SEARCH · PubMed Health

Results for “data accessibility”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 343 records · Page 19Linked to original sources

PubMedDate not supplied

AIDA for reproductive medicine and the fertility clinic.

A Medical Information System for the Fertility Department has been built (VERGYNIA) using the 4th generation software package AIDA to make the medical data accessible for research and teaching on the one hand and to assist the management of the department in the daily routine of patient care on the other. The system has been implemented on a PDP 11/23 computer with a 20 Mbyte hard disk, a 10 Mbyte removable disk and 128 Kbyte central memory. Three visual display terminals and a small printer are connected to the system. A dedicated line connection between this system and the computer facilities of the Department of Medical Informatics allows easy transfer of data for analysis by statistical packages and the transfer of new programs to the computer of the Fertility Department. The PDP 11/23 contains a production system, a developmental system, and a separate environment for research which can all three run simultaneously on the same computer without interfering with each other. VERGYNIA was predominantly constructed with AIDA; only parts of the output programs have been programmed in MUMPS, due to the fact that the required AIDA tools were not yet available at the time of development. The original version of VERGYNIA was already in operation in 1982 and was built with the tools that form the basis of the current AIDA release. Due to many ad hoc modifications and additions, the system needed a total redesign in order to make it compatible with the new enhancements of the current AIDA release. This redesign was carried out during 1985.

Ambulatory Care Information Systems↗

PubMed2003

CADB: Conformation Angles DataBase of proteins.

Conformation Angles DataBase (CADB) provides an online resource to access data on conformation angles (both main-chain and side-chain) of protein structures in two data sets corresponding to 25% and 90% sequence identity between any two proteins, available in the Protein Data Bank. In addition, the database contains the necessary crystallographic parameters. The package has several flexible options and display facilities to visualize the main-chain and side-chain conformation angles for a particular amino acid residue. The package can also be used to study the interrelationship between the main-chain and side-chain conformation angles. A web based JAVA graphics interface has been deployed to display the user interested information on the client machine. The database is being updated at regular intervals and can be accessed over the World Wide Web interface at the following URL: http://144.16.71.148/cadb/.

Animals↗

PubMed2003

Data use and information creation: challenges for marine scientists and for managers.

In the coastal waters of European countries and in the offshore waters of the north-east Atlantic, there is an increasing need for scientists to meet challenging objectives, such as to identify meaningful measures of 'quality', and to recommend 'indicators' to underpin implementation of directives, conventions, statutes and other more informal national and international initiatives. Those indicators may relate to particular species or habitats, to changes in physical and chemical characteristics, and even to the use to which the system is put. The problems to be overcome are difficult, but new and developing approaches will make a significant contribution. The approaches include: criteria to identify 'sensitivity' and 'importance', structures to organise information and electronic information resources to access data. The real challenge is to make the results of the various scientific initiatives relevant to and understandable by a wide range of customers with similar overlapping requirements, and thus make a genuine contribution to protecting the marine environment. Above and beyond that is the need for scientists to drive the agenda to enable real and lasting progress to be made towards ecosystem-based management of our seas and a proper consideration of what 'sustainability' may mean in the marine environment and how we utilise its resources.

Conservation of Natural Resources↗

PubMed1997

Real-time telediagnosis of radiological images through an asynchronous transfer mode network: the ARTeMeD project.

The ARTeMeD project aims to solve problems of interactivity and real-time in teleradiology. It integrates personal multimedia facilities and patient data access in a common platform that allows radiologists to collaborate from remote sites through a suitable communication support. ARTeMeD is based on asynchronous transfer mode (ATM) network technology and an optimized manipulation of medical image material. The ARTeMeD system opens interesting perspectives in the area of collaborative teleradiology.

Computer Systems↗

PubMed2001

Inpatient costs of major cardiovascular events.

