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Double-exposure duplication of preoperative and postoperative 2 X 2 transparencies.

Before and after photographs in plastic surgery are an essential part of the patient's record. They also provide visual data to supplement both primary and continuing education lectures. When successive photographs are used in lectures, a sharp visual memory is required to compare effectively the preoperative and postoperative photograph. The method presented in this article uses a specially constructed frame used as an accessory for one of the common commercial slide copiers. This device makes it possible to copy, duplicate, and frame preoperative and postoperative 2 X 2 transparencies onto a single 2 X 2 transparency without any additional work on the original, such as framing, cutting, and remounting. This technique leaves the original preoperative and postoperative 2 X 2 transparency slides intact for possible use elsewhere in the same lecture or subsequent lectures on the same subject.

Audiovisual Aids

Acute-onset sarcoidosis presenting as workplace-related hyperreactive airway disease.

Bechtel et al [Am Rev Respir Dis 124:759-761, 1981] have reported a high prevalence of positive methacholine challenge tests in patients with sarcoidosis. We report a patient with sarcoidosis who was identified because of symptoms resulting from exposure to workplace irritants. The presence of hyperreactive airways was confirmed by a positive methacholine challenge.

Adult

Demonstration of digital radiographs by means of ink jet-printed paper copies: pilot study.

Different digital medical images have been printed on paper with a continuous ink jet printer, and the quality has been evaluated. The emphasis has been on digital chest radiographs from a computed radiography system. The ink jet printing technique is described as well as the handling of the image data from image source to printer. Different versions of paper prints and viewing conditions were compared to find the optimum alternative. The evaluation has been performed to maximize the quality of the paper images to make them conform with the corresponding film prints and monitor images as much as possible. The continuous ink jet technique offers high-quality prints on paper at a considerably lower cost per copy compared with the cost of a film print. With a future switch-over from diagnosing of digital images on film to diagnosing them on monitors, hard copies for demonstration purposes will occasionally be needed. This need can be filled by ink jet-printed paper copies.

Copying Processes

Photoduplication and fluorescence measurement in polyacrylamide gel electrophoresis.

A simple method of photoduplication of gel electrophoresis, visualized with fluorescent reagents, is described. The procedure is convenient and rapid and requires no camera or expensive equipment. Using electrophoresis duplicating paper (Kodak), positive prints suitable for documentation or publication may be obtained. With usual photographic paper, negative prints may be obtained, allowing reliable measurement by scanning. The technique may be applied to protein or nucleic acid electrophoresis.

Copying Processes

A xerographic method for the quantitative assessment of atherosclerotic lesions.

A new method has been developed for the quantitative assessment of atherosclerotic lesions, in which a conventional xerox machine is the sole equipment used. As an example, the aorta from a cholesterol-fed rabbit was removed in toto, opened longitudinally along its anterior margin, flattened in a transparent polypropylene bag and then xeroxed. The outlines of the atherosclerotic lesions were traced on translucent graph paper ruled into 1 mm squares. The squares within those outlines were counted and expressed as a percentage of the whole surface. This xerographic method is rapid and reproducible and is achieved without changing the magnification; it gives comparable or better results than other staining methods. As the aortic specimen is undamaged by fixation or staining, the specimen can be used for subsequent morphological or biochemical studies. Thus, the degree of surface involvement can be correlated with other morphological or biochemical changes in the same arterial samples.

Animals

A study of the quality of duplicated radiographs.

The resolution, contrast, and clinical appearance of radiographs and duplicate radiographs made with two types of duplicating film were compared. Duplicating conditions evaluated were type and shape of light, light-film distance, type of exposure surface, and developer temperature. Major observations were as follows: both Kodak and DuPont films produced clinically acceptable duplicates; Kodak film was faster; DuPont film responded better in incandescent photoflood light than Kodak film; clear glass with appropriate light-film distance was the best exposure surface.

Absorptiometry, Photon

Sensitometric comparison of unexpired and expired duplicating films used in dentistry.

