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PubMed1987

Uptake of channel catfish virus from water by channel catfish and bluegills.

Radiolabeled channel catfish virus entered the gills of juvenile channel catfish (Ictalurus punctatus) and was then concentrated in the gut and the liver over 48 hours. Diminution of radioactivity was not detected in these tissues over the course of the experiment. Bluegills (Lepomis macrochirus) were capable of clearing the virus during a period of 48 hours. [3H]Thymidine alone had a different distribution in the channel catfish than did labeled virus.

Animals↗
PubMed1996

Effects of sperm concentration and egg number on fertilization efficiency with channel catfish (Ictalurus punctatus) eggs and blue catfish (I. furcatus) spermatozoa.

The mean sperm concentration of 10 blue catfish (Ictalurus furcatus ) was 1.03 x 10(10) per gram of testis. Testis weighed 3.9 and 17.2 g, with a mean of 6.6 g per fish. Fertilization rate of channel catfish (Ictalurus punctatus ) eggs fertilized with 5.00 x 10(4) to 1.20 x 10(7) blue catfish spermatozoa per egg was 17 to 87%, with an overall mean of 65%. Sperm concentrations of 5.0 x 10(4)/egg exhibited a lower, 16.6% (P < 0.05) fertilization rate than higher sperm concentrations (1.25 x 10(5) to 1.20 x 10(8)/egg). Batches of 450, 2,000, 5,000, 8,000 and 11,000 eggs were similarly fertilized with various sperm concentrations. Mean fertility rate ranged from 25 to 67%, with an overall mean of 53%. The largest egg mass produced the lowest (P < 0.05) fertilization rate. A combination of 450 eggs per batch and 5.0 x 10(5) to 1.20 x (8) sperm per egg produced the highest rate of fertilization (67 to 87%).

Journal Article↗
PubMedDate not supplied

Channel catfish, Ictalurus punctatus rafinesque, neutrophil adhesion to selected extracellular matrix proteins, lipopolysaccharide, and catfish serum.

Adhesion of leukocytes to endothelium and extracellular matrix proteins is an important step in the inflammatory process. Therefore, the adhesion of channel catfish neutrophils to a surface coated with extracellular matrix proteins, LPS, and non-immune catfish serum was evaluated. Stimulation of neutrophils with phorbol dibutyrate (PDBU) resulted in at least two-fold increases in cellular adhesion to all substrates tested except laminin. When EDTA was included during or after PDBU stimulation, neutrophil adhesion to fibrinogen and LPS coated surfaces was reduced to the level of unstimulated neutrophils or to 50-60% of that for stimulated neutrophils. Similarly, EDTA and Ca2+/Mg2+ deficient medium reduced homotypic aggregation of PDBU stimulated neutrophils to background levels. Adhesion of stimulated neutrophils to fibrinogen coated surfaces was inhibited 44, 33, and 50% when soluble fibrinogen, fibronectin, and serum, respectively, were used to block the adhesion assay. The tripeptide integrin adhesion recognition sequence, Arg-Gly-Asp (RGD), caused 83% reduction and the fibrinogen-binding inhibitor protein caused 10% reduction in binding of stimulated neutrophils to fibrinogen coated surfaces. Two hexapeptides tested did not reduce neutrophil adhesion to fibrinogen. The binding of channel catfish neutrophils to the matrices used in the present study is suggestive that integrin mediated adhesion occurs during biological and pathological processes of teleosts.

Animals↗
PubMed1980

Channel catfish virus: comparative replication and sensitivity of cell lines from channel catfish ovary and the brown bullhead.

A cell line derived from channel catfish ovary tissue was compared with the brown bullhead (BB) cell line for their respective abilities to replicate and detect channel catfish virus (CCV). The channel catfish ovary cell line (CCO) produced cytopathic effects (CPE) more rapidly and detected CCV at higher dilutions than did the BB cell line. Production of CCV was more rapid in CCO cells than in BB cells, but the peak titers of the two lines were not significantly different. The CCO cell line was shown to be the more sensitive cell line for CCV research and diagnostics.

Animals↗
PubMed1998

Testosterone accelerates the development of the catfish GnRH system in the brain of immature African catfish (Clarias gariepinus).

