[Parasitic diseases of cultivated fish].
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The Authors refer about one case of cutaneous infection from Mycobacterium marinum transmitted to man from tropical fishes of a fish tank. The Authors describe the lesions produced by mycobacterium in man and in fish, as well as the relevant hystological findings and the microbiological enquiries carried out for isolation and typing. The possibility of direct transmission of a tubercular infection from fish to man has been demonstrated. The Authors think it to be useful a careful sanitary control on the ichthyc fauna destined to domestic fish tanks.
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Investigations concerning the monocytic and lymphocytic cell series were carried out on the peripheral blood and dabbings of the kidney, the spleen and intestines of healthy eels (anguilla anguilla), and eels taken ill with vibrio anguillarum. By means of histological, cytochemical, phaseoptical, and electronmicroscopical examinations independent development lines of the monocytes and lymphocytes were established. The place of origination for the monocytes was found to be the kidney, for the lymphocytes the spleen and the intestines additionally. The evidence of the cytochemical assay of enzymes for the differentiation of these blood cells and their cellular systems is primarily dependent on the distribution of the activity of the unspecific esterase, the naphthol-AS-D-chloroacetate-esterase, the peroxidase and the alkalic phosphatase. Macrophages were - on the basis of enzymocytochemical results - seen as derivatives of the monocytes. Plasma cells and lymphoid cells are transformation forms of the lymphocytes. Our results emphasize the significance especially of cytochemistry for the hematological diagnostics of fish diseases.
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Tetrahymena corlissi, a free-living protozoan, apparently caused the death of large number of guppies (Poecilia reticulatus) and occasionally other fishes, in aquaria and hatcheries at several locations. Apparently the disease occurs when the fish and protozoan populations are both at a high level of density. The signs include white spots and epidermal damage. Histologically, T. corlissi could be seen in skin, muscle, and viscera; in some there was marked inflammation, in others there was little tissue reaction. A key is presented for the identification of invasive fish ciliates: Chilodonella, Hemiophrys, Ichthyophthirius, Ophryoglena, and Tetrahymena.
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Most disease problems affecting farmed salmonid fish are either caused or exacerbated by bad husbandry and poor environmental conditions. The aspects of water quality and nutrition which are significant in causing husbandry problems are considered. The clinical aspects of these diseases are decribed in terms of the life cycle of salmonids, and methods of prevention are discussed.
Host fishes (Boops boops) parasitized by the cymothoid isopod Meinertia oestroides show an atrophied tonuge: its length and its initial weight can decrease of about 50%. These morphological variations are connected with deep histological alterations, such as: A more or less important vanishing of epidermis. A disorganization of the connective tissue, by numerous invading blood cells. A total vanishing of cargilage and a partial vanishing of bone, resulting from the lysis of their own fundamental substance.
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Liver abnormalities were found, by gross and histopathologic examination, in 92% of the English sole (Parophrys vetulus) from the Duwamish River Estuary, Seattle, Washington. Hepatomas were found in 32% (20 of 62) of the English sole. Other observed liver aberrations included increased fatty vacuolation, congestion, structure disarray, increased size and number of melanin-macrophage centers, centrolobular fatty degeneration and necrosis, increased amounts of perivascular connective tissue, intercellular melanin deposits, and hepatocellular hypertrophy often associated with the presence of bizarre nuclei and/or multiple nucleoli. Livers evidencing microscopic lesions were usually discolored. Livers containing hepatomas were often mottled yellow or tan and brown; occasionally, hepatomas were visible as tan or white nodules. Although the cause(s) of the liver abnormalities has not been conclusively identified, chemical analyses of Duwamish River English sole have detected polychlorinated biphenyl (PCB) levels of about 1.5 ppm (dry wt) in total body tissue. Many of the above-mentioned abnormalities, with the exception of hepatomas, have been observed in fish exposed to PCB's.
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Some tissual alterations of the parasitic microbiotope are shown in fishes harbouring Cymothoidae (blood-sucking Isopods). These alterations consist mainly in a hypertrophied epidermis and a deeply disturbed conjunctive tissue; moreover, it can be observed an important production of mucus on the tegument surface and a rush of blood cells (lymphocytes, eosenophilic granulated cells) and of cells of histiocytary origin (giant multinucleated cells).