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Renal cysts in pigs: prevalence and pathology in slaughtered pigs from a single herd.

An abattoir investigation into kidney condemnations due to renal cysts in pork pigs from one producer showed a prevalence of 47.5 per cent. Affected pigs were the progeny of a landrace boar. Similar cysts found in a small sample of day-old piglets sired by the same boar indicated a congenital origin. A marked variation in size and frequency of cysts in the kidneys of slaughtered pigs was seen, some resembling the severity of polycystic kidneys. Histologically cysts appeared to be of tubular origin. The prevalence of affected pigs in slaughter groups from the single herd could be genetically determined. If this were so the data would be consistent with autosomal dominant inheritance. A breeding experiment to examine this hypothesis is in progress.

Animals↗

Procedures for preventing the transmission of foot-and-mouth disease virus to pigs and sheep by personnel in contact with infected pigs.

The most effective method of containing an outbreak of foot-and-mouth disease (FMD) is by the culling of livestock. However, qualified people must diagnose the disease before the culling can begin, and they must avoid susceptible animals after having been in contact with infected premises, to prevent them from transmitting the virus. To test the effectiveness of biosecurity procedures in preventing the transmission of FMD virus (O/UK/35/2001) investigators contacted and sampled pigs inoculated with FMD virus for approximately 45 minutes and then contacted and sampled sentinel pigs and sheep after either using no biosecurity procedures, or washing their hands and donning clean outerwear, or showering and donning clean outerwear. The virus was detected in the nasal secretions of one investigator immediately after the postmortem investigation of the inoculated pigs but was not detected in samples collected between approximately 12 and 84 hours later. After the contaminated personnel had showered and changed into clean outerwear they did not transmit the strain of FMD virus to susceptible pigs and sheep.

Animal Husbandry↗

The effect of feeding various levels of Bifidobacterium globosum A on the performance, gastrointestinal measurements, and immunity of weanling pigs and on the performance and carcass measurements of growing-finishing pigs.

Four trials using 312 weanling pigs (average initial weight, 7.2 kg) were conducted to examine the effect of Bifidobacterium globosum A (BGA) on the growth performance, scour scores, humoral and cell-mediated immune response, and pH and chloride ion concentration (CIC) of feces and gastrointestinal tract contents of pigs. Dietary treatments were 0, 5.0 x 10(4), 6.7 x 10(6), and 7.5 x 10(8) colony forming units (cfu) of BGA/d in Trial 1 and 0, 6.0 x 10(4), 5.0 x 10(5), and 5.0 x 10(6) cfu/d in Trials 2 through 4. Pigs fed the low or medium levels of supplemental BGA had a greater ADG (P < .05) than control pigs throughout Trial 1, whereas ADG was quadratically increased (P < .05) for the low or medium levels only during wk 3 to 5 for the pooled data for Trials 2, 3, and 4. The primary effect of dietary BGA additions on ADFI was a quadratic increase during wk 3 to 5 for Trial 1 (P < .05) and for the pooled data of Trials 2 through 4 (P < .10). Gain:feed ratios were generally unaffected by addition of BGA. Both ADFI and ADG tended to be decreased (P < .10) at the highest level of BGA in all trials. Scouring was not severe in any of the trials and was not consistently affected by feeding BGA. The pH and CIC of gastrointestinal contents or feces were not influenced by the feeding of BGA.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

A modification to the isotope-dilution technique for estimating milk intake of pigs using pig serum.

The aim of this study was to compare the measurement of deuterium oxide (D2O) directly in pig serum with that in sublimed whole blood. This was to assess whether excluding vacuum sublimation before analysis would cause any significant loss of accuracy in estimates of pig milk intake. Water and serum standards were made in deionized water and serum, respectively, and were assayed with samples under the same conditions on a fixed-filter, infrared spectrophotometer. The mean concentration of D2O in sublimed samples was 2,244 microg/mL of body water, and the mean concentration of D2O in serum samples was 2,184 microg/mL of body water. The mean ratio of D2O concentration in deionized water to the D2O concentration in serum was 1.0275, which was used as a correction factor to convert serum D2O concentration to D2O concentrations in body water. Using this method, the mean concentration of D2O in all serum samples was identical to that in sublimed samples (i.e., 2,244 microg/mL of body water). Mean milk intake of pigs based on sublimed samples was 1,006 g/d and that based on serum samples was 1,012 g/d. This confirms that milk intake determined from measurement of D2O directly in pig serum is sufficiently precise.

Animals↗

Energy value of pig feeds: effect of pig body weight and energy evaluation system.