The effects of two endogenous steroids on the maturation of the catfish GnRH and the chicken GnRH-II system in the African catfish were investigated. Immature fish (2 weeks of age, which is before sexual differentiation; thus male and female genotypes present) were fed with food pellets containing either testosterone (T), 11beta-hydroxyandrostenedione (OHA) or no steroid (control). After 2 and 4 weeks of treatment, the effects on the two GnRH systems were investigated immunocytochemically, using specific antibodies against the respective GnRH-associated peptides. By means of fluorescence microscopy the number of GnRH perikarya and the cell surfaces were determined. Confocal laser scanning microscopy was applied to verify spatial distribution and staining intensity. After 2 weeks of treatment no difference in any of the parameters between the groups was observed. However, 4 weeks T treatment resulted in significantly more cfGnRH-ir perikarya in the brain compared to the OHA and control groups. In addition, in the T group the number of immunoreactive fibers was markedly higher and the staining of the perikarya and axons was more intense. The distribution of cfGnRH-ir neurons over the ventral forebrain differed between the two age groups: in 4-week-old fish, the largest concentration of neurons was localized in the ventral telencephalon, while 2 weeks later the number of neurons in the supraoptic area had markedly increased, suggesting that the cfGnRH system is still undergoing developmental changes during this period. In 6-week-old fish the average volume of the cfGnRH perikarya (expressed as surface size in the microscopical sections) in both the OHA and the T group was significantly bigger than that in the control group. The cGnRH-II-ir neurons in the midbrain tegmentum showed strong immunoreactivity in all groups, both treated and nontreated. In contrast to the cfGnRH neurons, the staining intensity and the number of cGnRH-II neurons did not change after steroid treatment. The results of this study show that T is able to accelerate the development of the cfGnRH system, whereas OHA has only minimal effects; the cGnRH-II system develops independent from these steroids.

Animals↗
PubMed1987

Trimethylarsine oxide in estuary catfish (Cnidoglanis macrocephalus) and school whiting (Sillago bassensis) after oral administration of sodium arsenate; and as a natural component of estuary catfish.

Oral administration of sodium arsenate to estuary catfish (Cnidoglanis macrocephalus) and school whiting (Sillago bassensis) resulted in an accumulation of trimethylarsine oxide in their tissues. The levels of arsenobetaine, which occurs naturally in these fish, did not appear to be affected by the oral dosing with sodium arsenate. Trimethylarsine oxide also occurred as a natural component of estuary catfish and its presence may be related to the mode of feeding of this fish.

Administration, Oral↗
PubMed1997

Killing of Edwardsiella ictaluri by macrophages from channel catfish immune and susceptible to enteric septicemia of catfish.

The role of peritoneal macrophages in immunity to enteric septicemia of catfish (ESC) after infection with live Edwardsiella ictaluri was investigated. Channel catfish macrophage-mediated bacteriocidal activity was dependent on the macrophage:bacteria ratio. Ratios of 1:1 to 1:12 exhibited significant differences (P < or = 0.05) in killing between macrophages from immune fish when compared to killing by macrophages from susceptible fish at 2.5 h. At 5 h, macrophages from immune fish were capable of effective killing (83.3%) at a 1:24 effector:target ratio, whereas macrophages from susceptible fish killed significantly (P < or = 0.05) less (56.9%). Macrophage bacteriocidal activity was significantly greater (P < or = 0.05) in macrophages from individual immune fish (93.4%) compared to macrophages from individual susceptible fish (85.4%). The kinetics of macrophage killing showed a linear increase in bacteriocidal activity from 1 to 3 h. Opsonization with immune serum enabled macrophages from immune fish to kill bacteria more effectively (93.8 vs. 75.9%) at 2.5 h. Opsonization of E. ictaluri with immune serum significantly suppressed the killing ability of macrophages from susceptible fish (46.2%) at 2.5 h. The results suggest that macrophages from fish immune to ESC had a greater capacity to kill E. ictaluri than macrophages from susceptible fish especially when E. ictaluri were opsonized with anti-E. ictaluri antibody.

Animals↗
PubMed2003

Protective effect of cutaneous antibody produced by channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius multifiliis Fouquet on cohabited non-immune catfish.

Fish which survive a sublethal ichthyophthiriasis acquire protective immunity against Ichthyophthirius multifiliis Fouquet (Ich). This study evaluated the protective effect of cutaneous antibody secreted by channel catfish, Ictalurus punctatus (Rafinesque), immune to Ich on cohabited non-immune catfish. Non-immune and immune fish controls were separately maintained and infected with theronts. The Ich infection was assessed by scoring 0, < 50, 50-100, and > 100 trophonts fish(-1) at 5 days post-infection. The results of infection showed that cohabited fish at the ratio of 15 non-immune to two immune fish had < 50 trophonts fish(-1). Eighty per cent of the cohabited fish at the ratio of 10 non-immune to two immune fish showed 0 or < 50 trophonts fish(-1). The 76% of control non-immune fish had more than 100 trophonts fish(-1). The control immune fish had 0 trophonts fish(-1). Anti-Ich antibody was detected using enzyme-linked immunosorbent assay in water samples taken from tanks containing immune fish after the water samples were concentrated 40-fold. The study suggests that immune fish cohabited with non-immune fish may protect non-immune fish against Ich infection.

Animals↗
PubMed1985

Optimization of conditions for production of channel catfish ovary cells and channel catfish virus DNA.

