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[Scanning electron microscopy studies in arthritic changes in the malleus incus joint and reflections on the function of the middle ear muscles].

The surfaces of immobilised incus and malleus joints were studied with the scanning electron microscope. The hyaline cartilage showed arthritic changes similar to osteoarthritic big joints of the body. A direct radiologic magnification technique could likewise demonstrate typical arthritic signs. Comparing findings from histologic studies in the literature, we could demonstrate that the intact hyaline cartilage degenerates if the ossicles are immobilised. The minimal static friction of the intact smooth joint surface is a prerequisite for its assumed role namely protection of the inner ear against hazardous pressure changes. A hypothesis is developed to the effect that the movement of the ossicles, necessary for lubrification and nutrition of the hyaline cartilage, is maintained by the two middle-ear muscles. Their function, which had not been explained so far could thus be interpreted--besides a possible effect on sound transmission--as preserving the intact joint surfaces of the ossicles. Nature seems to set great store by the importance of the middle ear joints, as shown by the development of a new joint structure in an implanted homoio-incus.

Adolescent

The versatility of presculptured homograft incus prostheses.

A twelve-year experience with banked homograft ossicles has developed into a system of presculptured incus allografts adapted for the correction of many ossicular defects encountered in tympanoplasty and revision stapes surgery. The standardized preparation of a number of variations of modeled incus prostheses for an operating room bank by a well-trained ear homograft laboratory technician has been an effective and time-saving technique.

Ear Ossicles

[Comparison of the results of incus interposition and artificial columella in tympanoplasty (author's transl)].

The average postoperative air-bone gap after interposition of an incus between tympanic membrane and stapes footplate is too high in case of missing crura of the stapes, it is 27 dB. The interposition of an artificial prosthesis, according to Shea and beeing made of "Platipore", leads to a statistically based lower air-bone gap with a mean of 20 dB. Concerning the change of bone conduction, there is no difference between these two methods of operation. The rate of artificial prosthesis which are perforating the tympanic membrane, increases after seven months up to 15%, but only 4% of the incuses invade the plane of the tympanic membrane after 22 months.

Bone Conduction

[Problems of autologic incus interposition in cholesteatoma operations (author's transl)].

Our clinical study proves whether or not the warning is justified not to reimplantate autologic ossicula in cholesteatoma operations in order to avoid recidives. After interposition of the autologic incus in 18 patients we found cholesteatome recidives--originating from the incus--in two cases. According to histological studies, even the most careful preparation cannot avoid that some cells of the epithelium remain on the ossicula, from which a cholesteatoma recidive may develop. Clinical studies show rather low rates of recidives compared with the histological findings. Possibly remaining squamous epithelium cells in autologic ossicles need not always cause recidives. Clinical and histological studies lead to the demand that because of the possibility of cholesteatoma recidives autologic ossicula should not be reimplated in cholesteatoma operations.

Cholesteatoma

Incus replacement prostheses of hydroxylapatite in middle ear reconstruction.

Hydroxylapatite is a calcium bioceramic that has the same chemical composition as living bone, Ca10 (PO4) 6 (OH) 2. Since 1970 it has been used as a material in reconstructive prostheses and augmentation of lost tissues in various surgical specialties including maxillofacial surgery, plastic surgery, otolaryngology, and orthopedics. For over 20 years the author has used autograft and homograft ossicles in tympanoplasty. These incudi have been modified into prostheses that were utilized in ossicular reconstruction. During this time two principle prostheses have evolved, the notched incus with short and long processes. The short process prosthesis is used with an intact stapes, whereas the notched incus with long process carries the sound pressure directly to the stapedial footplate. These prostheses have been successful in improving and maintaining hearing following tympanoplasty. Unfortunately, however, the use of human tissue has certain limitations: it is not readily accessible and has a limited shelf life. Furthermore, clinicians are wary of using homograft tissue as concern over the AIDS virus spreads. Therefore it was felt prudent to develop a manmade prosthesis that would as nearly as possible match the advantages of living bone. Hydroxylapetite most nearly met those qualifications.

Adult

Human incus long process depressions in the surface of the normal ossicle.

Depressions in the bone surface of the normal human incus long process are found with great frequency. This paper reviews the frequency and distribution of these depressions, discusses their histologic appearance, and speculates upon their significance. While their clinical significance is unclear, they may be related to several conditions encountered in otologic practice.

Ear Ossicles

[Incus, controlled deepfrozen or in cialit conserved, after allogenetic implantation. An animal-experimental study with comparing histology (author's transl)].

The histological reactions to the implantation of allogenetic incus after conservation in Cialit or after controlled deepfreezing are described. After 5 months the incusses of both conservation technics show the same good tendency of revitalisation. Therefore the controlled deep-freezing technic does not offer an advantage compared to the Cialit conservation method at least for the present state of knowledge.

Animals

Experimental autogenous incus transposition.

Incus replacement or transposition was performed in 82 rats. Findings were similar in that a degree of vitality was retained or regained, though more slowly in boiled implants. Gelatin sponge induced fibrosis. Activity rapid at first ceased later. Generally replanted includes seemed stable.

Animals

Autologous fitted incus versus Plastipore PORP in ossicular chain reconstruction.

Hearing results and causes of failure with three types of ossicular reconstruction techniques over an intact stapes, during second-stage intact canal wall tympanoplasty, are reported herein. The three types of reconstruction are: fitted autologous incus (38 cases); Plastipore PORP with cartilage (41 cases); Plastipore PORP without cartilage (32 cases). A residual air-bone gap within 15 dB. was found in 63.2 per cent of fitted includes, in 41.5 per cent of PORPs with cartilage, and in only 37 per cent of PORPs without cartilage. Eighty-four per cent fitted incudes, 63 per cent PORPs with cartilage and 44 per cent PORPs without cartilage yielded a residual air-bone gap within 25 dB. Extrusion has been the main cause of failure among Plastipore prostheses.

