Lymphocytes as models for the study of mammalian cellular differentiation.
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Animals↗
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Results for “Cellular differentiation”
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Isozyme patterns of branched-chain amino acid transaminase during cellular differentiation and carcinogenesis.
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Animals↗
Genetics of cellular differentiation: stable nuclear differentiation in eucaryotic unicells.
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Animals↗
Lactate dehydrogenase isoenzyme pattern as a measure of cellular differentiation in lymphocytic cells.
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B-Lymphocytes↗
[Cellular differentiation and malignant transformation (author's transl)].
The mechanisms and determination of differentiation of normal and cancer cells were compared by distinguishing them from the phenomena of regulation, retrodifferentiation and maturation. Reference is made to the laws of order in time and space which are superimposed on the common genetic code during the various types of differentiation. In conclusion, the particular mechanisms of malignant differentiation are discussed by taking melanoma and tumor formation by papova-viruses as examples.
Animals↗
3D STED Imaging of Isolated Arabidopsis thaliana Nuclei.
Microscopy imaging of chromatin offers valuable insights into its spatial organization in the nucleus, a novel epigenetic dimension influencing the genome's functions. Particularly, visualization at the nanoscale in single cells is uniquely complementary to molecular profiling methods averaging chromatin configuration and composition over thousands of cells. How are chromatin and chromosomal domains distributed in relation to gene expression? How variable are these configurations? How do chromatin domains evolve in structure, composition, and distribution during cellular differentiation or cellular responses to environmental stimuli? Super-resolution microscopy techniques, like stimulated emission depletion (STED), are key in answering such questions. However, such imaging techniques are not often used in the field of plant cell biology compared to mammalian counterparts, which has greatly advanced our understanding of the 3D principles in genome organization. In an effort to bridge this gap, we provide a clear guide for isolating, embedding, immunostaining, and STED imaging intact leaf nuclei from Arabidopsis thaliana in 3D.
Arabidopsis↗
Acute myelomonocytic leukemia in children. Possible use of the soft agar culture technique in the differentiation of cellular subtypes.
In 8 children with acute myelomonocytic leukemia (AMML), colony formation in soft agar cultures derived from bone marrow cells was studied in an attempt to differentiate the monocytic (Schilling) from the myelomonocytic (Naegeli) types. The children did not differ markedly in their clinical and morphological parameters. Three in vitro growth patterns were observed: markedly decreased or no growth in 4 cases, extensive growth of granulocytic colonies in 2 cases, and extensive growth of macrophage colonies in the remaining 2. It is suggested that the marrows presenting diminished or no growth patterns are presumably of acute myelogenous leukemia patients with a monocytic component. The excessive granulocytic or macrophage colony growth may be an in vitro indication for an in vivo proliferation of either granulocytic or monocytic leukemic cell lines, and therefore may represent the Naegeli or Schilling variants of AMML respectively. If these observations can be approved in a larger series of AMML patients, this approach can be valuable as another tool in the differential diagnosis of the subtypes of AMML in children.
Adolescent↗
New aspects of preleukemic disorders.
Preleukemic disorders are a controversial group of panmyelopathic disturbances that often precede the emergence of acute myeloblastic or myelomonocytic leukemia. In most instances, these preleukemic disorders are characterized by slowly developing myeloblastosis of the bone marrow. They include preleukemia, primary acquired panmyelopathy with myeloblastosis or smouldering acute leukemia, erythroleukemia, and subacute myelomonocytic leukemia. Sometimes, transitions between these various preleukemic disorders may be observed in a single individual. Abnormalities in cellular differentiation are expressed in cytochemical aberrations and in elaboration of colony forming units by marrow cells of patients with preleukemic disorders. Cytogenic and cellular kinetic abnormalities link preleukemic disorders closely to acute myeloblastic or myelomonocytic leukemia, although in many patients with preleukemic disorders, conversion to acute leukemia is not observed or perhaps not recognized. Understanding pathogenetic and pathophysiological aspects of preleukemic disorders may shed light on aspects of cellular proliferation and cellular differentiation in the acute leukemias.
