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[Jellinek's developmental stages and their reality under our sociocultural conditions].

In a group of 250 hospitalized male patients with the diagnosis 303 the author tested the aptness of Jellinek's developmental stages of alcoholism which are widely used in Czechoslovak alcohology. It was demonstrated that the developmental stages are not integrated developmental stages, that they lack the necessary compactness in the chronological sequence and in the frequency of individual phenomena. Loss of control (8) in the reported form is closer to the onset of development and the frequency of palimpsests (7) develops later, usually is overlaps with prolonged drunkedness (31). At the onset of development dominates "non-adaptive" drinking evaluated frequently as loss of control and in the more advanced stage the constant incidence of palimpsests causes frequent intoxication and declining tolerance. Among individual phenomena the following are important: in stage I regular contact with the drug (04) and increased tolerance (05), in stage II secret drinking (2) and frequency of palimpsests (7), in stage III signs derived mostly from rationalization and alcohol-centered behaviour and finally in stage IV impaired thinking (33), reduced tolerance (37) and possible ethic degradation (32). As to different developmental stages the chronic stage sounds most convincing.

Adult↗

N-Glycans of Caenorhabditis elegans are specific to developmental stages.

We have examined the N-glycans present during the developmental stages of Caenorhabditis elegans using two approaches, 1) a combination of permethylation followed by MALDI-TOF mass spectrometry (MS) and 2) derivatization with 2-aminobenzamide followed by separation by high-performance liquid chromatography and analyses by MALDI-TOF MS, post source decay (PSD) MS, and MALDI-QoTOF MS/MS. The N-glycan profile of each developmental stage (Larva 1, Larva 2, Larva 3, Larva 4, and Dauer and adult) appears to be unique. The pattern of complex N-glycans was stage-specific with the general trend of number and abundance of glycans being Dauer approximately = L1 > adult approximately = L4 > L3 approximately = L2. Dauer larvae contained complex N-glycans with higher molecular masses than those seen in other stages. MALDI-QoTOF MS/MS of Hex4HexNAc4 showed an N-acetyllac-tosamine substitution not previously observed in C. elegans. Phosphorylcholine (Pc)-substituted glycans were also found to be stage-specific. Higher molecular weight Pc-containing glycans, including fucose-containing ones such as difucosyl Pc-glycan (Pc1dHex2Hex5HexNAc6) seen in Dauer larvae, have not been observed in any organism. Pc2Hex4HexNAc3, from Dauer larvae, when subjected to PSD MS analyses, showed Pc may substitute both core and terminally linked GlcNAc; no such structure has previously been reported in any organism. C. elegans-specific fucosyl and native methylated glycans were found in all developmental stages. Taken together, the above results demonstrate that in-depth investigation of the role of the above N-glycans during C. elegans development should lead to a better understanding of their significance and the ways that they may govern interactions, both within the organism during development and between the mobile nematode and its pathogens.

Animals↗

Modulatory subdomains of the HS2 enhancer differentially regulate enhancer activity in erythroid cells at different developmental stages.

The HS2 enhancer in the locus control region of human beta-like globin genes displays developmental-stage-independent enhancer function. The mechanism by which it regulates the transcription of the globin genes in erythroid cells throughout development is not fully understood. In this paper we dissect the HS2 enhancer into an enhancer core and five modulatory subdomains M1 to M5. The enhancer core possesses developmental-stage-independent enhancer activity. The modulatory subdomains by themselves do not possess such enhancer activity, but they apparently respond to environmental signals and modulate enhancer core activity in a developmental-stage specific manner. M1 located 5' of the core strongly stimulates core activity in K562 cells at the embryonic stage. M2 and M3 located 3' of the core strongly stimulate core activity in MEL cells at the adult stage. Moreover, M3 suppresses core activity at the embryonic stage and exhibits an adult-stage-selector activity. These findings indicate that the apparent developmental-stage-independence of the HS2 enhancer is a result of multiple interactions between the core and the modulatory subdomains located both near and far from the core.

Adult↗

[Antigen analysis of Angiostrongylus cantonensis in different developmental stages].

