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PubMed2003

Incorporation of 14C-palmitic acid into phospholipids in the isolated rat uterus. Effect of exogenous ovarian steroids and gonadotrophin-induced ovulation.

Effect of different hormonal stage on incorporation of (1-14C)palmitic acid (PA) into the uterine phospholipids (PL) has been studied. Virgin female Wistar rats were divided into the following groups: ovariectomized 7 and 28 days, untreated; ovariectomized received a single injection of estradiol, ovarietomized received a single injection of progesterone (after estradiol-priming), intact cycling females received pregnant mares serum gonadotrophin, intact cycling females injected human chorionic gonadotrophin after PMSG-priming. Uteri horns were incubated a buffer containing 14C-PA. Lipids were extracted and fractionated using thin-layer chromatography. Estradiol increased PL-content, incorporation of (1-14C)-PA into the PL, while progesterone inhibited E-dependent effect, Gonadotrophin-induced ovulation provoked increase in uterine PL-content and remained a stimulatory signal for incorporation of (1-14C)-PA.

Animals↗
PubMed2001

Bowman-Birk protease inhibitor and its palmitic acid conjugate prevent 7,12-dimethylbenz[a]anthracene-induced transformation in cultured mouse mammary glands.

The chemopreventive effects of Bowman-Birk protease inhibitor (BBI), a soybean polypeptide, and its palmitic acid conjugate, Pal-BBI, on 7,12-dimethylbenz[a]anthracene (DMBA)-induced transformation were investigated by using an in vitro whole organ culture system of mouse mammary glands. The transformation incidence was measured as the number of glands containing nodule-like alveolar lesions (NLAL). Both BBI and Pal-BBI were effective in preventing DMBA-induced transformation, especially when added to the medium during the developing period after the exposure of mammary glands to DMBA, i.e. 35.9 and 53.4% prevention, respectively. However, when only present in the medium before the promotion period, Pal-BBI was effective in decreasing the transformation incidence (32.2%) while BBI was not (10.3%). This difference could possibly be due to the high lipophilicity and tissue retention of Pal-BBI in mammary gland cultures.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1997

Formula containing randomized fats with palmitic acid (16:0) in the 2-position increases 16:0 in the 2-position of plasma and chylomicron triglycerides in formula-fed piglets to levels approaching those of piglets fed sow's milk.

Human and pig milk fat contains a high proportion of palmitic acid (16:0) which is largely esterified to the 2-position of the triglycerides. In contrast, the 16:0 in most nonmilk fats and in infant formulas is mainly esterified at the triglyceride 1,3 positions. Gastric and pancreatic lipases hydrolyze fatty acids from the dietary triglyceride 1- and 3-positions to produce unesterified fatty acids and 2-monoglycerides which are absorbed and re-esterified. In this study, we determined whether formula with chemically randomized oils, which equally distributes 16:0 among all the positions of triglycerides, influences growth or the distribution of fatty acids in plasma and liver lipid of formula-fed piglets compared with piglets fed formula with native oils or sow's milk. After feeding from birth to 18 d, piglets fed formula with palm olein randomized with canola oil (co-randomized) had higher weight gain per liter of formula intake and higher 16:0 in the chylomicron triglyceride 2-position than piglets fed formula with randomized or native palm olein oil blended with canola oil. The fatty acid distribution of formula triglycerides is an important determinant of pathways of 16:0 absorption, and consequently of plasma lipid fatty acids in formula-fed piglets.

Animals↗
PubMed1997

Fluorescence, polarized fluorescence, and Brewster angle microscopy of palmitic acid and lung surfactant protein B monolayers.

Fluorescence, polarized fluorescence, and Brewster angle microscopy reveal that human lung surfactant protein SP-B and its amino terminus (SP-B[1-25]) alter the phase behavior of palmitic acid monolayers by inhibiting the formation of condensed phases and creating a new fluid protein-rich phase. This fluid phase forms a network that separates condensed phase domains at coexistence and persists to high surface pressures. The network changes the monolayer collapse mechanism from heterogeneous nucleation/growth and fracturing processes to a more homogeneous process through isolating individual condensed phase domains. This results in higher surface pressures at collapse, and monolayers easier to respread on expansion, factors essential to the in vivo function of lung surfactant. The network is stabilized by a low-line tension between the coexisting phases, as confirmed by the observation of extended linear domains, or "stripe" phases, and a Gouy-Chapman analysis of protein-containing monolayers. Comparison of isotherm data and observed morphologies of monolayers containing SP-B(1-25) with those containing the full SP-B sequence show that the shortened peptide retains most of the native activity of the full-length protein, which may lead to cheaper and more effective synthetic replacement formulations.

