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In vivo rehardening of enamel eroded by a cola drink.

There are many concerns about the erosive effects of acidic beverages. In this study, the effects of Pepsi-Cola (pH 2.41) on bovine enamel and the rehardening effect resulting from intraoral exposure of the teeth were determined by microhardness testing (Vicker's Hardness Number). Bovine enamel specimens (VHN 380.00 +/- 12.74) were immersed in 100 ml of Pepsi-Cola for five minutes and subsequently exposed to human intraoral environment through the use of a removable resin plate. Microhardness testing was performed on specimens after one hour, twenty-four hours and forty-eight hours of exposure to an intraoral environment. The microhardness value was significantly (p < 0.05) reduced by the cola beverage (VHN 262.13 +/- 20.34), and significantly (p < 0.05) increased after intraoral exposure for one hour (VHN 299.75 +/- 26.86) and twenty-four hours (VHN 328.00 +/- 18.70). The difference in the microhardness between the twenty-four-hour group and the forty-eight-hour group (VHN 333.50 +/- 15.13) was not significant (p > 0.05). The microhardness value of the forty-eight-hour group was significantly less than the values recorded during the initial pre-study measurements p < 0.05).

Acids↗

Release of cariostatic agents from a new buffering fluoride- and xylitol-containing lozenge to human whole saliva in vivo.

A new buffering lozenge (sucking tablet) was developed for patients susceptible to dental caries and erosion, in particular for those with reduced salivary secretion. As active ingredients this lozenge comprises of a combination of xylitol, fluoride, calcium, phosphate, zinc and buffering compounds. To test the lozenge's activity in vivo, the release of ingredients was monitored in 19 healthy subjects for 22 min after sucking the lozenge was completed. In subjects with a normal salivary secretion rate the lozenge caused only a slight stimulation of saliva flow, but a significant elevation both in salivary pH and buffer effect was observed. Furthermore, fluoride, calcium and phosphate were effectively released into whole saliva with peak values 2-4 min after use. The same salivary parameters were also quantitated after 1 month's regular use (3 lozenges/day) but no consistent long-term changes were found. Salivary mutans streptococci and total anaerobic microflora did not change significantly during the long-term use. The results show that the buffering fluoride- and xylitol-containing lozenge, which also releases calcium and phosphate, is active in vivo but its serviceability as a remineralizing agent, in particular for elderly patients with reduced salivary flow rate, has to be analysed separately.

Administration, Oral↗

Abrasion of eroded root dentine brushed with different toothpastes.

This study evaluated the surface roughness change and wear provided by different dentifrices on root dentine previously exposed to erosive challenges. According to a randomized complete block design, 150 slabs of bovine root dentine (6 x 3 x 2 mm) were ground flat and polished. In an area of 4 x 3 mm on the dentine surface, specimens were submitted to five erosive/abrasive events, each one composed by: exposure to Sprite Diet or distilled water for 5 min, then to a remineralizing solution for 1 min, and simulation of 5,000 brushing strokes. Four dentifrices--regular (RE), baking soda (BS), whitening (WT) and tartar control (TC)--and distilled water (CO), used as control, were compared. Final texture and the wear depth were evaluated using a profilometer. ANOVA did not show significant interaction, indicating that the effect of dentifrices on both surface roughness change and wear did not depend on whether or not the dentine was eroded ( p>0.05). There was no difference between abrasion of eroded and sound dentine. The Tukey's test revealed that WT, BS and TC provided the highest increase in surface roughness differing from RE and CO. TC yielded the deepest wear of root dentine, whereas RE and CO, the shallowest. No significant difference in wear among BS, TC and WT were observed. Within the limitations of this study, the data showed that abrasion of both eroded and sound root dentine was dependent on the dentifrice used.

Analysis of Variance↗

Susceptibility of enamel surfaces to demineralization after application of fluoridated carbamide peroxide gels.

The aim of the present study was to evaluate the effect of experimental, fluoridated carbamide peroxide gels on formation of erosively induced demineralization of enamel. Seventy-five bovine enamel specimens were polished for microhardness determination and evenly distributed among 5 groups (A-E). The specimens were treated with 10% carbamide peroxide gel (8 h) and subjected twice to remineralization for 2 h in artificial saliva and demineralisation for 90 s in 1% citric acid, pH 2.2. The cycles of treatment with carbamide peroxide and twofold re- and demineralization were repeated three times. The carbamide peroxide gels were different in pH and fluoride content. Gel A (pH 7.0) and gel B (pH 5.7) were fluoridated (0.5% F), gel C (pH 7.0) and gel D (pH 5.7) were not fluoridated. In control group E the samples were not treated with a gel, but stored in 100% humidity for 8 h instead. Knoop microhardness of the specimens was assessed directly after polishing, and after each carbamide peroxide treatment and demineralization. All specimens showed a loss of microhardness at the end of the experiment. After 3 days, the controls revealed a significantly lower hardness loss compared to the specimens treated with the carbamide peroxide gels. Surface softening was significantly lower for the specimens of group A compared to the remaining groups (B-D), which were not significantly different among each other. It is concluded that treatment with either fluoridated or unfluoridated carbamide peroxide gels, at either neutral and acidic pH, renders enamel more susceptible to demineralization. Use of a fluoridated neutral gel decreases the degree of surface softening compared to the other gels investigated.

