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At least 37 records · Page 2Linked to original sources
PubMed1990

Shared epitopes between the soluble proteins of Hypoderma lineatum and Hypoderma bovis first instars.

Cattle are known to acquire immunological resistance to hypodermyiasis by repeated exposure to both species of cattle grubs, Hypoderma lineatum (Villers) and Hypoderma bovis (L.). Vaccination of cattle with purified proteins of H. lineatum, particularly hypodermin A, is known to protect cattle against hypodermyiasis by this species. The development of a protective recombinant vaccine against both species using hypodermin A isolated from H. lineatum would require that immunological epitopes be shared by complementary proteins in H. bovis. The purpose of this study was to investigate the soluble proteins of H. bovis first-instars for shared epitopes with H. lineatum. Soluble H. lineatum and H. bovis first-instar larval proteins were resolved by nondenaturing polyacrylamide electrophoresis, blotted onto nitrocellulose paper, and probed with selected polyclonal cow, polyclonal rabbit, and mouse monoclonal antisera. Considerable cross-reactivity was demonstrated by antibodies in the serum of an H. lineatum-infested cow as 6 of 10 resolved H. bovis proteins were bound by the antibodies. The most common shared epitope(s) was associated with hypodermin C, a collagenolytic protease. Hypodermin A shared epitope(s) were noted on 1 prominent H. bovis band (HB1-2). Hypodermin B, a prominent protein in H. lineatum, did not appear to share epitopes with H. bovis proteins. Shared epitopes between H. bovis proteins and hypodermins A and C of H. lineatum would suggest that cross-protection of cattle against H. bovis can be expected by vaccination with recombinant proteins of H. lineatum.

Animals↗
PubMed1989

Colostral transfer of antibodies specific for Hypoderma lineatum proteins.

Antibodies to Hypoderma lineatum were transferred to calves via colostrum. The antibodies transferred in the colostrum demonstrated specificity to all the H. lineatum first-instar proteins which were resolved by non-denaturing polyacrylamide gel electrophoresis and were capable of mediating a Type I hypersensitivity reaction. The kinetic decline of colostrum-acquired antibodies, the effect upon development of an autologous humoral response to H. lineatum and the host or parasite protective role of these antibodies are discussed.

Animals↗
PubMed1975

[The larval development of the warble-fly Oestromyia leporina Pall. (Deptera, Hyodermah'dae) (author's transl)].

The present paper deals with the larval development of the warble-fly Oestromyia leporina and especially with the development of the first instar larvae (L 1). The hatching from the egg and the modus of infection is described. The migration of the L 1 within the host is reported for the first time as well as the perforation of the skin after the migration period. Exact dates on the duration of the different larval stages are given. Experimental infection reveals a partial immunization of the host against new infections. Some remaining problems of larval biology are discussed. By comparison several facts suggest our present knowledge of the perforation of larval (L 1) Hypoderma bovis probably to be wrong.

Animals↗
PubMed1975

[Cultivation of the warble-fly Oestromyia leporina pall. (Diptera, Hypodermatidae) and its biological prerequisites (author's transl)].

The warble-fly Oestromyia leporina was cultivated for four generations in the laboratory. In the present paper the technic of laboratory mating and the modus of experimental infection of the host are described. The fertility of females is tested by experiments and by dissections. It is shown how the diapause of the puparia can be shortened and how it can be omitted. The successful infection of several species of small mammals demonstrates that O. leporina can be colonized not only on the natural host but also on some common laboratory animals like mice and rats.

Animals↗
PubMed1979

[Histological reactions of the host induced by the larvae of the warble-fly Oestromyia leporina Pall. (Diptera, Hypodermatidae) (author's transl)].

The histological reactions of Mastomys natalensis induced by the larvae of Oestromyia leporina are described from the start of the infection to the complete healing of the evacuated cyst. Against the migrating larva no cellular reactions take place. After the larva settles, the most obvious feature is a non-suppurative inflammation of the surrounding tissue, while a layer of granulation tissue, infiltrated with eosinophils, is built up around the parasite. About 13 days p.i. the number of eosinophils starts to decrease again. No giant cells are present; plasmocytes and lymphocytes are relatively scarce. A few days before the parasite leaves the host, the inner surface of the warble is infiltrated with masses of neutrophils, obviously caused by secondary invasion of bacteria through the warble opening. After the parasite leaves the host, the repair of the tissue takes place within three weeks. The remaining scar tissue contains cells carrying haemosiderin, which disappears entirely about two months later.

Animals↗
PubMed1991

The response of cattle vaccinated with hypodermin A to a natural infestation of Hypoderma bovis and Hypoderma lineatum.

In this study, we have determined whether immunization with hypodermin A (HA), associated with various adjuvants, could provide protective immunity for calves when challenged with a natural hypoderma infestation. Groups of naive calves were vaccinated with HA antigen alone or with adjuvants [Freund's incomplete adjuvant (FIA) or alumina phosphate (AP)]. Subcutaneous injection with HA antigen with or without adjuvant did not significantly protect calves against a natural hypodermosis infestation. The humoral response during the infestation period was evaluated by ELISA. A significant earlier and greater response was induced in groups vaccinated with HA alone and HA combined with FIA. These results indicate that HA, in this vaccination protocol, induces a very incomplete protection in calves exposed to a natural infestation.

Adjuvants, Immunologic↗
PubMed1992

Effect of hypodermin A, an enzyme secreted by Hypoderma lineatum (Insect Oestridae), on the bovine immune system.

The absence of any inflammatory reaction around the first instar larvae (L1) of Hypoderma sp. in previously uninfested cattle suggested that these larvae may escape the non-specific defence system of the host. Immunosuppression had been noted during an experimental infestation. The aim of this work was to determine more precisely the potential role of hypodermin A (HA), an enzyme secreted by the larvae, in this immunosuppression. HA was found to have no effect on unstimulated lymphocytes from naive cattle but could influence the response of these cells to mitogens. In calves, injection of HA was accompanied by a decrease in the lymphocyte proliferative response to mitogens. This immunodepression lasted only for the duration of enzyme injections. In cattle, when HA is added, the antigen-dependent proliferative response increased significantly after 1 week of injection and disappeared 2 weeks after the end of the injection period. Finally, the rate of production of anti-HA antibodies increased at the same rate for calves and cows, and achieved a similar level. These results suggest that HA significantly modified the lymphoproliferative response for naive cattle and, to a lesser extent, immune cattle during the time of administration only.

Animals↗