OBJECTIVE: Many trials of new therapies for cardiovascular disease include economic measures to assess the impact of treatment on healthcare costs, however, it is difficult to compare results between trials due to variation in methods for assigning costs. Therefore we developed a standard library of inpatient hospital costs for major cardiovascular events commonly reported in trials for new cardiovascular therapies. DESIGN: Mean and median hospital charges for each event were calculated from Medicare admissions selected by ICD-9-CM codes from the most recent Healthcare Cost and Utilisation Project (HCUP) Nationwide Inpatient Sample (NIS) database available. Charges were converted to costs using the cost-to-charge ratio from the most recent Medicare cost report data and updated to 1999 using a model derived from the Medicare Payment Advisory Commission (MedPAC) forecast to recommend annual updates to Medicare. RESULTS: Total hospital costs for medical events ranged from $US3654 (1999 values) to $US7833; total hospital costs for surgery and procedures ranged from $US7054 to $US46 317. The distribution of hospital costs is skewed with median costs and lengths of stay lower than mean values. Costs for patients who died in the hospital were generally higher than costs for patients who were discharged. CONCLUSIONS: The library of costs was calculated using a uniform method based on publicly available and easily accessible data and may be updated from year to year. This method provides standardised estimates of hospital costs that can be used in economic analyses of cardiovascular clinical trials.

Cardiovascular Diseases↗

PubMed2005

A case for manual entry of structured, coded laboratory data from multiple sources into an ambulatory electronic health record.

Laboratory results provide necessary information for the management of ambulatory patients. To realize the benefits of an electronic health record (EHR) and coded laboratory data (e.g., decision support and improved data access and display), results from laboratories that are external to the health care enterprise need to be integrated with internal results. We describe the development and clinical impact of integrating external results into the EHR at Intermountain Health Care (IHC). During 2004, over 14,000 external laboratory results for 128 liver transplant patients were added to the EHR. The results were used to generate computerized alerts that assisted clinicians with managing laboratory tests in the ambulatory setting. The external results were sent from 85 different facilities and can now be viewed in the EHR integrated with IHC results. We encountered regulatory, logistic, economic, and data quality issues that should be of interest to others developing similar applications.

Ambulatory Care Information Systems↗

PubMed1991

Medical network security and viruses.

Medical network as connecting Hospital Information Systems are needed in order to exchange, compare and make accessible data. The use of OSI standard communication protocols (open-network environment) will allow to interconnect multiple vendor systems and to accommodate a wide range of underlaying of communication technologies. The security of information on a given host may become dependent of the security measures employed by the network and by other hosts. Computer viruses modifies the executable code and thrive in network environment filled with personal computers and third-party software. Most networks and computers, permit users to share files; this, let the viruses to bypass the security mechanisms of almost every commercial operating system. However, computer viruses axes not the only threat to the information in a network environment. Other as deliberate (passive attacks -wire-tapping-) and accidental threat (unauthorized access to the information) are potential risks to the security information. Cryptographic techniques that now are widely used can resolve the external security problems of the network and improve the internal security ones. This paper begins describing the threats to security that arise in an open-network environment, and goes to establish the security requirements of medical communication networks. This is followed by a description of security services as: confidentiality, integrity, authentication, access control, etc., that will be provided to include security mechanisms in such network. The integration of these security mechanisms into the communication protocols allows to implement secure communication systems that not only must provide the adequate security, but also must minimize the impact of security on other features as for example the efficiency. The remainder of the paper describes how the security mechanisms are formed using current cryptographic facilities as algorithms, one-way functions, cryptographic systems (symmetric and asymmetric), etc. Emphasis is placed on the method to obtain these mechanisms. We will obtain several mechanisms of varying strength for the provision of each security service. Finally, the security mechanisms are structured into several mutually related areas of network security and are presented in a formal form.

Computer Communication Networks↗

PubMed2025

Beacon Reconstruction Attack: Reconstruction of genomes in genomic data-sharing beacons using summary statistics.

