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PubMed1986

[Morphologic study of microscopic hematuria using phase contrast microscopy].

Phase contrast microscopic examination of the urine has been recently reported to be valuable in predicting whether hematuria is due to glomerulonephritis or not. Phase contrast microscopy can differentiate glomerular red cells from nonglomerular ones since the former varies in size and shape with distortion, whereas the latter is uniform in size and shape. Urinalysis of 217 cases of microscopic hematuria was performed at our Hospital. Red blood cells were classified into two types, glomerular and nonglomerular type. Ninety percent of the cases of bladder leukoplakia as well as all cases of glomerulonephritis had glomerular type red blood cells.

Diagnosis, Differential↗
PubMed1975

Use of peroxidatic-enzyme staining to enhance resolution of cultured mammalian cells under phase microscopy.

Staining of glutaraldehyde-fixed mammalian cells with peroxidatic enzymes (horseradish peroxidase or horse heart cytochrome c) greatly enhances resolution of their structure under phase microscopy. The topography of cell processes and regions of intercellular contact and overlapping is resolved precisely, even in dense cultures mounted in media which ordinarily do not permit clear demonstration of these areas. The technique is therefore a useful aid to the study of cultured cells with phase optics. Labeling depends on introducing free aldehydes into cells through the use of bifunctional fixatives such as glutaraldehyde. Acetone or formaldehyde fixation prevents staining, and labeling intensity is greatly diminished by pretreatment with spermine, a polyamine that reacts with glutaraldehyde. Electron microscopy reveals that peroxidase tags membranes preferentially; some areas are labeled smoothly, others in a punctate manner. Ribosomes are sharply contrasted, but nuclei remain unstained. Cytochrome c labels condensed nuclear chromatin intensely, and also stains ribosomes and portions of the cytoplasmic ground substance; membranes are mostly unmarked.

Animals↗
PubMed1962

The origin of protein and fatty yolk in Rana pipiens. I. Phase microscopy.

Study of living frog oocytes with the phase microscope has shown that the early yolk appears in two forms. One of these, the protein yolk, consists of thin, dense, plate-like bodies which in face view are almost always regular hexagons. The other form, the fatty yolk, occurs as clusters of globules of varying sizes. The plate-like bodies occur both singly and in clusters. As the oocytes mature these plate-like bodies grow in size while retaining their hexagonal outline. Mitochondria have been observed to increase in length and numbers as the oocytes mature; they are rods or filaments at all stages of growth up to an oocyte diameter of 300 microns. The oocyte cytoplasm gradually becomes packed with long mitochondria, plate-like bodies, and clusters of globules.

Animals↗