Acute Disease↗
Diagnosis of bronchogenic carcinoma through the cytologic examination of sputum, with special reference to tumor typing.
The results obtained in the cytologic study of sputa from 630 patients are presented. There were 251 cases of bronchogenic carcinoma; diagnosis through sputum examination was possible in 57.4 per cent of the patients. Abnormal cells were detected in an additional 24.3 per cent. Sputum examination has proven to be a valuable complement by establishing the correct diagnosis when other methods failed. Cancer cells were unequivocally identified in 45.8 per cent of the cases with normal bronchoscopic examination and in 52.4 per cent of the cases in which bronchial biopsy did not include malignant tissue. The same proportion of cases with the various tumor types was obtained by cytologic and by histologic study. However, one of the methods often showed a higher degree of cellular differentiation than the other. The number of cases with undifferentiated cancer or unclassified tumors was markedly reduced when the information concerning cell differentiation available through both methods was used. In this manner, excluding the oat cell carcinomas, only 7.6 per cent of the cases of bronchogenic carcinoma did not show any cellular differentiation. The authors recommend wider use of the information provided by simultaneous evaluation of both cytologic smears and tissue sections in order to achieve a more accurate appraisal of tumor type.
Adenocarcinoma↗
"The diencephalon of Ptilocercus lowii (pen-tailed tree-shrew)".
The pen-tailed tree-shrew (Ptilocercus lowii) has been regarded by Le Gros Clark (1926), and Martin (1968) to possess more primitive morphological and to a certain extent, neuro-anatomical characteristics than the other tree-shrew species belonging to the sub-family Tupaiinae. Investigations have been carried out on the diencephalon of Ptilocercus lowii which has not been completely done by Le Gros Clark and other workers since 1926, to find out whether the diencephalon has actually undegone any phylogenetic changes that should be more advanced than those of the Insectivora or remain more primitive than that of the Tupaiinae. The diencephalic structure that show the most significant phylogenetic features which differe Ptilocerecus from Tupaia are observed in the anterior, dorsolateral and ventrolateral thalamic groups, and the geniculate bodies. The nucleus anteroventralis is, by comparison, much smaller and poorly differentiated from the nucleus anteromedialis. The nucleus anterodorsalis is also comparably large and does not reach the dorsal surface of the thalamus as it does in the primates. The midline nuclei do not show any significant differences, except the notably larger size of nucleus parataenialis in Ptilocercus. The nucleus mediodoralis is small and indifferentiated although it does show some incipient signs of cellular differentiation. The nucleus centralis lateralis is well developed; its inferior (ventral) part is larger than its superior part which is the better developed of the two parts in the tupaiids and primates. The nucleus centrum medianum appears to be very small and forms only a lateral extension of the nucleus parafascicularis. The ventrolateral thalamic group basically consists of nuclei ventrales anterior, lateralis and posterior without intermediate or transitional zones as found in Tupaia and Primates. The nucleus pretectalis is the largest and the best developed of all the elements of the posterior thalamic nuclear group. The lateral geniculate nucleus is not differentiated into laminae like that in Tupaia and Primates; it is merely a homogeneous structure that appears to be smaller in size that the pregeniculate and medial geniculate nuclei. The medial geniculate nucleus is large in proportional size of the whole thalamus and appears to be well differentiated cellularly into several small parts. No remarkable changes are noted in the epithalamus, subthalamus and hypothalamus. However, the nucleus ventromedialis appears to be the most outstanding structure in the infundibular region of the hypothalamus. The mammillary region is of a simpler construction in Ptilocercus than in Tupaia; it does not protrude from the ventral surface of the hypothalamus. In the light of these findings, the phyletic status of Ptilocercus in the family Tupaiidae is discussed.
Animals↗
Cellular origin, differentiation and classification of leukemia and lymphoma cells as based on surface marker analysis.
