph II, Rostock Corneal Module (Heidelberg Engineering, Heidelberg, Germany). Subjects were asked to fixate on targets arranged in a grid to enable in vivo confocal microscopy of the cornea in a wide range of positions. RESULTS: A mean of 402 +/- 57 images were obtained for each cornea, to create confluent montages. The mean dimensions of the corneal areas mapped were 6.60 +/- 0.70 mm horizontally and 5.91 +/- 0.72 mm vertically. All corneas exhibited abnormal sub-basal nerve architecture compared with patterns previously observed in normal corneas. At the apex of the cone, a tortuous network of nerve fiber bundles was noted, many of which formed closed loops. At the topographic base of the cone, nerve fiber bundles appeared to follow the contour of the base, with many of the bundles running concentrically in this region. Central sub-basal nerve density was significantly lower in keratoconus corneas (10,478 +/- 2,188 microm/mm2) compared with normal corneas (21,668 +/- 1,411 microm/mm2; Mann-Whitney; P < 0.01). CONCLUSIONS: This is the first study to elucidate the overall distribution of sub-basal nerves in the living central to midperipheral human cornea in keratoconus, using laser scanning in vivo confocal microscopy.

Adult↗
PubMed1993

Neuromuscular organization in the swimming system of the pteropod mollusc Clione limacina.

Swim motor neurons of the pteropod mollusc Clione limacina were identified by a combination of electrophysiological and morphological characteristics. Two types of motor neurons were found, including small motor neurons which are active during both slow and fast swimming and which innervated restricted fields of the ipsilateral wing. General excitor motor neurons have large cell bodies, innervate widespread fields and are recruited into activity for fast swimming. Small motor neurons monosynaptically innervate slow-twitch muscle cells, whereas general excitors monosynaptically innervate both slow-twitch and fast-twitch muscle cells. Activity in general excitors can centrally enhance that in small motor neurons because the neurons are electrically coupled. Neuromuscular recordings and lesion experiments indicate that a peripheral nerve network does not appear to play an important role in the spread of excitation throughout the muscle fields.

Animals↗
PubMed2006

Hypergravity susceptibility of ventral root activity during fictive swimming in tadpoles (Xenopus laevis).

1. Fictive swimming is an experimental model to study early motor development. As vestibular activity also affects the development of spinal motor projections, the present study focused on the question whether in Xenopus laevis tadpoles, the rhythmic activity of spinal ventral roots (VR) during fictive swimming revealed age-dependent modifications after hypergravity exposure. In addition, developmental characteristics for various features of fictive swimming between stages 37/38 and 47 were determined. Parameters of interest were duration of fictive swimming episodes, burst duration, burst frequency (i.e., cycle length), and rostrocaudal delay. 2. Ventral root recordings were performed between developmental stage 37/38, which is directly after hatching and stage 47 when the hind limb buds appear. The location of recording electrodes extended from myotome 4 to 17. 3. Hypergravity exposure by 3 g-centrifugation lasted 9 to 11 days. It started when embryos had just terminated gastrulation (stage 11/19-group), when first rhythmical activity in the ventral roots appeared (stage 24/27-group), and immediately after hatching (stage 37/41-group). Ventral root recordings were taken for 8 days after termination of 3 g-exposure. 4. Between stage 37/38 (hatching) and stage 47 (hind limb bud stage) burst duration, cycle length and rostrocaudal delay recorded between the 10th and 14th postotic myotome increased while episode duration decreased significantly. In tadpoles between stage 37 and 43, the rostrocaudal delay in the proximal tail part was as long as in older tadpoles while in caudal tail parts, it was shorter. During this period of development, there was also an age-dependent progression of burst extension in the proximal tail area that could not be observed between the 10th and 14th myotome. 6. After termination of the 3 g-exposure, the mean burst duration of VR activity increased significantly (p < 0.01) when 3 g-exposure started shortly after gastrulation but not when it started thereafter. Other parameters for VR activity such as cycle length, rostrocaudal delay and episode duration were not affected by this level of hypergravity. 7. It is postulated that (i) functional separation of subunits responsible for intersegmental motor coordination starts shortly after hatching of young tadpoles; and that (ii) gravity exerts a trophic influence on the development of the vestibulospinal system during different periods of embryonic development leading to the formation of more rigid neuronal networks earlier in the spinal than in the ocular projections.

