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At least 343 records · Page 19Linked to original sources
PubMed1995

Preferential selection of VP7 gene from a parent rotavirus strain (SA11) in sequential passages after mixed infection with SA11 and SA11-human rotavirus single-VP7 gene-substitution reassortants.

We studied the competitive growth among SA11-L2(G3) and its single-human VP7 gene-substitution reassortants SA11-L2/KU-R1(G1) and SA11-L2/DS1-R1(G2), which have the genetic background of SA11-L2, during sequential passages after mixed infection. When the same infectious units (m.o.i. of 5 p.f.u./cell) of SA11-L2 and a reassortant SA11-L2/KU-R1 were inoculated onto and passaged in MA104 cells, 88% of the virus clones isolated from the culture fluid at the 3rd passage belonged to G3, and all the clones from the 10th passage had G3 specificity. Even when SA11-L2/KU-R1 with titer 10 times higher than that of SA11-L2 was used in the coinfection, the predominance of clones with G3-VP7 was observed. Although G2 clones slightly surpassed G1 clones in number in the mixed culture of SA11-L2/KU-R1 and SA11-L2/DS1-R1, G3 clones predominated in the virus progeny from a mixed culture infected with the same titers of SA11-L2, SA11-L2/KU-R1, and SA11-L2/DS1-R1. However, no significant difference in viral growth was detected among SA11-L2 and the two reassortants.

Antigens, Viral↗
PubMed1993

The avian rotavirus Ty-1 Vp7 nucleotide and deduced amino acid sequences differ significantly from those of Ch-2 rotavirus.

The Vp7 gene of the avian strain Ty-1, which is classified as G7 serotype, was sequenced and the amino acid sequence deduced. The gene is 1065 nucleotides long with a long open reading frame of 987 nucleotides producing a protein 329 amino acids in length. The amino acid homology of the Ty-1 Vp7 protein to that of the avian Ch-2 Vp7 was 70%. The A, B, and C variable epitope regions of Ty-1 were unique compared to those of Ch-2 and other strains representing the 14G serotypes. The low 53% homology of the A and C regions of Ty-1 and Ch-2 would suggest that Ty-1 may be of a different serotype to the G7 reference strain Ch-2.

Amino Acid Sequence↗