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At least 37 records · Page 2Linked to original sources
PubMed1977

Scanning electron microscopy of human female reproductive tract and amniotic fluid cells.

Scanning electron microscopy was used to examine surface ultrastructural characteristics of cells of the epithelium of female reproductive tract, cervical mucus, and amniotic fluid cells. The female epithelium undergoes hormone-dependent cyclical morphological alterations in cell shape, apical microvilli, ciliation, and secretory patterns. The frequency distribution of ciliated cells varies in different parts of the female reproductive tract and different segments of the same organ. In the endocervix, a few ciliated cells are randomly distributed. The density of ciliated cells is variable in several areas of the endometrium. The sparse distribution of ciliated cells in the central portion of the uterine wall may indicate a possible implantation site. According to surface ultrastructure of cells, three segments are noted in the uterotubal junction: cornual endometrium, interstitial tubal epithelium, and transitional area. The vaginal epithelium is made of flat polygonal cells with interdigitating borders and a fine interlacing network of microridges. After the menopause, the microridge pattern and distinct cell borders are lost or severely modified. Two types of fibrous structures can be found in cervical mucus: a) microfibrils that vary in diameter from 500 to 1,500 A and from bundles or networks, and b) long, thick fibers that vary in diameter from 0.5 to 5 micrometer run parallel to each other, and appear to be made up of microfibrils. Amniotic fluid cells, derived from amniotic epithelium and fetal ectoderm, consist of non-nucleated and nucleated cells with extensive microplicae.

Amniotic Fluid↗
PubMed1975

Histochemistry and scanning electron microscopy of tooth integumental material in humans.

Plaque material on human teeth was investigated using histochemical techniques and scanning electron microscopy. A positive reaction for protein, cargohydrate, and lipids was seen around the fissures and in a well-defined band around the sides of the tooth corresponding to the highest contour. These regions are the location of dense aggregates of bacteria as seen with scanning electron microscopy. This study emphasizes the fact that considerable attention should be given to the cleaning of not only the occlusal surfaces but also the highest contour of the tooth.

Adolescent↗
PubMed1977

[Scanning electron microscopy of cystoid degeneration in the peripheral retina (author's transl)].

Scanning electron microscopy of peripheral cystoid degenerations of the retina as well as of reticular or inner cystoid degenerations shows tunnel-shaped and vault-like cavities which are supported by columns. In marked cases of PCD, fibers of a collagenous nature surround and connect the columns. Inside the columns there are cable-like strands, which are probably stretched neuronal processes. The surface covering of the columns is formed as a porous layer. The columns of the reticular cystoid degeneration are thinner and located in processes of the outer columns. Globular structures persent in both systems of are interpreted as deformed erythrocytes.

Erythrocytes, Abnormal↗
PubMed1978

[Observation of Schistosoma mansoni by scanning electron microscopy].

The integumental surface of adult Schistosoma mansoni was studied by scanning electron microscopy (SEM) at 220 to 10,000 magnifications. SEM shows certain basic features such as spines in the oral sucker and the acetabulum which may facilitate rasping and attachment of the parasite to stay in the bloodstream of the definitive host. It seems likely that SEM visualization will be a means for differentiation some species of the genus Schistosoma.

Animals↗
PubMed1978

Scanning electron microscopy of epithelial cells grown on enamel, glass and implant materials.

The purpose of the present study was to examine with scanning electron microscopy gingival epithelial cells grown in cell culture on tooth enamel, glass, Vitallium, titanium and vitreous carbon. SGL (Smulow-Glickman) gingival epithelial cells were grown for 5 days and processed using a critical point drying apparatus. Scanning electron microscopy carried out at X 1500 magnification revealed that the gingival epithelial cells gres equally well on all materials on either smooth or rough (sand-blasted) surfaces.

Carbon↗
PubMed1976

Scanning electron microscopy of cell surfaces following removal of extracellular material.

The application of scanning electron microscopy to the study of cell surfaces is limited in intact tissues, because extracellular material may often obscure the details of nonluminal surfaces. To remove connective tissue elements we have treated human skin and both kidney, and an autonomic ganglion of the rat with hydrochloric acid and collagenase. Regional variations in the basal surface of the nephron are noted following removal of the basement membrane. The basilar interdigitations of the cells of the proximal tubule appeared as parallel ridges encircling the tubule. Ridges on the parietal epithelium of Bowman's capsule were randomly arranged and alternated with smooth surfaces. The dermal surface of the human epidermis has an alveolar or honeycomb appearance due to the elevation of the epidermal ridges and numerous pits for the dermal pegs. At higher magnifications the basal surface of cells of the stratum germinativum possessed numerous and irregular projections. Neurons with their processes are evident in the autonomic ganglion. The soma of the neurons are enclosed by flattened satellite cells. Irregular spaces between opposed satellite cells are interpreted as regions for the passage of processes related to the ganglion cells. Nodes of Ranvier were clearly seen along nerve fibers. Some pitting of the nerve fibers was also noted. The HCl-collagenase method has the advantage of the removal of collagen and basement membrane while preserving the structural integrity of the cell surface.

Animals↗
PubMed1977

[Findings on retinal surface by scanning electron microscopy. II. Vitreous detachment (author's transl)].

The authors describe their findings by scanning electron microscopy of the surface of the retina following secondary and primary senile posterior vitreous detachment. These findings are correlated with those of transmission electron microscopy. Extended nets formed by vitreous fibrils can be found on the retinal surface. The fibrils form a continuous feltwork, which cannot be traced by biomicroscopy or dissection of enucleated globes. This feltwork desintegrates in places or over a wide area and the basal membrane of the Müller cells becomes exposed. Structural differences in consequence of the two examination techniques well as possible artifacts are shown.