Amino Acid Sequence↗
PubMed1997

Acylation of human insulin with palmitic acid extends the time action of human insulin in diabetic dogs.

To test whether the binding of insulin to an endogenous serum protein can be used to extend the time action of insulin, human insulin was acylated at the epsilon-amino group of Lys(B29) with palmitic acid to promote binding to serum albumin. Size-exclusion chromatography was used to demonstrate specific binding of the resulting analog, [N(epsilon)-palmitoyl Lys(B29)] human insulin, to serum albumin in vitro, and the time action and activity of the analog were determined in vivo using overnight-fasted, insulin-withdrawn diabetic dogs. In the diabetic animal model, the duration of action of [N(epsilon)-palmitoyl Lys(B29)] human insulin administered intravenously was nearly twice that of unmodified human insulin, and the plasma half-life was nearly sevenfold that of the unmodified protein. Administered subcutaneously, [N(epsilon)-palmitoyl Lys(B29)] human insulin had a longer duration of action; a flatter more basal plasma insulin profile; and a lower intersubject variability of response than the intermediate-acting insulin suspension Humulin L (Lilly, Indianapolis, IN). These studies support the concept that modification of insulin to promote binding to an existing serum protein can be used to extend the time action of human insulin. In addition, the time action, pattern, and decreased variability of response to [N(epsilon)-palmitoyl Lys(B29)] human insulin support the development and further testing of this soluble insulin analog as a basal insulin to increase the safety of intensive insulin therapy.

Acylation↗
PubMed2003

Randomized double-blind study of the nutritional efficacy and bifidogenicity of a new infant formula containing partially hydrolyzed protein, a high beta-palmitic acid level, and nondigestible oligosaccharides.

OBJECTIVES: The aim of this study was to evaluate the nutritional efficacy and bifidogenic characteristics of a new infant formula containing partially hydrolyzed whey protein, modified vegetable oil with a high beta-palmitic acid content, prebiotic oligosaccharides, and starch. METHODS: In a double-blind study, healthy formula-fed term infants aged younger than 2 weeks were randomized to receive either the new infant formula (NF) or a standard formula (SF) until the age of 12 weeks. Anthropometric measurements were taken at enrollment, 6 weeks, and 12 weeks. In a subsample of infants, blood samples were taken at 6 weeks and stool samples were taken at enrollment and 6 weeks. Blood samples were analyzed for biochemical measures of protein status and amino acids, and stools were analyzed for total bacteria and bifidobacteria. Mothers completed a feeding diary and questionnaire at 6 and 10 weeks. RESULTS: One hundred fifty-four infants were enrolled in the study; 102 completed the trial. The growth of infants in both formula groups was in line with published growth curves. During the first 6 weeks, NF girls gained more weight and head circumference than the SF girls. These velocity differences were not maintained throughout the 12-week study period. The NF stools had a higher proportion of bifidobacteria at 6 weeks compared with the SF stools, and they were softer. There were no clinically significant differences in the blood biochemical and amino acid values between groups. Both formulas were well tolerated by the infants. CONCLUSIONS: When compared with a standard infant formula, the new formula supported satisfactory growth, led to higher counts of bifidobacteria in the feces, produced blood bio-chemical values typical of formula-fed infants, and was well tolerated.

Amino Acids↗
PubMed2004

Palmitic acid metabolism in the soleus muscle in vitro in hypo- and hyperthyroid rats.

The aim of this study was to establish whether the rate of fatty acid (FA) incorporation and its utilization by the isolated soleus muscle is modified under conditions of thyroid hormone deficit or excess. The rate of palmitic acid (PA) uptake, oxidation and incorporation into intramuscular lipids with increasing PA concentration (0.5-1.5 mM) in the incubation medium were determined. In hypothyroid rats intramuscular triacylglycerol (TG) synthesis was increased, while the rate of PA oxidation to CO2 and incorporation into mono- and diacylglycerols (MG/DG) and phospholipids (PL) remained unchanged. In rats with triiodothyronine (T3) excess the rate of all processes studied was enhanced, although the percentage incorporation of PA into different classes of intramuscular lipids was fairly constant and, independently of thyroid state and FA concentration in the medium, was 56-66% for TG, 9-14% for MG/DG and 24-32% for PL. Our results thus indicate that even short-term T3 excess accelerates the rate of FA uptake and metabolism in the oxidative soleus muscle, whereas in hypothyroid rats only intramuscular TG synthesis is affected.