MOTIVATION: Genomic data-sharing beacon protocol, developed by the Global Alliance for Genomics and Health, offers a privacy-preserving mechanism for querying genomic datasets while restricting direct data access. Despite their design, beacons remain vulnerable to privacy attacks. This study introduces a novel privacy vulnerability of the protocol: one can reconstruct large portions of the genomes of all beacon participants by only using the summary statistics reported by the protocol. RESULTS: We introduce a novel optimization-based algorithm that leverages beacon responses and SNP correlations for reconstruction. By optimizing for the SNP correlations and allele frequencies, the proposed approach achieves genome reconstruction with a substantially higher F1-score (70%) compared to baseline methods (45%) on beacons generated using individuals from the HapMap and OpenSNP datasets. We show that reconstructed genomes can be used by downstream applications such as in membership inference attacks against other beacons. Our findings reveal that beacons releasing allele frequencies substantially increase the reconstruction risk, underscoring the need for enhanced privacy-preserving mechanisms to protect genomic data. AVAILABILITY AND IMPLEMENTATION: Our implementation is available at https://github.com/ASAP-Bilkent/Beacon-Reconstruction-Attack.

Genomics↗

PubMed1996

Depiction of infarct frequency distribution by computer-assisted image mapping in rat brains with middle cerebral artery occlusion. Comparison of photothrombotic and intraluminal suture models.

BACKGROUND AND PURPOSE: Histopathologic analysis of experimental brain damage has traditionally been performed by measuring areas of infarction and/or selective neuronal alterations on a section-by-section basis in individual animals. For series containing multiple replicate animals, quantitation of tissue injury is typically performed at similar coronal levels throughout an experimental group. A means of facilitating pictorial group comparisons of these histopathologic alterations between different series of replicate studies is highly desirable. METHODS: We introduce a newly designed approach to achieve this goal, based on a linear affine transformation that is used to map corresponding sections at the same anatomic level into a common template to yield a frequency distribution map depicting the aggregate data set. We have applied this approach to compare the histopathologic features of two models of middle cerebral artery (MCA) occlusion in rats: (1) photothrombotically induced permanent distal MCA occlusion in spontaneously hypertensive rats (SHR) and (2) temporary MCA occlusion by intraluminal suture in Wistar rats. RESULTS: The brains of SHR rats with permanent distal MCA occlusion showed a high frequency of infarction involving the dorsolateral and lateral portions of the ipsilateral neocortex, whereas Wistar rats with 90-minute MCA suture occlusion showed a zone of infarction largely concentrated in the dorsolateral portion of the ipsilateral caudoputamen. Infarct frequency distributions for the two animal groups were compared statistically at three corresponding anatomic levels by Fisher's exact test; the resulting statistical parametric maps are shown. CONCLUSIONS: With the use of frequency distribution maps, the pattern of trends within a group can be observed coronally or three-dimensionally. One can directly access data as to numbers of rats with infarction for any point on the map. Studies performed under different experimental conditions can also be compared with one another by means of the generated data sets.

Animals↗

PubMed1990

Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3.

When used to probe EcoRI-digested Candida albicans DNA, the moderately repetitive sequence Ca3 generated a Southern blot hybridization pattern which included 15 to 25 bands, depending upon the strain. The pattern was stable through 400 generations in each of three independent strains but variable between most of 46 unrelated tester strains, making it a very effective probe for discrimination between strains. Computer-assisted methods (Dendron) were developed for storage of Ca3 patterns in data files, calculation of similarity (SAB) values between strains based upon band positions and intensities, and generation of histograms and dendrograms based on SAB values for all strains or any subset of strains in large epidemiological studies. In testing the effectiveness of the system, it was found that (i) multiple isolates from different body locations of the same healthy individual could represent either the same strain or different strains, (ii) isolates from oral lesions of a husband and wife represented the same strain, (iii) strains isolated from the mouths of 10 healthy individuals on the same day and in the same geographical location were as dissimilar on average as the 46 unrelated tester strains, and (iv) strains isolated from seven immunocompromised patients hospitalized over a 2.5-month period in the same hospital were highly similar, indicating nosocomial origin. The apparent effectiveness of these fingerprinting methods and the Dendron program suggests that interlaboratory procedures for fingerprinting should be standardized and all patterns should be analyzed and stored in a common and accessible data base for broad epidemiological analysis.