OBJECTIVE: To analyze the difference among antigens of Angiostrongylus cantonensis in different developmental stages and identify dominant diagnostic antigen for angiostrongyliasis. METHODS: Antigens of A. cantonensis in different developmental stages were analyzed by SDS-PAGE and immunoblot. RESULTS: The protein bands of all developmental stages were similar on SDS-PAGE. The Mr 40000, 50000, 66000 and 80000 antigens reacted not only with the sera of rats infected by A. cantonensis but also with the sera of normal rats. The Mr 104000 antigen could be discerned by sera of rats infected with A. cantonensis for 2 weeks. The Mr 32000 antigen could be recognized by sera of rats 2 weeks after infection, and the reaction became stronger with the infection continued. CONCLUSION: The Mr 40000, 50000, 66000 and 80000 antigens might result in the unspecific reaction in the immunodiagnosis of angiostrongyliasis using the crude antigen of A. cantonensis. The Mr 104000 of larva, Mr 33000 of adult females and Mr 32000 of the worms might be used as candidate antigens in early diagnosis and epidemiological survey of angiostrongyliasis.

Angiostrongylus cantonensis↗

Integrative articular cartilage repair: dependence on developmental stage and collagen metabolism.

OBJECTIVES: The objectives of this research were to determine whether the integrative repair of bovine cartilage explants was dependent on developmental stage, and whether observed differences in integration with developmental stage were related to deposition of newly synthesized collagen and lysyl oxidase-mediated collagen cross-linking. METHODS: Pairs of fetal, newborn calf, and adult bovine cartilage blocks were cultured in partial apposition for 2 weeks in medium supplemented with serum, ascorbate, and [3H]proline. Following culture, mechanical integration between apposed cartilage blocks was assessed by measuring adhesive strength in a single-lap shear configuration. Formation and stabilization of newly synthesized protein and collagen was investigated by determination of [3H]proline and [3H]hydroxyproline in tissue digests and guanidine extracts. RESULTS: Calf cartilage exhibited a relatively high integrative repair phenotype, achieving an adhesive strength that was three--four-fold that of adult or fetal specimens. The low and high integrative repair phenotypes appeared related in part to different levels of collagen biosynthesis, which was approximately four--five-fold higher in calf cartilage samples than in the adult. However, fetal cartilage also exhibited a high level of biosynthesis. The different integrative repair phenotypes were not associated with marked differences in the kinetics of chemical stabilization of newly synthesized collagen, as the proportion of incorporated [3H]proline and newly-formed [3H]hydroxyproline that was resistant to extraction by 4M guanidine-HCl following culture was similar for cartilage from all developmental stages. Integration of calf cartilage appeared to depend on lysyl oxidase-mediated collagen cross-link formation, since inclusion of beta-aminopropionitrile (BAPN) in the culture medium completely eliminated development of adhesive strength. BAPN treatment also increased the percentage of newly synthesized protein in the guanidine extracts from 10% to 36% of the total, and that of newly synthesized collagen from 2% to 20%, while having only slight inhibitory effects on overall protein and collagen biosynthesis. CONCLUSION: The finding that cartilage exhibits enhanced integrative repair at a certain developmental stage suggests that it may ultimately be possible to enhance repair when needed in clinical situations.

Animals↗

Differential expression of the fibroin gene in developmental stages of silkworm, Antheraea mylitta (Saturniidae).

Fibroin gene expression during the larval developmental stages of the Saturniid silkworm, Antheraea mylitta, was analyzed. Northern blot analysis of larval silk gland total RNA using the fibroin gene as a probe showed that fibroin is expressed in the intermoult stages and repressed during the moulting stages. Abundance of fibroin transcripts gradually increased from the third to fifth intermoult stage, reaching a peak in the fifth intermoult. Transcripts declined during the early spinning stage. Western blot analysis of fibroin protein production with anti-fibroin antibody confirmed the differential fibroin expression, in accordance with fibroin mRNA synthesis. Dot blot hybridization of genomic DNA isolated from each larval developmental stage with the labelled fibroin gene showed that at the genomic level, the relative concentration of the fibroin gene was constant throughout the developmental stages. Our data confirm that fibroin gene expression in A. mylitta, like in B. mori, is transcriptionally controlled and shows differential temporal variations.

Animals↗

Developmental stages of occupational therapy and the feminist movement.

Developmental stages characterize the growth of both the feminist movement and the field of occupational therapy. The human development stages described by Erik Erikson are used to compare the growth stages of these two groups to the human sequence. It is hypothesized that both female-dominated groups began with an "infancy" period in the early 1900s characterized by the beginnings of identity and confidence in their respective groups. Both continued to develop through stages corresponding to the childhood stages characterized by Erikson and into the adolescent acting out stages as characterized by the demonstrations of the 1960s. Present and future trends are explored in relation to developmental stages, as well as to implications for the field of occupational therapy in education, theory, and practice.