Action Potentials↗
PubMed1995

Allocation of perineuronal nets and parvalbumin-, calbindin-D28k- and glutamic acid decarboxylase-immunoreactivity in the amygdala of the rhesus monkey.

Lattice-like coatings, known as perineuronal nets, were lectin-cytochemically stained with the Wisteria floribunda agglutinin in the lateral nucleus and the intermediate division of the basal nucleus of the amygdala in rhesus monkeys. Perineuronal nets were demonstrated around neurons with parvalbumin- or calbindin-D28k-immunoreactivity, but not around calretinin-containing cells. In parallel dual-peroxidase staining experiments, it was demonstrated that lattice-like coatings exclusively surround GABAergic neurons in this brain region. The novel finding of calbindin-D28k-immunoreactivity in neurons ensheathed by perineuronal nets amplifies the panel of revealed markers in such nerve cells and indicates their cytochemical heterogeneity.

Amygdala↗
PubMed2002

Deficits of temporal discrimination in dystonia are independent from the spatial distance between the loci of tactile stimulation.

To assess whether spatial variables influence deficits of temporal somesthetic discrimination in dystonic patients, 10 patients with idiopathic dystonia and 12 healthy controls were tested with pairs of non-noxious electrical stimuli separated by different time intervals. Stimuli were delivered: (1) to the pad of the index finger (same-point condition), (2) to the pad and to the base of the index finger (same-finger condition), and (3) to the pad of the index and ring fingers (different-finger condition). Subjects were asked to report whether they perceived single or double stimuli in the first condition and synchronous or asynchronous stimuli in the second and third conditions. Somesthetic temporal discrimination thresholds (STDTs) were obtained by computing the shortest time interval at which stimuli, applied to the left or the right hand, were perceived as separate in the first condition or asynchronous in the second and third conditions. STDTs were significantly higher in dystonic patients than controls in all three conditions. In both dystonia patients and controls, STDTs resulted highest in conditions whereby stimuli were maximally separated in space. Results extend current knowledge of deficits of somesthetic temporal discrimination in dystonia by showing that temporal deficits are not influenced by spatial variables.

Adult↗
PubMed2002

Histaminergic modulation of the intact respiratory network of adult mice.

Histaminergic modulation of neuronal activity in the respiratory network was investigated under normoxic and hypoxic conditions in the working heart-brainstem preparation of adult mice. Systemic application of histamine, as well as the H-1 and H-3 receptor agonists 6-[2-(4-imidazolyl)ethylamino]- N-(4-trifluoromethylphenyl) heptanecarboxamide (HTMT) and imetit, 0.5-10 micro M, significantly increased the frequency of respiratory burst discharges. Dimaprit, an H-2 receptor agonist, had no effect on respiratory activity. To test for ongoing histaminergic modulation we applied the histamine receptor antagonists pyrilamine (H-1); cimetidine (H-2) and thioperamide (H-3), each 0.5-10 micro M. Only the H-1 receptor antagonist had significant effects, viz. reduction of respiratory frequency and depression of burst amplitude. Underlying effects of histamine receptor activation were identified at the cellular level. Intracellular recordings showed that histamine mediated an increase in synaptic drive potentials in inspiratory neurones while augmentation of inhibitory and excitatory synaptic activity was observed in expiratory neurones. The augmented synaptic depolarisation of inspiratory neurones was blocked by the H-1 receptor antagonist. Histaminergic modulation is also involved in the hypoxic response of the respiratory network. Blockade of H-1 receptors significantly attenuated secondary depression of the biphasic hypoxic responses, while hypoxic augmentation was not affected. We conclude that histamine is a functional neuromodulator, which is tonically active in the respiratory network and is activated further during hypoxia. The data indicate that histaminergic neuromodulation acts predominantly via H-1 receptors.