Ad libitum energy intake and performance in pigs depends on many animal and environmental factors in which feed energy density plays an important role. In addition, feed represents an important cost in pig production, and energy represents the greatest proportion of this cost. It is therefore important to express the feed energy value on an appropriate basis, and both energy supply (a dietary characteristic) and energy requirement (an animal characteristic) should be expressed using the same system. Energy content depends first on the nutrient composition; the constituents differ markedly in GE content (23.0, 39.0, 17.4, and 18.4 kJ/g for CP, fat, starch, and dietary fiber, respectively). Due to differences in digestibility and associated endogenous energy losses, the actual contribution of nutrients to apparent DE supply in growing pigs is even more variable and ranges from 31.7 kJ/g for fat, 22.4 kJ/g for CP, 17.2 kJ/g for starch, to only 3.2 kJ/g for dietary fiber. Nutrient composition also affects the efficiency of conversion of ME to NE, which varies from 90% for fat to 82% for starch and 60% for CP. Consequently, the energy values (relative to a conventional diet containing 14.2, 13.6, and 10.3 MJ/kg of DE, ME, and NE, respectively) of corn, soybean meal, and animal fat are 100, 104, and 235 on a DE basis; 102, 99, and 244 on a ME basis; and 107, 79, and 289 on a NE basis. Energy value thus depends on the system of evaluation. The energy density of pig feeds can also be affected by feed processing. For example, pelleting markedly increases fat and energy digestibilities in corn or full-fat rapeseed. Also the animal itself can affect the energy value of nutrients; digestion of dietary fiber becomes more efficient with increasing BW, with subsequent differences in energy content of feeds according to BW. In conclusion, a satisfactory characterization of the energy value of feeds should be based on their NE content. Factors affecting nutrients digestibility (e.g., BW and feed processing) should also be taken into account.

Animal Feed↗

Characterization of antimicrobial-resistant phenotypes and genotypes among Salmonella enterica recovered from pigs on farms, from transport trucks, and from pigs after slaughter.

The main objectives of this study were to determine antimicrobial resistance patterns among Salmonella serotypes and to evaluate the role of transport trucks in dissemination of antimicrobial-resistant strains of Salmonella. Salmonella from groups of nursery and finishing pigs on farms, from trucks, and from pigs after slaughter were compared using serotyping, patterns of antimicrobial resistance, and pulsed-field gel electrophoresis patterns. The five farms included in the study yielded 858 isolates representing 27 Salmonella serovars. The most common resistance observed (80% of all isolates) was to tetracycline; resistance to ampicillin (42%), chloramphenicol (31%), amoxicillin/clavulanic acid (30%), and piperacillin (31%) also were common. We found a correlation between serovar and antimicrobial resistance. High correlation was found between Salmonella Typhimurium var. Copenhagen and chloramphenicol resistance (Spearman rank correlation, rho = 0.7). Multidrug resistance was observed primarily in Salmonella Typhimurium var. Copenhagen (94%) and Salmonella Typhimurium (93%) and was much less common in the other common serovars, including Salmonella Derby (7%) and Salmonella Heidelberg (8%). Of the 225 isolates exhibiting the most common pentaresistance pattern in this study, amoxicillin/clavulanic acid-ampicillin-chloramphenicol-piperacillin-tetracycline, 220 (98%) were Salmonella Typhimurium var. Copenhagen, and 86% of the isolates of this serovar had this pattern. Isolates from the trucks were similar, based on pulsed-field gel electrophoresis patterns, to those from the cecum and mesenteric lymph nodes of pigs on two of the farms, suggesting the probable infection of pigs during transport. Class I integrons were also common among various serovars.

Animals↗

[Improved pig health with farm management systems. Project 'Beautiful stream of pigs' 21].

As part of the project 'Clean pigs', an inventory was made of the different pig farm management systems described in the literature. These systems were evaluated for their potential use in improving animal health, focusing on the pathogens important in the Netherlands. The most promising systems for the control and/or eradication of pig pathogens in the Netherlands can be divided into two groups (with decreasing effect): I. Eradication from an existing positive population. The best systems for this are Embryo Transplantation (ET), Specific-Pathogen Free pigs (SPF), Piglet Snatching and Segregated Weaning (SW). II. Strategic veterinary and breeding strategies. The best systems for this are Freeze infection, test and removal, Test and removal, Vaccination, Medical elimination, Strategic medication and Genetic resistance. Farm systems can be a big help when trying to improve animal health, but the 'biosecurity' measures on the farm are at least of equal importance.