Adult↗

[Ultrastructural antigenic localization in Paragonimus iloktsuenensis during developmental stage by immunogold labeling method].

Antigenic localization in Paragonimus iloktsuenensis worm tissues (tegument, intestine and vitelline gland) in different developmental stages of 2 weeks, 3 weeks, 4 weeks, 5 weeks and 33 weeks from albino rats (Sprague-Dawley) infected with P. iloktsuenensis was observed by electron microscopy. These worm tissues of different developmental stage of P. iloktsuenensis was observed on electromicrograph by immunogold labeling method using P. iloktsuenensis infected rat serum of 10 weeks. Antigenic localization was demonstrated as labeling of gold particles in tissues on electronmicrograph. In tegument, gold particles were labeled on tegumental tissue, generally more numerous on secretory granules in tegumental syncytium 2 weeks than those on the other elder developmental stages, but there was a little variation in antigenicity according to individual worm tissue. In general, antigenicity in tegumental tissue was not strong (gold particles: 0.1-5/1 microns 2). In intestine, a large number of gold particles (15-18/1 microns 2) were labeled in intestinal epithelium. Gold particles were concentrated especially on secretory granules in cytoplasm, and gold particles were labeled not only in cytoplasmic protrusions, but also in intestinal luminal contents. Intensity of labeling of gold particles was not correlated with developmental stage of worms. In vitelline gland, a large number of gold particles were labeled on vitelline globules. The gold particles in vitelline globules (8-11/1 microns 2) were concentrated in protoplasm among segmental globules.

Animals↗

Fecundity, fertility, and developmental stage survivorship of Eretmapodites quinquevittatus.

This laboratory study analyzed fecundity, fertility (percentage hatched), and developmental stage survivorship of Eretmapodites quinquevittatus. Fecundity for females averaged 190.4 eggs, whereas paired (one male/female pair) females averaged 100.5 eggs. Fertility for autogenous eggs from paired females was 84.4%, which was significantly greater than percentage hatched of anautogenous eggs (67.3%). Percentage hatched of autogenous eggs from experimental groups (25 males/25 females) was 87.8%, whereas percentage hatched of anautogenous eggs was 74.5%. Developmental stage survivorship was significantly different for every stage of development of progeny from autogenous eggs, except the egg stage, when comparing experimental groups with pairs. Developmental stage survivorship of progeny from anautogenous eggs was significantly different only at the egg stage and 1st instar when comparing experimental groups and pairs.

Animals↗

The cuticular biology in developmental stages of Ascaris suum.

The cuticles from distinct developmental stages of Ascaris suum were isolated by a combination of mechanical disruption and detergent treatment of larvae or by surgical removal of cuticle from adults. Proteins from the isolated cuticles were solubilized with SDS and 2-mercaptoethanol (2ME) and analyzed by PAGE. Cuticular proteins from the third and fourth larval stages (L3 and L4) were comparable to adult, but differences in the number of bands were observed. The soluble proteins from the adult, L3 and L4 were readily degraded by bacterial collagenase, suggesting that these proteins are collagen-like structural elements of the cuticle. The soluble proteins from the L2 differed from the adult and other larval stages in both the number and molecular weight of protein bands and their lack of collagenase sensitivity. Antibodies made against the soluble cuticular proteins reacted with the medial and basal layers of the cuticle but not the external cortical or epicuticular regions. A significant amount of the cuticle was not solubilized by 2ME and was not digested by bacterial collagenase. These insoluble cuticular proteins were probably derived from the epicuticular and external cortical regions of the cuticle. Different developmental stages of A. suum were biotinylated and examined by electron microscopy. An organic soluble biotin reagent labeled all stages in a transcuticular pattern, while an aqueous soluble biotin labeled only the external cortical and epicuticular regions of the L4 and adult cuticle. These data indicate the presence of a hydrophobic barrier in the cuticle of later stages of the parasite.

Animals↗

Direct immunofluorescent staining of Leucocytozoon caulleryi of different developmental stages.

Direct immunofluorescence was first used successfully for the staining of Leucocytozoon caulleryi of different developmental stages, such as the 2nd generation schizont and merozoite, gametocyte, zygote, ookinete, and sporozoite. Fluorescent antibody of IgG nature prepared from sera of chickens over a period from the 21st to the 100th day after sporozoite inoculation was used in this experiment. The cross reactivity on different developmental stages of L. caulleryi in the direct fluorescent antibody test suggested that antigens common to different developmental stages, or shared antigens, might exist, as well as specific antigens so far well-known. The fluorescent intensity of oocyst sporozoite, and schizont contents was apparently lower than that of any other stage. This evidence might be elucidated by the antigenic incompletion.