Animals↗
PubMed2006

Efflux of sphingolipids metabolically labeled with [1-3H]sphingosine, L-[3- 3H]serine and [9,10- 3H]palmitic acid from normal cells in culture.

The membrane complex lipids of human fibroblasts and differentiated rat cerebellar granule cells in culture were metabolically radiolabeled with [1-(3)H]sphingosine, L-[3-(3)H]serine and [9,10-(3)H]palmitic acid. A relevant efflux of radioactive sphingolipids and phosphatidylcholine was observed from cells to the culture medium in the presence of fetal calf serum. This event was independent of the concentration and structure of the metabolic precursor administered to cells, and it was linearly time-dependent. The radioactive lipid patterns present in the medium were different from those present in the cells. Radioactive sphingomyelin and ganglioside GM3 containing short acyl chains were the main species present in the medium from human fibroblasts, while sphingomyelin and GD3 ganglioside in that from neuronal cells. In the absence of proteins in the culture medium, the efflux of complex lipids was much lower than in the presence of serum, and the patterns of released molecules were again different from those of cells.

Animals↗
PubMed1980

Effect of palmitic acid on regeneration of dichlorovos-inhibited plasma cholinesterase activity in vitro.

Free fatty acids (FFA) in plasma were observed to be significantly increased (p less than 0.001) in high fat diet (HFD) rats and albumin significantly decreased (p less than 0.001) in low protein diet (LPD) rats. Dichlorovos was more toxic to HFD and LPD rats, compared to high protein diet (HPD) and standard diet (SD) rats, but these differences were not statistically significant. The spontaneous regeneration of dichlorovos-inhibited plasma cholinesterase activity in vivo was faster in the HFD rats compared to the other three dietary groups. Adding palmitic acid (PA) in vitro to the plasma system, at PA/albumin molar ratios in the range 0.53:2.83, accelerated spontaneous regeneration of dichlorovos-inhibited plasma cholinesterase activity significantly during 2-24 h periods. PA acceleration of enzyme regeneration rate did not appear to be related to PA interactions with dichlorovos-albumin binding in plasma.

Animals↗
PubMed1999

Quantitative acylcarnitine profiling in fibroblasts using [U-13C] palmitic acid: an improved tool for the diagnosis of fatty acid oxidation defects.

A method was developed for the investigation of mitochondrial fatty acid beta-oxidation in cultured fibroblasts. Monolayer cultures were incubated without foetal calf serum with commercially available [U-13C] palmitic acid and L-carnitine for 96 h. The acylcarnitines produced by the cells were extracted from the cell suspension and analysed either by quantitative stable isotope dilution gas chromatography chemical ionization mass spectrometry, or by fast atom bombardment mass spectrometry. Characteristic acylcarnitine profiles were obtained for all the different enzyme deficiencies investigated, with the exception of carnitine palmitoyltransferase II deficiency and carnitine/acylcarnitine carrier deficiency which showed similar patterns. Comparison between this method and the 3H-myristate and 3H-palmitate tritium release assays revealed that the method described here is superior, allowing unequivocal identification of patients.

Carbon Isotopes↗
PubMed1987

Ligatin: a peripheral membrane protein with covalently bound palmitic acid.

The ligatin monomer is a polypeptide of Mr = 10,000 which is soluble in acidified chloroform:methanol, a characteristic similar to that of Folch-Lee proteolipid. The hydrophobicity of ligatin is also reflected by its ability to interpolate into the phosphatidylcholine bilayer as shown by a concentration-dependent change in membrane conductance. However, unlike other proteolipids the amino acid composition of ligatin is not enriched in hydrophobic amino acids (isoleucine, leucine, valine, methionine, phenylalanine, tryptophan). Instead, the hydrophobic character of ligatin could be explained, at least in part, by the covalent association of fatty acids, 1.4-1.7 mol of palmitate/10,000 g of protein, as revealed by gas chromatography mass spectrographic analyses. The post-translational addition of fatty acid may therefore be the means by which ligatin acquires an affinity for membranes.

Acylation↗