Animal Husbandry↗

The effect of outdoor production on the seroprevalence of Lawsonia intracellularis in growing-finishing pigs in a large pig production unit infected with endemic porcine proliferative enteropathy.

The study was performed in a large Croatian production unit from May 2000 till June 2002. Blood samples form late-pregnant gilts were tested by indirect immunofluorescence antibody (IFA) serum assay for Lawsonia intracellularis. The offspring of 301 positively tested gilts were dislocated after the nursery phase either to indoor or outdoor growing-finishing facilities. Ten percent of these animals (142 indoor, 143 outdoor raised pigs) were tested at 2, 6, 10, 14, 18, 22 and 26 weeks of age for seroprevalence of Lawsonia intracellularis. All offspring of IFA positive gilts were seronegative at 2 and 6 weeks of age. At 10 weeks of age 71.1% (101 animals) of indoor and 32.8% (47 animals) of outdoor pigs were tested positive (P < 0.05). While at 14 weeks of age 71.1% of indoor raised pigs showed seropositivity, the seropositivity declined in outdoor units to 7.6% (P < 0.01). At weeks 18 (52.1%), 22 (47.8%) and 26 (21.7%) indoor raised pigs still showed marked seropositivity and but their outdoor raised counterparts returned to seronegativity.

Animal Husbandry↗

Development of gastric proteases in fetal pigs and pigs from birth to thirty six days of age. The effect of adrenocorticotropin (ACTH).

Development of the synthesis and secretion of gastric proteases was studied in 55 Large White x Landrace pigs from 22 days before birth (93 days gestation) to 36 days of age. The pigs came from eight litters and were 0.4 - 13.5 kg body weight. Littermate pairs were treated with either saline or adrenocorticotropin (ACTH) from three days of age. Secretion of protease activity (milk-clotting and general proteolytic activity) was investigated in anaesthetized pigs by a gastric perfusion technique using intravenous infusion of pentagastrin at dose rates of 4 and 8 micrograms/h per kg body weight. In addition, concentrations of protease zymogens (prochymosin, pepsinogen A, progastricsin) were measured in fundic tissue extracts by rocket immunoelectrophoresis. Prochymosin was present in fundic tissue at 22 days before birth, reached peak concentrations at birth and decreased in concentration during the subsequent 36 days. Pepsinogen A and progastricsin were absent or present in trace amounts in the first week after birth, but thereafter concentrations of both zymogens increased rapidly. Development of the pentagastrin-stimulated secretion of protease activity reflected the changes of zymogen concentrations in fundic tissue. Chronic treatment of pigs with ACTH from three days of age significantly increased the concentration of prochymosin in fundic tissue at 9-11 days and the concentrations of pepsinogen A and progastricsin at 34-36 days of age. Hormones such as ACTH and glucocorticoids may therefore play a regulatory role in the ontogeny of porcine gastric proteases.

Adrenocorticotropic Hormone↗

Form and development of the umbilical cord in pigs and their association with delivery of viable pigs.

Length, extensibility, and absolute strength of umbilical cords were determined in litters of pigs at term. Length was also determined in fetuses earlier in gestation and in hypophysectomized fetuses. Mean cord length at term was 34.6 cm (range, 17 to 50 cm) and its length was correlated with length and birth weight of pigs. Cords were significantly (P less than 0.01) shorter in hypophysectomized fetuses, but cord length was not influenced by sex, position in the uterus, or twisting of the cord. Although the umbilical cord was capable of being stretched considerably, the degree varied among pigs. Tension required to break portions of the cord ranged from 545 to 2,000 g. Variations in length, elasticity, and strength of the cord may influence safe delivery, birth asphyxiation, and perinatal mortality in pigs.

Animals↗

[Hygienic aspects of pig's head meat. 2. Mechanical separation of pigs' heads].

Deboning pigs' heads is a labour-intensive process. According to information supplied by the industry, mechanical separation of pigs' heads offers good prospects. Data on the hygienic implications of this process were not known. These were studied more closely in an experimental design. The bacteriological and sensory qualities as well as the chemical and histological composition of the product were compared with those of manually obtained pig's head meat. In addition, the yield of the process of separation was determined. Three groups of heads were mechanically separated, viz. untreated heads and heads which had previously been dipped in water having an initial temperature of 100 degrees C for one minute and five minutes respectively. These studies served to verify the fact that mechanical separation of pigs' heads is technically possible. Previous dipping of the heads in water at a temperature of 100 degrees C produced a considerable improvement of the yield in meat, which was due to setting of the rind. The chemical composition of the product was roughly identical with that of manually obtained head meat. However, tissue composition and structure of the tissues differed markedly. An important drawback is the higher count of Enterobacteriaceae in the product. These bacteria are probably released on compression from sites which are difficult of access such as the nose, the pharyngeal cavity and the alveoli. In addition, they may be possibly protected by a covering layer of mucus. Therefore, mechanical separation is not regarded as acceptable from the point of view of hygiene.