Animals↗

Developmental stages of the third molar in Israeli children.

PURPOSE: The purpose of this study was to examine the developmental stages of the third molar in Israeli children, compare its development in boys and girls, in the maxilla and mandible, right to left sides in various age groups, and relate the developmental stage to chronological age. METHODS: Panoramic radiographs of 693 healthy children and adolescents (328 boys and 365 girls) ages 7 to 16 were analyzed using a modified 6-stage method. Since the 16-year-old group was too small for statistical analysis, it was later excluded from the study. Radiographs were obtained from patient files. RESULTS: There were no significant differences in the development of the third molar between boys and girls and between right and left sides. Slight differences were found between the maxilla and mandible. The first appearance of a radiolucent bud was at age 8.7 in the mandible and 9.3 in the maxilla. Crown completion was observed on average at age 11.7 in the maxilla and 11.8 in the mandible. CONCLUSIONS: There was a high correlation between third molar development and chronological age. Agenesis can be determined conclusively if no radiolucent bud is present beyond age 14. The modified 6-stage method allowed more accurate determination of developmental stages.

Adolescent↗

Similar size of the replicons in Chironomus polytene chromosomes at two developmental stages.

The size of the DNA replication units in salivary gland polytene chromosomes of Chironomus arvac at 2 developmental stages (red-head and late 4th instar) was investigated. The 2 developmental stages differ with respect to intensity of DNA replication with much lower values at the red-head stage. Also the level of polytenization is lower at the red-head stage. The size of the replication units was measured by determining the size of double-stranded DNA fragments released from active replication units during the cell lysis. At both stages it is possible to release from the polytene chromosome a double-stranded DNA fragment of similar size. This indicates that there is no or very little difference in size of active replication units in the same tissue at the 2 developmental stages.

Animals↗

Effect of heat on radiosensitivity at different developmental stages of embryos of the fish Oryzias latipes.

Embryos of the fish, Oryzias latipes, at various developmental stages were heated before or after gamma-irradiation. The lethal effects of heat and radiation treatments were determined by the hatchability of the irradiated embryos. The radiosensitivity suddenly decreased at 3-days after fertilization. Heat (41 degrees C), either preceding or following irradiation, decreased the shoulder region (Dq) of the dose-response curves of the embryos irradiated at the 1-3 day stages, and increased the slope (D0) only at the 3 day stage. The magnitude of enhancement of radiosensitivity by pre- and post-heating was found to depend on the time and temperature of the heat as well as the developmental stage of the embryos; in 1- and 2-day embryos the effectiveness of post-irradiation heating was larger than that of pre-irradiation heating whereas in 3-day embryos there was no difference between the effectiveness of pre- and post-treatment. The sequence of heat (41 degrees C) and radiation treatment, and the time interval between the two treatments, also influenced the effectiveness of heat on radiosensitivity, depending upon the developmental stage; recovery from heat effects was not observed within a time interval of up to 3 h before radiation exposure for either 1- or 3-day embryos, whereas recovery from the radiation lesion occurred, for only 1-day embryos, within 1 h of subsequent heat exposure.

Age Factors↗

Quantification of vitellogenin in several developmental stages of medaka (Oryzias latipes) S-rR strain.

Concentrations of vitellogenin (VTG) in different developmental stages: embryo (on the day of fertilization); yolk-sac larva (on the day of hatching); 2, 4, 6 and 8 weeks posthatching; were determined in medaka (Oryzias latipes) S-rR strain. Both sexes were bred separately from 1 week after hatching and fed only Artemia nauplii to avoid contamination by xenoestrogen from females and feed. Whole bodies of ten test fish/group were homogenized individually and the supernatants were quantified. Quantification of VTG was performed by an enzyme-linked immunosorbent assay (ELISA). In most males, VTG levels were less than 1 microg/g body weight and no fluctuation was observed throughout the developmental stages. In 2-week-old females, five had no detectable VTG, and the others had 5.18+/-2.37 microg/g of VTG. The 4-week-old females had 16.3+/-12.0 microg/g VTG, and the concentrations increased with maturity to 5.54+/-3.09 mg/g in 6-week-old and 8.99+/-3.10 mg/g in 8-week-old specimens. These results show that the concentrations of VTG in males are routinely close to the detection limit independent of the developmental stages in an environment with low contamination by xenoestrogen derived from artificial feed and females. Females have detectable VTG levels even in the juvenile phase, and the level increases markedly with maturation.