Analysis of Variance↗

Pronamel and tooth mousse: an initial assessment of erosion prevention in vitro.

OBJECTIVES: The aim of this study was to examine whether a single topical application of proenamel or tooth mousse would prevent enamel erosion METHODS: Enamel samples were treated with either proenamel or tooth mousse applied for 15 min. The control group was placed in distilled water for 15 min. All specimens were then exposed to an erosive challenge of 0.2% citric acid for 1h. Enamel loss was determined using surfometry. RESULTS: The mean amount of enamel removed in the control group was 5.02 microm (S.D. 1.16). The mean enamel loss in the proenamel group was 2.60 microm (S.D. 0.90) and the mean enamel loss in the tooth mousse group was 3.28 microm (S.D. 1.22). The results for the proenamel group were statistically significantly different from the control group at the p<0.01 level and the results for the tooth mousse group were statistically significantly different from the control group at the p<0.05 level. CONCLUSIONS: Tooth mousse and proenamel may offer a degree of protection from erosion of permanent enamel.

Analysis of Variance↗

Effect of a casein/calcium phosphate-containing tooth cream and fluoride on enamel erosion in vitro.

The aim of this study was to determine whether a tooth cream containing casein/calcium phosphate (CasCP) protects enamel against erosion. Sixty bovine enamel specimens were prepared for profilometry and distributed into five groups. Specimens were rinsed with artificial saliva interrupted by 1% citric acid (pH 2.3) for 30 s 6 times daily for 14 days. Group 1 (n=12) was not treated (control); in group 2 (n=12) CasCP was applied for 120 s twice daily; in group 3 (n=12) 250 ppm fluoride as NaF was applied for 120 s twice daily; in group 4 (n=12) CasCP was applied for 120 s, then 250 ppm fluoride for 120 s twice daily, and in group 5 (n=12) amine fluoride (AmF) gel (12,500 ppm fluoride) was applied for 120 s twice daily. Differences between groups with respect to erosive enamel loss (profilometrically determined depth after 7 and 14 days) were tested by the Mann-Whitney test (alpha=0.05). After 7/14 days' erosive cycling, specimens treated with AmF gel showed significantly less enamel loss (18.5/35.5 microm; medians) than those treated with CasCP (25.5/46.9 microm), 250 ppm fluoride (25.0/ 40.9 microm), CasCP and 250 ppm fluoride (23.9/47.4 microm) or with no treatment (26.3/49.8 microm). It is concluded that highly fluoridated acidic AmF gel can protect enamel against erosion while CasCP, 250 ppm fluoride or a combination of CasCP and 250 ppm fluoride provide little protection.

Amines↗

Measurement of tooth wear in patients with palatal erosion.

AIM: To develop a reproducible method for the assessment of tooth wear in vivo using a laser profilometer. DESIGN: A controlled study to measure tooth wear. SUBJECTS AND METHODS: Wear was measured over a 6-month period in 13 patients with unexplained palatal dental erosion and compared with a group of 7 controls without any evidence of abnormal wear. MAIN OUTCOME MEASURES: Metal disks were cemented to the tooth surface and impressions taken at 6-month intervals. Wear was estimated by scanning the impressions with a contacting laser profilometer and measuring a change in depth around the disk over a 6-month period using fixed reference points on the metal disks. RESULTS: A statistically significant difference was observed between patients with palatal erosion and the controls. Patients with erosion had a median of 36.5 microns of wear over 6 months (range 17.6-108.2) and the controls had a median of 3.7 microns (range 0.5-15.8). CONCLUSIONS: This paper presents a novel method for measuring erosion using fixed reference points cemented to the palatal surfaces of upper incisor teeth.

Adolescent↗

The acidic and erosive potential of five sports drinks.

Sports drinks are becoming increasingly popular as we are all being encouraged to adopt a healthier lifestyle with regular exercise. However, many of these products are based on acidic fruits and may contribute to erosion. The aim of this study was to screen a number of these products for their potential to cause enamel erosion in vitro. The erosive potential of a number of readily available sports drinks was assessed in the laboratory by measuring their pH, neutralisable acidity and their ability to erode enamel. These were compared to a positive control, orange juice and a negative control, water. The pH of the sports drinks ranged from 3.16-3.70 with their neutralisable acidity ranging from 9.74-13.44 mls of 0.1M NaOH. The amount of enamel removed following 1-hour immersion in the sports drinks ranged from 1.18-5.36 microns. In comparison, the orange juice control had a pH of 3.68, a neutralisable acidity of 19.68 mls of 0.1 M NaOH and removed 3.24 microns of enamel. Many of the sports drinks tested were found to be erosive. This information will be of use to clinicians when counselling patients with tooth surface loss who use fruit based sports drinks regularly.

Beverages↗