Animals↗

Purification and properties of a pig heart thiolase with broad chain length specificity and comparison of thiolases from pig heart and Escherichia coli.

A thiolase (acetyl CoA acyltransferase, EC 2.3-1.16) which acts on substrates of various chain lengths (thiolase I) has been purified from pig heart muscle 366-fold to near homogeneity as judged by gel electrophoresis. Its molecular weight was estimated to be 200,000 in the absence and 46,000 in the presence of sodium dodecyl sulfate. Kinetic measurements with acetoacetyl coenzyme A, 3-ketohexanoyl-CoA, 3-ketooctanoyl-CoA, and 3-ketodecanoyl-CoA yielded apparent Km values of 16, 8.3, 2.4, and 1.8 micron, respectively, whereas apparent Vmax values of 65 to 69 mumol/min/mg were obtained with all substrates except for acetoacetyl-CoA, with which a value of 26.5 mumol/min/mg was observed. Antibodies prepared against this thiolase were used to demonstrate that thiolase I and acetoacetyl-CoA thilase (thiolase II) from pig heart mitochondria are immunologically unrelated. The antibodies cross-reacted, however, with thiolase I from beef heart. Kinetic constants (Km, Vmax) were also determined for thiolases I and II from Escherichia coli, as were the native and subunit molecular weights of E. coli thiolase II. Although the E. coli thiolases were found to be immunologically distinct from the pig heart enzymes, their physical and kinetic properties are strikingly similar to those of the heart thiolases. In view of this finding and in view of the known physiological functions of the E. coli thiolases, it is proposed that thiolase I from pig heart is only involved in fatty acid metabolism, whereas thiolase II functions solely in ketone body degradation.

Acetyl-CoA C-Acyltransferase↗

Rosette-formation of pig thymic lymphocytes with sheep and pig erythrocytes. II. Markers for cortical and medullary thymocytes.

Study of the distribution of P+S+ and P-S+ cells in various lymphoid organs of pig revealed that the thymus contains the highest proportion of P+S+ cells (75.5 +/- 4.2%). In vivo treatment of neonatal piglets with cortisone: (i) Reduced both the area of the thymic cortex even more than the medulla and the P+S+ cells more than the P-S+ cells, suggesting the respective origin of these two populations in these two tissue compartments. Moreover, cortisone-resistant thymocytes responded better than the control untreated pig thymocytes to Con A and PHA stimulation. (ii) Reduced the number of P+S+ cells in peripheral organs. Separation of adult thymocytes into P+S+ and P-S+ cell fractions showed that P-S+ cells responded better than P+S+ to Con A and PHA stimulation. Cortisone-resistant thymocytes and P-S+ cells showed the same cell size distribution and both are enriched in medium sized cells. Thus, it is concluded that the property of rosette formation with pig red blood cells allows the identification of two subsets of pig thymocytes with different biological properties and thymic locations, which by analogy with other species may prove to have different patterns of emigration to peripheral organs.

Animals↗

[Showering of slaughtering pigs. I. A prospective study of the preference of slaughtering pigs for showering after transport and its consequences for meat quality].

In the Netherlands it is common practice to shower pigs awaiting slaughter during the two-hour period of rest in lairage. However, the quantity of water used in showering is considerable. There is a tendency to restrict the use of water for this purpose. Water, even if the well from which it is drawn is owned, is an expense, as taxes designed to cover the cost of preventing water-pollution are based on the quantity of water used. The main question asked in slaughter houses is whether spraying a mist of water is as effective as is showering (sprinkling drops of water). Another question is whether intermittent showering could replace continuous showering. The length of the periods of showering and intervals between these periods are of importance. So is the temperature of the water. By approaching these questions through two disciplines: applied ethology (preference-tests and general observations) and meat-technology (recording carcass quality), an effort was made to find an answer. Sprinkling obviously did not offer any advantage as regards the pigs or the carcass quality, when it was compared with spraying a mist of water, and uses five times as much water during a similar period and on the same surface. The pigs preferred four showers, each lasting ten minutes, to continuous showering throughout the two hours in lairage. Carcass quality was not adversely affected by intermittent showering compared with continuous showering. The experiments were carried out during a (cool) summer period. The pigs did not show any preference for lukewarm water rather than cold. Naturally, the response could be entirely different in winter time.(ABSTRACT TRUNCATED AT 250 WORDS)

Abattoirs↗

Molecular cloning of cDNA for guinea pig CYP1A2 comparison with guinea pig CYP1A1.