Blotting, Southern↗

PubMed2004

Anisotropic small amplitude Peptide plane dynamics in proteins from residual dipolar couplings.

The effect of small amplitude anisotropic peptide plane motion on residual dipolar couplings (RDC) measured in proteins has been investigated. RDC averaging effects in the presence of GAF (Gaussian axial fluctuation) motions are found to vary strongly depending on the peptide plane orientation. Even low amplitude dynamics can significantly affect derived alignment tensor parameters if this motion is not taken into account. An analytical description of averaged N-(N)H RDCs is introduced that includes basic GAF-like motion. The averaging depends on the orientation of the peptide plane (alpha, beta, gamma) in the alignment frame and on the motional amplitude (sigma). This expression is used to investigate the presence of anisotropic reorientational dynamics in proteins by incorporating sigma as an additional parameter into the alignment tensor analysis. Average GAF amplitudes (sigma(av)) are determined for secondary structural elements from single experimental N-(N)H RDC data sets from five different proteins, in combination with high-resolution structural models. This yields statistically significant improvement over the static description, and detects sigma(av) values ranging from 14.4 to 17.0 degrees for the different proteins. A higher value of sigma(av) = 20 degrees from loop regions was found using two independent sets of N-(N)H RDC in the protein lysozyme, for which a very high-resolution structure is available. Comparison of fitting behavior over 13 structures from lysozyme of crystal diffraction resolution ranging from 0.9 to 2.1A indicates a small spread of motional amplitudes, demonstrating that the method is robust up to this level of resolution. A combined definition of (alpha)C-C' and N-(N)H RDC under the influence of GAF motions allows simultaneous fitting of both RDC. Application to three proteins leads to similar sigma(av) values and a more significant improvement with respect to the static model. Using the GAF model to describe conformationally averaged RDC is important for two reasons: a more accurate definition of the alignment tensor magnitude can be derived, and the method can be used to detect average small amplitude motions in protein backbones from readily accessible data, on time scales not easily sampled by other NMR techniques.

Anisotropy↗

PubMed2004

Psychiatric correlates of gambling in adolescents and young adults grouped by age at gambling onset.

BACKGROUND: Gambling is a prevalent behavior, yet few studies have investigated its mental health correlates. Although early-onset engagement in behaviors with addictive potential has generally been associated with more severe problems, direct investigation of a nationally representative sample of gamblers grouped by age at onset of gambling has not been performed. OBJECTIVE: To identify differences in psychiatric correlates of gambling and gambling-related attitudes and behaviors in adolescents (aged 16-17 years) and in young adults (aged 18-29 years) with early-onset (before age 18 years) and adult-onset gambling. DESIGN: Logistic regression analysis. SETTING: Public access data set derived from random-digit-dialing telephone surveys. PATIENTS: The study analyzed data from adolescent (n = 235), early-onset adult (n = 151), and adult-onset (n = 204) past-year gamblers and adolescent (n = 299) and adult (n = 187) nongamblers in the Gambling Impact and Behavior Study. MAIN OUTCOME MEASURES: Gamblers and nongamblers were compared within each group on measures of sociodemographics and psychiatric health. Adolescent, early-onset adult, and adult-onset past-year gamblers were compared on measures of gambling attitudes and behaviors. RESULTS: Adolescent gamblers were more likely than adolescent nongamblers to report alcohol and drug use and abuse/dependence and depression. Elevated rates of alcohol and drug use and abuse/dependence were observed in early-onset adult gamblers vs adult nongamblers, and only elevated rates of alcohol use were observed in adult-onset gamblers vs adult nongamblers. Substantial differences in reasons for and patterns of gambling were observed among the 3 groups of gamblers. CONCLUSIONS: Adolescent-onset gambling is associated with more severe psychiatric problems, particularly substance use disorders, in adolescents and young adults. More research is needed to investigate the relationships and inform prevention and treatment strategies.

