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[Observation amyloplasts in the gravity-insensitive mutant of rice under gravity and microgravity conditions].

Using histochemistry and optical microscope, we examined the number and the size of amyloplasts in specialized tissues of gravitropically receptive organs-tissues such as the coleoptile and sheath of rice mutant (insensitive to gravity) and wide type (Oryza sativa L. subsp. japonica) (Zhonghua 11). We found there was no statistical difference between the mutant and wide type, both of which grew on earth or on the clinostat respectively. On earth, it was found that amyloplasts sedimented at the distal end of each cell of the special starch sheath tissues and re-sedimentation of amyloplasts toward the direction of gravity was almost completed in 5 minutes after inverting the seedlings. On the clinostat, amyloplasts dispersed in the starch sheath tissue. Such observations indicated that the mutation was not resulted from the starch-deficiency or starch-absence, the further research is going on.

Gravitation↗

Femur-bending properties as influenced by gravity: V. Strength vs. calcium and gravity in rats exposed for 2 weeks.

Growing bone material strength (S) can increase with gravitational intensity (g). That would be consistent with demineralization reported during space flight and reports that strength increases with mineral content. This study, however, shows an increase in material strength independent of calcium content (C). Male, Sprague-Dawley rats were exposed to chronic simulations of altered gravity from the 28th to 42nd d of age. Zero G was stimulated for 13 animals by harness suspension and 3 G for 30 animals by centrifugation. For fresh femurs, S as determined by bending and C as determined by AA spectrometry were compared with results for 11 harnessed, control animals and 13 normal, control animals. Multiple regression shows significant dependence of S (10(6) N.m-2) upon g (multiples of Earth's gravity, G) as independent from C (% by mass) for which there is no significant coefficient of partial regression: S = (62 +/- 1) + (7 +/- 1 g) + (0 +/- C).

Animals↗

Statoliths motions in gravity-perceiving plant cells: does actomyosin counteract gravity?

Statocytes from plant root caps are characterized by a polar arrangement of cell organelles and sedimented statoliths. Cortical microtubules and actin microfilaments contribute to development and maintenance of this polarity, whereas the lack of endoplasmic microtubules and prominent bundles of actin microfilaments probably facilitates sedimentation of statoliths. High-resolution video microscopy shows permanent motion of statoliths even when sedimented. After immunofluorescence microscopy using antibodies against actin and myosin II the most prominent labeling was observed at and around sedimented statoliths. Experiments under microgravity have demonstrated that the positioning of statoliths depends on the external gravitational force and on internal forces, probably exerted by the actomyosin complex, and that transformation of the gravistimulus evidently occurs in close vicinity to the statoliths. These results suggest that graviperception occurs dynamically within the cytoplasm via small-distance sedimentation rather than statically at the lowermost site of sedimentation. It is hypothesized that root cap cells are comparing randomized motions with oriented motions of statoliths and thereby perceiving gravity.

Actomyosin↗