Guinea pig CYP1A2 cDNA was isolated by RT-PCR from liver tissue of 3,3'-methylcholanthrene-treated guinea pigs. It shares considerable sequence identity with guinea pig CYP1A1 (nt 77%, aa 65%), but differs in levels of constitutive expression, function, and inducibility. Western blot analysis of protein expressed by full-length cDNA in COS-1 cells identified CYP1A2 (56 kDa) and CYP1A1 (53 kDa) proteins in corun liver microsomes. CYP1A2 transfectants metabolized methoxyresorufin and ethoxyresorufin, while CYP1A1 transfectants metabolized only ethoxyresorufin. Constitutive expression of CYP1A2 mRNA (2.0 kb) and protein was much lower than that of CYP1A1 mRNA (2.6 kb) and protein, but the fold induction of CYP1A2 by 3,3'-methylcholanthrene was greater than that of CYP1A1. Changes in splicing of CYP1A2 pre-mRNA occur upon treatment with 3,3'-methylcholanthrene.

Amino Acid Sequence↗

Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig.

In this study we extracted guinea pig brain and testis with; the extract was adsorbed to and eluted from cartridges (the Sep-Pak C18 procedure). We found this procedure superior for recovering crystalline insulin added to buffers or tissues, and for recovering endogenous insulin from plasma, but inferior for recovery of insulin from tissues. However, we did find 'rat/pork' type-insulin in guinea pig brain and testis (5-50 pg/g wet weight tissue). Our results with the Sep-Pak C18 procedures were reproduced by four other laboratories (who found 4-60 pg/g wet weight of tissue) and similar findings were also obtained by an independent investigator. Thus, we conclude that extrapancreatic tissues of guinea pigs have a second type of insulin-related material that is more typical of other mammalian insulins, but that the amount recovered is dependent upon the extraction procedure utilized.

Animals↗

Ultrastructural development and persistence of guinea pig cytomegalovirus in duet cells of guinea pig submaxillary gland.

Salivary glands from Hartley guinea pigs were experimentally infected with guinea pig cytomegalovirus (GPCMV) and examined by light and electron microscopy at different time intervals. Characteristic intranuclear and intracytoplasmic viral inclusions were observed in duct cells of infected animals. Viral inclusion counts and infectivity titers in the salivary gland reached maximum levels by 3 to 4 weeks after infection; infectivity persisted, though at reduced levels, for at least 30 weeks. Electron microscopic examination of viral inclusions revealed several developmental events including nucleocapsid assembly, envelopment of nucleocapsids at the inner nuclear membrane and their enclosure by a thin vacuolar membrane. While contained within cytoplasmic vacuoles, enveloped virions acquired surface spikes. Cytoplasmic vacuoles containing virions subsequently coalesced and discharged mature virions at the cell surface into the lumen of the salivary gland duct. The data indicate that the ultrastructural development of GPCMV in the guinea pig salivary gland shows many similarities to that of human cytomegalovirus in humans. The salivary gland may provide a primary locus for virus shedding and horizontal transmission of cytomegalovirus.

Animals↗

H1-receptor reserves in guinea-pig left atria, trachea and pig coronary artery as identified by phenoxybenzamine.

H1-receptor reserves in guinea-pig left atria, trachea and pig coronary arteries were calculated by the use of phenoxybenzamine (Pba), a beta-haloalkylamine that irreversibly blocks the H1-receptor response to histamine or 2-(2-pyridyl)-ethylamine (PEA). Equieffective concentrations of the H1-agonist in the absence (A) and presence (A') of Pba were evaluated from concentration-response curves. By plotting the reciprocal values 1/A versus 1/A' the amount of H1-receptors not occupied by the agonist was calculated. The size of the H1-receptor reserve could be estimated by comparison of the receptor occupation with the corresponding effect. Furthermore, the dissociation constants for histamine, PEA, Pba and the pD'2-values for Pba were determined for the different tissues. 5% and 6% H1-receptor occupation is necessary to achieve a half maximal contraction of the trachea with the agonists histamine and PEA, respectively. Only 0.5% H1-receptor occupation is needed for the half maximal positive inotropic effect of histamine in left atria, while 5.5% of the H1-receptors have to be occupied using PEA as an agonist in this tissue. In the coronary artery of the pig 50% of the maximal contraction can be achieved by stimulation of 15.1% of the H1-receptors with histamine.

Animals↗