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Antigens, Surface↗
Cell differentiation during fruiting body formation in polysphondylium pallidum.
The spatial pattern of cellular differentiation was studied during fruiting body formation in Polysphondylium pallidum using 3 different staining methods: Calcofluor fluorescence (cellulose accumulation), neutral red (prestalk cells) and immunofluorescence (prespore cells). Neutral-red staining revealed the existence of a clear prestalk region which becomes evident during aggregation and continues throughout culmination. Immunofluorescent staining demonstrated that cells in the prestalk region gradually lose their presporeness (fluorescence) as they are transformed into differentiated stalk cells. Calcofluor staining revealed that stalk cell differentiation begins during the mid-aggregation phase and that the mode of formation of the main stalk and the side branches differs slightly in morphology. Calcofluor staining also demonstrated the development, during aggregation, of a thick cellulosic girdle with lateral tubular extensions which surround the aggregation streams. The above results are discussed in terms of our present knowledge about differentiation and morphogenesis in cellular slime moulds.
Dictyostelium↗
Integrator promotes the association of TFIID and RNA polymerase II to maintain pluripotency during development.
The mechanisms by which the expression of pluripotency and Polycomb networks are harmonized to allow the transition from pluripotency to a differentiated state have not been fully elucidated. Integrator complex regulates transcription pause release and RNA processing in metazoans. We show that Integrator is required for stemness and plays a critical role as early as day 2 in embryonic development. While the catalytic endonuclease activity enhances cellular reprogramming, Integrator recruits RNA polymerase II (RNAPII) to promoters and super enhancers of pluripotency and Polycomb genes. Integrator coordinates expression of pluripotency and Polycomb networks by fostering the association of RNAPII and basal transcription factors. We pinpoint a critical role for TATA-binding protein-associated factors (TAFs) in Integrator entry into the preinitiation complex. Taken together, beyond its role in RNAPII pause release, Integrator recruitment of RNAPII ensures an orderly cellular differentiation during development.
RNA Polymerase II↗
The cellular aging of rat fibroblasts in vitro is a differentiation process.
Cellular aging of diploid rat fibroblasts in vitro occurs as the result of a three-stage differentiation sequence, taking place in the morphologically recognizable differentiating cell compartment of the fibroblast stem cell system. Simultaneously with an age-dependent morphological differentiation there is an age-dependent biochemical differentiation of the fibroblasts. In cell strains with the genetic background Lewis, the cellular differentiation state, the major histocompatibility complex composition (H-1) and the H-1 complex-controlled expression of endogenous type C viruses determine the ultimate fate of the senescent cell, leading either to cellular degeneration or to cellular neoplastic transformation.
Animals↗
Dynamics of skeletal pattern formation in developing chick limb.
During development of the embryonic chick limb the skeletal pattern is laid out as cartilaginous primordia, which emerge in a proximodistal sequence over a period of 4 days. The differentiation of cartilage is preceded by changes in cellular contacts at specific locations in the precartilage mesenchyme. Under realistic assumptions, the biosynthesis and diffusion through the extracellular matrix of a cell surface protein, such as fibronectin, will lead to spatial patterns of this molecule that could be the basis of the emergent primordia. As cellular differentiation proceeds, the size of the mesenchymal diffusion chamber is reduced in descrete steps, leading to sequential reorganizations of the morphogen pattern. The successive patterns correspond to observed rows of skeletal elements, whose emergence, in theory and in practice, depends on the maintenance of a unique boundary condition at the limb bud apex.
Animals↗
[Critical study of the morphogenesis of trunco-conal malformations].