Age Factors↗
PubMed1976

Scanning electron microscopy of myeloma cells.

The surface structure of myeloma cells was examined by scanning electron microscopy. The cells were collected from the pleural effusion of a multiple myeloma patient and purified by Conray-Ficoll gradient sedimentation. The cell size ranged from 8 mu to 12 mu in diameter and the microvilli were from 0.8 mu to 1.2 mu in length. The surfaces of the majority of the observed myeloma cells were more villous than lymphocytes.

Aged↗
PubMedDate not supplied

[The proventriculus of the Xenopsylla cheopis flea studied by scanning electron microscopy].

The proventriculus of the flea X. cheopis was studied by means of scanning electron microscopy. The external surface of the proventriculus is a rigid structure with an alveolate surface ("wasp nest") formed by muscular cords. The internal surface of the proventriculus is represented by numerous acanthae which fall close together. They have a shape of bent dens with longitudinal sharply angular edges and concave spaces between them. Some edges are crenate.

Animals↗
PubMed1979

Normal and pseudorabies virus infected primary nerve cell cultures in scanning electron microscopy.

Primary cell cultures from the central nervous system of the embryonic rat were inoculated with pseudorabies virus. Their morphological changes were studied by phase contrast microscopy and by scanning as well as by transmission electron microscopy. Uninfected cultures display two distinct cell layers in scanning electron microscopy: a flat continuous monolayer supports a heterogeneous population of individual, presumably neural cells, which emit processes of different number and size. The latter cells form contacts by a dense network of fibres. Infectious virus is propagated in these nerve cell cultures with similar effectivity as in other cultures. The infectoin leads to fusion and death of the cells. By the time the cytopathic effect is visible, nearly all cells, including those of neuronal and those of nonneuronal appearance, are studded with ample amounts of virus-sized particles. The particles represent viruses as demonstrated by transmission electron microscopy or by treatment with a hyperimmune serum directed against pseudorabies virus structural components. Hyperimmune serum leads to clustering of the particles at the cell surface. The amount of virus particles per surface unit was about 10 times higher on neural cells as compared to primary rabbit kidney cells. The concentration of infectious particles in the supernatant, however was approximately the same. The system described appears to be useful for the study of acute virus effects on neural tissue under strictly controlled conditions.

Animals↗
PubMed1977

Comparison of tracheal histopathology and scanning electron microscopy of infectious laryngotracheitis.

Samples of trachea were examined with light microscope, fluorescent microscope, and scanning electron microscopy (SEM). Negative controls and infectious laryngotracheitis (ILT) positive samples were compared. Histopathology illustrated lesions characteristic fo ILT. Lesions seen with SEM suggested surface changes including ciliary disruption. luminal debris, epithelial slough, crevices, hemorrhage and exudate. Fluorescence was noted in infected tracheas throughout both trials using fluorescent antibody (FA) technique.

Animals↗
PubMed1975

Scanning electron microscopy of acarus scabiei.

Details in the anatomy of sarcoptes scabiei var. hominis are described. Scanning electron microscopy is of help in illustrating this parasite which is commonly seen in clinical practice. The technique also provides the possibility to differentiate between various types of mite.

Acari↗
PubMed1979

Characterization of different regions of the normal human urinary bladder by means of scanning electron microscopy.

A study of the normal human urinary bladder and the proximal urethra was performed using scanning electron microscopy (SEM). Biopsies were evaluated from 27 male patients with benign prostatic hyperplasia, without history or sign of urinary tract malignancy and with cystoscopically normal mucosal finding. The specimens were obtained from the dome, the side-walls and the trigone of the bladder. The characteristic surface architecture of the transitional epithelium was observed in different anatomical regions of the human urinary bladder.

Aged↗
PubMed1975

Studies on leukemic cells in a patient suffering from exfoliative dermatitis (due to chronic lymphatic leukemia), especially by scanning electron microscopy.

Lymphocytes (T cells and B cells) of a patient with chronic lymphatic leukemia (CLL) were studied immunologically and scanning electron microscopically. The subpopulation percentage of rosette forming cells (T cell) and surface immunoglobulin bearing cells (B cell) were decreased in the peripheral blood. Moreover, tritiated thymidine uptake by lymphocytes in the cell culture stimulated by phytohaemagglutinin (PHA), porkweed mitogen (PWM) and purified protein derivatives (PPDs) was decreased. Scanning electron microscopy (SEM) showed many long villi on the surface of the lymphocytes in the peripheral blood, which implies B cell origin, Observation by electron microscopic immunohistochemistry using the ferritin antibody technique revealed that beta1C/beta1A receptors normally seen on the B cells were also seen on the leukemic lymphocytes. Thus it appears that the leukemic cells in the peripheral blood of this patient are functionally and morphologically abnormal B cells.

Adult↗
PubMed1979

Study of Biopsies from lesions of the oral mucosa by scanning electron microscopy (S.E.M.).

Surface epithelial cells from 55 biopsies including lichen planus, leukoplakia and squamous cell carcinoma were studied by scanning electron microscopy. The surface structures of the various oral lesions demonstrated morphological differences. After more information has been collected, the method of SEM may be of value for the recognition of pre- and early neoplastic lesions in the oral cavity.

Biopsy↗