Starch gel electrophoresis of some invertebrate sera.
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Exploitation of newer instrumentation and a dye-binding method for protein measurement made possible reduction in volume down to 1/1000 that commonly used, with no greater error. The procedure was tested at two levels, 1- and 10-microl volumes, with human gamma globulin and rabbit antiserum. Of the dyes tested, bromsulfalein proved best for the protein estimation.
Proteins, certain amino acids, and amines undergo a potentially useful color reaction. The reaction involves the apparent formation of pyrroles when the compounds are allowed to react with acetonylacetone. The pyrroles yield colored complexes on coupling with p-dimethylaminobenzaldehyde. This report describes the specificity and possible uses of this reaction in colorimetric measurements and in paper chromatographic detection of these compounds.
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