Biocompatible Materials

Bone pâté repair of the eroded incus.

The authors report on a series of 25 patients who, in the process of undergoing tympanoplasty, were noted to have erosion of the long process of the incus. This was repaired with bone pâté in an attempt to restore ossicular continuity. The methods and results are presented and the prospects for the future are discussed.

Ear Diseases

A five year follow up of incus transposition in relation to the first stage tympanoplasty technique.

This study was set up, prospectively, to determine factors affecting the long-term hearing results of patients undergoing incus transposition as a second stage in ossicular reconstruction, following a successful drumhead repair in non-cholesteatoma ears. Seventy-one patients were entered into the study over five years from 1980-1985, 66 were available to be studied throughout the five year follow-up period. Nine weeks post-operatively, 74 per cent of all patients has an air-bone gap of less than 15 dB (48/66). The type of first stage procedure had a significant effect on the hearing levels in the final five year assessment. The most successful sub-group were those patients who had a cortical mastoidectomy and silastic sheeting inserted in the first staging procedure. The air-bone gap, of less than 15 dB, was maintained in 71 per cent of this group (17/24). The sub-group who had a simple myringoplasty as the primary procedure had a good initial hearing level. By five years, however, only 30 per cent of the patients had maintained the air-bone gap of less than 30 dB (3/11).

Chronic Disease

Histological changes of incus with cholesteatoma in the attic.

In 160 cases of chronic otitis media with cholesteatoma in the epitympanum and intact ossicular chain, histological analysis of the incus was performed. The ossicles were cut in serial sections and every five cuts analysed. The findings were normal in 10.7%, a fibrous thickening of the periost was found in 13% of the cases. In 8% of the cases there was fibrosis of the periost with foci of active inflammation. Ostitis or osteomyelitis in the bone itself was present in 67.5%. In conclusion, removal of the ossicle during tympanoplasty was deemed necessary in 67.5% of the cases since otherwise relapse of the inflammatory process could sooner or later be expected.

Cholesteatoma

[Histological study of otological homografts (incus, cartilage and fascia) (author's transl)].

To summarise our morphological findings of this study the following conclusions are drawn: In bone grafts growth of vital cells originating from the Haversian canals is clearly demonstrated. The growth of connective tissue in the Haversian system is understandable on general principles of pathology. The morphology of the change of osteocytes from fibrocytes through osteoblasts is photographically clearly shown and is in keeping with the findings of Steinbach and of Kerr and Smyth. New bone formation was detected at earliest nine months after implantation. Our investigations do not confirm whether this new bone formation occurs persistently in all cases or to what extent the original homograft material, preserves in cialit solution, takes part in this process. A similar mechanism of tissue regrowth should be accepted in homograft cartilage. We have studied two cases, one after 6 months and the other after 5 years following implant. The three fascial grafts examined compare with earlier publications, with oedema, slight inflammation and little shrinkage occurring. The suitability for grafting of homograft incus, cartilage and fascia we believe to have been demonstrated.

Adult

On the oscillation behavior of the incus-stapes joint (author's transl).

A two-channel inductive measuring system in the human temporal bone preparation was used to oscillographically record oscillations of incus and stapes as well as to observe the phases between the two auditory ossicles at the same time. In the frequenzies between 300 and 1,200 Hz it was not possible to prove clearly a phase difference, in the higher frequency ranges (approximately from 2,000 Hz upwards), however, a phase difference was observed to occur regularly to an extent of 90 degrees and more.

Ear Ossicles

[Fixation of the malleus and incus].

During the past five years at the ENT Clinic in Olomouc 15 incudomallear synostoses of inflammatory aetiology were observed; 10 times tympanotomy was found, performed in patients without an inflammatory history, fixation of the malleus, or fixation of the malleus and incus or fixation of the stapes and malleus. The authors demonstrate three typical cases, incl. preoperative and postoperative audiograms. As during operation the ossicles are mobilized and left in situ et in function or removed by milling and destroyed and replaced by a reconstruction, the authors demonstrate a primarily fixed malleus found accidentally in the skull from medieval excavations, and histological preparations of primary incudomallear synostosis from a macerated skull in the anatomical museum.

Ear Diseases

Osteoma of the incus.

Osteomas of the temporal bone are rare. Osteomas of the middle ear have been reported but none involving an ossicle. We describe a case of a 28-year-old man who had a conductive hearing loss caused by an osteoma of the incus.

Adult

[The histological changes in cartilage (incus) in the middle ear after conservation--an animal study (author's transl)].

This study demonstrates the histological changes of incus cartilage after transplantation into the middle ear space of rabbits. Conservation was performed by deep-freezing, lyophilisation in connection with gamma-irradiation, or with Cialit in differing dilutions. Controlled deep-freezing at about -196 degrees C and uncontrolled deep-freezing at about -20 degrees C guarantee after transplantion the survival of some vital chondrocytes. The other methods of conservation cause death of all chondroxytes. Cartilage prepared by these methods demonstrates potential for new bone formation. Only the xenogenetic cartilage loses its osteogenic potential after treatment by lyophilisation in connection with gamma-irradiation. The histological value of the transplant differs according to the method of conservation. No special tendency for resorption or inflamatory reactions could be seen.

Animals