Adolescent↗

PubMedDate not supplied

Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database.

The HeLa cell line, a human adenocarcinoma, is used in many research fields, since it can be infected with a wide range of viruses and intracellular bacteria. Therefore, the mapping of HeLa cell proteins is useful for the investigation of parasite host cell interactions. Because of the recent improvements of two-dimensional gel electrophoresis with immobilized pH gradients (IPG) compared to isoelectric focusing with carrier ampholytes, a highly reproducible method for examining global changes in HeLa cell protein expression due to different stimuli is now available. Therefore, we have initiated the mapping of [35S]methionine/cysteine-labeled HeLa cell proteins with the 2-D PAGE (IPG)-system, using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and N-terminal sequencing for protein identification. To date 21 proteins have been identified and mapped. In order to make these and future data accessible for interlaboratory comparison, we constructed a 2-D PAGE database on the World Wide Web.

Acrylic Resins↗

PubMed1991

Activity-withdrawal, diagnosis, and demographics as predictors of premorbid adjustment.

This study addressed the need for the establishment of clinically viable "easy access" data that could help predict outcome during the earliest stages of hospitalization. The study examined the contributions of an activity-withdrawal rating, a DSM-III diagnosis, and demographic variables to the prediction of prognosis. The subject population consisted of 152 patients drawn from the population of three psychiatric units. Prognosis was predicted successfully by activity-withdrawal, diagnosis, and demographic variables; the best estimate of prognosis resulted from the combination of all the predictor variables. Results are discussed in terms of the individual and joint contributions of the independent variables to prognostic prediction as well as the probable reasons for differential prognosis for each predictor category.

Adult↗

PubMed2004

Public health monitoring of developmental disabilities with a focus on the autism spectrum disorders.

Developmental disabilities (DDs) are conditions characterized by physical, cognitive, psychological, sensory, adaptive, and/or communication impairments manifested during development. Approximately 17% of individuals in the United States 18 years and younger have a DD, and for most children the cause of their condition is unknown. Of particular interest are the autism spectrum disorders (ASDs), characterized by unusual social, communication, and behavioral development. Previously autism was thought to be a rare condition, but the number of children receiving services for an ASD has increased dramatically in the last decade. Concerns about increases in DDs, particularly ASDs, their causes, and the high costs of intervention have highlighted the need for systematic public health monitoring. Service provider data, such as annual reporting of special education services or of state DD programs, do not provide a complete estimate of the rates for DDs, including ASDs. Unlike genetic metabolic disorders or congenital hearing loss (HL) for which newborn screening programs can provide accurate prevalence rates, there are currently no genetic or biologic markers for the ASDs to enable consistent and early identification of affected children. Centers for Disease Control and Prevention's (CDC) Metropolitan Atlanta Developmental Disabilities Surveillance Program (MADDSP) is a model for population monitoring of ASDs/DDs that has been implemented in other states. This article discusses the role of ASD/DD tracking in public health, as well as the challenges of ASD/DD tracking, including case definition and identification, associated conditions, linkages, and data access.

Autistic Disorder↗

PubMed2001

On-line estimation of the metabolic burden resulting from the synthesis of plasmid-encoded and heat-shock proteins by monitoring respiratory energy generation.

