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At least 37 records · Page 2Linked to original sources
PubMed2000

High mobility of proteins in the mammalian cell nucleus.

The mammalian cell nucleus contains numerous sub-compartments, which have been implicated in essential processes such as transcription and splicing. The mechanisms by which nuclear compartments are formed and maintained are unclear. More fundamentally, it is not known how proteins move within the cell nucleus. We have measured the kinetic properties of proteins in the nucleus of living cells using photobleaching techniques. Here we show that proteins involved in diverse nuclear processes move rapidly throughout the entire nucleus. Protein movement is independent of energy, which indicates that proteins may use a passive mechanism of movement. Proteins rapidly associate and dissociate with nuclear compartments. Using kinetic modelling, we determined residence times and steady-state fluxes of molecules in two main nuclear compartments. These data show that many nuclear proteins roam the cell nucleus in vivo and that nuclear compartments are the reflection of the steady-state association/dissociation of its 'residents' with the nucleoplasmic space. Our observations have conceptual implications for understanding nuclear architecture and how nuclear processes are organized in vivo.

Biological Transport↗
PubMed1987

Effects of denervation induced by 6-hydroxydopamine on cell nucleus activity of arterial and cardiac cells of the dog.

1 Denervation was induced in dogs by 6-hydroxydopamine (6-OHDA) treatment. The effects of this on cell nucleus activity of smooth muscle cells and fibroblasts of the mesenteric artery (main trunk and jejunal branches) and of myocardial cells, fibroblasts and endothelial capillary cells of the heart were studied. 2 Changes in the nucleii or cell size measured with light microscopy morphometric techniques and frequency of nucleoli in myocardial cells were chosen as indices of cell nucleus activity state. 3 Noradrenaline depletion 5 days after initiation of 6-OHDA administration was much more marked in the heart (with a content of 10% of control values) than in the arterial vessels (with a content of 50% of control values). 4 Denervation by 6-OHDA led to an increase in the synthetic activity state of the cell nucleus in all cell types and tissues. 5 Plasma noradrenaline and adrenaline levels were strikingly increased by 6-OHDA treatment. 6 The results strongly support the hypothesis of a nuclear modulating effect of the sympathetic innervation upon the effector cells. The incapacity of high noradrenaline plasma levels to prevent the consequences of sympathetic denervation on the effector cells appears to indicate that in the sympathetic innervation there is a 'trophic factor' which is not identical with noradrenaline.

Animals↗
PubMed1990

Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus.

The yeast cell nucleus has previously been shown to be divided into two regions by a variety of microscopic approaches. We used antibodies specific for the 2,2,7-trimethylguanosine cap structure of small nuclear ribonucleic acids (snRNAs) and for a protein component of small nuclear ribonucleoprotein particles to identify the distribution of small nuclear ribonucleoprotein particles within the yeast cell nucleus. These studies were performed with the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae. By using immunofluorescence microscopy and immunoelectron microscopy, most of the abundant snRNAs were localized to the portion of the nucleus which has heretofore been referred to as the nucleolus. This distribution of snRNAs is different from that found in mammalian cells and suggests that the nucleolar portion of the yeast nucleus contains functional domains in addition to those associated with RNA polymerase I activity.

Antibodies↗
PubMed1975

[Electron microscopy studies on the proliferation of foot-and-mouth disease virus in cell cultures. II. Changes in nucleic acid metabolism of the cell nucleus].

BHK cells were infected with FMD virus and treated with tritium-labelled thymidine and uridine for examination by autoradiography under the electron microscope. Labelling of the DNA, examined by autoradiography under the optical microscope, showed inhibition of 3H-thymidine incorporation. For demonstrating RNA labelling of nuclei, some cells were treated with actinomycin D and others were left untreated. Under the lectron microscope there was no evidence of increased 3H-uridine incorporation in the untreated cells after virus infection, but actinomycin treatment increased RNA labelling in extranucleolar parts of the nucleus, evidently RNA synthesis independent of DNA. There was evidence of some synthesis of virus-specific RNA in the nuclei. The extent of virus-specific RNA synthesis in the cytoplasm was less extensive than in the nucleus.

Animals↗
PubMed2006

The average number of alpha-particle hits to the cell nucleus required to eradicate a tumour cell population.

Alpha-particle emitters are currently being considered for the treatment of micrometastatic disease. Based on in vitro studies, it has been speculated that only a few alpha-particle hits to the cell nucleus are considered lethal. However, such estimates do not consider the stochastic variations in the number of alpha-particle hits, energy deposited, or in the cell survival process itself. Using a tumour control probability (TCP) model for alpha-particle emitters, we derive an estimate of the average number of hits to the cell nucleus required to provide a high probability of eradicating a tumour cell population. In simulation studies, our results demonstrate that the average number of hits required to achieve a 90% TCP for 10(4) clonogenic cells ranges from 18 to 108. Those cells that have large cell nuclei, high radiosensitivities and alpha-particle emissions occurring primarily in the nuclei tended to require more hits. As the clinical implementation of alpha-particle emitters is considered, this type of analysis may be useful in interpreting clinical results and in designing treatment strategies to achieve a favourable therapeutic outcome.

Alpha Particles↗
PubMed2002

CNN-Gd(3+) enables cell nucleus molecular imaging of prostate cancer cells: the last 600 nm.

Molecular imaging is defined as the characterization and measurement of biological processes at the cellular and molecular level. Molecular imaging, therefore, necessitates a sufficient amount of contrast agent within the cell. Consequently, we realized that the intracellular uptake and cell compartment specificity of the commonly used interstitial contrast agent gadolinium (Gd(3+)) with a cell-nucleus directed peptide module could be helpful. This modular molecule is characterized by a Gd(3+)-complex module that is bound to a transmembrane transport unit (TPU) of human origin and further to a nucleus-directed address module (nuclear localization sequence) resulting in a specific cell nucleus-directed nuclear localization sequence-conjugated Gd(3+)-complex (CNN-Gd(3+)-complex). By use of magnetic resonance imaging, Gd(3+) was detected within DU-145 prostate cancer cells after only 10 min. The nuclear localization was confirmed with confocal laser scanning microscopy. The resulting MRI signal enhancement only slightly decreased over the next 48 h compared with an absolute loss of signal enhancement after only 8 h when a random target sequence was used. Therefore, our method seems promising for in vivo application in molecular imaging.

Amino Acid Sequence↗