Animals↗
PubMed1983

Functional anatomy of the tectum mesencephali of the goldfish. An explorative analysis of the functional implications of the laminar structural organization of the tectum.

The present paper is aimed at an exploration of the possible functional significance of the laminar organization of the goldfish tectum at both the cellular and the synaptic level. For this purpose (1) the data concerning the structure of the teleostean tectum are surveyed, (2) a conceptual framework of the intratectal connectivity in the goldfish is proposed, (3) the electrophysiological data concerning the teleosteam tectum are surveyed and (4) the degree of correlation between the structural and physiological data available is discussed. Apart from the retina, tectal afferents originate from at least 10 other brain centers. At least 5 of these projections appear to be topographically organized. Tectal afferents, neurons as well as synapses reveal a characteristic intratectal lamination pattern. Tectal efferents project to at least 10 brain centers, and have until now been shown to arise from 6 cell types. The structural data surveyed allow the construction of a conceptual framework of tectal circuitry on the basis of 3 starting points. (1) The existence of at least 8 presynaptic zones or laminae, each containing a characteristic set of presynaptic structures (afferents and axons of interneurons). (2) The fact that the tectal postsynaptic structures (somata and dendrites of tectal neurons) each have a characteristic location, extension and synaptic density, which determines the relative importance of the different presynaptic zones for each cell type. (3) The laminar specificity hypothesis, which implies that presynaptic structures that coexist in a particular presynaptic zone terminate without preference on all types of postsynaptic structures within that zone. The conceptual framework of tectal circuitry is quantified in terms of connectivity index and connective importance. Analysis of the framework constructed leads to a detailed description of the intratectal pathways involved in the processing of the 4 main streams of tectal input (i.e. visual, toral, telencephalic and 'deep' input). It was concluded that the laminar organization of the tectum is primarily relevant for multimodal integration and that the tectal cell types each receive a characteristic sample out of the multimodal information available in the different tectal layers.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMedDate not supplied

Hypothalamic and vagal neuropeptide circuitries regulating food intake.

It has been recognized for some time that a number of different neuropeptides exert powerful effects on food intake. During the last few years, the neurocircuitry within which these peptides operate has also begun to be elucidated. Peptidergic feeding-regulatory neurones are found both in the hypothalamus and the brainstem, where they act as input stations for hormonal and gastrointestinal information, respectively. These cell populations both project to several other brain regions and interconnect extensively. The present review summarizes the neuroanatomy and connectivity of some prominent peptides involved in food intake control, including neuropeptide Y, melanocortin peptides, agouti gene-related protein, cocaine- and amphetamine-regulated transcript, orexin/hypocretin, melanin-concentrating hormone and cholecystokinin. Disturbances in the hypothalamic neuropeptide systems have been implicated in the phenotype of a genetic model of fatal hypophagia, the mouse anorexia (anx) mutation, which is also discussed.

Animals↗
PubMed1995

[Immunohistochemical studies of the neuroanatomy of the human turbinates: innervation pattern of seromucous glands].

Seromucous glands are among the main components of human nasal mucosa. To control the different physiological functions of these glands, a dense nerval network is necessary. The aim of this study was to demonstrate the general innervation of the seromucous glands in nasal mucosa. Tissue samples of inferior human turbinates were fixed and embedded in paraffin wax or frozen. Serial sections were performed and incubated with antibodies either to neuron-specific enolase (NSE) or S-100 protein. The ABC method was employed to demonstrate the immunacomplexes. The sections were counterstained with haemotoxylin. Either NSE and S-100 protein-immunoreactive nerve fibres were found around the acini, ducts and in the connective tissue of the glands. Furthermore, a dense network of fine nerve fibres was detected in the submucosal region. The localisation of neurons in nasal glands confirms the direct nerval control of the diverse glandular functions. Additionally, the sensitive subepithelial network of fine nerve fibres might be involved in the regulation of glandular secretion.

Adult↗