Human basic fibroblast growth factor (hFGF-2) was produced in high-cell density cultures of recombinant Escherichia coli using a temperature-inducible expression system. The synthesis rates of proteins were followed by two-dimensional gel electrophoresis of the (35)S-methionine-labeled proteom. After temperature induction of hFGF-2 synthesis, the rate of total protein synthesis per biomass increased by a factor of three, mainly as a result of the additional synthesis of hFGF-2 and heat-shock proteins. The synthesis rates of heat-shock proteins and constitutive plasmid-encoded proteins increased after the temperature upshift also in the control strain without hFGF-2 gene but followed time profiles different from the producing strain. The energy demand for the extra synthesis of plasmid-encoded and heat-shock proteins resulted in an elevated respiratory activity and, consequently, in a reduction of the growth rate and the biomass yield. A procedure was developed to relate the energy demand for the additional synthesis of these proteins to the generation of energy in the respiratory pathway. Specific energy production was estimated based on on-line measurable rates of oxygen consumption, or carbondioxide evolution and growth, respectively. In this way, the metabolic burden resulting from the synthesis of plasmid-encoded and heat-shock proteins was quantified from on-line accessible data.

Electrophoresis, Gel, Two-Dimensional↗

PubMed2004

Use of information resources by patients with cancer and their companions.

BACKGROUND: Use of the Internet is common among patients with cancer and their companions. However, little is known about patterns of use of print or telephone-based resources amidst growing Internet utilization, nor is it known whether different types of information are sought from electronic compared with print media. It is not clear as to whether patients and their companions differ in their patterns of content seeking. METHODS: A survey was developed to evaluate the use of electronic and nonelectronic informational resources by patients and their companions. During a 10-week period, this questionnaire was administered to 443 outpatients and 124 paired companions attending an urban academic cancer center. RESULTS: In this cohort, 64% of patients and 76% of companions were computer owners, with home Internet access indicated by 58% and 68%, respectively. Use of the Internet to obtain cancer-related information was reported by 44% of patients and 60% of companions. Print resources were used by 79% of patients and 83% of companions, with telephone resources used by 22% and 23%, respectively. The majority of Internet users also read print content (85%), whereas one-half of print users did not access data electronically (52%). Topic areas sought via print and the Internet were similar, with the exception of nutrition-related information, which was more commonly sought in print texts. There was a high rate of concordance between patient and companion use of both electronic and nonelectronic resources. CONCLUSIONS: Despite recent publicity and scrutiny focusing on the quality of Internet health care content, print products remain the most common source of information sought by patients with cancer. Future investigation should focus on the quality of print products used by patients.

Adult↗

PubMed1992

Constraints imposed by protease accessibility on the trans-membrane and surface topography of the colicin E1 ion channel.

The surface topography of a 190-residue COOH-terminal colicin E1 channel peptide (NH2-Met 333-Ile 522-COOH) bound to uniformly sized 0.2-micron liposomes was probed by accessibility of the peptide to proteases in order (1) to determine whether the channel structure contains trans-membrane segments in addition to the four alpha-helices previously identified and (2) to discriminate between different topographical possibilities for the surface-bound state. An unfolded surface-bound state is indicated by increased trypsin susceptibility of the bound peptide relative to that of the peptide in aqueous solution. The peptide is bound tightly to the membrane surface with Kd < 10(-7) M. The NH2-terminal 50 residues of the membrane-bound peptide are unbound or loosely bound as indicated by their accessibility to proteases, in contrast with the COOH-terminal 140 residues, which are almost protease inaccessible. The general protease accessibility of the NH2-terminal segment Ala 336-Lys 382 excludes any model for the closed channel state that would include trans-membrane helices on the NH2-terminal side of Lys 382. Lys 381-Lys 382 is a major site for protease cleavage of the surface-bound channel peptide. A site for proteinase K cleavage just upstream of the amphiphilic gating hairpin (K420-K461) implies the presence of a surface-exposed segment in this region. These protease accessibility data indicate that it is unlikely that there are any alpha-helices on the NH2-terminal side of the gating hairpin K420-K461 that are inserted into the membrane in the absence of a membrane potential. A model for the topography of an unfolded monomeric surface-bound intermediate of the colicin channel domain, including a trans-membrane hydrophobic helical hairpin and two or three long surface-bound helices, is proposed.

Amino Acid Sequence↗