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At least 343 records · Page 19Linked to original sources
PubMed1997

Isolation of the trichomonad Tetratrichomonas buttreyi (Hibler et al., 1960) Honigberg, 1963 in bovine diarrhoeic faeces.

A trichomonad was found in the faeces of a heifer with watery diarrhoea. It was classified as Tetratrichomonas buttreyi according to its morphology as revealed by scanning electron microscopy. This flagellate was successfully maintained in a cysteine-peptone-liver medium. It is, to our knowledge, the first report of Tetratrichomonas buttreyi in Spain. This trichomonad appears to be a nonpathogenic commensal which often proliferates in fluid faeces.

Animals↗
PubMed1997

Veterinary parasitology in South Africa: some highlights of the past 100 years.

The reasons for the wide range of parasites occurring in South Africa are mentioned. An account is given of the establishment of veterinary research and training in South Africa. Some major breakthroughs and advances in the study or the control of ecto- and endoparasites as well as protozoal diseases are listed.

Animals↗
PubMed1995

Recent advances in the laboratory diagnosis of equine parasitic diseases.

This article reviews recent advances in laboratory diagnosis of equine parasitic diseases. Laboratory diagnosis of most equine parasitic diseases continues to rely on standard methods. Only laboratory diagnostic tests for EPM, cryptosporidiosis, and giardiasis were included. The criteria for testing and interpretation of results for each new diagnostic method were explained. Western blot and PCR testing for EPM and immunofluorescent staining with monoclonal antibodies for cryptosporidiosis and giardiasis were reviewed.

Animals↗
PubMed1986

Protozoal diseases.

The clinical and pathologic findings of and therapy for such protozoal diseases as equine protozoal myeloencephalitis, toxoplasmosis, sarcocystosis, pneumocytosis, cryptosporidiosis, giardiasis, besnoitiosis, and klossiellosis are discussed. Emphasis is placed on disorders that occur with greater frequency in North America and on emerging protozoal diseases affecting horses.

Animals↗
PubMed1991

Enzyme polymorphism of freshwater fish trypanosomes and its use for strain identification.

Sixteen strains of trypanosomes isolated from the blood of 10 freshwater fish species (all but one from South Bohemia) were characterized by enzyme analysis using disc electrophoresis in 5% polyacrylamide gel. Six enzymes were selected: ADH, LDH, MDH, ME, G6PDH and GPI. The most variable were ADH and LDH, whereas MDH, G6PDH and GPI showed the same pattern in all strains analysed. Cluster analysis indicated a very high degree of similarity among strains, even those isolated from phylogenetically distant hosts. Moreover, no correlation between enzyme pattern and host species was found.

Alcohol Dehydrogenase↗
PubMed1997

Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa.

The taxonomic situation in the genus Tritrichomonas is the subject of controversial discussion: potentially T. foetus and T. suis, the tritrichomonads from cattle and swine, respectively, could belong to the same species. In order to shed some light on this question, a molecular biological analysis was performed. The 5.8S rRNA gene and the flanking internal transcribed spacer regions (ITS1 and ITS2) of 12 different isolates of 3 Tritrichomonas species T. foetus, T. suis and T. mobilensis were enzymatically amplified by PCR and subcloned. Also, the corresponding regions of the trichomonads Trichomonas vaginalis, T. tenax, T. gallinae and Pentatrichomonas hominis were included in this study. Sequence analysis of cloned fragments was used to compare the parasite isolates. The genus Tritrichomonas exhibited an extremely high degree of homogeneity. All T. foetus and T. suis isolates had identical sequences, and only 1 substitution was found in the ITS2 region of T. mobilensis. In contrast, the genus Trichomonas shared more diversity. The results obtained in this study support a possible future revision of the taxonomic classification of tritrichomonads.

Animals↗
PubMed1986

[Discovery of a protist parasite, apparently akin to Acrasiae, in the genital tract and various organs of primates].

Microcysts were first observed in histological sections of the placenta of a gibbon, the uterus of a Microcebus, showing a pyometra, the placenta, spleen and lung of a new born mangabey which died soon after birth from an interstitial pneumonia. Plasmodial forms were also discovered in the sections, either encysted or infiltrating the tissues. Cultures from all the above mentioned pathological material were attempted on a special medium, the composition of which will be given later. All cultures shew myxamoeba-like organisms aggregating and merging into pseudoplasmodial forms that produced microcysts. Attention is drawn to the pathogenic rôle of this new kind of parasites in order that they should be searched for, in every case of genital troubles not ascribable to other causes.

Animals↗
PubMedDate not supplied

Tritrichomonas foetus: a scanning electron microscopy study of erythrocyte adhesion associated with hemolytic activity.

The in vitro hemolytic activity of Tritrichomonas foetus was investigated. The parasite was tested against human erythrocytes of groups A, B, AB, and O, and against erythrocytes of nine adult animals of different species (the rabbit, rat, chicken, cat, dog, swine, horse, bovine, and sheep). The results showed that T. foetus strains (ATCC KV1, K, PAL, 5022, RJ, 90) did not present any hemolytic activity against any human erythrocyte group nor against rabbit, rat, chicken, cat, dog and swine erythrocytes. T. foetus strains, however, lysed horse, bovine, and sheep erythrocytes. No hemolysin released by the parasites could be identified. Hemolysis did not occur with trichomonad culture supernatants, with sonicated extracts of T. foetus, nor with killed organisms. Scanning electron microscopy (SEM) showed that human erythrocytes did not adhere to the trophozoites, in contrast horse erythrocytes adhered to the surface of the parasites and were phagocytosed for up to 90 min. The parasites are able to exert their cytopathic effects through: (a) physical contact established between the two cell surfaces, (b) toxins released from parasites into the interaction media, or (c) the association of both mechanisms. Further studies are necessary to clarify the importance of the hemolytic activity in the biology of T. foetus.

Animals↗
PubMed1989

Parasite survey of eight wild animals in the Ruma National Park.

Eight game animals representing seven species in the Ruma National Park in South Nyanza, Kenya, were examined for the presence of blood protozoa, ectoparasites, and helminthic and coccidian endoparasites using standard parasite-identification methods. Haematological parameters were also determined. The oribi was positive for Trypanosoma brucei ssp. and the reedbuck for T. congolense. No other blood protozoans were found. Strongyle eggs were found in the faeces of all species except the water buck. Five of eight animals harboured liver flukes and five were parasitized by ticks of the genus Amblyomma. One roan antelope was anaemic, but the other animals had haemoglobin levels within the normal range and appeared to be in a good state of health.

Animals↗
PubMed1975

Hexamitiasis in cadmium-exposed mice.

Mortality was observed in 4- to 5-week-old Swiss Webster mice exposed to 300 or 3 ppm cadmium as cadmium chloride in the drinking water. Mice receiving 300 ppm cadium suffered 26% mortality as compared with 7% of those on the low cadmium dose. Death did not occur in control mice. Clinical signs and histopathology established Hexamita muris as the causative agent. Cadmium lesions were not observed. It is suggested that mortality due to hexamitiasis resulted from synergism between cadmium and H muris.

Animals↗