Anatomical and angiocardiographic studies have made possible discussion of hypotheses of the development and differentiation of the conus and of the trunco-conal orientation of the septum. Three autopsy specimens and two angiocardiogrammes of intertwined great vessels with transposition and partial distortion of the great vessels were studied. The specimen of intertwined great vessels comprised a muscular sub-aortic infundibulum posterior to and greater than the pulmonary infundibulum. In the case of transposition the opposite situation was encountered with a muscular pulmonary infundibulum posterior to and greater than the sub-aortic infundibulum; the plane of the aortic valves was higher than that of the pulmonary valves. The specimen of partial distortion of the great vessels comprised a muscular sub-aortic infundibulum posterior to and greater than the pulmonary infundibulum with the aortic valve set higher and in fibrous continuity with the mitral valve. It is concluded that the relations of the great vessels between each other and with the ventricles depend on the orientation of the trunco-conal septum and on the process of incorporation of the cone. The presence or absence of a posterior muscular infundibulum is not related to the growth or differential reabsorption of the cone but to a process of cellular differentiation. There was no relation between the level of the valves and their spatial orientation and the length of the infundibulum. The continuity or discontinuity between the aortic and atrio-ventricular valves is not determined by the level of the aortic valves.
Female↗
The cytological differentiating potential of pineal parenchymal neoplasms (true pinealomas). A clinicopathological study of 28 tumours.
A series of 28 pineal parenchymal tumours is described, with special reference to the potential of some of these neoplasms to differentiate along glial or ganglionic lines, or both. The more undifferentiated tumours (pineoblastomas, 11 cases) were the most frequent: they are histologically similar to medulloblastomas. One example showed focal differentiation to retinoblastoma at the primary site. The histological features of pineoblastomas merged with those of pineocytomas (7 cases), in which the lobular architecture is reminiscent of that of the mature pineal gland. In addition, 10 further examples in the group of pineocytomas showed more advanced differentiation as follows: towards astrocytes only (2 cases), towards ganglion cells only (1) case) and towards both astrocytes and ganglion cells (gangliogliomas) (7 cases). Confirmation of the pineal parenchymal nature of these neoplasms and of their differentiating potential was provided by a modification of the Achúcarro-Hortega's silver carbonate impregnation technique for pineal parenchymal cells, by specific silver impregnations for axonal processes, and by an immunoperoxidase stain for glial fibrillary acidic (GFA) protein. Electron microscopy of one new example of pineocytoma with neuronal and astrocytic differentiation demonstrated the presence of numerous microtubules, of clear-centred and dense-core vesicles, and of synaptic complexes. Seven illustrative clinical histories with pathological findings are presented. The identification of special features of cellular differentiation is of importance in evaluating the biological behaviour of these neoplasms since a definite correlation can be established between the patient's age, some of the cytological variants, and the malignant potential of the tumour. Pineoblastomas are highly malignant neoplasms of children and young adults which disseminate widely throughout the cerebrospinal fluid pathways. -ineocytomas without cellular evidence of further differentiation occur at any age and are also clinically malignant, but with a somewhat lesser tendency to metastasize than pineoblastomas. Pineobytomas with astrocytic differentiation occur in adults and may be either slowly growing or malignant. Pineocytomas with neuronal or with neuronal and astrocytic differentiation occur in later life, remain localized, and are relatively benign. Since the latter account for approximately one-third of pineal parenchymal tumours and are likely to be relatively radio-resistant, tissue diagnosis is imperative for a determination of the therapeutic approach. Radiation to the entire neuraxis should be administered to patients with pineoblastomas and malignant pineocytomas in view of their high frequency of cerebrospinal metastasis. An accurate histological classification of these tumours therefore carries important clinical and therapeutic implications...
Adolescent↗
[Correlations between the processes of cell differentiation and development of blood vessels in the (chick embryo) adenohypohhysis].
Utilizing semi-thin and, respectively, thick sections after indian ink injections, we have analyzed morphohistogenesis and vessel development in the adenohypophysis of chick embryos from the 4th to the 19th incubation day. Our preliminary results indicate that a close correlation exists between cellular differentiation and vasculogenesis. Vessels begin to enter the gland only at the 6th day when the first endocellular granules become detectable, and, from the 10th day, they markedly increase in number and size, gradually acquiring a peculiar sinusoidal arrangement around cellular groups, as cell differentiation further progresses.
Animals↗