This investigation compared the sensitometric properties of unexpired and expired duplicating films. The characteristic curve derived for a duplicating film is the negative slope of a solarization curve; that is, it is different in direction to that of a regular x-ray film. An expired duplicating film is slower than an unexpired duplicating film. Unlike a negative working film, which gains fog past its expiration date, a positive working film (for example, a duplicating film) gains contrast past its expiration date. An expired duplicating film has a narrower latitude than that of an unexpired duplicating film for a useful density range. Clinically, a duplicating film can be used years after its expiration date even though there is a change in its speed and contrast.

Copying Processes

The automation of routine calculations and graphing for alkaline filter elution analysis on a computer-controlled liquid scintillation analyzer.

The data calculations and graphing functions for the widely used alkaline filter elution technique have been completely automated. This saves considerable time and increases both efficiency and accuracy by eliminating human error. The automation was accomplished utilizing Lotus 1-2-3 and Lotus Graphwriter II on a Packard Tri-Carb 1900CA liquid scintillation analyzer containing a built-in pico-XTE computer. A batch file is used to control the overall execution of the process. It copies the data stored by the 1900CA into the Lotus subdirectory and invokes Lotus 1-2-3. A Lotus macro automatically imports the data file, performs the calculations, and prints the results. Graphwriter II is then invoked by the batch file and the charts are composed and graphed. Finally, the instrument operating software for the 1900CA is reentered and sample analysis can be resumed for any unanalyzed samples.

Alkalies

The RecE recombination pathway mediates recombination between partially homologous DNA sequences: structural analysis of recombination products.

Escherichia coli generalized recombination, utilizing the RecA RecB recombination pathway, requires large stretches (70-200 bp) of complete DNA sequence homology. In contrast, we have found that the RecE pathway can promote recombination between DNA with only short stretches of homology. A plasmid containing 10 partially homologous direct repeats was linearized by digestion with specific restriction enzymes. After transformation, a RecE+ (sbcA) host was able to circularize the plasmid by recombination between partially homologous direct repeat sequences. Recombination occurred in regions of as little as 6 bp of perfect homology. Recombination was enhanced in the regions adjacent to restriction sites used to linearize the plasmid, consistent with a role of double-strand breaks in promoting recombination. A mechanism is proposed in which the 5' exonuclease, ExoVIII, produces 3' single-stranded ends from the linearized plasmid. These pair with other sequences of partial homology. Partial homologies in the sequences flanking the actual join serve to stabilize this recombination intermediate. Recombination is completed by a process of "copy and join." This recombination mechanism requires less homology to stabilize intermediates than the degree of homology needed for mechanisms involving strand invasion. Its role in nature may be to increase genomic diversity, for example, by enhancing recombination between bacteriophages and regions of the bacterial chromosome.

Base Sequence

Xerox copy of the dental cast for analysis of the stability of mandibular osteotomy.

Stability of the mandible was analysed with a Xerox copy of the dental cast in 35 cases of mandibular prognathism corrected by ramus osteotomies. The occlusal relationship in the Xerox copy was illustrated by superimposing two contact points on the upper and lower dental arches and the change in the position of the lower dental arch in relation to the upper dental arch was obtained by superimposing the latter on preoperative, predicted postoperative, and six months postoperative occlusions. The stability of the mandible was analysed by measuring the movement of five landmarks (two posterior, two molar and one incisor points) set around the lower dental arch to represent the movement of the mandible. The relapse was estimated by the movements of the landmarks from the predicted postoperative occlusion to the six months postoperative occlusion. The mean estimated anterior relapses at the posterior and molar points with the larger predicted movement and the incisor point were between 0.9 mm. and 2.0 mm. at six months postoperatively, whereas on the side with the smaller predicted movement, the points moved posteriorly by 0.6 mm. and 0.5 mm. Despite the fact that the amount of the predicted lateral movement was much smaller than that of the predicted posterior movement at operation, the posterior points were estimated to relapse laterally by 2.0 mm. and 1.7 mm.(ABSTRACT TRUNCATED AT 250 WORDS)

Cephalometry