Animals↗

Angular changes and their rates in concurrence to developmental stages of the mandibular second premolar.

In the early developmental stage of the mandibular second premolar (MnP2), it is not unusual to find the tooth extremely angulated to the lower border of the mandible, as seen in the panoramic roentgenogram. On eruption, the tooth, in most cases, is close to being upright. However, impaction or other types of malocclusions due to its ectopic eruption are not rare. This study follows the angular changes of the MnP2 during development. Two hundred two panoramic roentgenograms of 101 patients were retrospectively analyzed. All patients had two sequential films with a minimal time interval of nine months. Each MnP2 was traced, and its developmental stage as well as its angulation to the lower border of the mandible was registered. We found that normally more MnP2 are distally (56.5%) than mesially (25%) inclined. There is a statistically significant difference in the inclination of the teeth during their development from stage D to stage F (D = 75.17 degrees +/- 15.25 degrees, E = 79.35 degrees +/- 12.18 degrees, F = 83.38 degrees +/- 10.79 degrees). The average amount of total angular change rate of the MnP2s from stage D to stage G is 0.09 +/- 0.25 degrees/mo, and the absolute angular change rate is 0.19 +/- 0.25 degrees/mo.

Bicuspid↗

Vitrification of porcine embryos at various developmental stages using different ultra-rapid cooling procedures.

In this study, three different vitrification systems (open pulled straw: OPS; superfine open pulled straw: SOPS; and Vit-Master technology using SOPS: Vit-Master-SOPS) were compared in order to investigate the influence of cooling rate on in vitro development of vitrified/warmed porcine morulae, early blastocysts, or expanded blastocysts. Embryos were obtained surgically on Day 6 of the estrous cycle (D0 = onset of estrus) from weaned crossbred sows, classified and pooled according their developmental stage. A subset of embryos from each developmental stage was cultured to evaluate the in vitro development of fresh embryos; the remaining embryos were randomly allocated to each vitrification system. After vitrification and warming, embryos were cultured in vitro for 96 h in TCM199 with 10% fetal calf serum at 39 degrees C, in 5% CO(2) in humidified air. During the culture period, embryos were morphologically evaluated for their developmental progression. The developmental stage of embryos at collection affected the survival and hatching rates of vitrified/warmed embryos (P < 0.001). The vitrification system or the interaction of vitrification system and developmental stage had no effect on these parameters (P > 0.05). Vitrified expanded blastocysts showed the best development in vitro (P < 0.001), with survival and hatching rates similar to those of fresh expanded blastocysts. The hatching rate of fresh morula or early blastocyst stage embryos was higher than their vitrified counterparts. In conclusion, under our experimental conditions, cooling rates greater than 20,000 degrees C/min, as occurs when SOPS or Vit-Master-SOPS systems are used, do not enhance the efficiency of in vitro development of vitrified porcine embryos.

Animals↗

[Studies on susceptibility of Pomacea canaliculata of different developmental stages to infection with Angiostrongylus cantonensis].

UNLABELLED: OBJECTIVE To study the susceptibility of Pomacea canaliculata of different developmental stages to Angiostrongylus cantonensis infection. METHODS: P. canaliculata snails breeding at laboratory were divided into four rank (I-IV) according to the weight, and infected with the first stage larvae of A. cantonensis from Fujian Province. Their mortality, infection rate, worm burden, and the size, development and distribution of larvae in snails were examined. RESULTS: Snails at different developmental stages were readily infected with A. cantonensis. The infection rate was between 76% and 100%, with no significant difference among the groups (P>0.05). Snails at earlier developmental stage showed higher mortality. The heaviest worm burden and the largest number of snails harboring more than 100 larvae were found in snails of rank III. In general the larvae in snails showed a synchronous development in the groups. Sizes of the third stage larvae in snails of various ranks were homogeneous. The period before the third stage larva emergence and the time for a peak percentage of the larvae exhibited no significant difference among the four ranks. The larvae widely distributed in various parts of snails, with more in the lung and foot, and larvae from snails of different ranks could all infect rats successfully. CONCLUSION: P. canaliculata of the four ranks can all be infected by the first stage larvae of A. cantonensis. Rank III snails may be better for studying the relationship between P. canaliculata and A. cantonensis. The potential role of young snails in angiostrongyliasis transmission should be recognized.

Angiostrongylus cantonensis↗