Medium supplements were examined for their effect on the growth of channel catfish ovary cells. It was found that the usual serum supplement of 10% fetal calf serum could be successfully replaced with a combination of 5% fetal calf serum and a mixture of insulin, transferrin, and selenous acid. It was also found that these cells could be grown in a more efficient manner on microcarrier beads. This type of culture produced 14 times the number of cells per milliliter of total medium used compared with the usual tissue culture flasks used for cell growth. The microcarrier system also provided for greater production efficiency of DNA from channel catfish virus, a virus that infects this cell line.

Journal Article↗
PubMed1976

Seasonal concentrations of dieldrin in water, channel catfish, and catfish-food organisms, Des Moines River, Iowa--1971-73.

Concentrations of dieldrin in aquatic insects, crayfish, minnows, and small carpsuckers, and muscle tissue of channel catfish (Ictalurus punctatus) were compared with the dieldrin content of Des Moines River water in 1971-73. Monthly mean concentrations of dieldrin in river water and most aquatic organisms were highest in June and July, soon after aldrin had been applied to corn land in the watershed. Several groups of aquatic organisms also exhibited high dieldrin levels in the fall when the dieldrin content of river water was seasonally low. The influence of temperature on metabolic rate and enzyme activity and the differences in body fat content were suggested as probable causes of variations observed in the dieldrin content of aquatic organisms.

Agriculture↗
PubMed1989

Activation of complement by different immunoglobulin heavy chain isotypes of the channel catfish (Ictalurus punctatus).

The approximately 750,000 mol. wt tetrameric Ab population from catfish immunized with sheep erythrocytes (E) was purified and shown to sensitize E to the hemolytic activity of catfish serum complement. Catfish complement was heat-labile at 45 degrees C and was hemolytically inactivated by both EGTA and EDTA. Hemolytic activity of EDTA treated serum was restored by the addition of both Ca+ and Mg2+ ions. In comparative assays, the CH50 titers of catfish sera were similar to the CH50 titers of human sera. Catfish complement was hemolytically active at incubation temps ranging from 3 to 40 degrees C, which suggests that complement should be functional throughout the catfish's normal environmental temps. Human complement exhibited considerable cross-reactivity when assayed on catfish Ab sensitized E at 25 degrees C; however, at 3 degrees C, no hemolytic activity was observed. They findings suggest that there may be structural differences between the complement component(s) of these two systems. Mouse mAbs to the recently described H chain isotypes of catfish Ig were used to fractionate catfish anti-sheep E antisera. Each of the purified isotypes contained Ab which sensitized E to the hemolytic activity of catfish complement. Dose-response analysis of the average number of hemolytic sites per cell, as a function of relative Ab concn, predicts that the catfish tetramer conforms to the one-hit theory of immune hemolysis. Purified catfish anti-fluorescein Ab of each isotype was also reacted with fluorescein-labeled sheep E and the level of complement-mediated hemolysis determined. Dose-response analysis indicated that similar numbers of Ab molecules bound to haptenated E resulted in similar levels of complement-mediated lysis. Lastly, the effect of cell surface hapten density of target cells was examined. These studies, similar to those reported with human IgM, showed that the binding of the catfish tetramer to a cell surface hapten may not be sufficient to activate lytic complement.

Animals↗
PubMed2002

Dietary and seasonal effects on the dorsal meat lipid composition of Japanese (Silurus asotus) and Thai catfish (Clarias macrocephalus and hybrid Clarias macrocephalus and Clarias galipinus).

The effects of dietary lipids and seasonal variation on the lipids of wild and cultured catfish (Japanese catfish, Silurus asotus; Thai catfish, Clarias macrocephalus and hybrid Clarias macrocephalus x Clarias galipinus) were determined by analysis of the lipid content and fatty acid composition of their dorsal meat. The predominant fatty acids of dorsal meat were 16:0, 18:1n-9, 18:2n-6, 20:4n-6 (arachidonic acid, AA), and 22:6n-3 (docosahexaenoic acid, DHA). The DHA content in the diet of Japanese catfish was higher than that in the diet of Thai catfish, and this was reflected in the dorsal meat of the Japanese catfish, which had a remarkably high percentage of DHA compared with the meat of the Thai catfish. Cultured Japanese catfish had a higher percentage of 18:2n-6 than Thai fish and a lower percentage of AA in winter than in summer season. There were also seasonal variations in the percentage of n-6 fatty acids in Japanese catfish. In summer, the fatty acid composition of the cultured Japanese catfish was similar to that of the wild catfish. These fatty acid changes in the lipid classes, triacylglycerol, phosphatidylcholine and phosphatidylethanolamine were similar to those observed for total lipids. These results indicate that the percentage of DHA in the dorsal meat of catfish is influenced by dietary fatty acid, and it may be that it can be increased in cultivated fish by administering a diet containing a large amount of DHA.

Animals↗